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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for SARS CoV 2 Nucleoprotein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 5x-His mouse anti-tag was used at 1:500 (Invitrogen). Anti-mouse HRP secondary antibody was used at 1:10,000 (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... the following antibodies were used: 6x-His Tag monoclonal antibody (ThermoFisher, cat ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µL of His-Tag magnetic beads (Dynabeads™ Invitrogen #10103D) was added to each tube ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were taken for immunoblotting using anti His-tag antibody (ThermoFisher). Crosslinking and control reactions intended for scramblase activity assays were stopped with 1 M Tris pH 7 to a final concentration of 10 mM ...
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bioRxiv - Biophysics 2023Quote: ... To cut the His-tag HRV 3C protease (Thermo-Fisher Scientific) was added to a molar ratio of 1:800 (enzyme ...
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bioRxiv - Developmental Biology 2019Quote: ... 2% (E-Gel, GIBCO). DNA fragments from the complete and size-selected library were detected and confirmed ...
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bioRxiv - Neuroscience 2020Quote: ... coli expression vector pDEST15 with GST tag Gateway Vector (Invitrogen). MYC-LRRK2-WT was a gift from Dr ...
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bioRxiv - Cancer Biology 2020Quote: ... His-tagged GOT2 protein was immunoprecipitated using the His-tag isolation and pull-down Dynabeads system (Thermo Fisher) using the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... whereas His-tagged EF-P was detected with primary monoclonal antibodies against the His-Tag (1:10,000; AB_2536841, Invitrogen). Membranes were incubated with the secondary antibodies conjugated with a fluorophore (1:20,000 in TBS ...
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bioRxiv - Neuroscience 2020Quote: ... and 0.09 μL Ambion RNase H (E. coli) (Invitrogen, AM2293). Second strand synthesis was performed by incubating samples at 16°C for 150 min ...
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bioRxiv - Microbiology 2022Quote: ... Expression of E was detected using an HA-tag antibody (Invitrogen). β-actin was used as a loading control.
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bioRxiv - Microbiology 2022Quote: ... Membranes were next treated with 1: 1000 dilution of primary antibody (6-His tag mouse anti-tag, Invitrogen) for 1 hr at RT ...
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bioRxiv - Developmental Biology 2021Quote: Escherichia coli (E. coli) strain DH5α (Toyobo, Osaka, Japan) and BL21(de3) pLysS (C606003, ThermoFisher Scientific) were used for DNA cloning and protein expression ...
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bioRxiv - Genomics 2022Quote: ... vector and amplified in DH5α strain of Escherichia coli (E coli) (Life Technologies, Grand Island NY). After amplification ...
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bioRxiv - Developmental Biology 2021Quote: Escherichia coli (E. coli) strain DH5α (Toyobo, Osaka, Japan) and BL21(de3) pLysS (C606003, ThermoFisher Scientific) were used for DNA cloning and protein expression ...
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bioRxiv - Microbiology 2024Quote: Glycerol stock of Escherichia coli (E. coli) (TOP10 strain, Invitrogen, Thermo Fisher Science, Waltham, MA, USA) transformed with plasmid pCR2.1 (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... A total of 2 mg of lysate per condition together with 25 μL of DynabeadsTM for His-Tag isolation (Invitrogen) were incubated with His6-AtLEGβ or the His6-proAtLEGβ-C211A dead mutant or lysate alone for an hour at room temperature with rotation ...
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bioRxiv - Microbiology 2022Quote: ... effector cells were stained with rabbit anti-SARS-CoV-2 S S1/S2 polyclonal antibody (Thermo Fisher Scientific, Cat# PA5-112048, 1:100). Normal rabbit IgG (SouthernBiotech ...
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bioRxiv - Biophysics 2021Quote: ... were coated either with 200 ng of SARS-CoV-2 SΔTM proteins or bovine serum albumin (BSA, Thermo Scientific™, Waltham, MA, USA) through incubation overnight at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... 9 x 105 cells of each indicated SARS-CoV-2-non-infectable cell type were stained with CellTrace™ Violet (Thermo Fisher # C34557), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Sequence corresponding to the 5’-300 bases of SARS-CoV-2 was PCR amplified from pUC57 vector and cloned into the pJet1.2 vector (Thermo Fisher Scientific, Massachusetts, USA) under the control of T7 promoter ...
