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Citations for Thermo Fisher :
1701 - 1750 of 10000+ citations for Glycerol Kinase 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Resulting peptides were dried down using a SpeedVac concentrator (Thermo Scientific).
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bioRxiv - Immunology 2023Quote: ... Peptides were separated on an EASY-nLC 1200 System (Thermo Fisher) using 20 cm long fused silica capillary columns (100 μm ID ...
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bioRxiv - Genetics 2023Quote: ... then fully desalted using Pierce peptide desalting spin columns (Thermo Scientific) per manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The peptides were separated by an Easy-nLC system (Thermo Scientific) equipped with a reverse-phase trapping column Acclaim PepMapTM 100 (C18 ...
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bioRxiv - Biochemistry 2024Quote: ... peptides were loaded onto a 2cm C18 trap column (ThermoFisher 164705), connected in-line to a 15 cm C18 reverse-phase analytical column (Thermo EasySpray ES804A ...
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bioRxiv - Biochemistry 2024Quote: ... The labeled peptides were analyzed in Orbitrap Fusion Mass Spectrometer (ThermoFisher).
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bioRxiv - Cell Biology 2024Quote: ... and quantified using the Pierce Quantitative Fluorometric Peptide Assay (ThermoFisher, #23290).
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bioRxiv - Cell Biology 2024Quote: Peptide separations were carried out on a nanoHPLC Ultimate3000 (Thermo Scientific) using a nano column EASY-Spray ES901 (15 cm × 50 μm ID ...
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Increased dosage of wild-type KRAS protein drives KRAS-mutant lung tumorigenesis and drug resistancebioRxiv - Cancer Biology 2024Quote: Peptides were loaded onto a 2cm C18 trap column (ThermoFisher 164946), connected in-line to a 15cm C18 reverse-phase analytical column (Thermo EasySpray ES904 ...
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bioRxiv - Molecular Biology 2024Quote: Peptide analysis occurred on Orbitrap Eclipse Tribrid mass spectrometers (Thermo Scientific), connected to a Dionex UltiMate 3000 RSLCnano system (Thermo Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were labelled with TMT10plex(80) Isobaric Label Reagent (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Secondary antibody probing was for 1 hour in the dark with the following antibodies at 1:1000 in blocking buffer: goat anti-rabbit Alexa Fluor 594 (Life Technologies, Carlsbad CA, A11037) and goat anti-mouse Alexa Fluor 488 (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... The secondary Alexa Fluor 546 donkey anti-rabbit IgG and Alexa Fluor 488 donkey anti-mouse IgG (Invitrogen, 1:500, diluted in blocking solution) antibodies were added together with 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cell Biology 2021Quote: ... permeabilized with 0.1% Triton X-100 in PBS for 10 min and blocked with 2% BSA solution in PBS for 1 h followed by overnight incubation at 4°C with primary antibodies diluted in 2% BSA blocking solution: mouse monoclonal [1F7G5] anti-CKS2 (1:100 dilution; Thermo Fisher Scientific; 37-0300), rabbit polyclonal anti-P63 (p63α ...
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bioRxiv - Bioengineering 2020Quote: ... and secondary antibodies prepared in blocking solution were applied for 2 hours at room temperature: donkey anti-mouse 568 (Alexa Fluor® 568, 1:500, Thermo Scientific Cat# A10037) and donkey anti-rabbit 647 (Alexa Fluor® 647 ...
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bioRxiv - Cell Biology 2021Quote: ... were performed overnight at 4°C in 1:100 dilutions in blocking solution and later secondary antibody incubation with Alexa Fluor 594 (antimouse, ref. R37121; Thermo Fisher Scientific, United States) and Alexa Fluor 488 (antirabbit ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated for 2 h in blocking/permeabilization buffer with goat anti-mouse and/or goat anti-rabbit AlexaFluor 568 (Invitrogen, Carlsbad, CA; 1:500) secondary antibodies ...
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bioRxiv - Biochemistry 2020Quote: ... The measurement was performed in 3 steps - 1st step blocking solution (50% PBS BSA 1/mg/mL, 50% Superblock (Thermo Scientific - Cat No: 37515)) ...
