Labshake search
Citations for Thermo Fisher :
1451 - 1500 of 4555 citations for Dnp pro leu gly cys me his ala D arg nh2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... which were pre-loaded with 10 μL of Hi-Di™ formamide (ThermoFisher, Waltham, USA) and GeneScan™ -400HD ROX™ Size standard (ThermoFisher ...
-
bioRxiv - Microbiology 2024Quote: ... and passed through a 3 mL His-Pur Ni-NTA column (Thermo Scientific, catalog # 88226) according to manufacturer instruction ...
-
bioRxiv - Immunology 2024Quote: ... Blot was then incubated with 1:1000 dilution anti-his conjugated to HRP (Thermo Scientific) in 5% milk in 1xTBST (1 h ...
-
bioRxiv - Microbiology 2024Quote: ... and the other was treated with 20 U of recombinant Escherichia coli RNase HI (Invitrogen) overnight at 37 °C ...
-
bioRxiv - Immunology 2024Quote: ... Amplified PCR fragments were inserted into the metallothionein promoter driven pMT-V5-His vector (Invitrogen). The generated vector was co-transfected with a pCoBlast blasticidin resistance vector with a ratio of 19:1 and cells were selected in blasticidin containing medium according to manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... The PCR product was mixed with Hi-DiTM mix (Thermo Fisher Applied BiosystemsTM, Carlsbad, USA) with GeneScantm LIZ dye Size standardtm (Thermo Fisher Applied BiosystemsTM ...
-
bioRxiv - Molecular Biology 2024Quote: ... in HepG2 medium (Advanced MEM minus L-Glutamine [Gibco] + 10% [v/v] HI-FBS [Gibco] + 1% [v/v] penicillin-streptomycin + 2 mM Glutamax [Gibco] and 0.1% [v/v] amphotericin B) ...
-
bioRxiv - Microbiology 2024Quote: ... The METTL3 and METTL14 sequences were constructed into the pAC5.1b/V5/His expression vector (Invitrogen). The primers used to construct vectors are shown in Table S4 ...
-
bioRxiv - Neuroscience 2024Quote: ... PCR fragments of human αSyn were inserted into pTrc-His-TOPO vector (Thermo Fisher Scientific). His-tagged αSyn was purified by cOmplete™ His-Tag Purification Column (Roche) ...
-
bioRxiv - Molecular Biology 2024Quote: ... using the Ion PI™ Hi-Q™ Sequencing 200 Kit (Thermo Fisher Scientific, USA) according to standard protocols.
-
bioRxiv - Plant Biology 2024Quote: 12xHis-LIFERING was purified using Dynabeads™ His-Tag Isolation and Pulldown Beads (Thermo Scientific). 230 µl ubiquitination reaction mixture was added to 15 µl beads equilibrated and resuspended in 230 µl 2X B/W buffer (100 mM sodium phosphate pH 8 ...
-
bioRxiv - Molecular Biology 2024Quote: ... immunoblot detection was performed using Anti-His (C-term)-HRP Antibody (Invitrogen, Carlsbad, CA, USA). Bound antibodies were detected using the Amersham ECL Western Blotting Detection Reagent (GE Healthcare ...
-
bioRxiv - Immunology 2024Quote: ... followed by removal of remaining His-tagged Fabs with HisPur Ni-NTA resin (Thermo Scientific). The thrombin-protease was removed via Streptavidin Sepharose High Performance affinity resin (Cytiva) ...
-
bioRxiv - Microbiology 2020Quote: ... Extracted samples were separated in a NuPAGE 10% Bis-Tris gel in NuPAGE MES SDS Running (ThermoFisher Scientific). LPS in gels were stained with ProteoSilver Silver Stain Kit (Sigma Aldrich).
-
bioRxiv - Molecular Biology 2020Quote: ... and 30 µg loaded on a 4-12% NuPAGE Bis-tris gels in MES buffer (Novex Life Technologies) to determine eGFP and β-catenin levels ...
-
bioRxiv - Microbiology 2022Quote: ... and subjected to detergent exchange with 0.1 M MES buffer pH 6.5 complemented with 0.1% lauryldimethylamine-oxide (LDAO) detergent (Affymetrix, UK) and 25 mM NaCl ...
