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Citations for Thermo Fisher :
101 - 150 of 10000+ citations for SARS CoV 2 Nucleoprotein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The pBAC-SARS-CoV-2-mCherry-2A was digested with BstBI (FastDigest, Thermo Fisher) and BamHI (FastDigest ...
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bioRxiv - Microbiology 2023Quote: SARS-CoV-2 S2 variants were transiently transfected in ExpiCHO-S cells (Thermo Fisher). CHO cells were maintained and transfected according to the manufacturer’s protocols ...
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bioRxiv - Bioengineering 2022Quote: SARS-CoV-1 (Genbank AAP13567) or SARS-CoV-2 (Genbank MN908947) S proteins were transiently expressed in Freestyle 293F system (ThermoFisher) whereas RBD proteins were expressed in the Expi293 system (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: Total RNA was extracted from the SARS-CoV-2 infected Caco-2 cells using Trizol (Thermo Fisher). The extracted RNA was then reverse transcribed with the reverse primers specific to Nsp12 ...
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bioRxiv - Microbiology 2022Quote: ... pylori NAP proteins (SARS-CoV-2S6p3 and SARS-CoV-2S6p312, respectively) were produced upon transient expression in Expi293F cells (ThermoFisher). Clarified supernatants were purified by affinity chromatography using an anti-HA affinity matrix (Cat# 11 815 016 001 ...
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bioRxiv - Biophysics 2022Quote: ... Escherichia coli (E. coli) BL21-AI cells (Invitrogen) were transformed with a modified pET vector encoding N-terminal 10X His tagged VcINDY ...
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bioRxiv - Immunology 2021Quote: ... with SARS-Cov-2 spike plasmid or a control plasmid using Lipofectamine 2000 (Life technologies) are kind gifts from Olivier Schwartz ...
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bioRxiv - Biochemistry 2020Quote: ... and SARS-CoV-2 [3]) was performed with Proteome Discoverer version 2.4 (Thermo Fisher Scientific). The search was restricted to human and SARS-COV-2 proteins ...
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bioRxiv - Microbiology 2022Quote: ... human Anti-SARS COV-2 Nucleocapsid monoclonal (E16C) (Thermo Fisher, MA1-7403, 0.1 mg/mL), was incubated as a 1:100 dilution ...
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bioRxiv - Immunology 2020Quote: ... Recombinant SARS-CoV-2 RBD protein was coupled to fluorescent beads (Thermo Scientific, Cat # F8819) by carbodiimide coupling ...
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bioRxiv - Microbiology 2020Quote: ... the purified SARS-CoV-2 RBD protein was coated on Nunc MaxiSorp immuno tubes (ThermoFisher) at around 5μg/mL in PBS overnight ...
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bioRxiv - Microbiology 2020Quote: ... codon-optimized (for human cells) SARS-CoV-2 DNA (GeneArt Gene Synthesis, Thermo Fisher Scientific) based on the publicly available protein sequence in the National Center for Biotechnology Information database (NCBI Reference Sequence ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stained with mouse anti-SARS-CoV-2 N (#MA5-29981; Thermo Fisher Scientific) and anti-mouse IgG Alexa488 (#A-11029 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Validation and SARS-CoV-2 genome detection was performed with TaqMan detection systems (Applied Biosystems) based qPCR using SARS-CoV-2 specific primers and probes with the following sequences ...
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bioRxiv - Biochemistry 2021Quote: ... The SARS-CoV-2 spike glycoprotein RBD was expressed in Freestyle 293 cells (ThermoFisher, MA) grown in Freestyle 293 media at 37 °C with 8% CO2 and shaking at 135 rpm in a humidified incubator ...
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bioRxiv - Microbiology 2021Quote: ... the purified SARS-CoV-2 RBD proteins were coated on Nunc MaxiSorp immuno tubes (ThermoFisher) at 5μg/mL in PBS overnight ...
