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Citations for Thermo Fisher :
1401 - 1450 of 10000+ citations for Recombinant Human FCGRT & B2M Protein His Avi Strep II tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... we transfected one 100 mm plate of DLD1 BRCA2-/- cells at 70% of confluence with 10 µg of a plasmid containing human EGFP-MBP-tagged BRCA2 cDNA (corresponding to accession number NM_000059) using TurboFect (Thermo Fisher Scientific), 48h post-transfection the cells were serial diluted and cultured in media containing 1 mg/ml G418 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... N-terminal FLAG-tagged human MCC (P23508-1) constructs (pCMV2B vector) were transfected into HEK293 cells and selected with G418 (Gibco #10131035). G418-resistant cells were grown ...
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bioRxiv - Molecular Biology 2020Quote: ... StrepII- and MBP-tagged human PUM1 and PUM2 RD and RBD constructs were expressed in E.coli BL21 (DE3) Star cells (Thermo Fisher Scientific). Cells were grown in LB overnight at 37°C ...
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bioRxiv - Immunology 2021Quote: ... HEK293T cells were transfected with plasmid encoding Myc-tagged human NLRP3 using the Lipofectamine™ 3000 transfection kit (L3000015, Thermo fisher). At 24 h after transfection ...
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bioRxiv - Biophysics 2022Quote: ... FLAG-tagged human DNA2 nuclease-deficient (D277A) and helicase-deficient (K654R) mutants were generated by Phusion site-directed mutagenesis (Thermo Fisher) of wild-type DNA2-FLAG (pIF494 ...
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bioRxiv - Neuroscience 2023Quote: ... FLAG-tagged WT or kinase dead (KD, CDKL5 K42R) constructs of human CDKL5 were subcloned into a pT7CFE1-CHis plasmid (Thermo Fisher). A HeLa cell lysate-based Kit (1-Step Human Coupled IVT Kit—DNA ...
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bioRxiv - Cell Biology 2023Quote: Methods used for these assays have been described.28 Briefly, human FLAG-tagged CDKL5 WT or kinase dead (KD, CDKL5 K42R) constructs were subcloned into pT7CFE1-CHis plasmid (Thermo Fisher). These constructs were then used for in vitro translation using a HeLa cell lysate-based Kit (1-Step Human Coupled IVT Kit—DNA ...
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bioRxiv - Immunology 2023Quote: ... monocytes/macrophages (CD3-CD19-NKp46-CD11b+Gr1loLyC6int/hi) and neutrophils (CD3-CD19-NKp46- CD11b+Gr1+LyC6int) were counted using a Countess II Automated Cell Counter (Thermo Fisher) and then combined and processed as one sample per mouse ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with Bovine Pituitary Extract (25 mg) and EGF Human Recombinant (2.5 μg) (Gibco, Life Technologies Limited, USA). This medium was established through previous successes in obtaining cell lines from primary tissue [26] ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with Bovine Pituitary Extract (25 mg) and EGF Human Recombinant (2.5 μg) (Gibco, Life Technologies Limited, USA). This medium was established through previous successes in obtaining cell lines from primary tissue [26] ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant human IgG1 mAbs were produced by co-transfection of Freestyle 293-F suspension cells (Thermo Fisher Scientific) as previously described 57 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... Recombinant human IgG1 mAbs were produced by co-transfection of Freestyle 293-F suspension cells (Thermo Fisher Scientific) as previously described 48 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... supplemented with human recombinant epidermal growth factor (rEGF) and bovine pituitary extract (BPE) plus 2% penicillin-streptomycin (Gibco) at 37°C in 5% CO2 ...
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bioRxiv - Biophysics 2022Quote: ... and 1 μg/ml Recombinant Human Fibroblast growth Factor-basic (FGFb) (Catalog # 13256-029 Gibco, Thermo Fisher Scientific) at 37°C with 5% CO2 ...
