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Citations for Thermo Fisher :
1451 - 1500 of 10000+ citations for Glycerol Kinase 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Proteins were digested with trypsin (at a 1:50 trypsin to protein ratio) and resulting peptides were desalted on a C18 Hypersep column (Thermo Scientific) and dried down using a speed-vac ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were digested with trypsin (at a 1:50 trypsin to protein ratio) and resulting peptides were desalted on a C18 Hypersep column (Thermo Scientific) and dried down using a speed-vac ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were digested with trypsin (at a 1:50 trypsin to protein ratio) and resulting peptides were desalted on a C18 Hypersep column (Thermo Scientific) and dried down using a speed-vac ...
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bioRxiv - Immunology 2022Quote: ... the captured viral biotinylated peptides were detected with 1 mg/ml pNPP (p-nitro-phenyl-phosphate, Thermo Fisher Scientific, cat: 34045) diluted in Diethanolamine substrate buffer 1X (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2022Quote: Samples were suspended in 0.1% TFA and an equivalent to 1 µg of peptides was analyzed using Ultimate 3000 liquid chromatography system coupled to an Orbitrap QE HF (Thermo Fisher). An in-house packed analytical column (75 µm x 200 mm ...
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bioRxiv - Genomics 2022Quote: ... Microarrays were then washed (three times with PBS-T for 1 minute) and peptide binding was detected with isotype-specific secondary goat anti-human IgG (Fc) DyLight680 (ThermoFisher Scientific) and goat anti-human IgA (alpha chain ...
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bioRxiv - Systems Biology 2021Quote: ... lyophilized peptides were resuspended in 105 µl of equilibration buffer (1% TFA/ 80% ACN) and the peptide concentration was measured optically at 280nm (Nanodrop 2000, Thermo Scientific) and subsequently equalized using equilibration buffer ...
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bioRxiv - Immunology 2021Quote: ... Peptide and enzyme solutions were dissolved and diluted in 25 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES, Fisher Scientific). The peptide (100 μM in a total volume of 100 μL ...
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bioRxiv - Immunology 2021Quote: ... RNA-optimized Spike genes were designed based on the SARS-CoV-2 Spike protein sequence from Wuhan-Hu-1 (GenBank: MN_908947) with its original signal peptide and the DNAs were synthesized by GeneArt (Thermo Fisher Scientific). All Spike plasmids have the 2P stabilizing mutations by substituting of K986V987 [34–36] ...
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bioRxiv - Developmental Biology 2022Quote: ... Digested peptides were diluted to 60 μL with water and labeled for 1 hour at room temperature using 16plex TMTpro (Thermo Scientific) or 10plex TMT (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were reconstituted in 10 µl of H2O and reacted for 1 h at room temperature with TMT10 (Thermo Scientific, #90110) or TMTpro 18-plex (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were then pooled and dried to near completion under reduced pressure before resuspension in 1% formic acid and fractionation using a Pierce High pH Reversed Phase Peptide Fractionation Kit (ThermoFisher Scientific) with modified elution of 12 sequential fractions (10% ...
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bioRxiv - Molecular Biology 2023Quote: ... 1/10 of peptide solution was injected in a nanoRSLC-Q Exactive PLUS (RSLC Ultimate 3000) (Thermo Scientific,Waltham MA, USA). Peptides were loaded onto a µ-precolumn (Acclaim PepMap 100 C18 ...
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bioRxiv - Microbiology 2023Quote: ... 1 μg of peptide samples were loaded onto a C18 reversed□phase trap column (100 μm × 2 cm, Thermo Fisher Scientific) equilibrated with 100% A phase ...
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bioRxiv - Biochemistry 2023Quote: ... at a trypsin:protein ratio of 1:20 and peptides were purified using C18 tips (Pierce C18 Tips, 100-μl bed; Thermo Fisher Scientific). Purified peptides were diluted to 0.1 μg μl−1 and 20 μl (2 μg ...
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bioRxiv - Plant Biology 2024Quote: ... A total of 1 μg of re-suspended peptide was injected per replicate using and Easy-nLC 1200 system (LC140; Thermo Scientific) mounted with an Acclaim PepMap 100 C18 trap column (Cat# 164750 ...