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bioRxiv - Immunology 2021Quote: ... RBD binding inhibition was measured using a SARS-CoV-2 Neutralizing Ab ELISA Kit (Invitrogen). For anti-Spike IgG experiments ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 RBD 6xHisTag constructs were expressed by transient transfection of Expi293F cells (Gibco) and purified using HisTrap FF and HisTrap HP columns (GE Healthcare Life Sciences) ...
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bioRxiv - Immunology 2020Quote: ... plasmid DNA (pVAX1 or pVAX1-SARS-CoV-2 Spike) was transfected with Lipofectamine 2000 (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 pseudoparticles were produced by co-transfecting HEK293T cells using Lipofectamine 2000 (Invitrogen) with plasmids encoding HIV-1 gag/pol ...
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bioRxiv - Pathology 2022Quote: ... and either pMD2.G or pTwist-EF1alpha-SARS-CoV-2-S-2xStrep using LTX (Invitrogen) transfection reagents according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 infected cells were washed with PBS and lysed with TRIzol reagent (Invitrogen). Total RNA was extracted using RNeasy mini kit (QIAGEN ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 spike variant proteins in PBS pH7.4 were immobilised on Maxisorb ELISA (Nunc) plates at a concentration of 2 μg/mL overnight ...
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bioRxiv - Biochemistry 2023Quote: ... SARS-CoV-2 spike variant proteins in PBS pH7.4 were immobilised on Maxisorb ELISA (Nunc) plates at a concentration of 2 μg/mL overnight ...
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bioRxiv - Immunology 2024Quote: ... indicated SARS-CoV-2 Z-RNAs) was incubated with Lipofectamine 2000 transfection reagent (Invitrogen, 11668019), for 15 mins at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... 2 μg of N501Y-SARS-CoV-2 BAC were transfected into Vero E6 (ATCC) with Lipofectamine 3000 (Invitrogen) in a 6-well plate according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: Dynabeads His-Tag Isolation & Pulldown (Invitrogen) were washed in PBS-TH (PBS ...
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bioRxiv - Immunology 2021Quote: ... magnetic beads (Dynabeads His-Tag, Invitrogen) were coated with histidine tagged S protein according to the manufacturers’ instructions ...
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bioRxiv - Immunology 2021Quote: 6x His tag monoclonal antibody (Invitrogen) was coated at 2 μg/mL in PBS onto half-area 96-well high binding plates (Corning ...
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bioRxiv - Immunology 2021Quote: 6x His tag monoclonal antibody (Invitrogen) or streptavidin (Jackson ImmunoResearch ...
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bioRxiv - Biochemistry 2020Quote: ... Anti‐His tag monoclonal antibody (Invitrogen) was used in western blot analysis.
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bioRxiv - Synthetic Biology 2022Quote: ... 2 μl of Dynabeads™ His-Tag Isolation and Pulldown beads (ThermoFisher 10104D) for each CFPS reaction were equilibrated twice in equal volumes of wash buffer before addition to the purified reactions to remove the Ulp-protease and incubated with spinning at 4 °C for at least 30 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... the membrane was incubated overnight with SARS/SARS-CoV-2 Coronavirus Spike Protein (subunit 1) rabbit polyclonal antibody (Thermo Fisher scientific, France) at a dilution of 1:1000 in the same buffer as for saturation ...
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bioRxiv - Immunology 2021Quote: ... residues 306–577 of MERS-CoV S with a C-terminal monomeric human IgG Fc-tag and an 8x HisTag (SARS-CoV RBD-SD1) were transiently transfected into FreeStyle293F cells (Thermo Fisher) using polyethylenimine ...
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bioRxiv - Bioengineering 2022Quote: ... or SARS-CoV-1-d28 (Genbank AAP13567) or SARS-CoV-2 VOC spike plasmid were incubated with transfection reagent Lipofectamine 2000 (Thermo Fisher, 11668027) following manufacturer’s instructions for 20 min at RT ...
