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101 - 150 of 4555 citations for Dnp pro leu gly cys me his ala D arg nh2 since 2020

• Zebrafish and cellular models of SELENON-Related Myopathy exhibit novel embryonic and metabolic phenotypes

  Pamela Barraza-Flores, et al., bioRxiv - Molecular Biology 2024

  Quote: ... following manufacturer’s instructions using 15 μg of total protein detected with rabbit anti-DNP antibodies (Invitrogen) at 1:1,000 and HRP-conjugated AffiniPure Goat Anti-Rabbit IgG (1:10,000) ...
• Functional Integration of 3D-Printed Cerebral Cortical Tissue into a Brain Lesion

  Yongcheng Jin, et al., bioRxiv - Bioengineering 2022

  Quote: ... The resuspended DNPs were transferred into cryopreservation vials and slow cooled in Freezing Containers (Thermo Scientific) at −80°C overnight ...
• Endothelial-secreted Endocan protein acts as a PDGFR alpha ligand and regulates vascularity, radioresistance, and regional phenotype in glioblastoma

  Soniya Bastola, et al., bioRxiv - Cancer Biology 2023

  Quote: ... recombinant His-tagged Endocan (R&D) was immobilized on 50 μl of HisPur Ni-NTA Magnetic Beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
• Endothelial-secreted Endocan protein acts as a PDGFR alpha ligand and regulates vascularity, radioresistance, and regional phenotype in glioblastoma

  Soniya Bastola, et al., bioRxiv - Cancer Biology 2023

  Quote: Recombinant His-tagged Endocan (R&D) was immobilized on 50 μl of HisPur Ni-NTA Magnetic Beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
• RNA-seq of Human T-Cells After Hematopoietic Stem Cell Transplantation Identifies Linc00402 as a Novel Regulator of T-Cell Alloimmunity

  Daniel Peltier, et al., bioRxiv - Immunology 2020

  Quote: Ten micrograms of total RNA (RIN > 8 for all samples) was mixed with Gly Loading Dye (Ambion) and heated to 48°C for 1 hour ...
• A dynamic balance between neuronal death and clearance after acute brain injury

  Trevor Balena, et al., bioRxiv - Neuroscience 2023

  Quote: ... PO-PRO-1 iodide (435/355) (1 mM Solution in DMSO) (PO-PRO; ThermoFisher) was incubated in slices for a minimum of 3 hrs at a concentration of 5 μM (final DMSO concentration = 0.5%) ...
• Disruption of Epithelial Integrity Drives Mesendoderm Differentiation in Human Induced Pluripotent Stem Cells by Enabling BMP and ACTIVIN Sensing

  Diane Rattier, et al., bioRxiv - Cell Biology 2021

  Quote: ... ME differentiation was induced by threating PS differentiated cultures for 2 days with BMP4 (R&D, ref. 314-BP, 10 ng/mL) in ME differentiation medium (RPMI 1640, 2% B27 without Insulin (Gibco, ref. A1895601), 1% Penicillin/Streptomycin ...
• Smart3-ATAC: a highly sensitive method for joint accessibility and full-length transcriptome analysis in single cells

  Huaitao Cheng, et al., bioRxiv - Systems Biology 2021

  Quote: ... Tn5 underwent a His-tag (Dynabeads His-Tag Isolation & Pulldown, Invitrogen, 10103D) clean-up according to the manufacturer’s protocol to remove unbound oligos before using.
• Canonical Wnt signaling promotes formation of somatic permeability barrier for proper germ cell differentiation

  Ting-An Chen, et al., bioRxiv - Developmental Biology 2022

  Quote: ... or TO-PRO-3 (Invitrogen) for 10 min at RT or O/N at 4°C ...
• Regulated extracellular matrix trafficking shapes cell growth during cartilage morphogenesis

  Daniel S. Levic, et al., bioRxiv - Developmental Biology 2022

  Quote: ... To-Pro-3 (Molecular Probes) or 4’,6-diamidino-2-phenylindole (DAPI ...
• Single-domain antibodies for targeting, detection and in vivo imaging of human CD4⁺ cells

