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Citations for Thermo Fisher :
1301 - 1350 of 10000+ citations for Recombinant Human FCGRT & B2M Protein His Avi Strep II tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... 1% penicillin/streptomycin (Pen/Strep, Gibco), and 10% macrophage colony-stimulating factor (M-CSF ...
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bioRxiv - Biochemistry 2023Quote: ... and Pen Strep (Gibco, 15140-122). For passaging and experiments ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1% Pen-Strep (ThermoFisher #15140122). YUMM1.7D4 is abbreviated as YUMM1.7 and YUMMER1.7D4 as YUMMER ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1% pen-Strep (ThermoFisher# 15140122). Tumors were digested for 1 hr at 37°C in RPMI medium supplemented with 10% FBS ...
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bioRxiv - Biochemistry 2023Quote: ... and 1% Pen-Strep (Gibco 15140122). HeLa cells (ATCC CCL-2 ...
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bioRxiv - Bioengineering 2023Quote: ... and 1% Pen/Strep (ThermoFisher Scientific) 100 ng/ml human M-CSF (Peprotech ...
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bioRxiv - Neuroscience 2024Quote: ... 100 U/mL Pen/Strep (Gibco), 1 mM NaPyruvate (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... 100 U/mL Pen/Strep (Gibco), 5 µg/mL Insulin (Sigma) ...
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bioRxiv - Bioengineering 2024Quote: ... 1% Pen/Strep (Thermofisher 15140-122) (T cell media) ...
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bioRxiv - Neuroscience 2024Quote: ... and 5% Pen/Strep (Thermo Fisher) in a 5% CO2 incubator for 24-30 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1x Pen/Strep (Gibco 15140122).
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bioRxiv - Microbiology 2024Quote: ... 100U/ml pen/strep (Life Technologies), 2mM Glutamax (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... 1% Penicillin/Streptomycin (Pen/Strep; Gibco), 1% GlutaMAX (Gibco) ...
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bioRxiv - Immunology 2024Quote: Purified recombinant protein vaccines were analyzed on SDS-PAGE by staining with GelCode Blue Safe Protein Stain (Thermo Fisher Scientific). Proteins separated by SDS-PAGE were also transferred onto nitrocellulose membranes for immunoblotting ...
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bioRxiv - Cell Biology 2021Quote: ... full length N-terminally 3xFLAG tagged human KNSTRN ORF was cloned into pTREx-DEST30 vector by serial Gateway recombinations (Invitrogen) according to the manual ...
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bioRxiv - Cell Biology 2020Quote: Parent CHO-K1 and CHO-K1 cells stably expressing extracellular ACP-tagged human insulin receptors were maintained at 37°C with 5% CO2 in HAM’s F-12 medium (Thermo Fisher) supplemented 10% fetal bovine serum (FBS) ...
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bioRxiv - Neuroscience 2022Quote: DNA coding for full length HA-tagged human NEGR1 (NM_173808.2) was synthesized using the GeneArt® Gene Synthesis service (Life Technologies) and subcloned into the pcDNA3 vector ...
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bioRxiv - Biochemistry 2022Quote: Chimeric SHh precursor encoding HA-tagged NLuc fused to native and mutant forms of human Sonic HhC autoprocessing domain were assembled in pDisplay (Invitrogen). Fragments encoding Sonic HhC were inserted into pDisplay using PstI and XhoI ...
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Peptide Arrays of Three Collections of Human Sera from Patients Infected with Mosquito-Borne VirusesbioRxiv - Microbiology 2019Quote: ... The arrays were then incubated for 1 h with goat anti-human IgG tagged with Alexa Fluor® 488 (Invitrogen) as secondary antibody ...
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bioRxiv - Microbiology 2022Quote: ... The two pAcgp67-RBD (residues 333–530) plasmid with a C-terminal 8×His tag were transfected into Sf9 cells using Cellfectin II Reagent (Invitrogen) to produce the recombinant baculoviruses ...
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bioRxiv - Microbiology 2020Quote: ... using the primers in Table II and after digestion with BamHI and XhoI was cloned into pCDNA™3.1/myc-His A (Invitrogen). CHIKV structural genes were initially PCR amplified from pDONR21-CHKVstr (60 ...
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bioRxiv - Microbiology 2020Quote: ... using the primers described in Table II followed by restriction digestion with BamHI and XbaI and ligation into pcDNA-V5/His (Invitrogen). The MV H and HPIV-1 HN were PCR amplified using the primers shown in Table II from genomic RNA of MV ...
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bioRxiv - Developmental Biology 2021Quote: ... were coated with 10 μg/ml recombinant human laminin 521 (BioLamina) diluted in DPBS (Thermo Fisher Scientific) for 3 hours at 37°C and washed with DPBS twice ...
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bioRxiv - Neuroscience 2021Quote: ... Human recombinant full length wild type Flag-LRRK2 (#A15198) and Flag-LRRK2[G2019S] (#A15201) were from ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... and 5 μg/mL human recombinant laminin 521 (BioLamina #LN521-02) in E8 basal medium (Gibco #A1517001) supplemented with 1% Penicillin/Streptomycin (Pen/Strep ...
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bioRxiv - Cell Biology 2021Quote: ... anti-rabbit IgG antibodies (1:500 for IF) and recombinant human BMP2 (#PHC7145) were from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... and 5 μg/mL human recombinant laminin 521 (BioLamina #LN521-02) in E8 basal medium (Gibco #A1517001) supplemented with 1% Penicillin/Streptomycin (Pen/Strep ...