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bioRxiv - Bioengineering 2019Quote: ... fluorescent Escherichia coli (E. coli) BioParticles (ThermoFisher, cat. #E2863), or fluorescent carboxylate-modified 1 µm polystyrene beads (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: Viral RNA from SARS-CoV-2 infected HBEC cell lysates was extracted using TRIzol (Life Technologies) and purified using Direct-zol RNA MiniPrep Plus according to manufacturer’s instructions (Zymo Research ...
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bioRxiv - Molecular Biology 2020Quote: ... complementary cDNA of SARS-CoV-2 clinical samples was generated using SuperScript II Reverse Transcriptase (Invitrogen), random primers and 10μL of RNA ...
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bioRxiv - Bioengineering 2021Quote: Both Nefmut/SARS-CoV-2-based fusion proteins were cloned into the pVAX1 plasmid (Thermo Fisher) as already described (22) ...
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bioRxiv - Microbiology 2021Quote: ... The purified bacmid of SARS-CoV-2 was used for transfection using cellfectin (Thermo Fisher, SG). The viral stocks obtained from transfection were amplified and expressed for protein production ...
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bioRxiv - Neuroscience 2020Quote: ... Bound SARS-CoV-2 Spike was detected by antiLJHis probe HRP (Cat#15165; Thermo Fisher Scientific). Some wells received no antibody (no antibody control) ...
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bioRxiv - Cell Biology 2021Quote: ... employing the Ion AmpliSeq SARS-CoV-2 RNA custom primers panel (ID: 05280253, Thermo Fisher Scientific). Briefly ...
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bioRxiv - Microbiology 2022Quote: All SARS-CoV-2 Spike constructs for pseudotype generation were expressed from plasmid pCDNA3.1 (ThermoFisher #V79020). Codon optimized SARS-CoV-2 Wuhan Spike carrying the D614G amino acid change (Sino Biological #VG40589-UT(D614G) ...
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bioRxiv - Microbiology 2020Quote: ... anti-NeuN and anti-SARS-CoV-2 spike protein antibodies (Thermo Fisher Scientific, Norcross, GA, USA) overnight at 4°C followed by incubation with Alexa Fluor 546- or Alexa Fluor 488-conjugated secondary antibody for 1 hr at room temperature [31,34] ...
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bioRxiv - Molecular Biology 2020Quote: Artificial codon optimized genes encoding SARS-CoV-2 nsp10 and nsp16 proteins were commercially synthesized (Invitrogen) and cloned in a pSUMO vector that encodes an N-terminal His8x-SUMO tag ...
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bioRxiv - Immunology 2020Quote: ... We synthesized oligonucleotide fragments encoding the SARS-CoV-2 gene using GeneArt strings (Thermo Fisher Scientific) and assembled into the plasmid vector with NEB HiFi assembly (New England BioLabs) ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR detection of SARS-CoV-2 was performed on a QuantStudio 5 instrument (Applied Biosystems) using a TaqPath 1-step RT-qPCR Master Mix (Applied Biosystems ...
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bioRxiv - Immunology 2021Quote: ... An anti-S2 SARS-CoV-2 S polyclonal primary antibody (1:1,500 dilution,Invitrogen PA5-114534) and an Alexa Fluor 680-conjugated goat anti-rabbit secondary antibody (1:20,000 dilution ...
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bioRxiv - Immunology 2020Quote: ... and a TwinStrepTag (SARS-CoV-2 HexaPro S) were transiently transfected into FreeStyle293F cells (Thermo Fisher) using polyethylenimine ...
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bioRxiv - Microbiology 2021Quote: A commercially available SARS-CoV-2 Inhibitor Screening Kit (Cat#: 16605302, Fisher Scientific GmbH, Schwerte, Germany) was used for cell free detection of SARS-CoV-2 Spike – ACE2 interaction inhibition ...