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Citations for Thermo Fisher :
1701 - 1750 of 4555 citations for Dnp pro leu gly cys me his ala D arg nh2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: Plasmids expressing GST- and His-tagged proteins were transformed into BL21(DE3) competent cells (Thermo Scientific, EC0114) and grown in LB media at 37°C ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with 2 ug/ml of mouse anti-His-tag antibody (Invitrogen cat ...
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bioRxiv - Biophysics 2021Quote: ... reaction with the PCR product and an XhoI/HindIII-digested pBAD/His-B vector (Thermo Fisher Scientific). Using pBAD-GCaMP6s as the template ...
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bioRxiv - Biochemistry 2020Quote: ... 6x-His-tagged constructs of human and murine SNED1 cloned into pCDNA5/FRT (Thermo Fisher, Waltham, MA) between the FseI and AscI sites were used to transiently transfect 293T cells to validate the anti-SNED1 antibody generated in this study (Supplementary Figure S1) ...
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bioRxiv - Cell Biology 2022Quote: ... 250 µl of the clarified lysate was mixed with 150 µl HIS-Tag isolation dynabeads (Invitrogen 10103D) and 750 µl US buffer for 2 h ...
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bioRxiv - Immunology 2022Quote: ... The His-tagged proteins were purified with the HisPur Ni-NTA Resin (Thermo Fisher Scientific cat.# 88221). After washing by wash buffer (25 mM Imidazole ...
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bioRxiv - Molecular Biology 2023Quote: Drosophila S2 cells were cultured at 23°C in Schneider’s media with 10% Gibco HI-FBS (Thermofisher) and 1% Gibco Antibiotic-Antimycotic (Thermofisher) ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant AtCSD1 protein containing a 6xHis-tag was purified using Dynabeads His-Tag (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Eluted proteins were then incubated with Dynabeads His-Tag Isolation and Pulldown (Thermo Fisher Scientific, Waltham, Massachusetts) for 20 minutes and then washed 5 x 1 minute in His-tag Isolation Buffer (100 mM Na-phosphate ...
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bioRxiv - Microbiology 2024Quote: ... Cells were grown in Ham’s F-12K medium (ATCC) with HI-FBS and 1x Antibiotic-Antimycotic (Gibco) at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... The entire pENTR effector library was then transferred into the pIB/V5-His vector (Invitrogen; Table S4) using LR Clonase II (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Plates were coated overnight at 4°C with 100 ng of 6x His tag monoclonal antibodies (Invitrogen) in PBS and washed the next day ...
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bioRxiv - Biochemistry 2023Quote: ... and proteins were visualized using Ponceau stain and Western blot with monoclonal anti-His (Invitrogen MA1-21315), and polyclonal anti-GST (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Filtered supernatants containing His-tagged proteins were passed slowly through HisPur Ni-NTA Resin (Thermo Fisher 88221) beads in columns ...
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bioRxiv - Biochemistry 2023Quote: ... and co-expressed with MEI41-127 that was cloned as a His-tag fusion into pProEXHTb (Invitrogen). Supernatants were applied to Strep-Tactin XT resin (IBA ...
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bioRxiv - Developmental Biology 2023Quote: ... Su(H) coding sequence was cloned from GH10914 (DGRC Stock 5973) into pAc5.1/V5-His A (Invitrogen) using the following primers ...
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bioRxiv - Biochemistry 2023Quote: ... The His tag was digested during 14h at 4°C with histidine-tagged 3C proteases (Thermo Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2023Quote: ... the crude DEL sample was loaded in Novex™ Hi-Density TBE Sample Buffer (5X, Invitrogen LC6678), and electrophoresis was run at 12 W constant power ...
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bioRxiv - Cancer Biology 2023Quote: ... His-tagged FH(44-511) was purified from the lysate using HisPurTM Cobalt Spin Columns (Thermo Fisher). Columns were washed using with buffer A (50 mM sodium phosphate pH 7.4 ...
