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Citations for Thermo Fisher :
1401 - 1450 of 4028 citations for Influenza Virus Hemagglutinin HA H7N9 Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... The supernatant was collected and anti-HA magnetic beads (ThermoFisher Scientific) were added to the lysates followed by incubation for 30 min at room temperature on a tube rotator ...
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bioRxiv - Molecular Biology 2022Quote: ... HeLa-HA cells69 were grown in Minimum Essential Medium (MEM) (Gibco) supplemented with 10% FBS (Capricorn Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... 30 μL Pierce anti-HA Magnetic Beads (Thermo Fisher Scientific, #88836) were used for each sample ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-HA (clone 12CA5; 1:2000: Thermo Fisher MA1-12429), and rabbit anti-CDK1 pY15 (clone 10A11 ...
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bioRxiv - Cell Biology 2022Quote: ... 10mM EDTA) and 40ul anti-HA agarose bead slurry (Thermo Fisher) were added and incubated overnight at 4°C on a rotating shaker ...
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bioRxiv - Molecular Biology 2022Quote: ... equilibration of Pierce anti-HA agarose bead (Thermo Scientific, cat#26181) (~30μL packed volume per reaction ...
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bioRxiv - Microbiology 2022Quote: ... The HAs and NAs were synthesized by GeneArt (Thermo Fisher Scientific) and then subcloned into PHW2000 plasmids ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Mouse anti-HA magnetic beads (2 μl/well) (Thermo Fisher 88837) were washed in detergent buffer ...
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bioRxiv - Microbiology 2022Quote: ... HA was detected using a rabbit monoclonal antibody (71-5500, Invitrogen). Primary antibodies were detected with anti-mouse Alexa-Fluor 488 and anti-rabbit Alexa-Fluor 594 secondary antibodies (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... mouse anti-HA (1:500, monoclonal 2-2.2.14, Thermo Fisher Scientific), rabbit anti-SYP-2 (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... and blotted with mouse anti-HA (1:1,000, Thermo Fisher, #26183), Guinea pig anti-HTP-3 [1:1,500 ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were incubated overnight with anti-HA magnetic beads (Thermo Scientific) or for 5 h with GFP-Trap magnetic agarose (Chromotek ...
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bioRxiv - Microbiology 2023Quote: ... Protein was purified using purified anti-HA agarose (Thermo Fisher Scientific) as described above and fractions were analyzed by SDS-PAGE ...
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bioRxiv - Microbiology 2023Quote: ... Recodonized CDPK1 cDNA-HA sequence was synthesized (GeneArt strings, Life Technologies) and amplified with appropriate overhangs using primers P9/P10 ...
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bioRxiv - Immunology 2023Quote: HA proteins were transiently expressed in HEK 293F cells (Thermo Fisher) at a density of 1.0 x 106 cells/mL with a 1:3 ratio of DNA to PEIMax ...
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bioRxiv - Biochemistry 2023Quote: ... with a DyLight 550-conjugated anti-HA antibody (ThermoFisher, Waltham, MA). Cells were then fixed ...
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bioRxiv - Molecular Biology 2023Quote: ... and HA-488 (1:200; Life Technologies, Alexa Fluor SC-805). The next day ...
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bioRxiv - Microbiology 2023Quote: ... The FLAG-pTBK1-HA plasmid was generated by Gateway cloning (Invitrogen), using the FLAG-pTBK1 as template for generation of the entry clone (forward primer ...
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bioRxiv - Microbiology 2024Quote: ... FLAG-HA-SIVdeb Vpr was initially synthesised by GeneArt (Thermo Fisher) and cloned into our pLRGatewayIRESeYFP plasmid [46] using BamHI (into a BglII site ...
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bioRxiv - Cancer Biology 2021Quote: ... of Iowa) was prepared by diluting virus stocks to 400 p.f.u./µL into OptiMEM (Gibco) followed by addition of CaCl2 to a final concentration of 10 µM ...
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bioRxiv - Microbiology 2020Quote: ... and transfected for virus reconstitution into BHK-21 cells with Lipofectamine 3000 (ThermoFisher #L3000-075).
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bioRxiv - Microbiology 2020Quote: ... qPCR was performed using the TaqMan™ Fast Virus 1-Step Master Mix (Applied Biosystems). Assays were conducted on a QuantStudio5 Q-PCR machine (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2021Quote: ... The assay was performed using the TaqMan® Fast Virus 1-Step Kit (Life Technologies) and kit manual ...
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bioRxiv - Molecular Biology 2020Quote: ... a 20μL monoplex reaction contained 5μL of 4x TaqMan Fast Virus Master Mix (Thermo Fisher), 0.6µL of each primer (0.3μM final concentration each) ...
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bioRxiv - Immunology 2019Quote: ... two-fold virus stock dilutions were prepared in U-shaped 96-well plates (Thermo Scientific). The same volume of a 1% human O+ red blood cell suspension (Innovative Research ...
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bioRxiv - Microbiology 2019Quote: ... Other antibodies were obtained from commercial vendors as follows: Anti-coxsackie virus B3 antibodies (ThermoFisher), Anti-adenovirus A5 antibodies (ThermoFisher) ...
