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Citations for Thermo Fisher :
101 - 150 of 283 citations for pcDNA5 FRT since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... which was generated by inserting EGFP into the KpnI and HindIII restriction sites of pcDNA5/FRT/TO (Invitrogen). For baculoviral expression of His10-USP36-TEV-St (TEV ...
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bioRxiv - Biochemistry 2021Quote: Flag-tagged UCH37 (WT, C88A, ΔCTD, ΔCTD/C88A, EWI, EWI/C88A) were cloned into pcDNA5/FRT/TO (Invitrogen). Stable cell lines that inducibly express these proteins were constructed from Flp-InTM T-RExTM 293 Cells following manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Human cMyc-mGlu1 cDNA sequence was subsequently transferred from pcDNA3.1(+) into the destination vector pcDNA5/FRT/TO (Invitrogen) using NEBuilder HiFi assembly (New England Biolabs ...
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bioRxiv - Biophysics 2022Quote: ... by PCR and inserted into the BamHI and XhoI restriction sites of the pcDNA5-FRT/TO plasmid (Invitrogen).
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bioRxiv - Bioengineering 2023Quote: ... The insert was then ligated with the pcDNA5/FRT vector using T4 DNA ligase (Thermo Fisher Scientific, USA) following standard sticky end ligation protocol ...
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bioRxiv - Neuroscience 2024Quote: HA tagged human Q331K mutant TDP-43 was cloned in the pcDNA5/FRT/TO plasmid (Invitrogen™ V652020) and sequence verified ...
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bioRxiv - Biophysics 2024Quote: ... the fusion construct was subcloned into a pcDNA5/FRT/TO vector (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA). All plasmid sequences were verified by Sanger sequencing (Microsynth AG ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with the relevant pcDNA5/FRT/TO directSLiMs plasmid and the Flp recombinase pOG44 (Thermo Fisher) using Fugene HD (Promega ...
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bioRxiv - Immunology 2021Quote: ... The vector used was a variant of the pCDNA5/FRT mammalian expression vector (Thermo Fisher Scientific, Waltham, MA, USA). The vector has an elongation factor 1 alpha (EF1α ...
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bioRxiv - Cell Biology 2021Quote: ... were generated by Gateway Cloning into pcDNA5 FRT-TO (3X FLAG N-terminus or EGFP N-terminus) vectors (Invitrogen). Point mutations and truncations were generated by site-directed mutagenesis using the QuikChange II Site-Directed mutagenesis kit (Agilent #200524 ...
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bioRxiv - Microbiology 2020Quote: ... gene id: 1502173) was cloned from the plasmid pACNR-FLYF17D-Da plasmid into pcDNA5/FRT/TO (ThermoFisher Scientific, #V652020) and pET303/CT-His vector (ThermoFisherScientific ...
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bioRxiv - Molecular Biology 2021Quote: ... The plasmid contains the CFTR gene coding sequence in the pCDNA5 FRT/TO plasmid backbone (Life Technologies, Carlsbad, CA). The CFTR coding sequence with a C-terminal T7 tag from pcDNA5.FRT-wtCFTR was cloned into the multiple cloning site of the pCW57-GFP-2A-MCS plasmid ...
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bioRxiv - Molecular Biology 2023Quote: ... The cloned sequence was verified by sequencing and it was transferred to the pcDNA5-FRT-TO-N-GFP Gateway destination vector by LR recombination according to the manufacturer’s protocol (Invitrogen). HeLa Flp-In/T-REx cells ...
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bioRxiv - Molecular Biology 2022Quote: ... These sequences were PCR-amplified and introduced between HindIII and NotI sites in the pcDNA5/FRT/TO plasmid (Thermofisher). For subsequent mutagenesis or modifications ...
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bioRxiv - Cell Biology 2023Quote: ... pcDNA5/FRT/TO N-YFP vector was double digested by restrictive enzymes of KpnI and NotI (Thermo Fisher Scientific). Wild type Cdc20 was amplified by PCR and inserted into the expression vector according to standard procedures ...
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bioRxiv - Neuroscience 2023Quote: ... These sites were then utilized to clone the reporter construct into the pCDNA5 FRT multi-cloning site (ThermoFisher, V601020).