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bioRxiv - Biophysics 2022Quote: ... and 1 μg/ml Recombinant Human Fibroblast growth Factor-basic (FGFb) (Catalog # 13256-029 Gibco, Thermo Fisher Scientific) at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2022Quote: ... EGF-Cy3B was generated using NHS-Cy3B (Cytiva, Marlborough, MA) conjugated to recombinant human EGF (Gibco, ThermoFisher Scientific), using 1:1 labeling stoichiometry as described previously 60 ...
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bioRxiv - Cell Biology 2022Quote: ... EGF-Cy3B was generated using NHS-Cy3B (Cytiva, Marlborough, MA) conjugated to recombinant human EGF (Gibco, ThermoFisher Scientific), using 1:1 labeling stoichiometry as described previously 60 ...
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bioRxiv - Cell Biology 2022Quote: ... at 37 °C and 5% CO2 in EMEM (ATCC) supplemented with 0.01 mg/mL human recombinant insulin (Gibco) and 10% (vol/vol ...
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bioRxiv - Immunology 2020Quote: ... were used for T cell activation and expansion in the condition of human recombinant IL-2 (Gibco, PHC0021) or where indicated ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 1% penicillin–streptomycin in 6-well plates coated with truncated recombinant human vitronectin (Thermo Fisher Scientific). The iPSC line was previously assessed for pluripotency and normal karyotype.33 For differentiation ...
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bioRxiv - Neuroscience 2022Quote: ... hiPS cells were maintained in 6-well plates coated with recombinant human vitronectin (VTN-N, Life Technologies, A14700) in Essential 8 medium (Life technologies ...
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bioRxiv - Neuroscience 2023Quote: ... hiPSCs were maintained in six-well plates coated with recombinant human vitronectin (VTN-N, Thermo Fisher Scientific, A14700) in Essential 8 medium (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: Cell culture medium Essential 8 (E8) Flex Medium and recombinant human vitronectin (rhVTN-N) were purchased from Thermofisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 0.055 mM β-mercaptoethanol and 10 ng/ml recombinant human full-length bFGF (All reagents from Gibco, USA)].
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bioRxiv - Neuroscience 2024Quote: ... hiPS cells were cultured in six-well plates coated with recombinant human vitronectin (Life Technologies, catalogue no. A14700) using Essential 8 medium (Life Technologies ...
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bioRxiv - Genomics 2019Quote: ... 500 ng of in situ Hi-C libraries were first mixed with 2.5 μg human Cot-1 DNA (Invitrogen #15279011), 2.5 μg salmon sperm DNA (Invitrogen #15632011) ...
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bioRxiv - Immunology 2021Quote: ... The expression vectors encoding mHVEM (Q39-Q206) fused with human IgG1 and a subsequent hexa-His tag sequences were transfected into Expi293 (Gibco) cells using ExpiFectamine 293 transfection kit (Gibco ...
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bioRxiv - Immunology 2021Quote: ... The DNA sequences encoding a protein biologic composed of mHVEM residues (Q39- Q206) followed by human IgG1 and a subsequent hexa-His tag sequences were cloned into pcDNA 3.3 vector (Life technologies) using In-fusion HD cloning enzyme premix (Clontech) ...
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bioRxiv - Biophysics 2021Quote: Scc2/4 was labeled by mixing the protein in an equimolar ratio with Qdot 705 Strep Conjugate (Thermo Fisher Scientific) and incubating for 10 min on ice in BSA buffer (40 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: ... ADP-ribosylated proteins were separated by 10% SDS-PAGE and detected by using Strep-HRP (Thermo Scientific, Waltham, MA, USA).
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bioRxiv - Cell Biology 2022Quote: ... Biotinylated proteins were captured and purified from the supernatant by Pierce High Capacity NeutrAvidin Agarose (Thermo Fisher Scientific). After washing six times with lysis buffer ...