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bioRxiv - Zoology 2021Quote: ... We standardized C-peptide measurements by dividing C-peptide concentrations by creatinine concentrations (measured via enzyme immunoassays with an auto analyzer: Gallery, Thermo Fisher Scientific, Waltham, MA, USA). C-peptide levels are therefore reported as pg/mg of creatinine ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were reconstituted in 50 mM TEAB and their concentrations were determined using the Pierce™ quantitative fluorometric peptide assay (Thermo Fisher Scientific, Waltham, MA). Twelve micrograms of peptides were labelled with TMT labels (6-plex ...
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bioRxiv - Molecular Biology 2023Quote: ... Ten labeled samples in each of TMT 10-plex sets were pooled together and 80 µg of labeled tryptic peptides were then fractionated using a Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (Thermo-Fisher Scientific, San Jose, CA) with a 9-step isocratic elution ...
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bioRxiv - Cancer Biology 2021Quote: ... in blocking buffer were incubated overnight at 4°C followed by washing and incubation with Alexa Fluor conjugated secondary antibodies (Invitrogen, 1:1000 dilution) at room temperature for 1-2 hour in the dark ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and incubated 1 hour at RT with secondary antibodies diluted in blocking solution as follows: anti-guinea pig Dylight 488 (Thermo Scientific, SA5-10094) (0.5 μg/mL ...
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bioRxiv - Neuroscience 2021Quote: ... Staining media was prepared in blocking buffer using alpha-Bungarotoxin (BTX) conjugated with Alexa Fluor 555 (ThermoFisher, B35451, 1:200 dilution in PBS). Additionally ...
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bioRxiv - Molecular Biology 2021Quote: ... blocked with 600 μL blocking buffer (33% sonication buffer, 67% 2x hybridization buffer, 500 ng/μL yeast RNA [Thermo Fisher Scientific], 1% BSA) at room temp for 1 hr ...
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bioRxiv - Neuroscience 2019Quote: ... slices were washed in PBS and then incubated in PBS blocking buffer containing goat anti-rabbit 2o antibody conjugated with an Alexaflour 594 (Life Technologies, 1:500) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... slices were washed in PBS and then incubated in PBS blocking buffer containing goat anti-rabbit 2o antibody conjugated with an Alexafluor 594 (Life Technologies, 1:500) for 1 hour at room temperature.
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bioRxiv - Physiology 2020Quote: ... Slides were subsequently exposed to a 1:5000 dilution of the primary antibody in blocking solution (Alexa488-conjugated rabbit anti-GFP; Life Technologies, Waltham, MA) for 3 hours at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... in blocking buffer were incubated overnight at 4°C followed by washing and incubation with Alexa Fluor conjugated secondary antibodies (Invitrogen, 1:1000 dilution) at room temperature for 1-2 hour in the dark ...
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bioRxiv - Bioengineering 2020Quote: ... Slides were blocked in serum-based blocking buffer and incubated with primary antibody against MMP12 (MA5-32011, Invitrogen, 1:50, 20 μg/mL). Slides were washed in PBS (3×5 minutes) ...
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bioRxiv - Cancer Biology 2019Quote: ... the sections were incubated overnight at room temperature with the indicated primary antibody prepared in blocking/permeabilization solution: mouse monoclonal antibody against HK-2 (1:100; Thermo Fisher, MA5-15679) or mouse monoclonal antibody against GLUT-3 (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... a secondary antibody was applied to the sections for 2 hours (AlexaFluor goat anti-mouse 594 diluted 1:400 in blocking buffer; Life Technologies, Carlsbad, CA). Sections were again washed in PBS over 30 minutes prior to the application of mounting media and coverslips.
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bioRxiv - Developmental Biology 2022Quote: ... The samples were washed (4 times 30 min, PBST) and then incubated with the secondary antibody (Alexa fluor 647, 1: 1200 ThermoFisher in blocking solution) overnight at 4°C with gentle rotation ...
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bioRxiv - Cell Biology 2022Quote: ... Then samples were incubated for 30 min in blocking buffer containing 2% (w/v) BSA and then incubated with Alexa Fluor 488 phalloidin (1:400 dilution, Thermo Fisher Scientific, A12379) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were then incubated overnight (4°C) in the same blocking solution containing the primary rabbit anti-PV antibody (1:1000; Thermo Scientific, PA1-933) and mouse anti-GFP antibody (1:500 ...
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bioRxiv - Genetics 2019Quote: ... primary antibodies in blocking buffer containing 5% milk followed by the secondary antibodies of goat anti-mouse IgG (H+L) HRP (Life Technologies, 1:10000) or goat anti-rabbit IgG (H+L ...