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bioRxiv - Microbiology 2020Quote: ... total cellular RNA containing SARS-CoV-2 RNA was extracted from the Vero E6 cells used for viral stock production using TRIzol™ Reagent (Invitrogen™, ThemoFisher) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Cells were infected in a BSL-3 lab with the UF-1 strain of SARS-CoV-2 at MOI of 4 in media containing 3% low IgG FBS (Fisher Scientific, Cat. SH30070.03).
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bioRxiv - Immunology 2020Quote: The plasmid expressing the soluble pre-fusion version of SARS-CoV-2 S was used to produce the recombinant protein using the FreeStyle™ 293 Expression System (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Zoology 2020Quote: Libraries for whole genome sequencing were generated using the Ion AmpliSeq Kit for Chef DL8 and Ion AmpliSeq SARS-CoV-2 Research Panel (Thermo Scientific, Waltham, MA). Libraries were sequenced using an Ion 520 chip on the Ion S5 system using the Ion 510™ & Ion 520™ & Ion 530™ Kit ...
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bioRxiv - Microbiology 2021Quote: Libraries for the whole genome sequencing were generated using the Ion AmpliSeq Kit for Chef DL8 and Ion AmpliSeq SARS-CoV-2 Research Panel (Thermo Scientific, Waltham, MA). Libraries were sequenced using an Ion 520 chip on the Ion S5 system using the Ion 510™ & Ion 520™ & Ion 530™ Kit ...
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bioRxiv - Microbiology 2021Quote: ... and assembled into full length SARS-CoV-2 cDNA clones in the YAC vector pYESL1 using a GeneArt™ High-Order Genetic Assembly System (A13285, Invitrogen™, ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Four microliters RNA was used for real-time RT-qPCR to detect and quantify N gene of SARS-CoV-2 using TaqMan™ RNA-to-CT 1-Step Kit (Thermo Fisher Scientific) as described [41] using the following primers and probes ...
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bioRxiv - Immunology 2021Quote: ... rVSV-ΔG/SARS-2-S*-luciferase pseudovirus was generated by transfecting BHK21/WI-2 cells with pcDNA expressing codon-optimized full-length SARS-CoV-2 S protein (GenBank accession number: MN908947) using Lipofectamine™ 2000 transfection reagent (Invitrogen, Carlsbad, CA). Transfected cells were then infected with rVSV-ΔG/G*-luciferase 24 h later ...
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bioRxiv - Immunology 2021Quote: ... 100 μL of test peptide (at a final concentration of 1 μg/ml) PepMixTM SARS-CoV-2 S (JPT, PM-WCPV-S-1) or murine anti-CD3 antibody (positive control) (Invitrogen, 16-0031-82) or CTL-TestTM medium (negative control ...
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bioRxiv - Immunology 2020Quote: Seventy-six consecutive patients with SARS-CoV-2 infection confirmed through reverse transcriptase-polymerase chain reaction (RT-PCR, Life Technologies, Carlsbad, CA, USA) from March 16 through March 27 ...
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bioRxiv - Molecular Biology 2021Quote: 2 × 20 μg of each of the three different SARS-CoV-2 spike proteins were mixed with 4x NuPAGE™ LDS sample buffer (Thermo Fisher Scientific) and up to 10 mM dithiothreitol (DTT ...
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bioRxiv - Immunology 2022Quote: ... a TMPRSS2 plasmid and S plasmids from SARS-CoV-2 variants into 293T cells using Lipofectamine 3000 transfection reagent (L3000-001, ThermoFisher Scientific, Asheville, NC) 39,40 ...
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bioRxiv - Immunology 2023Quote: Open-reading frames coding for either Nefmut alone or fused with SARS-CoV-2 N protein were cloned into pVAX1 plasmid (Thermo Fisher, Waltham, MA) as previously described [20] ...
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bioRxiv - Microbiology 2023Quote: ... The ChAd-SARS-CoV-2-BA.5-S genome was rescued following transfection of T-REx™-293 Cell Line (Invitrogen, R710-07). Replication-incompetent ChAd- SARS-CoV-2-BA.5-S ...