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bioRxiv - Biochemistry 2022Quote: ... HEK293 cell lines were transfected with pOG44 and pcDNA5/FRT/TO plasmids harboring respective FLAG constructs using Lipofectamine3000 (Invitrogen) as transfection reagent according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... University of Birmingham) cells transfected with pcDNA5/FRT/TO based vectors (Supplementary Table 2) and the recombinase pOG44 (Invitrogen) using FuGene6 (Promega ...
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bioRxiv - Molecular Biology 2022Quote: ... University of Birmingham) cells transfected with pcDNA5/FRT/TO based vectors (Supplemental table 2) and the recombinase pOG44 (Invitrogen) using FuGene6 (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... CENP-E constructs were cloned into a pCDNA5/FRT/TO-EGFP-IRES plasmid and co-transfected with pOG44 (Invitrogen), encoding the Flp recombinase ...
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bioRxiv - Cell Biology 2024Quote: The coding region of full-length LAP2β was amplified from pEGFP-N3-LAP2β105 and cloned into KpnI and NotI sites of pCDNA5/FRT/TO (Invitrogen), which had been modified by inserting the EGFP coding sequence into the XhoI and ApaI restriction sites ...
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bioRxiv - Cell Biology 2020Quote: ... The Flp-In T-Rex HEK293 cells were transfected with the N-terminal GFP-tagged FAM83F packaged in a pcDNA5-FRT/TO vector along with Flp recombinase pOG44 (Invitrogen) in a ratio of 1 μg ...
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bioRxiv - Cell Biology 2020Quote: ... were PCR amplified with BamHI and NotI sites in the forward and reverse primers and the products cloned into pcDNA5/FRT/TO (Invitrogen) containing GFP or GFP-H2B ...
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bioRxiv - Immunology 2021Quote: GIGA-564 was expressed from a variant of the pCDNA5/FRT mammalian expression vector (Thermo Fisher Scientific; Waltham, MA, USA). The vector has a promoter for glutamine synthetase selection and uses an EF1α promoter to drive light chain expression ...
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bioRxiv - Molecular Biology 2020Quote: ... Wild type and catalytic mutant H672N RNase L plasmids cDNAs (the backbone is pcDNA5-FRT-TO with CMV promoter, Invitrogen) used in this study were a generous gift from Dr ...
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bioRxiv - Molecular Biology 2020Quote: ... The wild-type complemented HEK293 RAD51 S14A mutant was generated by co-transfecting pcDNA5/FRT encoding wild-type RAD51 and pcDNA-DEST26 (Invitrogen), followed by single cell sorting (FACS ...
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bioRxiv - Immunology 2021Quote: ... each mouse Siglec gene containing 2-3 domains with pairs of forward and reverse primers (Table S5) were cloned into pcDNA5/FRT/V5-His-TOPO vector (Invitrogen), which contained a C-terminal hIgG1-Fc ...
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bioRxiv - Biochemistry 2021Quote: ... were fused to the C terminus of YFP by cloning the DNA encoding this into the pcDNA5/FRT/TO vector (Invitrogen). DNA encoding the G3BPi sequences was purchased from GeneArt ...
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bioRxiv - Biochemistry 2021Quote: ... M-OPG2 and S-OPG2 were generated by inserting the respective cDNAs in frame between NheI and AflII sites of a pcDNA5/FRT/V5-His vector (Invitrogen) containing a C-terminal OPG2 tag (MNGTEGPNFYVPFSNKTG) ...
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bioRxiv - Neuroscience 2020Quote: ... an enhanced GFP and human WT 0N4R tau sequences were inserted between the BamHI and XhoI restriction sites on the pcDNA5/FRT/TO plasmid (Invitrogen). A short linker sequence (ATCGATGCA ...
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bioRxiv - Cell Biology 2021Quote: ... were inserted without their respective stop codons between the BamHI and the XhoI restriction sites of the pcDNA5/FRT/TO vector (Invitrogen) using standard cloning techniques ...
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bioRxiv - Immunology 2021Quote: ... with a T4 trimerization domain and a 6xHis tag at the C-terminus were codon-optimized and were subcloned into pcDNA5/FRT mammalian expression vector (ThermoFisher). Expi293 Expression system (ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293T cells stably expressing FLAG/HA-tagged human PRPF4 was generated using the pcDNA5/FRT-PRPF4 plasmid and Flp-In-293 cells according to the manufacturer’s protocol (Thermo Fisher). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... Flp-In-293 cells were transfected with a 9:1 ratio of pOG44:pcDNA5/FRT-PRPF4 plasmids using Lipofectamine 2000 (Invitrogen) and 48 h post-transfection ...