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bioRxiv - Immunology 2021Quote: ... Biotinylated spike protein was purified using a 40K MWCO 2 mL Pierce Zeba™ desalting column (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2020Quote: ... A small amount of the purified proteins were biotinylated using the EZ-link BMCC-biotin system (ThermoFisher Scientific). ≈1 mg BMCC-biotin was dissolved directly in 100% DMSO to a concentration of 8 mM for labeling ...
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bioRxiv - Neuroscience 2020Quote: ... GAD65/67 and gephyrin proteins were detected using biotinylated goat anti-rabbit and streptavidine Alexa 488 (Molecular Probes; catalog No ...
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bioRxiv - Neuroscience 2021Quote: ... pH 7.2) and surface proteins were biotinylated using 0.5 mg/ml Sulfo-NHS-SS-Biotin (Thermo Fisher Scientific) in PBS for 40 min at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein ligands (Red1288-345 and Iss1) were biotinylated using EZ-Link NHS-PEG4-Biotin (Thermo Fisher Scientific). Biotin-labeled proteins were immobilized on the streptavidin (SA ...
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bioRxiv - Biophysics 2024Quote: ... CALHM2 and CALHM4 proteins were chemically biotinylated using amine-reactive EZ-Link™ NHS-PEG4-Biotin (ThermoFisher Scientific). For this purpose ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested and biotinylated peptides were captured (80 μl Pierce™ Streptavidin UltraLink™ Resin, ThermoFisher Scientific), resin was washed ...
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bioRxiv - Immunology 2023Quote: ... mAbs or ACE2 protein were first biotinylated using the EZ-Link Sulfo-NHS-Biotinylation Kit (Thermo Fisher Scientific) followed by removal of unreacted biotinylation reagents using a Zeba Spin Desalting Column (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: Purified RECQL5 and sfGFP proteins were biotinylated using EZ-Link Sulfo-NHS-LC-Biotinylation Kit (Thermo Scientific #21435) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... and 1% Pen-Strep (Penicillin/ Streptomycin; Gibco), and incubated at 37°C in a 5% CO2 humidified atmosphere ...
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bioRxiv - Cancer Biology 2021Quote: ... 1% Pen Strep RPMI 1640 (GIBCO 11875093). Normal breast epithelial cells HMLE and MCF-10A were cultured in MEGM (Lonza CC-3151 ...
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bioRxiv - Cell Biology 2020Quote: ... Pen-Strep (10000 U/ml) (Thermofisher, 15140122), EGM-2 ™ Endothelial Growth Medium-2 BulletKit™ (Lonza ...
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bioRxiv - Cell Biology 2020Quote: ... and 0.1 mg/ml Pen-strep (Gibco) was freshly prepared ...
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bioRxiv - Cell Biology 2020Quote: ... and 1% Pen/Strep (Invitrogen #15070-063). MDA-MB-231 cells were received at passage 15 were grown in DMEM (Invitrogen #11965-092) ...
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High resolution, serial imaging of early mouse and human liver bud morphogenesis in three dimensionsbioRxiv - Developmental Biology 2021Quote: ... Pen-Strep (10000 U/ml) (Thermofisher, 15140122), 0.05% Trypsin-EDTA (Cat ...
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bioRxiv - Developmental Biology 2021Quote: ... 1% Penicillin-Streptomycin (Pen-Strep) (Gibco 15140148). Passage 4 cells were used in all experiments ...
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bioRxiv - Developmental Biology 2021Quote: ... 1:100 pen-strep (Gibco 15140-122), 1:100 GlutaMax (Gibco 35050-061) ...
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bioRxiv - Developmental Biology 2020Quote: ... and 0.1 % Penn/Strep (Gibco 15140-122). Cells were maintained at passage numbers < 20 as recommended by the supplier and from previous handling experience.
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bioRxiv - Immunology 2022Quote: ... and 0.35% Pen Strep (Gibco 15140-122). Media was filtered using a 0.2-μm bottle filter and warmed to 37°C before being added to cells ...