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bioRxiv - Microbiology 2023Quote: ... Four microliters RNA was used for real-time RT-qPCR to detect and quantify N gene of SARS- CoV-2 using TaqMan™ RNA-to-CT 1-Step Kit (Thermo Fisher Scientific) as described (73 ...
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bioRxiv - Neuroscience 2023Quote: ... To authenticate SARS-CoV-2 isolates used in the study viral RNA was extracted from stocks using TRIzol LS reagent (Thermo Fisher Scientific, USA) and cDNA was prepared with Protoscript II first-strand cDNA synthesis kit as per manufacturer’s protocol (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... effector cells were stained with rabbit anti-SARS-CoV-2 S S1/S2 polyclonal antibody (Thermo Fisher Scientific, Cat# PA5-112048, 1:100). Normal rabbit IgG (Southern Biotech ...
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bioRxiv - Molecular Biology 2022Quote: RNA was isolated from both SARS-CoV-2 infected (48h post-infection) and non-infected (mock) Vero cells using TRIzol reagent (Thermo Fisher Scientific, 15596026) and Direct-zol RNA Miniprep (ZYMO research ...
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bioRxiv - Microbiology 2022Quote: ... effector cells were stained with rabbit anti-SARS-CoV-2 S S1/S2 polyclonal antibody (Thermo Fisher Scientific, Cat# PA5-112048, 1:100). Normal rabbit IgG (Southern Biotech ...
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bioRxiv - Microbiology 2023Quote: ... effector cells were stained with rabbit anti-SARS-CoV-2 S S1/S2 polyclonal antibody (Thermo Fisher Scientific, Cat# PA5-112048, 1:100). Normal rabbit IgG (Southern Biotech ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were probed with an anti-SARS-CoV-2 spike protein S1/S2 polyclonal antibody (1:1,000, Thermo Fisher Scientific PA5-112048), and an anti-p24 monoclonal antibody (1:1,000) ...
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bioRxiv - Microbiology 2023Quote: ... The following custom TaqMan gene expression assay IDs were used to assess the expression of RSV N and SARS-CoV-2 N genes: ARU66XH and APNKYWD (Applied Biosystems, CA, USA). A no template control was included in each run ...
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bioRxiv - Immunology 2023Quote: ... The ChAd-SARS-CoV-2-BA.5-S genome was rescued following transfection of T-REx™-293 Cell Line (Invitrogen, R710-07). Replication-incompetent ChAd-SARS-CoV-2-BA.5-S ...
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bioRxiv - Microbiology 2023Quote: ... effector cells were stained with rabbit anti-SARS-CoV-2 S S1/S2 polyclonal antibody (Thermo Fisher Scientific, Cat# PA5-112048, 1:100). Normal rabbit IgG (Southern Biotech ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting cDNA was used to quantify the SARS-CoV-2 RNA levels by real-time quantitative PCR using Power SYBR green PCR master mix (Applied Biosystems, Waltham, MA). Average values from duplicates of each sample were used to calculate the viral RNA level relative to the GAPDH gene and presented as 2−ΔCT ...
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bioRxiv - Immunology 2023Quote: Virus samples were serially diluted (10-fold starting at 1:10 initial dilution) in serum-free DMEM (recRSV-mKate and MA-SARS-CoV-2) and supplemented with 0.5% Trypsin (Gibco, recA/California/07/2009). Serial dilutions were added to cells seeded in 96-well plate at 1×104 cells per well ...
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bioRxiv - Cancer Biology 2023Quote: ... with 2% HI FBS (Gibco) and 0.4% 0.5M EDTA (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... and SARS-CoV S protein were confirmed by transfection with the Turbofect transfection reagent (ThermoFisher) followed by radiolabeling and immunoprecipitation analysis using a mouse monoclonal antibody directed against the HA-tag (Thermo-Fisher ...
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bioRxiv - Synthetic Biology 2019Quote: ... Dual immunogold labeling was conducted with 6x-His Tag Monoclonal Antibody (Invitrogen) and the HA Tag Polyclonal Antibody as primary antibodies and an anti-mouse IgG-gold (40 nm ...