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Citations for Thermo Fisher :
1701 - 1750 of 10000+ citations for Human integrin alpha 5 beta 3 His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2022Quote: ... human CTRP3 (Invitrogen, PA5-115061, rabbit anti-human), α-SMA-Cy3 (Sigma ...
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bioRxiv - Cancer Biology 2019Quote: ... Individual samples were then labeled with isobaric tags using commercially available Tandem Mass Tag™ 6-plex (TMTsixplex™) (Thermo Fisher Scientific, P/N 90061) or TMT11plex (TMT11plex™ ...
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bioRxiv - Cancer Biology 2019Quote: ... human recombinant Epidermal Growth Factor 1-53 (EGF 1-53, 5 ng/ml, Thermo Fisher Scientific, catalog #17005-042) and 1% penicillin/streptomycin ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... cells were isolated from human foreskin tissue and cultivated at 37 °C and 5% CO2 in Eagle’s minimal essential medium (Gibco, Thermo Fisher Scientific Inc. ...
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bioRxiv - Pathology 2020Quote: ... CHO cells were transfected with 5 ug of human cadherin-6 in pIRES-puro or mouse cadherin-6 in pCDNA3.1 via Lipofectamine 2000 (Invitrogen). After 48hrs ...
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bioRxiv - Molecular Biology 2020Quote: Human HCT116 cells (ATCC) were cultured at 37°C under 5% CO2 in McCoy’s 5A media (Thermo Fisher Scientific) with 1x penicillin/streptomycin and 10% FBS (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: Synthetic genes encoding for human merlin FERM (22-312) and radixin FERM (5-295) domains were commercially synthesized (ThermoFisher) and cloned into pETM33 using NcoI and EcoRI restriction sites ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 0.05 mg/mL bovine pituitary extract and 5 ng/mL human recombinant epidermal growth factor (Gibco, USA). Cells were maintained in a humidified incubator at 37°C with 5% CO2 level ...
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bioRxiv - Microbiology 2022Quote: Human HEK-293T adherent cells were seeded at 37°C with 5% CO2 in DMEM + GlutaMAX™ (Gibco, Germany) containing 10% heat inactivated fetal calf serum (dFCS ...
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bioRxiv - Molecular Biology 2023Quote: ... Human dispersed islets were seeded at a density of 5×104 cells/cm2 on ultra-low attachment plates (ThermoFIsher) and allowed to form spontaneous pseudoislets ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 5% heat-inactivated fetal bovine serum and activated with Dynabeads Human T-Activator CD3/CD28 (ThermoFisher Scientific) at a 1:1 bead:cell ratio according to the manufacturer’s instructions ...
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Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: ... T cells were plated at 5×104 per well and stimulated with human CD3/CD28 T-Activator DynaBeads(Gibco) at 1:8 beads:T cell ratio ...
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bioRxiv - Developmental Biology 2023Quote: WTC-mTagRFPT-LMNB1 human iPSCs were dissociated into single cells with 5 min incubation in Accutase (Life Technologies, #A1110501) at 37°C ...
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bioRxiv - Genomics 2023Quote: ... human female) cells were cultured in a 37°C 5% CO2 and 20% O2 humidified incubator with DMEM (Gibco) supplemented with 10% heat-inactivated FBS (Gibco) ...
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bioRxiv - Microbiology 2023Quote: ... were propagated in 5% of human O+ erythrocytes and 10 mL of RPMI-1640 medium containing 0.5% Albumax II (Invitrogen), 2 mM L-glutamine ...
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bioRxiv - Cell Biology 2023Quote: ... were grown in keratinocyte serum-free medium supplemented with 0.05 mg/ml bovine pituitary extract and 5 ng/ml human recombinant epidermal growth factor (Gibco). T24 human bladder cancer cells were cultured in HAM’s F12 medium supplemented with 10% heat-inactivated FBS and 1% penicillin/streptomycin/glutamine (PSG) ...
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bioRxiv - Cancer Biology 2024Quote: ... plastic flat bottom 96 well plates were coated with 5 μg/mL human fibronectin in PBS (GIBCO, 14080-055) for 1h in 37 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... human (Invitrogen). Cells were cultured in DMEM (Corning Cellgro 10-013-CV ...
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bioRxiv - Plant Biology 2019Quote: ... the full-length GAD2 was also amplified using primer set GAD2_forward2 (5’-CACCATGGTTTTGACAAAAACCGC-3’) and GAD2_reverse and cloned into pENTR/D-TOPO vector via directional cloning (Invitrogen), followed by a LR reaction recombinant into pMDC3240 ...
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bioRxiv - Immunology 2021Quote: ... RT-PCR was performed on QuantStudio™ 3 and 5 Real-Time PCR System (Thermo Fisher Scientific, USA) using KAPA SYBR®Fast qPCR Master Mix (Kapa Biosystems).
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed 3 x 5 min and incubated for 10 min with DAPI (1/2000, Invitrogen #D1306) diluted in PBT + 0.5% BSA ...
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bioRxiv - Cancer Biology 2022Quote: ... for 5 minutes followed by a wash with chilled PBS and blocked with 3% goat serum (Thermo Scientific) for 1h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... 3-5 μl of purified γ-TuRC was visualized on an SDS-PAGE with SYPRO Ruby stain (ThermoFisher) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... hBMSCs (passage 3) were expanded at 37°C and 5% CO2 in expansion medium (Dulbecco’s modified Eagle’s medium (DMEM; GIBCO) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2020Quote: ... by incubating muscles twice for 45 min in preoxygenated Ringer containing 5 μM Rhod-3 AM (Life Technologies), 0.02 % Pluronic acid (Life Technologies ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... Reverse 5′-CGA AGG TGT GAC TTC CAT G-3′) on a QuantStudio 6 Flex thermocycler (Applied Biosystems). Standard curve was established in parallel using purified SARS-CoV-2 viral RNA.