-
bioRxiv - Synthetic Biology 2022Quote: ... at 180 V for 30 min in 1× MES buffer (Novex) using a SeeBlue Plus2 protein standard (Invitrogen) with Coomasie Brilliant Blue R-250 (Merk ...
-
bioRxiv - Genetics 2021Quote: ... Gels were run at 120V for 2-3 hours in Bolt MES SDS Running Buffer (Thermo Fisher Scientific) prior to protein transfer to Amersham Protran nitrocellulose blotting membrane (GE Healthcare ...
-
bioRxiv - Cancer Biology 2021Quote: ... and samples were run on a NuPAGE 4-12% Bis-Tris Gel in MES SDS Running Buffer (Invitrogen). For FCG-Cas9 and FCG-Cas9 Nf1/p53 CRISPR samples ...
-
bioRxiv - Cancer Biology 2021Quote: ... and samples were run on a NuPAGE 4-12% Bis-Tris Gel in MES SDS Running Buffer (Invitrogen). For wildtype mouse astrocyte and human GBM samples ...
-
MET functions in tumour progression and therapy resistance are repressed by intronic polyadenylationbioRxiv - Molecular Biology 2023Quote: ... Samples were resolved in NuPAGE® 4-12% Bis-Tris gels using MES buffer (Invitrogen, Life Technologies, # NP0321) and transferred to a 0.45 µM nitrocellulose membrane (GE Healthcare) ...
-
MET functions in tumour progression and therapy resistance are repressed by intronic polyadenylationbioRxiv - Molecular Biology 2023Quote: ... Samples were resolved in NuPAGE® 4-12% Bis-Tris gels using MES buffer (Invitrogen, Life Technologies, # NP0321) and transferred to a 0.45 µM nitrocellulose membrane (GE Healthcare) ...
-
bioRxiv - Cell Biology 2023Quote: ... Gels were run (200V, 160 mA, 20-25 min) in 1X Bolt™ MES SDS Running Buffer (Invitrogen) and the separated proteins were then transferred onto nitrocellulose membranes (10 V ...
-
bioRxiv - Cancer Biology 2023Quote: ... alongside molecular weight protein standards (New England Biosciences) and subject to SDS-PAGE in MES running buffer (Thermofisher). Proteins were transferred onto PVDF membranes using a semi-dry transfer system (Bio-Rad ...
-
bioRxiv - Genomics 2024Quote: ... for 1 hour at 120 V in 1x NuPAGE™ MES SDS running buffer (Thermo Fisher Scientific, #NP0002). Protein bands were transferred onto a PVDF membrane (Invitrogen ...
-
bioRxiv - Biochemistry 2024Quote: ... rosetta cells) were added to wells and gels were run in Novex NuPAGE MES buffer (Invitrogen Cat# NP000202). Gels were transferred to 0.45 µm nitrocellulose (Bio-Rad ...
-
bioRxiv - Neuroscience 2024Quote: ... Proteins samples were loaded and gel migration was performed with NuPAGE™ MES SDS Running Buffer (Invitrogen™) for 45 minutes at 200mV ...
-
bioRxiv - Synthetic Biology 2024Quote: ... and separated by electrophoresis at 120 V for 2 h using nuPAGE MES SDS running buffer (Invitrogen, NP0002). The gels were transferred onto 0.2 µm PVDF membranes (Bio-Rad ...
-
bioRxiv - Bioengineering 2024Quote: ... Then 100 µL lysis buffer (10 mM MES, 150 mM NaCl, pH 6.0, B-PER II (Thermo Fisher), 50 U mL-1 Benzonase ...
-
bioRxiv - Cell Biology 2024Quote: SDS-PAGE was performed using NuPAGE 4-12% Bis-Tris precast gels using MOPS or MES buffer (ThermoFisher). a 4x LDS buffer containing 5% β-mercaptoethanol was added to samples in a 1:3 ratio ...
-
bioRxiv - Plant Biology 2022Quote: The GL2 cDNA was cloned into pENTR/D-TOPO using the pENTR/D-TOPO Cloning Kit (Invitrogen). Deletion and missense mutations gl2ΔST ...
-
bioRxiv - Plant Biology 2024Quote: ... were integrated into the pENTRTM/D-TOPOTM vector using the pENTRTM/D-TOPOTM cloning kit (Thermo Fisher), and subsequently transferred into the pGWB435 Gateway-compatible destination vector (Nakagawa et al. ...