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bioRxiv - Systems Biology 2019Quote: ... the membrane was probed overnight at 4°C with the Rabbit anti-TAP polyclonal primary antibody (Thermo Fisher, 1:5000 dilution in blocking solution), which recognizes an epitope at the region between the TEV-cleavage site and His6 ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 2 h in blocking/permeabilization buffer with goat anti-rabbit AlexaFluor 488 and goat anti-chicken AlexaFluor 568 (Invitrogen, Carlsbad, CA; 1:500). After washing ...
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bioRxiv - Physiology 2021Quote: ... After primary incubations the RBCs were washed three times in PBS and incubated for three hours on a rotator at room temperature in blocking buffer containing secondary antibodies: 1:500 goat anti-mouse (AlexaFlour 568; Thermo Fisher Life Technologies A-11031), 1:500 goat anti-rabbit (AlexaFlour 488 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated overnight at 4°C with the following antibody dilutions in blocking solution: anti-Iba1 (1:1000; Thermo Fisher Scientific, Waltham, MA), anti-P2RY12 (1:1000 ...
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bioRxiv - Biochemistry 2022Quote: ... coverslips were washed 3 x 10 min in PBS containing 0.1% Triton X-100 at room temperature and then incubated for 1 hr at room temperature with secondary antibodies diluted in blocking buffer: AlexaFluor594 goat anti-mouse IgG (Molecular Probes, ThermoFisher Scientific, AB_2534091) and AlexaFluor488 Streptavidin (Molecular Probes ...
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bioRxiv - Bioengineering 2022Quote: ... cells were washed with 5 mL of PBS and incubated with a blocking buffer comprised of 1% bovine serum albumin (BSA, Fisher Scientific cat. no. BP9703) and 10% goat serum (Sigma Aldrich cat ...
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bioRxiv - Cell Biology 2022Quote: ... then room temperature blocking solution (3% BSA in D-PBS) containing secondary antibody (IgG2b Cross-Adsorbed Goat anti-Mouse, Alexa Fluor™ 555 [Invitrogen, 10412832], 1:2000) was added using Dragonfly Discovery liquid dispenser and incubated for 1 h at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... They were then treated with secondary antibody solution (1 : 200 of Alexa Fluor 488 goat anti-rabbit IgG; Molecular Probes, in the blocking buffer) for 2 hours at 37 °C and washed three times ...
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LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: ... and then incubated in the same blocking solution containing secondary antibody (Donkey anti-rabbit Alexa fluorophore 647, 1:500 Thermo Fisher Scientific, Waltham, MA) for 1 hour at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then washed 3 times with PBS and then blocked for 30 minutes at room temperature with antibody blocking buffer (150 mM NaCl, 50 mM Tris-Base, 1% BSA, 100 mM L-lysine 50% normal goat serum (Thermo Fisher, Cat# 01-6201), 0.2% Triton (Roche ...
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bioRxiv - Neuroscience 2023Quote: Slices were stained for green fluorescent protein (GFP) with a rabbit monoclonal anti-GFP antibody (G10362, Molecular Probes, 1:1000 dilution in blocking buffer) and for tyrosine hydroxylase (TH ...
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bioRxiv - Neuroscience 2024Quote: ... Slice were then incubated overnight at 4°C in blocking solution containing a rabbit anti-Parvalbumin (PV) antibody (1:400, cat # PA1933, Thermo Fisher Scientific, Waltham, MA). Slices were then washed and incubated blocking solution containing a secondary antibody donkey anti-rabbit Alexa568 diluted to 1:1000 (Abcam ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for 2 h in blocking/permeabilization buffer with goat anti-rabbit AlexaFluor 488 and goat anti-chicken AlexaFluor 568 (Invitrogen, Carlsbad, CA; 1:500). After washing ...
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bioRxiv - Cell Biology 2019Quote: ... a total of 1 ug of peptides was injected into a Dionex Ultimate 3000 NanoRSLC instrument with a 15-cm Acclaim PEPMAPC18 (Thermo Fisher Scientific #164534) reverse phase column ...