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bioRxiv - Neuroscience 2020Quote: ... brain sections from 3- to 5-month-old mice were exposed to 2.5 µM MitoSOX Red (Molecular Probes), at 37°C for 30 min14 ...
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bioRxiv - Plant Biology 2020Quote: ... The solution was sonicated for 3-5 mins using an ultrasonic cleaner (FS220, Thermo Fisher Scientific, Waltham, MA). The solution was diluted to 10 mL with 2% nitric acid ...
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bioRxiv - Cell Biology 2021Quote: ... 5 to 20 ng of RNA were amplified and labelled with the GeneChip 3’ IVT Pico Kit (Affymetrix) according to manufacturer’s protocols ...
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bioRxiv - Cell Biology 2021Quote: ... incubated for 3 to 5 min in 100 μM Lysotracker-Red DND-99 (Life Technologies, Carlsbad, CA, USA)[49] and 1 μM Höchst 33342 (Thermo Fisher Scientific Inc ...
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bioRxiv - Genomics 2020Quote: ... slides were washed 3 times for 5 min with TNT and mounted with Prolong Gold (P36930, Life Technologies).
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bioRxiv - Molecular Biology 2019Quote: ... miR-125b+A or control RNA 3’O-methyl-modified mimic using 5 μl Lipofectamine 2000 (Life Technologies) in a 500 μl reaction mixture according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... rinsed again (3 × 5 min) in 1X PBS and mounted using SlowFade™ Diamond Antifade Mountant (Invitrogen; S36963). Fluorescence images were acquired with a confocal laser scanning microscope (LSM 700 or 710 ...
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bioRxiv - Microbiology 2019Quote: ... PUUV-Gc-rev 5’-ATTCCCGGGCTTGTGCTCCTTC-3’ to PCR amplify (Phusion Flash High-Fidelity PCR Master Mix, ThermoFisher Scientific) the inserts from codon optimized PUUV GPC described in Iheozor-Ejiofor et al. ...
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bioRxiv - Plant Biology 2021Quote: ... The bands were detected by using SuperSignal pico/femto chemiluminescence kits (Thermo Scientific; #’s1859022/3 and 1859674/5).
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bioRxiv - Zoology 2020Quote: ... 2’-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-23491-52-3 (Hoechst 33342, trihydrochloride, trihydrate, Life Technologies, H3570) in water for 20 min ...
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bioRxiv - Biochemistry 2020Quote: ... 3 μg/mL gentamicin (Wisent) and 5 ng/mL recombinant mouse fibroblast growth factor basic (FGF-b, ThermoFisher). At ~90% confluence ...
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bioRxiv - Cell Biology 2021Quote: ... cells were treated with DMNB-caged cAMP (4,5-Dimethoxy-2-Nitrobenzyl Adenosine 3’,5’-Cyclicmonophosphate, Molecular Probes, D1037) for at least 30 minutes prior to imaging at a final concentration of 1 mM ...
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bioRxiv - Immunology 2020Quote: ... 5% CO2 and medium were changed every 3 days and cells were passaged with 0,05% trypsin-EDTA (Invitrogen) twice per week.
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bioRxiv - Genomics 2020Quote: ... Biotin labelled fragments which contained the 5’ and 3’ connecting region was enriched using Dynabeads M280 streptavidin (Invitrogen) and incubating at room temperature for 30 min ...
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bioRxiv - Microbiology 2022Quote: ... Vero-E6 cells and Calu-3 were treated with 5 μg/ml or 1 μg/ml puromycin (Gibco), respectively ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Sections were washed 3 times 5 minutes in PBS and mounted using ProLong Gold mouting media (Thermo Fisher) under glass cover slips.
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bioRxiv - Developmental Biology 2023Quote: 5’ - capped and 3’- polyadenylated hCas9-2xNLS mRNA was transcribed in vitro using mMessageMachine T7 (ThermoFisher, Cat# AM1344) and Poly(A ...
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bioRxiv - Neuroscience 2023Quote: ... Real-time PCR (RT-PCR) were performed on a QuantStudio 3 or QuantStudio 5 machines (Thermo Fisher Scientific). Primers/probe sets from Applied Biosystems were selected from the Thermo Fisher Scientific website (Supplementary Table 3) ...
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bioRxiv - Immunology 2023Quote: ... 5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro blue tetrazolium (NBT) substrate (Thermo Fisher Scientific, cat.: 34042) was added and the reaction was terminated after 5 min under running tap water ...
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bioRxiv - Genetics 2023Quote: ... 3-5 million cells or approximately 15 ug of DNA crosslinked with 2% PFA (Fisher Scientific F79-500) were used as input per reaction ...
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bioRxiv - Molecular Biology 2023Quote: ... An RNA linker (5’-CACGACGCUCUUCCGAUCU-3’) was ligated to the polyA RNA with T4 RNA ligase (Thermo Scientific) to capture RNAs with a 5’ monophosphate (5’P) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with DMNB-caged cAMP (4,5-Dimethoxy-2-Nitrobenzyl Adenosine 3’,5’-Cyclicmonophosphate, Molecular Probes, D1037) for at least 30 min prior to imaging at a final concentration of 1 mM ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Slides were again washed with PBS-/- 3×5 min and mounted using Prolong Gold Antifade Mountant (Thermo Scientific). Sections stained with secondary only antibodies were included to determine fluorescence threshold before image acquisition in each channel ...
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bioRxiv - Biochemistry 2022Quote: ... 2-3 µL of 5 µM protein solution was introduced directly into Q-Exactive UHMR mass spectrometer (ThermoFisher) through gold coated capillary needles that were prepared in-house30 ...