  Bjoern Traenkle, et al., bioRxiv - Immunology 2021

  Quote: ... YO-PRO-1 (Invitrogen, USA) was used for nuclear staining ...
• Protective function of ex vivo expanded CD8 T cells in a mouse model of adoptive therapy for cytomegalovirus infection depends on integrin beta 1 but not CXCR3, CTLA4, or PD-1 expression

  Xiaokun Liu, et al., bioRxiv - Immunology 2024

  Quote: ... or TO-PRO-3TM (Invitrogen). The immunofluorescent photomicrographs were acquired with the Axiovert 200M inverted microscope (Carl Zeiss ...
• Lung cancer-intrinsic SOX2 expression mediates resistance to checkpoint blockade therapy by inducing Treg-dependent CD8+ T cell exclusion

  Elen Torres-Mejia, et al., bioRxiv - Immunology 2023

  Quote: ... or TO-PRO-3 (ThermoFisher). Samples were mounted using ProLong Diamond Antifade Mountant and curated for 24 hours before imaging ...
• A dual role in virion attachment and entry makes the human cytomegalovirus gHgLgO trimer the central player in virion infectivity

  Lena Thiessen, et al., bioRxiv - Microbiology 2024

  Quote: ... in Opti-PRO medium (Gibco) supplemented with 25 mM HEPES (Gibco) ...
• Secreted SARS-CoV-2 ORF8 modulates the cytokine expression profile of human macrophages

  Nisha Kriplani, et al., bioRxiv - Microbiology 2021

  Quote: ... 10% HI FBS (Gibco), 1X ultraglutamine (Lonza) ...
• Vaccinia E5 is a major inhibitor of the DNA sensor cGAS

  Ning Yang, et al., bioRxiv - Microbiology 2021

  Quote: ... His (ThermoFisher, MA1-21315), FLAG (Sigma ...
• Neutralization of viruses with European, South African, and United States SARS-CoV-2 variant spike proteins by convalescent sera and BNT162b2 mRNA vaccine-elicited antibodies

  Takuya Tada, et al., bioRxiv - Microbiology 2021

  Quote: ... anti-His mAb (Invitrogen) and anti-GAPDH mAb (Life Technologies ...
• Photoinactivation of catalase sensitizes Candida albicans and Candida auris to ROS-producing agents and immune cells

  Pu-Ting Dong, et al., bioRxiv - Microbiology 2021

  Quote: ... HI FBS (2273356P, Gibco). HEPES (54457 ...
• A novel antigenic site spanning domains I and III of the Zika virus envelope glycoprotein is the target of strongly neutralizing human monoclonal antibodies

  Stephen Graham, et al., bioRxiv - Immunology 2020

  Quote: ... or anti-His (Invitrogen) for recombinant ZVrecE80 antigen in 0.1M carbonate buffer pH 9.6 ...
• Ultra-pure isolation of low density neutrophils casts doubt on their exceptionality in health and disease

  Gareth R Hardisty, et al., bioRxiv - Immunology 2020

  Quote: ... + 10% HI-FCS (Gibco) + 1% Peniciliin/Streptomycin (100x ...
• Survivin Mediates Mitotic Onset in HeLa Cells Through Activation of the Cdk1-Cdc25B Axis

  Pedro M. Cánovas, bioRxiv - Cancer Biology 2023

  Quote: ... and His-tag (Invitrogen) were used ...
• A Zur-mediated transcriptional regulation of the zinc export system

  Verena Ducret, et al., bioRxiv - Microbiology 2022

  Quote: ... anti-penta HIS (Invitrogen) and anti-HSP70 (26 ...
• STING-Activating Polymer-Drug Conjugates for Cancer Immunotherapy

  Taylor L. Sheehy, et al., bioRxiv - Bioengineering 2024

  Quote: ... 10% HI-FBS (Gibco), 100 U ml−1 penicillin/100 μg ml−1 streptomycin (Gibco) ...
• Conserved regulatory motifs in the juxtamembrane domain and kinase N-lobe revealed through deep mutational scanning of the MET receptor tyrosine kinase domain

