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Citations for Thermo Fisher :
1451 - 1500 of 10000+ citations for Human integrin alpha 5 beta 3 His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... affinity purified using C-tag affinity resin (Thermo Fisher) using an AKTA chromatography system (GE Healthcare) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 0.1 µl Dream Tag DNA polymerase (Thermo Scientific, USA), 4.9 µl H2O ...
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bioRxiv - Immunology 2020Quote: ... tags were cleaved with addition of HRV3C protease (ThermoFisher) (1% wt/wt ...
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bioRxiv - Cancer Biology 2022Quote: ... an anti-6xHis-tag monoclonal antibody (Thermo Fisher Scientific) at 50 μg/mL was conjugated via amine linkage to two parallel flow cells on the surface of a CM5 chip at ≈ 10,000 response units (RUs) ...
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bioRxiv - Biophysics 2019Quote: ... The histidine tag was cleaved using EKMax enterokinase (Invitrogen), and the resulting ComR dialyzed into PBS pH 7.4 ...
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bioRxiv - Physiology 2019Quote: ... Tandem Mass Tag (TMT; Thermo Scientific, Waltham, MA, USA) labeling was performed according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Antigens were purified on C-tag column (Thermo Fisher) and quality-controlled by SDS-PAGE and for their level of endotoxin ...
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bioRxiv - Biochemistry 2022Quote: ... anti-TAP tag (1/10,000, Thermo Fischer, Invitrogen, CAB1001), monoclonal anti-Dpm1 (1/2,000 ...
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bioRxiv - Molecular Biology 2023Quote: ... The primary antibody (6xHis tag monoclonal antibody; Invitrogen/ThermoFischer), secondary antibody (Polyclonal rabbit anti-mouse immunoglobulins/horseradish peroxidase ...
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bioRxiv - Cancer Biology 2023Quote: Tandem mass tag (TMTpro) isobaric reagents were from ThermoFisher Scientific (Waltham ...
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bioRxiv - Cell Biology 2022Quote: ... the c-Myc tag (Invitrogen, clone 9E10, MA1-980), or LRP6 (Cell Signaling ...
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bioRxiv - Cancer Biology 2023Quote: ... Peptides were labeled with TMTpro isobaric tags (Thermo Scientific), pooled ...
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bioRxiv - Immunology 2023Quote: ... Antibody against 6xHis tag (MA121315) was purchased from Invitrogen. Antibodies against FLAG tag (F3165) ...
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bioRxiv - Microbiology 2023Quote: ... against the V5-tag (ThermoFisher, 46-0705, 1:1000), Flag-tag (Proteintech ...
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bioRxiv - Developmental Biology 2023Quote: ... HA Tag polyclonal antibody (Invitrogen, #71-5500; 1:3000), and Phospho-Drosophila p70 S6 Kinase (Thr398 ...
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bioRxiv - Neuroscience 2023Quote: ... Anti-V5 Tag (IF: 1:500, Invitrogen, R960-25); Anti-Adenylate Cyclase 3 (IF ...
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bioRxiv - Biochemistry 2023Quote: ... mouse anti-GFP Tag (Thermo Fisher Scientific, #MA5-15256); rabbit anti-pPKA substrate (Cell Signaling Technology ...
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bioRxiv - Biophysics 2023Quote: ... mouse monoclonal anti-V5 tag (Thermo Fisher, #R960-25) diluted at 1:5000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Anti-V5 tag monoclonal antibody (Thermo Fisher Scientific, R96025), HRP-anti-V5 Tag monoclonal antibody (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated with anti-HA Tag (Invitrogen, 26183) or anti-FLAG Tag antibody for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... anti-sense, 5’ AAGAGGUUUAGCUGGUAC CTT 3’) or control nontargeting siRNA (Genepharma, Shanghai) using Lipofectamine RNAiMAX (Invitrogen). Six hours after transfection ...
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bioRxiv - Bioengineering 2019Quote: Cells were loaded with the Ca2+sensitive fluorescence indicator Fluo-3/AM (5 µM, Life Technologies) together with 0.1% Pluronic f-127 (Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... Samples were injected onto a Biobasic AX LC column (5 μm, 50 × 3 mm; Thermo Scientific). The mobile phase consisted of 100 mM ammonium carbonate (A ...
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bioRxiv - Genomics 2020Quote: ... and fragmented by heating at 94 °C for 3 min in 5× First Strand Buffer (Invitrogen). The fragmented mRNAs were reversely transcribed into cDNA by SMARTScribe reverse transcriptase (TaKaRa) ...
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bioRxiv - Cancer Biology 2020Quote: The 3’ & 5’RACE products were cloned using TOPO TA Cloning Kit (Invitrogen, Cat #K4530-20), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... passage 3 fibroblasts were trypsinized 5 min at 37°C using 0.05% trypsin with EDTA (Gibco), centrifuged at 1000 rpm for 5 min at RT and resuspended in HBSS (Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... DM-3 and RS-5 cells were cultured in NCTC-109 medium (Gibco, Carlsbad, CA, USA) supplemented with antibiotics ...
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bioRxiv - Cell Biology 2022Quote: ... C12 (4,4-Difluoro-5-Methyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (Invitrogen) at 2 mg/ml in PBS for 10 min or with Laurdan (see above ...
