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Citations for Thermo Fisher :
1401 - 1450 of 10000+ citations for Cyclic AMP Direct ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... The amount of TNF-α and IL-6 in serum was quantified using commercial mouse ELISA kits (Thermo Fisher Scientific). For ex vivo experiments using splenic myeloid cells ...
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bioRxiv - Pathology 2021Quote: ... After staining with 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific, San Jose, CA, US), mean absorbances in duplicates were read at 450nm and ratio of HAA/PNA was calculated for each sample in order to measure changes of glycosylation ...
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bioRxiv - Immunology 2022Quote: ... and the assay was developed with 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Fisher, Cat#34029) and blocked with 2N H2S04 solution before reading ...
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bioRxiv - Neuroscience 2023Quote: ... the substrate of the peroxidase (100µL, 3,3’,5,5’-tetramentylbenzidine solution; 1-StepTM Ultra TMB-ELISA, 34029, Thermo Scientific) was added in each well for 10 to 25 minutes at RT and in darkness ...
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bioRxiv - Microbiology 2020Quote: ... diluted 1:3000 (PBS milk 1% -Tween 0.1%) for 1 h at 25°C and detected using 1-Step(tm) Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific, Waltham, MA). Plates were read using Cytation5 (BioTek ...
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bioRxiv - Cell Biology 2023Quote: ... 96-well ELISA plates were coated with coating buffer (Na2CO3 0.1 M pH 9.6) containing IL-12p70 coating antibody (Invitrogen, clone 20C2 M122 and used at 3 μg/ml) overnight and at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... Amplicons were pooled and normalized using the SequalPrep Normalization Plate Kit (Invitrogen). Aliquots of the pool were then column (MinElute PCR Purification Kit ...
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bioRxiv - Microbiology 2021Quote: ... Amplicons were purified with the SequalPrepTM Normalization Plate Kit (ThermoFisher Scientific, USA) and then pooled and concentrated with Amicon® Ultra 0.5 mL filters (EMDmillipore ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were then normalized with SequalPrepTM normalization plate kit (ThermoFisher Scientific) and pooled ...
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bioRxiv - Microbiology 2020Quote: ... normalized by the SequalPrepTM Normalization Plate (96) kit (Invitrogen Ltd., Paisley, UK), pooled in equimolar concentrations and concentrated using the DNA Clean & Concentrator™-5 Kit (Zymo Research ...
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bioRxiv - Genomics 2021Quote: ... Amplicons were normalized using the SequalPrepTM Normalization Plate Kit (ThermoFisher, ID: A1051001), then pooled and concentrated using AMPure XP (Beckman Coulter ...
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bioRxiv - Genomics 2021Quote: ... Amplicons were normalised using SequalPrep Normalization Plate Kit (ThermoFisher Scientific, Massachusetts, US). Normalised amplicons were pooled at equal volumes into a single tube ...
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bioRxiv - Microbiology 2022Quote: ... The PCR products were normalized with the SequalPrep Normalization Plate Kit (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... on plates coated with recombinant human collagen I (Coating Matrix kit, Gibco) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... amplicons were purified and normalized with the SequalPrep Normalization Plate Kit (Invitrogen) following the manufacturers’ instructions ...
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bioRxiv - Microbiology 2023Quote: ... Amplicon levels were standardised using the SequalPrep Normalization Plate 96 Kit (Invitrogen Corporation ...
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bioRxiv - Immunology 2021Quote: Bone marrow B factions were sorted and calcium influx was assayed with a Fluo-4 Direct™ Calcium Assay Kit (ThermoFisher cat# F10471). Briefly cells were incubated with Fluo-4 Direct™ at 37°C for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... CyBio® FeliX liquid handling robot (Analitik Jena) was used to perform mRNA extraction from the resulting lysate using a Dynabeads™ mRNA DIRECT™ Purification Kit (ThermoFisher, #61012) and a custom protocol (Pallares et al. ...
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bioRxiv - Plant Biology 2021Quote: ... mRNA was extracted from the FANS-isolated VC nuclei and SC nuclei of control and TL-10/+ (about 106 nuclei per sample) with independent biological replicates using a Dynabeads™ mRNA DIRECT™ Micro Purification Kit (Life Technologies). Total RNA of late microspore (about 106 microspores per sample ...
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bioRxiv - Genetics 2022Quote: ... PCR was done to confirm the presence of the construct using F-130WH Phire Plant Direct PCR Kit (Thermo Fisher Scientific, Waltham, Massachusetts) and Sanger sequencing.
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bioRxiv - Genomics 2021Quote: ... poly(A)+ RNA was extracted by incubating 100 µg total RNA with 200 µl Dynabeads solution (Dynabeads mRNA Direct Purification Kit, Thermo Fisher Scientific, 61012) and purified following manufacturer’s protocols.
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bioRxiv - Immunology 2021Quote: ... Total mRNA was extracted from 190 pMHC-tetramerpos purified (Supplementary Figure 1A) virus-specific T-cell populations(37) using magnetic beads (Dynabead mRNA DIRECT kit; Invitrogen, Thermo Fisher Scientific). Ten μl (~1μg ...
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bioRxiv - Genetics 2022Quote: ... All amplicons were screened for sequence variations by direct sequencing using Big-Dye Terminator v3.1 sequencing kit (Applied Biosystems, Foster City, CA, USA) and ABI 3130 Genetic Analyzer (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA sequencing library was generated from 200 ng total RNA using Dynabeads® mRNA DIRECT™ Micro Purification Kit (Thermo Fisher Scientific) for mRNA purification followed by NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England BioLabs ...