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bioRxiv - Microbiology 2021Quote: ... PM-WCPV-S) or N peptide pool (Meltenyi, 130-126-698) in the presence of 1 µg/mL anti-CD28 (Invitrogen, 14-0281-86) for co-stimulation for 6 hours ...
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bioRxiv - Cancer Biology 2022Quote: The peptide samples prepared for building Nomo-1 sufaceome custom database were loaded on to the an EASY-Spray nanocolumn (Thermo Fisher Scientific, ES900) installed on Dionex Ultimate 3000 NanoRSLC instrument coupled with Q-Exactive Plus mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... polyclonal antibody (1:100) was raised against a synthetic peptide sequence and prepared for our laboratory commercially by ResGen (Invitrogen, Carlsbad, CA, USA), as described61 ...
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bioRxiv - Physiology 2023Quote: Fractions were resuspended in 0.1% (v/v) formic acid (solvent A) and the peptides were separated and analyzed using an EASY-nLC system (Thermo Fisher Scientific, USA) with a two-column system setup coupled to a Q-Exactive HF mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... 20 % PEG400) and 5’ end was phosphorylated by treating RNA with T4 Polynucleotide Kinase (1x PNK buffer (Thermo Scientific), 1mM ATP) ...
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bioRxiv - Biochemistry 2020Quote: ... in which case the phosphorylation of the oligos was performed by using the T4 Polynucleotide Kinase from Thermo Fisher, according to manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and 5 pmol was 5’-end labeled with 20 μCi of Ψ-P32 ATP using Polynucleotide Kinase (Thermo Fisher) and subsequently purified using an Illustra MicroSpin G-50 column (GE Healthcare) ...
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bioRxiv - Biophysics 2019Quote: ... at a concentration of ∼150 µM and then 2 mM of ATP and 0.42 μg of PLK3 kinase ( Invitrogen) per 500 μg of protein were added ...
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bioRxiv - Microbiology 2020Quote: ... beads were magnetized and resuspended in 100 μl PNK mix [10 units of T4 polynucleotide kinase (EK0032, ThermoFisher Scientific), 10 μCi γ-32P-ATP in PNK buffer] ...
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bioRxiv - Biochemistry 2020Quote: Poly-A RNA 27mer and 40mer were chemically synthesized (Biomers) and 32P-labelled with T4 polynucleotide kinase (Thermo Fisher) or ScriptCap m7G Capping System (CellScript) ...
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bioRxiv - Plant Biology 2022Quote: ... cold ATP was used in the kinase assays at 200 μM and detection was using ProQ diamond stain (Invitrogen).
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bioRxiv - Neuroscience 2022Quote: ... was in vitro phosphorylated overnight at 30°C by incubation with purified PLK3 kinase (4.2 μg/ml, #PV3812, ThermoFisher) in a buffer consisting of 20 mM Hepes pH 7.4 ...
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bioRxiv - Molecular Biology 2023Quote: ... The remaining beads were resuspended in 20 μl of the radiolabelling mixture (10U of T4 polynucleotides Kinase (Thermo Scientific), reaction buffer A (Thermo Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... and rho kinase inhibitors such as a CEPT cocktail consisting of 5 µM Emricasan (50-136-5235, Fisher Scientific), 50 nM Chroman 1 (7163 ...
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bioRxiv - Developmental Biology 2023Quote: ... Rho associated kinase inhibitor (10μM, Y-27632) and Geltrex (0.5 μl/cm2 well surface area, Thermo Fisher Scientific, A1413302) were added to media during replating ...
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bioRxiv - Genomics 2020Quote: ... 25 μL ProteinaseK (25mg/ml in TE with 50% glycerol) and 25 μL 10% SDS (Thermo Fisher, 15553035) were added and the sample was incubated at 65°C o/n ...
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bioRxiv - Microbiology 2022Quote: ... 50 mM KCl, 100 mM Tris-HCl [pH 7.4], 40% glycerol, 0.4 U/μl of Superase·IN [Life Technologies]) and incubate at room temperature for 10 min ...
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bioRxiv - Biophysics 2019Quote: ... 10% glycerol and 0.5% bromophenol blue) and loaded without heating on a 4-12% acrylamide NuPAGE gel (Invitrogen). Proteins were separated through electrophoresis in 50 mM MES ...