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bioRxiv - Genomics 2020Quote: ... commercially available CRISPR control targeting HPRT (IDT) and KANK1 exon-targeting (ThermoFisher Scientific, 5’-GUCUAGUUGAUAACCAUAGG-3’) gRNAs were also obtained ...
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bioRxiv - Molecular Biology 2021Quote: ... Coverslips were washed 3 x 5 minutes in PBST and then incubated with secondary antibodies (Invitrogen) diluted in PBST + 0.2% BSA for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2021Quote: A 3 or 5 wt% solution of GelMAL was prepared by dissolving GelMAL in PBS (Gibco Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... cells were transfected with siRNA (5’-GGACTGAGGUUGUCAAGAA-3’) for PRC1 using Oligofectamine (Life Technologies, Carlsbad, CA) as previously described (Udy et al. ...
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bioRxiv - Neuroscience 2022Quote: ... washed 3 × 5 min with TBS and embedded in ProLongTM Gold Antifade Mountant (Thermo Fisher Scientific). Images were taken with a Nikon Eclipse90i microscope and acquisition was performed with the NIS Elements software (Version 5.01) ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 nM (final concentration) shRNA or 3 ∼ 5 µg plasmid in 50 µL Opti-MEM (Gibco) was mixed with 5 µL transfection reagent (Lipofectamine ...
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bioRxiv - Microbiology 2022Quote: ... Amplifications were achieved using a 3-step program on a QuantStudio 5 system (Thermo Fisher Scientific). Transcript abundance was calculated using the ΔΔCT method (Livak and Schmittgen ...
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bioRxiv - Microbiology 2022Quote: ... PV-3 SC8 VLPs or infectious PV-1 were incubated with 5 µM SYTO9 (Thermo Fisher) and SYPRO red (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... Data analysis was performed with the Quantstudio 3&5 Design and analysis software (Applied Biosystems, v1.5.1). Cq values were obtained via automated threshold determination ...
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bioRxiv - Neuroscience 2023Quote: ... PBS washes (3 × 5 min) and incubation with primary antibody (mouse anti-GFP, A11120, Life technologies; 1:200 ...
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bioRxiv - Molecular Biology 2022Quote: ... qPCR was carried out on a QuantStudio 3 or QuantStudio 5 Real-Time PCR System (ThermoFisher) with a passive reference of ROX using the following cycling conditions ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were passaged approximately every 3-5 days (70-85% confluency) using Accutase (Gibco, #11105-01) to detach cells ...
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bioRxiv - Neuroscience 2023Quote: ... PBS washes (3 × 5 min) and incubation with primary antibody (mouse anti-GFP, A11120, Life technologies; 1:200 ...
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bioRxiv - Physiology 2023Quote: ... R: 5’ TGCCTTCGTGCCGTACTG 3’) and confirmed by DNA sequencing following sub-cloning into pCRTM-Blunt (Invitrogen). Further genotypes were determined by real-time PCR with specific probes designed for each gene (Transnetyx ...
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bioRxiv - Physiology 2023Quote: ... were incubated for 20 minutes with 5 µM of Di-3-ANEPPDHQ (Thermo Fisher Scientific, USA) membrane dye ...
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bioRxiv - Genetics 2023Quote: ... and the random primer 5′-GTTTCCCAGTCACGATCNNNNNNNNNNNNNNN-3′ for the RT step and recombinant Taq polymerase (Invitrogen) for the PCR step using D388 specific primers 5′-GAGAGAAGGATTAGATTGTG-3′ and 5′-CCTGTATAATAGAAGTACCA-3′ ...
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bioRxiv - Cancer Biology 2024Quote: ... plasmid containing a sgRNA targeting APC (5‘-GGCACTCAAAACGCTTTTGA-3‘) using lipofectamin-2000 (Thermo Fisher Scientific, 11668027). WT organoids were grown in WENR medium and APC truncated organoids were grown in ENR medium and are later refererred to as mCol WT and mCol APC ...
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bioRxiv - Cell Biology 2019Quote: Activation of β1 integrin was assessed by immunofluorescent staining using 9EG7 or 12G10 antibodies and AlexaFluor488-conjugated secondary antibodies (Invitrogen). Imaging was performed using a fluorescence microscope (Olympus ...
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bioRxiv - Physiology 2020Quote: β1 integrin was identified on the VSMC surface using AFM probes labeled with fibronectin (1mg/ml) (Invitrogen, Grand Island, NY). Probes were operated in force-mode and repeated to indent and retract from the cell surface and specific adhesions detected as sharp shifts in the retraction curves using automated software (NforceR ...
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bioRxiv - Developmental Biology 2021Quote: ... and maintained with alpha MEM plus 10% FBS (mesenchymal stem cell-qualified, Gibco), 25 units/mL penicillin/streptomycin and 2 mM L-glutamine at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... Both mouse cell lines were cultured in Minimum Essential Medium (MEM) Alpha (Gibco). The media used for the above cell lines were supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: ... NK92 cells (KeyGEN BioTECH, Nanjing, China) were cultured in Alpha MEM (GIBCO, USA) with 2 mM L-glutamine ...