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bioRxiv - Biochemistry 2022Quote: ... coverslips were washed 3 x 10 min in PBS containing 0.1% Triton X-100 at room temperature and then incubated for 1 hr at room temperature with secondary antibodies diluted in blocking buffer: AlexaFluor594 goat anti-mouse IgG (Molecular Probes, ThermoFisher Scientific, AB_2534091) and AlexaFluor488 Streptavidin (Molecular Probes ...
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bioRxiv - Cell Biology 2022Quote: ... and lung cryosections after blocking were stained with a 1:200 dilution of Alexa Fluor 488 Phalloidin (Thermo Fisher Scientific, Rockford, IL, USA) for 1 h ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The cells were washed 5x with blocking solution and a 1:500 dilution of secondary antibodies (Invitrogen Goat anti-rabbit IgG (H+L) Superclonal™/Invitrogen Alexa Fluor™ 594 goat anti-mouse IgG2a (γ2a ...
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bioRxiv - Molecular Biology 2023Quote: ... The following secondary antibodies were added overnight at 4°C in blocking buffer: Alexa Fluor™ 647 donkey anti-rabbit IgG (1:1000, Invitrogen, #A-32795), Alexa Fluor™ 488 donkey anti-mouse IgG (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were washed for 1 hour in 1x PBS twice at room temperature and then incubated in a solution containing 1% Triton X-100 (93443, MilliporeSigma) in StartingBlock (PBS) Blocking Buffer (37538, ThermoFisher Scientific, Waltham, MA) overnight at room temperature to permeabilize and block the tissue ...
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bioRxiv - Neuroscience 2022Quote: ... the fixed tissue cultures were incubated for 1 h at room temperature in blocking solution consisting of 10 % (v/v) normal goat serum (NGS; Fisher Scientific, Cat# NC9270494) and 0.5 % (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... for 2 hours at RT and then in the blocking buffer containing monoclonal anti-GFP mouse antibody (GF28R, 1:3000, Thermo Fisher, #MA5-15256) and biotin-conjugated anti-ALFA nanobody (1:1000 ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were washed with blocking solution and incubated with species-specific Alexa Fluor 488 and 555 conjugated secondary Abs (1:200) (Invitrogen, Carlsbad, CA, USA) and DR™ nuclear stain (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... MSD GOLD 96-well small-spot streptavidin-coated microtiter plates (Meso Scale Discovery, Rockville, MD) were treated with a blocking step of 1% casein-based PBS (Thermo Scientific, Waltham, MA) for approximately 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated overnight at 4°C with primary antibodies diluted in blocking solution: rat anti-Tbr2 (1:500, Invitrogen 14-4875-80), rabbit anti-tyrosine hydroxylase (TH ...
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bioRxiv - Neuroscience 2023Quote: ... for 5 min each time and subsequently incubated with blocking buffer (5% goat serum [R&D Systems, Minneapolis, MN, Catalog no. S13110], 1% bovine serum albumin [Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then washed 2x with Blocking Buffer and subsequently incubated with Alexa Fluor 633 goat anti-mouse IgG (Invitrogen, A21050, 1:400), Alexa Fluor 555 goat anti-rabbit IgG (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... they were incubated with the secondary antibodies diluted in blocking solution: Alexa Fluor 488-conjugated goat anti-chicken IgY (A-11039, Invitrogen, dilution 1:1000) and Alexa Fluor 594-conjugated goat anti-mouse IgG (A-11020 ...
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bioRxiv - Microbiology 2024Quote: ... diluted each to 1 µg/mL in blocking buffer (PBS containing 2% BSA [Sigma-Aldrich], 1% bovine gelatin [Sigma-Aldrich], and 0.05% Tween 20 [Thermo Fisher Scientific]) was added and plates incubated for 2 h at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were again re-blocked with 5% NGS for 2 hr (second serum blocking) and subsequently stained with rat anti-GFAP (1:500; Invitrogen Cat. #13-0300), and rabbit anti-Megf10 (1 ...
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bioRxiv - Microbiology 2021Quote: ... Probes consisting of oligodeoxyribonucleotides (see Supplementary Table 2) were 5′-end labeled using T4 polynucleotide kinase (ThermoFisher Scientific) with 25 μCi of [γ-32P]dATP ...