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Citations for Thermo Fisher :
101 - 150 of 308 citations for rac Clopidogrel MP Endo Derivative 13C d3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: HepG2 (ATCC HB-8065) and derivatives were cultured in low-glucose DMEM (1 g/L, ThermoFisher) with 10% FBS (Axenia BioLogix ...
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bioRxiv - Microbiology 2023Quote: ... and HFF or MRC5 derivative cell lines were cultured in Dulbecco’s modified Eagle medium (DMEM; Invitrogen); ARPE19 retinal epithelial cells were cultured in Roswell Park Memorial Institute 1640 medium (RPMI ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Reagents and solvents for the synthesis of loxapine derivatives were obtained from Acros Organics (Gel, Belgium), Alfa Aesar GmbH & Co KG (Karlsruhe ...
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bioRxiv - Microbiology 2023Quote: ... NIH) and HepG2.C3A and their derivatives were cultured in Dulbeccos’s Modified Eagle Medium (DMEM, Gibco) + GlutaMAX-I supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2024Quote: Plasmids of pET28a-phop or pET28a-phop derivative were first transformed into BL21(DE3) (Invitrogen, Inc.). Then ...
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bioRxiv - Cancer Biology 2024Quote: ... MCF10A and derivative cell lines were maintained in DMEM/F-12 Media (Invitrogen, Cat#10565-018) supplemented with 5% Horse Serum (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... The melting point was calculated by the first derivative using Protein Thermal Shift Software (Applied Biosystems).
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bioRxiv - Physiology 2020Quote: Samples were analyzed for 13C-enrichment using an Elemental Analyser (Flash 2000, Thermo scientific, Milan, Italy) coupled to an Isotopic Ratio Mass Spectrometer (IRMS Delta V Plus with a Conflo IV Interface ...
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bioRxiv - Cell Biology 2022Quote: ... The U-13C Glucose-containing treatment media was made from glucose-free RPMI (Gibco 11879-020) supplemented with 10% heat-inactivated fetal bovine serum (Sigma ...
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bioRxiv - Neuroscience 2024Quote: Endo-MitoEGFP mice received an intravenous injection of the mitochondrial superoxide indicator MitoSOX-Red (33 µM, Life Technologies, Eugene, OR), eyes were collected two hours later ...
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bioRxiv - Neuroscience 2021Quote: ... NSC media was switched to “D3” differentiation media (DMEM/F12 Gibco #11330032; 0.5X B27+ supplement Gibco #17504-044, 1X N2 supplement Gibco #17502-048 ...
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bioRxiv - Neuroscience 2020Quote: ... hCMEC/D3s were washed three times with room temperature (RT) PBS and lysed using Pierce RIPA buffer (Thermo Scientific) containing 1x Halt protease inhibitor cocktail (Thermo Scientific ...
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bioRxiv - Systems Biology 2022Quote: ... at a density of 0.25 x 106 cells/cm2 and cultured for three (d3) or six (d6) days in DMEM/F12 (Gibco) containing 10% FBS and 0.05 mg/ml gentamycin (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... D3 cells were cultured in DMEM/F12 (3:1) medium supplemented with 10% fetal bovine serum (Thermo Fisher Scientific), 8 ng/mL Cholera Toxin (CELL technologies) ...
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bioRxiv - Cell Biology 2024Quote: ... The hcMEC / D3 cells were transfected with HBA1 RNAi plasmid mixed with LipofectamineTM 3000 and P3000TM (Invitrogen / Thermo Fisher) in advance for 24 hours ...
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bioRxiv - Cell Biology 2024Quote: ... The hcMEC / D3 cells were transfected with HBA1 RNAi plasmid mixed with LipofectamineTM 3000 and P3000TM (Invitrogen / Thermo Fisher) in advance for 24 hours ...
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bioRxiv - Cell Biology 2020Quote: ... MCF10A and its derivatives were cultured in DMEM/F12 supplemented with 5% horse serum (GIBCO, #16050-122), 20 ng/ml EGF (Peprotech ...
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bioRxiv - Cancer Biology 2022Quote: H1299 and Mia-PaCa2 cells and their derivatives were cultured in DMEM (Dulbecco’s modified Eagle’s medium, Invitrogen) supplemented with 10% fetal bovine serum (Hyclone) ...
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bioRxiv - Microbiology 2024Quote: HEK293T cells (ATCC, CRL-1586) and their derivatives were maintained in Dulbecco’s modified eagle medium (DMEM; Gibco) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2023Quote: ... The dyes used for labelling were NHS ester derivatives: Alexa Fluor 405 Carboxylic Acid Succinimidyl Ester (Invitrogen), Cy3 mono-Reactive Dye Pack (GE HealthCare) ...
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bioRxiv - Molecular Biology 2023Quote: Diluted SCFA derivatives (10 µL) were injected into an RSLC UltiMate 3000® system (Thermo Fisher Scientific) coupled with a QTRAP 5500® mass spectrometer (AB Sciex ...
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bioRxiv - Genetics 2024Quote: ... female origin) and derivatives were maintained at 37°C and 5% CO2 in DMEM/F-12 (Gibco), 10% fetal bovine serum (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: His-tagged recombinant full-length SdeC derivatives were adsorbed to Ni-NTA (Thermo Fisher Scientific, Cat# 88221) resin at 1μg enzyme per 25 μl of packed resin in 1X ART buffer for 1 hr at 4°C with end-over-end rotation ...
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bioRxiv - Microbiology 2024Quote: His-tagged recombinant SdeC and its derivatives were bound to Ni-NTA (Thermo Fisher Scientific, Cat# 88221) resin at 1 μg enzyme per 25μl of packed resin in 1X ART buffer (20mM Tris 10mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... equal amounts of protein were submitted to Endo H treatment or mock-treated followed by SDS-PAGE analysis and transferred into a PVDF transfer membrane (ThermoFisher Scientific) as previously described (Duart et al. ...