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bioRxiv - Microbiology 2023Quote: ... 100 ng of RNA from each sample was then reverse transcribed into cDNA using SSIV Cell Direct cDNA Synthesis kit (lot# 00916637, Invitrogen, ThermoFisher Scientific, Vilnius, Lithuania). Subsequently ...
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bioRxiv - Cancer Biology 2023Quote: 15,000 CD133+ were FACS sorted into 40 μL of lysis binding buffer and mRNA was isolated using Dynabeads mRNA DIRECT Purification Kit (Thermo-Fisher scientific; cat# 61012) according to manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... Total protein concentrations were then assessed using Direct Detect (ThermoFisher Scientific) as per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... electron microscope equipped with a Falcon3 direct electron-counting camera (ThermoFisher). Cryo-EM data were acquired with EPU software (FEI ...
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bioRxiv - Neuroscience 2020Quote: Single RAGP neurons in lysis buffer (Cells Direct Lysis Buffer, Invitrogen) were processed for reverse transcription using SuperScript VILO Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... qPCR was performed by direct dye binding (SYBR Green, Applied Biosystems). Primers were designed using Primer3 and blasted on the NIH website (https://www.ncbi.nlm.nih.gov/tools/primer-blast/ ...
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bioRxiv - Biochemistry 2022Quote: ... electron microscope equipped with a Falcon3 direct electron-counting camera (ThermoFisher). Cryo-EM data were acquired with EPU software (FEI ...
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bioRxiv - Neuroscience 2021Quote: ... or direct method using Alexa Fluor Dyes (ThermoFisher Scientific, Waltham, MA). Nuclei were counterstained using 4′,6-diamidino-2-phenylindole (DAPI) ...
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bioRxiv - Neuroscience 2020Quote: Single RAGP neurons in lysis buffer (Cells Direct Lysis Buffer, Invitrogen) were directly processed for reverse transcriptase reaction using SuperScript VILO Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... equipped with Falcon II direct electron detector (Thermo Fisher Scientific, USA) and Cs image corrector (CEOS ...
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bioRxiv - Microbiology 2022Quote: ... equipped with a Falcon 3 direct electron detector (Thermo Fisher Scientific) for cryo-ET data collection ...
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bioRxiv - Cell Biology 2022Quote: ... equipped with a Falcon 3EC direct electron detector (Thermo Fisher Scientific). The autogrids were rotated 90° between Aquilos-2 and the respective electron microscope to optimize the relationship between the tilt-axis and the lamella orientation ...
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bioRxiv - Molecular Biology 2023Quote: ... 10µL 2x Phire Tissue Direct PCR Master Mix (ThermoFisher Scientific, USA) was used ...
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bioRxiv - Evolutionary Biology 2023Quote: ... transformed isolates were genotyped by Phire Plant Direct PCR (Thermo Scientific) using primers described in S7 Table ...
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bioRxiv - Biophysics 2023Quote: ... equipped with a direct electron detector (Falcon IV, Thermo Fisher Scientific). Samples of desired ice thickness were used for data collection in a 300 kV electron-microscope (Titan Krios G3i ...
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bioRxiv - Biophysics 2023Quote: ... equipped with a Falcon 4 direct electron detector (Thermo Fisher Scientific) and a Gatan BioContinuum energy filter ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Calcium mobilization was measured using Fluo-4 Direct calcium dye (Invitrogen). HEK293 cell lines stably transfected with 5-HT2A ...
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bioRxiv - Neuroscience 2024Quote: Cells were harvested by direct lysis in TRIzol Reagent (Life Technologies). RNA isolation was performed via phase separation according to manufacturers’ protocol ...
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bioRxiv - Synthetic Biology 2022Quote: ... Selective plates contained 15 g L-1 Noble agar (Affymetrix) to eliminate bacterial growth on carbon source contaminants available in the agar ...
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bioRxiv - Biochemistry 2019Quote: ... 1 mM DTT using Zeba spin desalting plates (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: Cell lysates used in immunoblotting analyses were prepared by direct lysis of drug-treated and untreated cells in 1× LDS–PAGE loading buffer (Thermo Fisher) after removal of cell culture media ...
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bioRxiv - Neuroscience 2022Quote: ... Live cultures or thawed cells were either expanded as floor plate progenitors (following Brundin approach; Floor Plate Progenitor Kit expansion protocol (ThermoFisher; A3165801) or terminally differentiated to DA neurons with all prior factors except in neurobasal base medium after day 25.
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bioRxiv - Immunology 2023Quote: ... A 10 μl supernatant sample was mixed with 90 μl substrate solution containing 0.5 mg/ml of 4-methylumbelliferyl N,N’-diacetyl-β-D -chitobioside in an assay buffer provided as part of the kit and in a 96-well plate (black/clear bottom plate, Thermo Fisher). The reaction was incubated at 37 °C for 30 min and then stopped by adding 100 μl stop solution ...
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bioRxiv - Developmental Biology 2022Quote: ... (shown in Figure 6E,F) were cloned to pDEST-pGADT7 (Rossignol, 2007) and pDEST-pGBKT7-Amp (Galletta, 2014) using Gateway cloning system (Life Technologies) and transformed into Y187 and Y2HGold strains ...
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bioRxiv - Neuroscience 2022Quote: ... Mobile phases A and B were with 0.1% formic acid in water and 0.1% formic acid (Fisher Scientific; Cat#: A11710X1-AMP) in ACN (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The PCR touchdown protocol was applied in a Mini Amp Plus thermal cycler (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA) as follows ...