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bioRxiv - Immunology 2023Quote: ... using an Ion PI Hi-Q Sequencing 200 kit and Ion PI Chip v3 (Thermo Fisher Scientific). The FASTQ files were generated using AmpliSeqRNA plug-in v5.2.0.3 in the Torrent Suite software (v5.2.2 ...
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bioRxiv - Biochemistry 2023Quote: His-mATGL and its point mutants were recombinantly expressed in Expi293FTM cells (Thermo Fisher Scientific, Waltham, USA). The cells were cultivated in Expi Expression Medium (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: All RBD antigens were His-tagged and purified using HisPur Ni-NTA resin (Thermo Fisher Scientific, 88222). Cell supernatants were diluted with 1/3 volume of wash buffer (20 mM imidazole ...
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bioRxiv - Genetics 2023Quote: ... The Hi-C library is quantified using the Qubit DNA high sensitivity kit (Thermo Scientific, cat. Q32851) on a Qubit 2 fluorometer (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... lysates were incubated for 1hour at 4 °C with His-Pure Cobalt Purification beads (Thermo Fisher Scientific). After incubation ...
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bioRxiv - Bioengineering 2023Quote: ... 6X-His Tag Monoclonal Antibody (HIS.H8) and Goat Anti-Mouse IgGH&L (HRP) were preferred (Thermo Scientific, Cat.#MA1-21315 ...
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bioRxiv - Physiology 2024Quote: ... with Radiant Probe Hi-ROX qPCR Kit (Alkali Scientific) and Taqman Gene Expression Assays (Thermo Fisher Scientific). The expression of target genes was normalized to Gapdh expression.
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bioRxiv - Microbiology 2024Quote: His-tagged recombinant full-length SdeC derivatives were adsorbed to Ni-NTA (Thermo Fisher Scientific, Cat# 88221) resin at 1μg enzyme per 25 μl of packed resin in 1X ART buffer for 1 hr at 4°C with end-over-end rotation ...
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bioRxiv - Microbiology 2024Quote: His-tagged recombinant SdeC and its derivatives were bound to Ni-NTA (Thermo Fisher Scientific, Cat# 88221) resin at 1 μg enzyme per 25μl of packed resin in 1X ART buffer (20mM Tris 10mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... Rubrum CbbM coding region in a His-bdSUMO plasmid acquired from Addgene65 using OneShotTM Top10 cells (Invitrogen). The constructs were expressed in OneShotTMBL21-AITM (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... The Hi-C library is quantified using the Qubit DNA high sensitivity kit (Thermo Scientific, cat. Q32851) on a Qubit 2 fluorometer (Thermo Scientific ...
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bioRxiv - Immunology 2024Quote: ... His-tagged proteins are purified from cell culture supernatants using Ni-NTA agarose beads (Thermo Scientific Pierce) and IgG1 antibodies are purified using protein A agarose beads (Thermo Scientific Pierce) ...
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bioRxiv - Cell Biology 2024Quote: ... PcPotD-cpsfVenus-E284 was synthesized (Integrated DNA Technologies) and cloned into the pBAD/His-B vector (Invitrogen) using Gibson assembly.
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bioRxiv - Molecular Biology 2020Quote: ... at 200 V for 40 min in NuPAGE 2-(N-morpholino)ethanesulfonic acid (MES) running buffer (cat. no. NP0002, Invitrogen), supplemented with NuPAGE antioxidant (cat ...
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bioRxiv - Biochemistry 2021Quote: ... Reaction products were separated by gel electrophoresis on 4-12% Bis-Tris gradient gels using MES SDS running buffer (Invitrogen) and stained with Flamingo fluorescent protein stain (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... The obtained protein samples were separated on 4-12% Bis-TRIS NuPAGE gel in 1 × MES buffer (Thermo Fisher Scientific) and transfer to nitrocellulose membrane before blocking with 5% non-fat milk dissolved in 1 × TRIS-buffered saline (TBS ...