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bioRxiv - Cell Biology 2022Quote: ... Both the LentiCRISPRv2-sgRNA plasmid and the ss donor oligonucleotides were transfected into D3 mouse ESC line using Lipofectamine 3000 (Invitrogen). Transfected cells were selected with puromycin (1.5 μg/ml ...
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bioRxiv - Biochemistry 2021Quote: ... Both the LentiCRISPRv2-sgRNA plasmid and the ss donor oligonucleotides were transfected into D3 mouse ES cell line using lipofectamine 3000 (Invitrogen). Transfected cells were selected with puromycin (1.5 μg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... n=15 full larvae) or extracted from hCMEC/D3 cells seeded in 12 well/plates using a standard TRIzol (Invitrogen) method ...
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bioRxiv - Physiology 2024Quote: Cells from the human brain microvascular EC line hCMEC/D3 [37] were seeded on collagen I-coated 35 mm tissue culture dishes (Nunc) and grown in EGM2-MV (Lonza).
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bioRxiv - Cell Biology 2020Quote: ... MCF-10A cells (ATCC, Manassas, VA, USA) and derivatives were cultured in DMEM/F12 (Gibco, Waltham, MA, USA) supplemented with 5% horse serum (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... The thiamine and TPP derivatives were measured using a Dionex UltiMate 3000 fluorescence detector (Thermo Fisher Scientific, USA) with 375 nm excitation and 450 nm emission ...
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bioRxiv - Cell Biology 2022Quote: ... MC3T3 and derivatives were maintained in Minimum Essential Medium α (MEM-α) without ascorbic acid (Thermo Fisher, A1049001) with 10% FBS ...
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bioRxiv - Cell Biology 2021Quote: ... MDA-MB-231 cells (ATCC) and derivatives were cultured in Dulbecco’s Modified Eagle’s Medium with 10% fetal bovine serum (Invitrogen) and 1% penicillin/streptomycin ...
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bioRxiv - Cell Biology 2021Quote: ... gondii tachyzoites parental and derivative strains were grown in confluent human foreskin fibroblasts (HFFs) maintained in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) supplemented with 5% fetal calf serum (FCS) ...
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bioRxiv - Microbiology 2022Quote: Human A549 cells (ATCC CCL-185) and its derivatives were cultured in RPMI 1640 (Gibco catalog no. 11875) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2020Quote: The murine PUER cell line and its derivatives were maintained in phenol red-free Iscove’s Modified Dulbecco’s Medium (Gibco), supplemented with 10% HI-FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... and derivatives thereof were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12; Gibco, #11320-033 or Corning Cellgro ...
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bioRxiv - Cell Biology 2020Quote: 4T07 cells (ATCC) and derivatives were cultured in Dulbecco’s Modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (Invitrogen), 1% non-essential amino acids (Thermo ...
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bioRxiv - Immunology 2020Quote: ... and their knock-out derivative clones were maintained in a 50:50 mixture of Dulbecco’s modified Eagle’s medium (DMEM) and F12 (GibCo) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Biochemistry 2023Quote: ... alanine derivatives were used in a concentration of 10 mM and RNaseOUT recombinant ribonuclease inhibitor (1 µl) (Invitrogen) was added ...
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bioRxiv - Biochemistry 2023Quote: Human HAP1 derivative reporter cell line (HAP1-AAVS-EGFP, Tan et al., 2022) were cultured in IMDM (Gibco) supplemented with 10% FBS (Corning ...
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bioRxiv - Microbiology 2024Quote: ... These cells and their derivatives were maintained in complete Dulbecco’s modified Eagle’s medium (DMEM) (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum ...
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bioRxiv - Microbiology 2024Quote: Human A549 cells (ATCC CCL-185) and its derivatives were cultured in RPMI 1640 (Gibco catalog no. 11875) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Physiology 2023Quote: 13C glucose or 12C glucose of the same molar concentration was added to Glucose-free DMEM (Gibco A1443001) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2024Quote: ... and their isotopic 13C/12C ratios were measured on a gas chromatograph (Trace GC-Ultra, Thermo Fisher Scientific) coupled to an isotope ratio mass spectrometer (Finnigan Delta-V Advantage ...
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bioRxiv - Cell Biology 2024Quote: ... colonies containing the correct construct were identified by sequencing the purified plasmid (GeneJET Endo-free Plasmid Maxiprep Kit, Thermo Fisher cat. #K0861), which was later employed for the transfection of hippocampal neurons ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Both of the in vitro systems depend on the application of three-dimensional embryoid body cultures derived from either mouse embryonic stem cells (mESC, ES-D3, ATCC, CRL-1934) or human episomal induced pluripotent stem cells (hiPSC, Gibco, A18945). The teratogenicity determination of the mEST is based on the half maximal inhibition of differentiation into beating cardiomyocytes (ID50 ...
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bioRxiv - Neuroscience 2021Quote: ... or with a pool of two different USP30 siRNAs (D1: 5’-CAAAUUACCTGCCGCACAA-3’; D3, 5’-ACAGGAUGCUCACGAAUUA-3’, Dharmacon; siUSP30) by using Lipofectamine RNAiMAX (Thermo Scientific) following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: Clathrin and caveolin-1 knockdown in polarized hCMEC/D3 cell monolayers was performed using siRNA kit containing RNAi mix (Invitrogen, CA) and reduced serum medium ...
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bioRxiv - Neuroscience 2022Quote: ... GFP and non-GFP K44-negative-dominant dynamin transfections of hCMEC/D3 cell monolayers were performed using Lipofectamine reagent (Invitrogen, CA) as per manufacturer’s protocol ...