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bioRxiv - Immunology 2020Quote: ... RAW264.7 cells were then transduced with virus and selected using hygromycin at 10mg/ml (Invitrogen) to select for cells containing the shRNA plasmid.
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bioRxiv - Microbiology 2021Quote: ... and the number of virus particles was determined using a Nanodrop 2000 (Thermo Fisher Scientific). The adenovirus stocks were aliquoted and stored at -80°C.
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bioRxiv - Neuroscience 2021Quote: ... using TaqMan Fast Virus 1-Step Master Mix (PN4453800, Applied biosystems, Thermo Fisher Scientific, USA). PrimerTime primers and FAM-dye coupled detection probes were used for detecting Clstn3 mRNA level ...
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bioRxiv - Neuroscience 2021Quote: ... The final buffer for the virus was: dPBS + 5% Glycerol + 0.001 Pluronic F-68 (Invitrogen). The AAV titre (1.71 × 1013 GC/ml ...
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bioRxiv - Microbiology 2021Quote: ... Five μL RNA was tested with TaqManTM Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Neuroscience 2019Quote: ... The qPCR for Sendai virus detection was performed using TaqMan Gene Expression Assays (Life Technologies). Quantitative RT-PCR was performed using SYBR® Green PCR Master Mix (Applied Biosystems™ ...
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bioRxiv - Cell Biology 2022Quote: Cell lines were generated using Sendai virus CytoTune-iPS 2.0 Sendai reprogramming kit (Thermo Fisher) for transfection of Yamanaka’s factors ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μL of purified virus was analyzed with the Quant-it PicoGreen dsDNA Assay (Invitrogen).
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bioRxiv - Biochemistry 2022Quote: ... concentrated virus was run on a 4-20% Tris-Gly SDS-PAGE gel (Life Technologies), which was then analyzed using the Pierce Silver Stain Kit (Pierce ...
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bioRxiv - Immunology 2022Quote: ... For these assays we used TaqMan Fast Virus 1- Step Master Mix (ThermoFisher, catalog # 4444432) and custom primers/probes targeting the E gene sgRNA (forward primer ...
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bioRxiv - Molecular Biology 2022Quote: Viral RNA was extracted from amplified virus stock using Trizol reagent (Invitrogen; Cat no. #15596018). Reverse transcription was carried out using in-house produced Moloney murine leukemia virus (MMLV ...
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bioRxiv - Neuroscience 2021Quote: ... using TaqMan Fast Virus 1-Step Master Mix (PN4453800, Applied biosystems, Thermo Fisher Scientific, USA). PrimerTime primers and FAM-dye coupled detection probes were used for detecting Clstn3 mRNA level ...
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bioRxiv - Plant Biology 2021Quote: ... First-strand cDNA synthesis was performed using Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV, Invitrogen). PCR was performed in an AriaMx thermal cycler (Agilent ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... UKEi1-A was reprogrammed with a Sendai Virus strategy (CytoTune iPS Sendai Reprogramming Kit, ThermoFisher). These studies were approved by the Ethical Committee of the University Medical Center Hamburg-Eppendorf (Az ...
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bioRxiv - Immunology 2022Quote: ... RNA from virus-infected cells was isolated using PureLink RNA Mini Kit (Thermo Fisher Scientific), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... genomic DNA was extracted from 1010 virus using the PureLinkTM Genomic DNA mini kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... The virus was dialyzed with PBS supplemented with 0.05% of Pluronic F68 (Thermo Fisher Scientific) using 100-kDa filters (Millipore) ...
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: ... RNA was reverse transcribed (RT) using Moloney murine leukemia virus (MMLV) reverse transcriptase (#28025013, Ambion) and a mixture of anchored oligo-dT and random decamers (IDT) ...
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bioRxiv - Plant Biology 2019Quote: ... 10 IU of Moloney murine leukemia virus RNase H+ reverse transcriptase solution (Thermo Fisher Scientific), and appropriate buffer containing deoxynucleoside triphosphates (dNTPs ...
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bioRxiv - Cell Biology 2020Quote: ... Isolated total RNA was reverse transcribed with Moloney murine leukemia virus (MMLV) reverse transcriptase (Invitrogen) and oligo-dT primers ...
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bioRxiv - Microbiology 2021Quote: ... MRC-University of Glasgow Centre for Virus Research) were maintained in Leibovitz’s L15 media (Gibco) containing 10% FBS (Gibco) ...
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bioRxiv - Microbiology 2020Quote: Compound dilutions for virus treatment were performed in Minimum Essential Media (MEM) purchased by Gibco.
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bioRxiv - Microbiology 2019Quote: ... and transfected for virus reconstitution into BHK-21 cells with Lipofectamine 3000 (ThermoFisher #L3000-075). HSV-1 expressing the FLAG-tagged N-terminal extension of UL54 virus were reconstituted and titrated on Vero 2-2 cells50 ...
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bioRxiv - Microbiology 2019Quote: Concentrated virus aliquots were mixed with DiI (final concentration of 1 μM, Thermo Fisher, USA), vortexed and incubated for 1.5 h at room temperature (RT) ...