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bioRxiv - Biochemistry 2022Quote: ... The amplified cDNA fragment and a DNA fragment coding V5SBP were cloned into pcDNA5/FRT/TO vector (Thermo Fisher Scientific) by In-fusion cloning (Takara) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Stable Flp-In-CHO cell lines were generated through co-transfection of the pcDNA5/FRT expression vector (Thermo Fisher Scientific) containing the gene of interest and the Flp recombinase expressing plasmid ...
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bioRxiv - Cell Biology 2020Quote: ... For stable expression of GFP-tagged mouse RHBDL4-SA and RHBDL4-SAUIM, the open reading described previously (Fleig et al., 2012) were sub-cloned into pcDNA5/FRT/TO (Invitrogen). For transient knockdown the small hairpin (shRNA)-expressing vector pSUPER.neo targeting OST4 (targeting sequence ...
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bioRxiv - Cell Biology 2020Quote: ... eGFP-APC2 and APC3-eGFP into the genome the corresponding genes were cloned into the pcDNA5-FRT-TO vector (Invitrogen) with an N-terminal or C-terminal (only in the case of APC3 ...
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bioRxiv - Cell Biology 2021Quote: ... Tetracycline-inducible cell lines were generated by stable integration of pcDNA5-based constructs into the FRT site upon co-transfection with pOG44 plasmid (Invitrogen). HeLa FRT cells (Hafner et al. ...
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bioRxiv - Biochemistry 2021Quote: DNA sequences encoding N-terminally GFP-fused and truncated FAM98B constructs were cloned into the pcDNA5/FRT/TO/nHASt vector (Invitrogen). The resulting FAM98B truncations contained an N-terminal HA-(StrepII)2-GFP tag ...
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bioRxiv - Cell Biology 2020Quote: ... For site-specific integration of tetracycline-inducible pCDNA5 FRT/TO constructs the pOG44 plasmid encoding Flp-recombinase (Thermo Fisher Scientific) was co-electroporated according to the manufactures instructions using a Neon transfection system (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2021Quote: ... norvegicus with a HA-tag added to the C-terminal end representing the N-terminal domain (Met 1 to Val 469) of sAC (57) was cloned into pcDNA5/FRT (Invitrogen).
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bioRxiv - Cell Biology 2023Quote: ... HeLa cell lines with single integrated copies of the desired NDC80-GFP transgene in pcDNA5/FRT/TO were created using the doxycycline-inducible T-REx Flp-In system (Invitrogen). Transgene expression was induced by addition of 1µg/ml doxycycline (Merck ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequences encoding KNL12026-2342 and ZWINT wild-type and mutants were cloned into a pCDNA5/FRT/TO-EGFP-IRES plasmid and co-transfected with pOG44 (Invitrogen), encoding the Flp recombinase ...
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bioRxiv - Molecular Biology 2022Quote: pcDNA5/FRT/TO-FLAG-Angel1 WT was used to generate stable HEK FLp-In T-REx-293 cell lines (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... pcDNA5/FRT/TO/GFP/TOPORS plasmids were co-transfected with pOG44 into U2OS Flp-In T-Rex cells (ThermoFisher Scientific) using Lipofectamine3000 (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... APC8-mScarlet+/+ or RPE-1 FRT/TO APC8-mScarlet+/+ cell lines were cotransfected with the relevant gene CDS cloned into pcDNA5-FRT/TO (p1795) and pOG44 (Invitrogen) using a 1:5 ratio (using 1 µg total DNA per million cells) ...
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bioRxiv - Cell Biology 2022Quote: ... CMV-mPiezo1 was previously generated in-house by subcloning mouse Piezo1 into the pcDNA5/FRT expression vector (Fisher Scientific, V601020) – this plasmid was used for all calcium imaging of Piezo1 activity ...
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bioRxiv - Molecular Biology 2023Quote: ... a synthetic DNA fragment of EGFP-L10a was cloned into a expression vector pcDNA5/FRT/TO (V652020, Thermo Fisher Scientific). Although the human ribosomal protein L10a is referred to as uL1 in standard nomenclature (36) ...
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bioRxiv - Molecular Biology 2024Quote: ... using a pDSG-IBA-Twin-Strep-Tag vector as template (IBA-lifesciences) into the HindIII-ApaI site of pcDNA5-FRT-TO (Invitrogen).