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Citations for Thermo Fisher :
1 - 50 of 4555 citations for Dnp pro leu gly cys me his ala D arg nh2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: The HHH peptide (Cys-biotin-PEG2-Gly-His-His-His-Gly(COOH)) was purchased from Thermo Fisher. It was 84% pure as determined by mass spectrometry ...
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bioRxiv - Microbiology 2020Quote: ... fused to a Gly-Ser-Gly linker and a Myc epitope tag into the EcoRV site of pEF1 V5 His C (Invitrogen) using NEB HiFi Assembly Mix (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... The enzymatic activity of Ddl was also assayed by measuring the release of inorganic phosphate in the reaction (similar to the D-ala-D-ala dipeptide formation assay) with EnzChek phosphate assay kit (Life Technologies). 10 μl of the reaction product was added to 790 μl of standard reaction mixture containing 230 μl dH2O ...
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bioRxiv - Cell Biology 2022Quote: ... SILAC media was prepared by supplementing high glucose DMEM minus Arg/Lys/Leu/Met (AthenaES) with 10% dialyzed FBS (ThermoFisher) and the appropriate amino acids ...
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bioRxiv - Cell Biology 2022Quote: ... SILAC media was prepared by supplementing high glucose DMEM minus Arg/Lys/Leu/Met (AthenaES) with 10% dialyzed FBS (ThermoFisher) and the appropriate amino acids ...
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bioRxiv - Cell Biology 2022Quote: ... DNP conjugated bovine serum albumin (DNP-BSA) was from Invitrogen (USA). Constructs of the following plasmids were kind gifts ...
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bioRxiv - Biochemistry 2021Quote: ... -Arg (Thermo Fisher Scientific), Phalloidin-Alexa 594 (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... Ala-Gln (Ultra-Glutamine) (Gibco), 5% heat-inactivated horse serum (Lonza) ...
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bioRxiv - Immunology 2023Quote: ... which encodes for a C-terminal C-Direct tag containing a free thiol (cysteine) and an EPEA (Glu, Pro, Glu, Ala) purification tag (C-tag, Thermo Fisher Scientific). Vectors were transformed into yeast cells (Saccharomyces cerevisiae strain VWK18 (51)) ...
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bioRxiv - Immunology 2021Quote: ... 1% Arg/Asp/Folic acid (Gibco), 1% HEPES (Gibco) ...
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bioRxiv - Neuroscience 2022Quote: ... Arg-free Neurobasal (Gibco, Life Technologies), 10 mL B-27 (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... Arg-free Neurobasal (Gibco, Life Technologies), 10 mL B-27 (Gibco ...
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bioRxiv - Molecular Biology 2020Quote: ... in 1.5ml of SD/-Leu/-His liquid medium (plus 3 mM 3-AT) were incubated in a 12-well microplate (Thermo Scientific) at 30°C for 96 hours (h) ...
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bioRxiv - Plant Biology 2024Quote: ... were selected by growth on SD-Leu-His plates that contained 20 mM 3-amino-1,2,4-triazole (3AT; Fisher Scientific, MA, USA) as described (Jahan et al. ...
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bioRxiv - Cancer Biology 2024Quote: ALA powder (Thermo Fisher Scientific; Waltham, MA) was stored at -20℃ ...
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bioRxiv - Genomics 2022Quote: ... and DNP-labeled probes were detected with 1:500 diluted DNP rabbit antibody (ThermoFisher, Catalog no. 04-8300) and 1:150 diluted Anti-rabbit Alexa Fluor 546-conjugated goat antibody (ThermoFisher ...
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bioRxiv - Developmental Biology 2022Quote: ... and/or Rabbit anti-DNP (Thermo Fisher) or αDIG-AP ...
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bioRxiv - Physiology 2020Quote: ... such as trypsin and chymotrypsin were determined in pancreatic tissue homogenates using fluorescent substrates as previously reported (34): Trypsin substrate (R110-(CBZ-Ile-Pro-Arg)2 was purchased from Life Technologies (Carlsbad, CA, USA); Chymotrypsin (Suc-AAPF-AMC ...
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bioRxiv - Biochemistry 2024Quote: ... and Gly-AMC (Thermo Scientific Chemicals, J65592.MA). Kinetic parameters for tripeptides LLL and LPL were obtained in the same way as described above using tripeptide-AMC conjugates (synthesized by GenScript) ...
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bioRxiv - Cell Biology 2023Quote: ... Cy-2- or Cy-5-conjugated goat anti-mouse secondary antibodies (Thermo Fisher Scientific) were used at a 1:750 dilution in 5% HIGS/PBS ...
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bioRxiv - Biochemistry 2024Quote: ... and NH2-PEG11-Biotin (ThermoFisher, UK, #26136) were mixed in a 1:3 ratio for at least 1 h at 24°C in 1X PBS (pH 7.2) ...
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bioRxiv - Cell Biology 2024Quote: ... and PE-Arg-1 (Invitrogen; 12-3697-82) for 30 minutes at 4°C in dark ...
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bioRxiv - Biophysics 2022Quote: ... SC-Leu + 20mg/ml MPA (Fisher Scientific), SC-Leu + 15 mM Mn (Sigma) ...