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bioRxiv - Microbiology 2021Quote: Proteins were separated by electrophoresis using a NuPAGE 4-12% Bis-Tris gradient gel run in MES buffer (Life Technologies) and transferred to a Hybond-P PVDF membrane (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were run in triplicate on a 4-12% Bis-Tris gel in 1X MES SDS running buffer for 40 minutes at 200V (all Invitrogen). Three biological replicates were run for each of the four groups ...
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bioRxiv - Molecular Biology 2022Quote: ... Removal of the His6 tag was assessed by running “pre-thrombin” and “post-thrombin” samples on a 4-12% BOLT SDS PAGE gel in MES buffer (Invitrogen); the difference in size between the His6-tagged and untagged version was clearly resolved on these gels ...
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bioRxiv - Immunology 2020Quote: ... mannan-DAP was resuspended at a concentration of 10 mg/1 ml of BupH MES conjugation buffer pH 4.5 (ThermoFisher Scientific) and allowed to solvate for at least 24 hours ...
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bioRxiv - Developmental Biology 2022Quote: ... The 10xUAS:hopx-pA construct was generated using PCR amplified hopx cDNA cloned into a middle entry (ME) vector (pDONR-221) using Gateway technology (ThermoFisher). Subsequent LR reactions were performed combining p5E-10xUAS ...
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bioRxiv - Microbiology 2020Quote: ... Extracted proteins were separated on NuPAGE™ Novex® 4-12% Bis-Tris protein gels in MES buffer (Life Technologies) and transferred onto nitrocellulose membranes using the iBlot® system (Life Technologies) ...
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bioRxiv - Neuroscience 2020Quote: ... the mES cells were dissociated with Trypsin-EDTA and resuspended in EB medium (DMEM/F12: Neurobasal = 1: 1 Medium (Invitrogen), 10% Knockout Serum Replacement (Gibco) ...
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bioRxiv - Molecular Biology 2021Quote: ... with 10% β-mercaptoethanol at 70°C for 10min and separated by gel electrophoresis (NuPAGE 4-12% Bis-Tris gel with 1X MES running buffer, ThermoFisher). Gels were transferred to nitrocellulose membranes (ThermoFisher) ...
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bioRxiv - Pathology 2021Quote: ... 35 mins in MES buffer for aSyn detection) and transferred to nitrocellulose membranes (0.2 μm) via Iblot (7 mins, 20 mV; Fisher Scientific). Initial blots were tested for optimisations of aSyn retention as labelled by MJFR1 either by pre-blocking heat treatment (microwaved ...
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bioRxiv - Immunology 2020Quote: ... they were crushed through a cell strainer and washed before being re-suspended in culture media (RPMI containing 10% FBS, Penicillin/Streptomycin, 0.1% β-ME, 20mM HEPES and L-Glutamine) (media and supplements from ThermoFisher) at the desired density of 107/ml of which 2.5×106 were used for subsequent flow cytometry experiments ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then separated by electrophoresis for a short amount of time (5 min) at 200 V on a NuPAGE 4-12% Bis-Tris gel in 1X MES/SDS (Invitrogen) running buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... the supernatants were processed for loading to Bis-Tris 4-12% gradient Bolt PAGs using Bolt loading dye solution and reducing agents and electrophoresis in MES running buffer (all from Thermofisher) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... After centrifugation (15,000rpm at 4°C for 10 minutes) samples were boiled in Laemmli buffer and loaded into a 10% MES NuPage Bis-Tris gel (Invitrogen) with the Novex™ SharpPre-stained Protein Standard (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: Proteins were separated by electrophoresis using a NuPAGE 4-12% Bis-Tris gradient gel run in MES buffer (Life Technologies) and transferred to a Hybond-P PVDF membrane (GE Healthcare ...
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bioRxiv - Molecular Biology 2022Quote: Proteins were separated by electrophoresis using a NuPAGE 4-12% Bis-Tris gradient gel run in MES buffer (Life Technologies) and transferred to a Hybond-P PVDF membrane (GE Healthcare ...