  Gabriella O. Estevam, et al., bioRxiv - Molecular Biology 2024

  Quote: ... 10% HI-FBS (Gibco), 1% penicillin/streptomycin (Gibco) ...
• Conserved regulatory motifs in the juxtamembrane domain and kinase N-lobe revealed through deep mutational scanning of the MET receptor tyrosine kinase domain

  Gabriella O. Estevam, et al., bioRxiv - Molecular Biology 2024

  Quote: ... 10% HI-FBS (Gibco), 1% penicillin/streptomycin (Gibco) ...
• Conserved regulatory motifs in the juxtamembrane domain and kinase N-lobe revealed through deep mutational scanning of the MET receptor tyrosine kinase domain

  Gabriella O. Estevam, et al., bioRxiv - Molecular Biology 2024

  Quote: ... 10% HI-FBS (Gibco), 1% penicillin/streptomycin (Gibco) ...
• Mapping kinase domain resistance mechanisms for the MET receptor tyrosine kinase via deep mutational scanning

  Gabriella O. Estevam, et al., bioRxiv - Cancer Biology 2024

  Quote: ... 10% HI-FBS (Gibco), 1% penicillin/streptomycin (Gibco) ...
• Mapping kinase domain resistance mechanisms for the MET receptor tyrosine kinase via deep mutational scanning

  Gabriella O. Estevam, et al., bioRxiv - Cancer Biology 2024

  Quote: ... 10% HI-FBS (Gibco), 1% penicillin/streptomycin (Gibco) ...
• Pharmacologic hyperstabilisation of the HIV-1 capsid lattice induces capsid failure

  K. M. Rifat Faysal, et al., bioRxiv - Biophysics 2023

  Quote: CPSF6p-Cys was labelled with Alexa-Fluor 568-C5-maleimide (Thermo Fisher Scientific) added at an equimolar ratio in HEPES buffer pH 8 ...
• Changes in Protein N-Glycosylation Regulation Occur in the Human Parkinsonian Brain in a Region-Specific Manner

  Ana Lúcia Rebelo, et al., bioRxiv - Neuroscience 2023

  Quote: ... Cy®5 goat anti-mouse IgG (H+L) (Invitrogen, A10524, 1:500)) in TBS ...
• Thrombolysis exacerbates cerebrovascular injury after ischemic stroke via a VEGF-B dependent effect on adipose lipolysis

  Ingrid Nilsson, et al., bioRxiv - Neuroscience 2024

  Quote: ... a Cy-3 conjugated donkey anti-guinea pig antibody was used (Molecular Probes). Fab-specific secondary antibodies were used for detection of VEGFR1 ...
• Tdrd3 regulates the progression of meiosis II through translational control of Emi2 mRNA in mouse oocytes

  Natsumi Takei, et al., bioRxiv - Developmental Biology 2021

  Quote: ... the samples were incubated overnight with anti-DNP-Alexa Fluor 488 antibody (1:500 dilution; Molecular Probes) after reaction with tyramide DNP ...
• Analysis of coagulation factor IX in bioreactor cell culture medium predicts yield and quality of the purified product

  Lucia F. Zacchi, et al., bioRxiv - Biochemistry 2020

  Quote: ... in 1x MES running buffer (Invitrogen), under non-reducing conditions ...
• Human pluripotent stem cell modeling of tuberous sclerosis complex reveals lineage-specific therapeutic vulnerabilities

  Sean P. Delaney, et al., bioRxiv - Cancer Biology 2020

  Quote: ... 500uL β-ME (Thermo Fisher, #21985023), and 500µL Gentimicin (Wisent ...
• Grad-seq shines light on unrecognized RNA and protein complexes in the model bacterium Escherichia coli

  Jens Hör, et al., bioRxiv - Microbiology 2020

  Quote: ... using 1x MES buffer (Thermo Fisher). The gel was stained with SimplyBlue Coomassie (Thermo Fisher ...
• Live cell imaging of the hyperthermophilic archaeon Sulfolobus acidocaldarius identifies complementary roles for two ESCRTIII homologues in ensuring a robust and symmetric cell division

  Andre Arashiro Pulschen, et al., bioRxiv - Microbiology 2020

  Quote: ... using MES SDS running buffer (Invitrogen). Proteins were transferred to a 0.45μm nitrocellulose membrane ...
• Biomolecular Tau condensation is linked to Tau accumulation at the nuclear envelope