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bioRxiv - Immunology 2020Quote: ... M0 cells were treated for two days with 20 ng/ml recombinant human IFN-y (Life Technologies) and 50 ng/ml Escherichia coli LPS (SigmaAldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... The medium was supplemented with 15% fetal bovine serum (FBS) and recombinant human insulin (Gibco 12585-014) for pancreatic cancer cells and with 10% FBS for both human lung and colon cancer cells ...
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bioRxiv - Neuroscience 2024Quote: ... hiPS cells were cultured on six-well plates coated with recombinant human vitronectin (VTN–N, Gibco, A14700) in Essential 8 medium (Gibco ...
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bioRxiv - Molecular Biology 2020Quote: ... Biotinylated target proteins were purified with 25µL (slurry) of MyONE-C1 streptavidin beads (Thermo Fisher Scientific, 65002), pre-washed with 1x NLB + 1x PhosSTOP ...
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bioRxiv - Bioengineering 2020Quote: ... Detection of EDA positive fibronectin was performed using biotinylated fusion proteins followed by Streptavidin Alexa 488 (Invitrogen). In parallel the neovasculature marker CD31 was detected using either rat anti-mouse CD31 (BD Pharmingen ...
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bioRxiv - Neuroscience 2020Quote: Biotinylated cell-surface proteins were pulled down with streptavidin-coupled magnetic beads (#11205D; Thermo Fisher Scientific, US). The beads were isolated ...
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bioRxiv - Microbiology 2019Quote: ... Cells were lysed with RIPA buffer and biotinylated proteins were precipitated with streptavidin-agarose beads (Thermo Fisher). Elution was carried out with Laemmli buffer (2% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... the biotinylated proteins were purified by incubating the lysates with High Capacity Streptavidin Agarose beads (Pierce, ThermoFisher) overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Biotinylated proteins were detected with streptavidin coupled to Alexa Fluor 488 or 594 (1:1000; Life Technologies).
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bioRxiv - Immunology 2022Quote: ... purified HIVJR-CSF gp120 protein was biotinylated using EZ-Link Sulfo-NHS-LC-Biotin (Thermo Fisher Scientific) and excess biotin was removed using a Zeba spin desalting column (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... biotinylated protein signal was detected by incubation with streptavidin conjugated with Alexa Fluor 568 (Invitrogen, Cat# S11226) or 647 (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... and surface proteins were biotinylated with 0.5 mg/ml Premium Grade Sulfo-NHS-LC-Biotin (Fisher Scientific) in PBS at 4°C for 30 min ...
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bioRxiv - Biochemistry 2023Quote: ... N-terminally biotinylated 67B89 target protein was immobilized on Streptavidin Dynabeads M-270 (Invitrogen, Carlsbad, CA, USA). The target-coated beads were blocked with BSA and incubated with a suspension of 3.4·1012 phages in the presence of a 10-fold molar excess of the off-target (variant 6789) ...
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bioRxiv - Cell Biology 2023Quote: ... Cell surface proteins were biotinylated with 0.5mg/ml EZ-link-Sulfo-NHS-SS-Biotin (Thermo Fisher, A39258) in a PBS buffer with 2.5mM CaCl2 and 1mM MgCl2 on ice for 30 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... and the biotinylated cell surface proteins were affinity-purified on streptavidin magnetic beads (Thermo Scientific, cat#88816). After stripping off the nonspecifically bound proteins by several rounds of washing with the lysis buffer ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 (438-516) S-RBD((HiS)6 and biotynilated human ACE2 have been purchased from Fisher Scientific (respective references 16534204 and 16545164) ...
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bioRxiv - Biophysics 2019Quote: ... FLAG-tagged hZP3 and HA-tagged hZP4 were synthesized (GenScript; GeneArt/Thermo Fisher Scientific) and subcloned into pHLsec329 ...
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bioRxiv - Plant Biology 2022Quote: DNA templates of twin-strep-tag (TST) modified D1 and uS2c proteins were obtained from Invitrogen (GeneArt Gene Synthesis). Therefore ...
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bioRxiv - Cancer Biology 2019Quote: ... The fragments were digested in digestion medium (advanced DMEM/F12 with 2% FBS, Pen/Strep, 100 U/mL collagenase type XI [SigmaAldrich], and 125 μg/mL dispase type II [Invitrogen, Waltham, MA, USA]) at 37 °C for 40 min and then further digested for 10 min by adding a half-volume of TrypLE Express ...
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bioRxiv - Microbiology 2022Quote: ... were transfected with 500 ng of nsp14-strep plasmid encoding nsp14-strep using Lipofectamine 3000 (Thermo Fisher). The next day ...
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bioRxiv - Molecular Biology 2021Quote: ... for protein transfer (X-cell-II blot module, Thermo Fisher Scientific). After two hours ...
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bioRxiv - Molecular Biology 2023Quote: ... for protein transfer (X-cell-II blot module, Thermo Fisher Scientific). NC membrane was incubated in 5% skimmed milk ...
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bioRxiv - Immunology 2021Quote: ... and the ELISPOT plate was incubated for 4 hours at 4°C with biotinylated goat anti-human IgG Fc (Invitrogen), followed by incubation for 1 hour at room temperature with horseradish peroxidase (HRP)–conjugated avidin (Vector Laboratories) ...