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Citations for Thermo Fisher :
101 - 150 of 10000+ citations for Puumala Virus Glycoprotein 2 Gc Human Heterodimeric Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... which is composed of two ACE2 ectodomains linked to the Fc portion of the human IgG (Anand et al., 2020).Alexa Fluor-647-conjugated goat anti-human Abs (Invitrogen) were used as secondary antibodies to detect ACE2-Fc and plasma binding in flow cytometry experiments.
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bioRxiv - Developmental Biology 2022Quote: ... 30 µg of pCAGGS SARS CoV-2 S Glycoprotein expression vector DNA (NR52310, BEI, USA) in a total of 10 mL of Opti-MEM media (Invitrogen, USA). One day after transfection the media was removed ...
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bioRxiv - Immunology 2023Quote: ... HIV reporter pseudoviruses expressing SARS-CoV-2 S glycoprotein and carrying the luciferase gene were produced in Expi293F cells (ThermoFisher Scientific) by co-transfection of the pNL4-3 ...
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bioRxiv - Microbiology 2019Quote: ... and the carbon isotope composition was measured via GC-IRMS on a Thermo Finnigan Trace GC connected with a DELTA V Plus IRMS system via GC Isolink (Thermo Scientific, Bremen Germany). The detailed chromatographic and mass spectrometric parameters were described by Kellermann et al ...
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bioRxiv - Synthetic Biology 2020Quote: The dodecane overlay was analyzed by GC-MS using Trace 1300 GC (ThermoFisher Scientific), equipped with TG-SQC column (15 m × 0.25 mm × 0.25 μm) ...
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bioRxiv - Immunology 2019Quote: ... and the ELISPOT plate was incubated with biotinylated goat anti-human IgG Fc (Invitrogen), followed by HRP-conjugated avidin (Vector Laboratories ...
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bioRxiv - Cancer Biology 2020Quote: ... Then the cells were stained with AlexFuor488-conjugated goat anti human Fc (Life technologies) at RT for 30 min and analyzed by flow cytometry ...
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bioRxiv - Immunology 2021Quote: ... Horseradish peroxidase (HRP)-conjugated antibody specific for the Fc region of human IgG (Invitrogen) was used as secondary antibody to detect antibody binding in ELISA experiments.
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bioRxiv - Microbiology 2021Quote: ... 100 μL of HRP-labelled anti-human IgG Fc antibody (Catalog# 31413, Thermo Fisher) was added to each well and incubated for 1 hour ...
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bioRxiv - Bioengineering 2021Quote: ... nanovials were modified with biotinylated goat anti-human IgG Fc antibody (Thermo Fisher Scientific). Samples were resuspended in DMEM and seeded in a well plate ...
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bioRxiv - Microbiology 2022Quote: ... Horseradish peroxidase (HRP)-conjugated Abs specific for the Fc region of human IgG (Invitrogen) were used as secondary antibodies to detect antibody binding in ELISA experiments ...
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bioRxiv - Immunology 2020Quote: ... Rabbit anti-Human IgG Fc HRP Secondary Antibody (ThermoFisher Scientific Catalog #31423, 1:10,000), and Rabbit anti-Human IgG F(ab’)2 HRP Secondary Antibody (ThermoFisher Scientific Catalog #31482 ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.25 µL Goat anti-Human IgG Fc PE conjugate (ThermoFisher #12-4998-82). Protease susceptibility measurements were performed in biological replicates (n = 2 ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.25 µL Goat anti-Human IgG Fc PE conjugate (ThermoFisher #12-4998-82). Titrations were performed in biological replicates (cultures from independent colonies grown on separate days ...
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bioRxiv - Immunology 2020Quote: ... followed by addition to the Fc-chimeric human ACE2-coated MaxiSORP ELISA plate (Nunc) for an hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: Human JAGGED1-Fc (Martin et al., 2023) was transfected into Expi293F cells (ThermoFisher, A14527) using FectroPro (Polyplus ...
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bioRxiv - Immunology 2024Quote: ... Anti-Human Fc chip was regenerated to baseline using IgG Elution Buffer (Thermo Scientific) and the process was repeated for all humAbs in the panel ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 2% heat-inactivated FCS and 15 mM HEPES (Gibco). Intestines were cut open longitudinally ...
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bioRxiv - Immunology 2023Quote: ... and 2% v/v Fc receptor binding inhibitor (Thermo Fisher Scientific)) ...
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bioRxiv - Cancer Biology 2023Quote: ... containing either 10 or 20% FCS (Bodinco) and 2% PSF (Gibco) and were passaged twice a week ...
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bioRxiv - Molecular Biology 2023Quote: ... Med GC (Thermo Fisher Scientific) was used as a negative control.
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bioRxiv - Microbiology 2020Quote: ... once and then 100 μl of 1:300 diluted secondary antibody (ThermoFisher Cat # A-21091 for Fc-tag and ThermoFisher Cat # MA1-21315-647 for polyhistidine-tag ...
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bioRxiv - Cell Biology 2023Quote: ... ALFA-FAM134s were targeted directly using an anti-ALFA nanobody carrying a 5xR1 docking strand (5-TCCTCCTCCTCCTCCTCCT-3’) (Massive-TAG-Q Anti-ALFA Custom, Massive Photonics, GmbH) which was diluted in permeabilization/blocking buffer (10% FCS (Gibco), 0.1% saponin (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... The monoclonal HA-epitope tag antibody 2-2.2.14 (Invitrogen, catalog no. 26183) was used for detection of hemagglutinin-tagged proteins (1:5000 dilution) ...