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bioRxiv - Genetics 2023Quote: ... SC-Leu + 20mg/ml MPA (Fisher Scientific), SC-Leu + 15 mM Mn (Sigma) ...
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bioRxiv - Bioengineering 2021Quote: ... using Tris-Gly 8-16% gels (Novex, Thermo Fisher) at 225V for 36 minutes stained with Blazin Blue (Goldbio ...
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bioRxiv - Molecular Biology 2022Quote: ... Dm III-2 and Dm III-2 + cys production was confirmed via western blot using 6x His-tag Ab (Invitrogen, Carlsbad, CA, USA). Positive clones were expanded ...
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bioRxiv - Cell Biology 2020Quote: ... Stable transformants of ZP161 expressing rat Pex14 variants tagged with N-terminal hexahistidine (His-Pex14) were isolated by transfection of pcDNAZeo-D/His-RnPEX14 variants (see below) followed by selection with Zeocin (Invitrogen), as described (Okumoto et al. ...
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bioRxiv - Cell Biology 2022Quote: ... DNP (EM 1:6-1:30, #71-3500, Invitrogen), DPP4 (IF/FACS 1:100 ...
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bioRxiv - Biophysics 2023Quote: ... and/or anti-DNP antibodies (Rat monoclonal, Thermo Fisher Scientific ...
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bioRxiv - Pathology 2024Quote: NH2 cartridge HyperSep 500 mg (Thermo Fisher Scientific) were conditioned by adding 2 ml of chloroform:methanol (23:1 volume ...
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bioRxiv - Cancer Biology 2021Quote: ... Arg-1 rabbit polyclonal antibody (Invitrogen cat# PA5-85287) staining was performed at 1:15,000 dilution followed by secondary antibody 570 opal reagent (1:100 dilution ...
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bioRxiv - Immunology 2022Quote: ... and Arg-1 (A1exF5,Invitrogen-ThermoFisher Scientific, Waltham, MA). The viability dye ...
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bioRxiv - Immunology 2022Quote: ... and Arg-1 (A1exF5,Invitrogen-ThermoFisher Scientific, Waltham, MA). The viability dye ...
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bioRxiv - Immunology 2020Quote: ... were then loaded onto 1% agarose NorthernMax-Gly gels (Ambion) and electrophoresed at 135V for 2 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... containing either light Arg/Lys or heavy isotope-labeled Arg (13C6 15N4 L-arginine)/Lys (13C6 15N2 L-lysine) (Thermo Fisher Scientific, respectively) at least six doubling times ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-DNP-KLH antibodies (Molecular Probes, #A6430, 1:200), and biotinylated anti-HA antibodies (BioLegend ...
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bioRxiv - Neuroscience 2020Quote: ... Alexa 633 or Cy-5 (Thermo Fisher). Sections were counterstained with 4’,6-diamidino-2’-phenylindole dihydrochloride (DAPI ...
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bioRxiv - Neuroscience 2024Quote: ... or Goat αMouse Cy-3 (Invitrogen, #A10521) (1:500) ...
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bioRxiv - Immunology 2023Quote: ... quantification and analysis of product size (NorthernMax®-Gly gel, ThermoFisher). For optimising overexpression conditions ...
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bioRxiv - Molecular Biology 2023Quote: ... Experiments were carried out using 8 μL of SETDB1-3TD (residues 196-403, N-terminal His tag) and 20X concentration Sy-pro Orange dye (5000X DMSO stock, ThermoFisher) and 2 uL of ligand for a final concentration of 20 μM of protein and 200 μM of ligand with 1.1% of DMSO ...
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bioRxiv - Immunology 2021Quote: ... mice were woken up to allow any fecal matter to evacuate and then anesthetized again to introduce 100μL of Cy-5-labelled glucose or Cy-5 secondary goat anti-rat antibody (0.1mM diluted in PBS, ThermoFisher A10525) into the colon via a gavage needle enema ...
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bioRxiv - Microbiology 2021Quote: ... cells were starved in Cys-/Met-media (Gibco) for 45 minutes ...
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bioRxiv - Microbiology 2021Quote: ... cells were starved in Cys-/Met-media (Gibco) for 45 minutes ...
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bioRxiv - Microbiology 2021Quote: ... cells were starved in Cys-/Met-media (Gibco) for 45 minutes ...
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bioRxiv - Microbiology 2022Quote: ... blotted and hybridised using reagents from the NorthernMax – Gly Kit (Thermo Fisher) unless otherwise indicated ...
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bioRxiv - Biochemistry 2021Quote: Covalently linked OtUBD resin was made by conjugating Cys-OtUBD or Cys-His6-OtUBD to SulfoLink™ coupling resin (Thermo Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... and detected with Alexa Fluor 488-conjugated anti-DNP antibody (Molecular Probes). The ISH probes are listed in Supplemental Table 1b ...
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bioRxiv - Cancer Biology 2022Quote: ... or 13C6,15N4-Arg and 13C6,15N2-Lys (Thermo Fisher Scientific, Waltham, MA. USA) until the populations went through six passages ...
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bioRxiv - Immunology 2022Quote: ... loaded on a denatured 1% agarose gel in NorthernMax-Gly running buffer (Invitrogen) alongside Millennium RNA Markers (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2023Quote: ... in Cys- and Met-free DMEM (Gibco™ 21013024) medium for 10 ...