  Janine Hochmair, et al., bioRxiv - Molecular Biology 2022

  Quote: ... MES-buffer containing 0.1 % SDS (Invitrogen) was used as running buffer in both SDS-PAGE and Native-PAGE and 0.5 µg Tau were loaded per well ...
• Use of a novel knock-in allele of Pkd1 identifies nicotinamide nucleotide dehydrogenase as a mitochondrial binding partner of polycystin-1

  Cheng-Chao Lin, et al., bioRxiv - Genetics 2022

  Quote: ... run in MES buffer (Invitrogen, NP0002) at 200V for 45 min. ...
• Melanogenic Activity Facilitates Dendritic Cell Maturation via FMOD

  Marianna Halasi, Irit Adini, bioRxiv - Immunology 2022

  Quote: ... 0.5% 2-mercaptoethanol (2-ME) (GIBCO) and 1% HEPES (GIBCO) ...
• SARS-CoV-2 envelope-protein corruption of homeostatic signaling mechanisms in mammalian cells

  Tobias Schulze, et al., bioRxiv - Pathology 2021

  Quote: ... in MES running buffer (ThermoFisher Scientific) at constant 150 V until the blue loading dye front reached the end of the gel ...
• Autophagy in T cells from aged donors is maintained by spermidine, and correlates with function and vaccine responses

  Ghada Alsaleh, et al., bioRxiv - Physiology 2020

  Quote: ... with MES running buffer (Thermo Fisher) was used ...
• Characterisation of the bovine C-type lectin receptor Mincle and potential evidence for an endogenous ligand¹

  Angela Holder, et al., bioRxiv - Immunology 2023

  Quote: ... using MES (immunogen) (Thermo Fisher Scientific) or MOPS (boPBMCs ...
• Translation is a key determinant controlling the fate of cytoplasmic long non-coding RNAs

  Sara Andjus, et al., bioRxiv - Molecular Biology 2023

  Quote: ... in MES buffer (Invitrogen; cat# NP0002), then the region of the gel encompassing the small peptides fraction (1-10 kDa ...
• Fitness Tradeoffs of Multidrug Efflux Pumps in Escherichia coli K-12 in Acid or Base, and with Aromatic Phytochemicals

  Yangyang Liu, et al., bioRxiv - Microbiology 2023

  Quote: ... MES and methyl-salicylate (ACROS Organics); sodium ferulate (Selleck Chemical) ...
• Tau seeding and spreading in vivo is supported by both AD-derived fibrillar and oligomeric tau

  Anastasie Mate de Gerando, et al., bioRxiv - Neuroscience 2023

  Quote: ... with NuPAGE MES running buffer (ThermoFisher). Anti-total tau antibody (1 ...
• Streptavidin-drug conjugates streamline optimization of antibody-based conditioning for hematopoietic stem cell transplantation

  Aditya R. Yelamali, et al., bioRxiv - Immunology 2024

  Quote: ... and 55 μM 2-ME (Gibco)) ...
• Streptococcus agalactiae and Escherichia coli Induce Distinct Effector γδ T Cell Responses During Neonatal Sepsis and Neuroinflammation

  Lila T. Witt, et al., bioRxiv - Immunology 2023

  Quote: ... and 0.55 mM 2-ME (Gibco). 1X protein transport inhibitor (Fisher Healthcare ...
• Development of a cellular reporter assay to measure activity of MutSβ, a therapeutic target for Huntington’s disease

  Jian An, et al., bioRxiv - Neuroscience 2023

  Quote: ... and MES running buffer (ThermoFisher, NP0002). Proteins were transferred to nitrocellulose membranes (Bio-Rad ...
• Glutarate regulates T cell function and metabolism

  Eleanor Minogue, et al., bioRxiv - Immunology 2022

  Quote: ... 55 μM 2-ME (21985023, Gibco) and 10U/mL IL-2 (11147528001 ...
• Anti-nuclear antibody (ANA) detection in culture supernatant of cloned B cells

  Shabirul Haque, et al., bioRxiv - Immunology 2023

  Quote: ... and 55 μM 2-ME (GIBCO).