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bioRxiv - Cancer Biology 2020Quote: ... Stealth RNAiTM siRNAs Negative Control, Med GC and human Il11, NM_000641.3 (HSS179893, HSS179894, HSS179895) were purchased from Thermo Fisher Scientific.
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bioRxiv - Immunology 2022Quote: ... Undigested IgG and Fc fragments were removed by a 2 h incubation with CaptureSelect IgG-Fc resin (Thermo Fisher Scientific). Resin was spun down and the supernatant run on a Superdex 200 increase 10/300 GL column (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... the metabolic intermediates of naphthalene biodegradation were analyzed using the GC-MS system (GC Trace GC Ultra, MS-Polarisq, Thermo Scientific India Pvt. Ltd) equipped with a capillary column (TR-WaxMS ...
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bioRxiv - Microbiology 2024Quote: ... IL-2 was measured by human IL-2 ELISA (Invitrogen) using the manufacturer instructions ...
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bioRxiv - Immunology 2020Quote: ... at a concentration to give 100 to 250 foci per well in the virus only control wells was mixed 50:50 in 1% FCS MEM with 1 x antibiotic/antimycotic (Gibco, 15240-062) with serum doubling dilutions from 1:20 to 1:640 (or higher dependent on antibody levels ...
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bioRxiv - Microbiology 2021Quote: ... Permeabilized cells were probed with a monoclonal antibody against the HA-tag epitope (1:1000; HA Tag mAb 2-2.2.14, Thermo Fisher Scientific, Planegg, Germany) to detect SARS-2-S protein ...
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bioRxiv - Zoology 2023Quote: ... coupled to the GC via a combustion GC-c-III interface (Thermo Fisher Scientific, USA). Identification of peaks of the FAME chromatogram were based on equivalent chain length (ECL ...
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bioRxiv - Microbiology 2022Quote: ... 1:500 dilution) (goat anti-human IgG Fc Alexa 488 conjugated, Thermo Fisher, Cat #: H10120) (goat anti-human IgG (H + L ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 10% v/v (FCS) and 2 mM L-glutamine (Gibco). Where indicated ...
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bioRxiv - Immunology 2023Quote: ... Samples were stained in FACS buffer (PBS containing 2% FCS (Thermo Fisher) and 2 mM EDTA (Sigma-Aldrich) ...
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bioRxiv - Immunology 2024Quote: ... The cells were then washed in PBS and stained with a mix of secondary antibodies anti-human IgG-Fc-AF647 (1:600) and anti-human IgA-Alpha-Chain-AF488 (1:200) (Thermo Fisher Scientific) for 30 min at 4°C ...
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bioRxiv - Genomics 2019Quote: ... with Human Melanocyte Growth Supplement-2 (Invitrogen). For lentivirus production ...
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bioRxiv - Cancer Biology 2022Quote: ... A Stealth RNAi™ siRNA Negative Control Med GC Duplex #2 (siNC, Cat. No. 12935112, Thermofisher Scientific) was used as negative control siRNA ...
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bioRxiv - Cell Biology 2019Quote: ... Negative Control Medium GC Duplex #2 and siRNA interference for Piezo1 (# AM16708, Assay ID:138387, Thermo Fisher) and Cav-1 (# AM16708 ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 μl of Dynabeads™ His-Tag Isolation and Pulldown beads (ThermoFisher 10104D) for each CFPS reaction were equilibrated twice in equal volumes of wash buffer before addition to the purified reactions to remove the Ulp-protease and incubated with spinning at 4 °C for at least 30 minutes ...
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bioRxiv - Immunology 2019Quote: ... The full length and truncation sequences were transferred into a Gateway compatible destination vector that includes an N terminal triple-epitope tag (S protein tag, FLAG epitope tag and Streptavidin-binding peptide tag) using the LR clonase enzyme kit (ThermoFisher). Plasmids were transfected into 293T cells using FuGENE transfection reagent (Promega ...
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bioRxiv - Microbiology 2019Quote: ... Individual lipid compounds were quantified and identified by gas chromatography with flame ionization detection (GC-FID) and GC-mass spectrometry (GC-MS, Thermo Scientific DSQ II Dual Stage Quadrupole), respectively ...
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bioRxiv - Plant Biology 2020Quote: ... and quantified by GC-MS on a Trace GC/Trace DSQ instrument (Thermo Scientific, Dreieich, Germany) in chemical ionization mode exactly as described previously43.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... GC-MS analysis was conducted using Trace GC Ultra Gas Chromatograph (Thermo Fisher Scientific, Milan, Italy) connected to a DSQ II single quadrupole mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: Cultured SARS-CoV-2 was used to prepare virus stocks in minimum essential medium (MEM 2%) (Gibco, Thermo Fisher Scientific) containing 2% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: Cultured SARS-CoV-2 was used to prepare virus stocks in minimum essential medium (MEM 2%) (Gibco, Thermo Fisher Scientific) containing 2% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... GC-grade Tributylamine 99% (ACROS ORGANICS), LC/MS grade acetic acid Optima (Fisher Chemical) ...
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bioRxiv - Cancer Biology 2021Quote: ... GC-grade Tributylamine 99% (ACROS ORGANICS), LC/MS grade acetic acid Optima (Fisher Chemical) ...
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bioRxiv - Cancer Biology 2020Quote: ... GC-grade Tributylamine 99% (ACROS ORGANICS), LC/MS grade acetic acid Optima (Fisher Chemical) ...
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bioRxiv - Neuroscience 2021Quote: ... and GC enhancer (Thermo Fisher Scientific). Cycling conditions were 10 min at 95°C ...
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bioRxiv - Genetics 2023Quote: ... AccuPrime GC-Rich DNA Polymerase (Invitrogen) was used to amplify a region containing the CAG repeat (35 cycles) ...