-
bioRxiv - Developmental Biology 2021Quote: ... 8 dpf zebrafish were treated with 8 μM YO-PRO-1 dye (Y3603, Molecular Probes, OR, USA) dissolved in 1X Holtfreter’s medium for 1 hour at 28.5°C ...
-
bioRxiv - Genomics 2020Quote: LC-MS/MS runs with the Orbitrap Velos Pro MS (Thermo Fisher Scientific Inc, Waltham, MA USA) were done as described by Lerch and coworkers (54).
-
bioRxiv - Biochemistry 2021Quote: ... while the other was stained for glycoproteins using the Pro-Q Emerald 300 glycoprotein staining kit (Invitrogen) following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... colonies were tested for an L-proline aminopeptidase activity with PRO Disk (ThermoFisher Scientific, Waltham, MA, USA), to screen for C ...
-
bioRxiv - Microbiology 2021Quote: ... coupled to a Orbitrap Lumos™ Mass Spectrometer equipped with a FAIMS Pro interface (Thermo Fisher Scientific). 125-minute gradients were run for each sample altering the buffer composition from 3% Buffer B to 28% B over 95 minutes ...
-
bioRxiv - Plant Biology 2021Quote: The CSLD2KO and CSLD6KO vectors were constructed using Gateway Multisite Pro cloning (Invitrogen, Grand Island, NY, USA) as described previously (Roberts et al ...
-
Cryptococcus neoformans capsule regrowth experiments reveal dynamics of enlargement and architecturebioRxiv - Microbiology 2021Quote: ... Postincubation cells were washed once in PBS and imaged with Pro-Long Gold mounting solution (Molecular Probes). FITC channel excitation/emission was 498 and 516 nm respectively and TRITC excitation/emission was 540 and 580 nm respectively ...
-
bioRxiv - Systems Biology 2020Quote: ... Samples were cooled to room temperature before TMT labeling (TMT11 plex kit & TMT-Pro 16plex, ThermoFisher, Germany). Single cells and carrier cells sorted into the plate were labeled with 1 μl of 22mM TMT label for 1 hour at room temperature ...
-
bioRxiv - Cell Biology 2021Quote: ... The permeabilization efficiency was determined by using the non-membrane-permeable dye TO-PRO-3 iodide (ThermoFisher) (Figure 7A) ...
-
bioRxiv - Microbiology 2022Quote: ... The LPS was visualized using the Pro-Q Emerald 300 Lipopolysaccharide Gel Stain Kit (Molecular Probes, ThermoScientific) following the manufacturer’s instructions.
-
bioRxiv - Microbiology 2022Quote: ... Viral RNA was first extracted using PureLink™ Pro 96 Viral RNA/DNA Purification Kit (Thermo Fisher). Reverse transcription was carried out using Maxima H Minus First Strand cDNA Synthesis Kit (Thermo Fisher ...
-
bioRxiv - Microbiology 2022Quote: ... pyroptotic and necroptotic macrophages were revealed by staining with by 1 μM YO-PRO-1 (ThermoFisher Scientific) (Darzynkiewicz et al. ...
-
bioRxiv - Neuroscience 2022Quote: ... with 0.5 mg of Tandem Mass Tag Pro (TMTpro) reagent (16-plex kit, manufactures instructions Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2022Quote: ... with the gels subsequently stained using Pro-Q ® Diamond Phosphoprotein Gel Stain following manufacturer’s protocol (Invitrogen) and scanned again ...
-
bioRxiv - Plant Biology 2022Quote: ... Protein phosphorylation was examined by staining with Pro-Q Diamond phosphoprotein gel stain (Thermo-Fisher Scientific, Germany) following manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... for imaging and quantification using the CellInsight CX7 LED Pro High-Content Screening Platform (ThermoFisher Scientific HCSDCX7LEDPRO).
-
bioRxiv - Cell Biology 2024Quote: ... coupled to an Orbitrap Exploris 480 mass spectrometer equipped with a FAIMS Pro device (Thermo Fisher Scientific). 1 µg of each sample was injected into the analytical C18 column (75 µm inner diameter × 50 cm ...
-
bioRxiv - Immunology 2024Quote: ... Relative quantification of genes of interest was performed by qPCR analysis using QuantStudio Pro 7 system (ThermoFisher) and SYBR Green master mix (ThermoFisher) ...