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Citations for Thermo Fisher :
1401 - 1450 of 10000+ citations for Estriol ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Wells were washed 3 times with TBS-T and phage binding was detected by addition of 20 µl SuperSignal™ ELISA Pico Chemiluminescent Substrate (Thermo-Fisher Scientific) and read on an Envision multilabel plate reader (Perkin-Elmer) ...
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bioRxiv - Microbiology 2023Quote: ... The final step was the addition of 100 µL of 1-Step Ultra TMB-ELISA Substrate solution (Thermo Scientific, catalogue number 34029) into wells for 5 minutes to allow a colorimetric reaction ...
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bioRxiv - Biochemistry 2023Quote: ... After centrifugation screening of soluble EDB-specific binders was performed by ELISA as follows: lysates were incubated with target-coated (67B89) and off-target-coated (6789) 96-well Medisorp-plates (Nunc, Roskilde, Denmark) followed by washing with PBST and PBS ...
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bioRxiv - Immunology 2023Quote: ELISA to detect specific anti-NP IgG1 antibodies was performed in 96-well flat bottom Nunc-ImmunoTM MaxiSorpTM plates (Thermo Fisher Scientific). Plates were incubated ON using 10 μg/mL of NP(2,5)-BSA or NP(25)-BSA as the coating reagent kindly provided by Dr ...
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bioRxiv - Bioengineering 2022Quote: ... Dam serum and placental tissue lysates (both prepared as described above) were assayed for IL-6 concentration using an ELISA per manufacturer instructions (Invitrogen, Waltham, MA). Sample absorbance values were compared to a standard curve to calculate IL-6 concentration.
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bioRxiv - Microbiology 2023Quote: ... Bacteria from day two plates were resuspended in PBS and adjusted to an OD 600 nm of 0.5 to coat high binding ELISA plates (Nunc-Immuno™, ThermoScientific). After incubation overnight at 4ºC ...
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bioRxiv - Microbiology 2023Quote: ... Formalin-killed N61 in PBS was dried in an oven at 5×106 cells/well in 96-well ELISA plates (C96 Maxisorpcert, Nunc-Immuno Plate, Thermo Fisher Scientific) overnight at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... ELISA signal was measured using a BioTek Synergy Neo microplate reader one minute after adding SuperSignal ELISA Pico Chemiluminescent substrate (ThermoFisher Scientific, 37069).
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bioRxiv - Neuroscience 2024Quote: ... Fifty microliters of unlabeled Lec-bs IgG diluted in TBS was used to coat an ELISA plate (Nunc, MediSorp, Thermo Fisher Scientific) overnight at 4°C with agitation ...
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bioRxiv - Neuroscience 2024Quote: ... Fifty microliters of unlabeled Lec-bs IgG diluted in TBS was used to coat an ELISA plate (Nunc, MediSorp, Thermo Fisher Scientific) overnight at 4°C with agitation ...
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bioRxiv - Molecular Biology 2021Quote: ... The diluted protein was coated in an increasing gradient (25-200 ng well-1) on a 96-microtiter ELISA plate (Nunc, Thermo Fisher Scientific) overnight at 4°C ...
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bioRxiv - Immunology 2019Quote: ... The reaction was stopped with 3 N H2SO4 and read at 492 nm on a MultiSkan GO ELISA plate reader (Thermo Fisher Scientific, Vantaa).
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bioRxiv - Molecular Biology 2019Quote: The protein concentrations of the ELISA were corrected for the total protein concentrations of the sample as determined by Qubit fluorescence incorporation protocol (Qubit, Life Technologies, CA, USA).
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bioRxiv - Cancer Biology 2019Quote: ... Positive phage clones were verified for preferential binding to plate-bound Fc-iNKG2D.YA or Fc-iNKG2D.AF versus Fc-wtNKG2D by spot ELISA and bound phage detected with biotinylated M13 phage coat protein monoclonal antibody E1 (Thermo Fisher # MA1-34468) followed by incubation with streptavidin-HRP.
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bioRxiv - Immunology 2021Quote: ... Cross-link activation of reporter cells was performed by direct coating of target antibody to ELISA plate (Nunc Maxisorp; 96 well, flat transparent), followed by a blocking step and incubation with 2 × 105 reporter cells per well ...
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bioRxiv - Immunology 2022Quote: ... IL-5 and IFN-γ were quantified using Invitrogen™ eBioscience™ ELISA Ready-SET-Go!™ (Thermo Fisher Scientific, Waltham, MA, USA). IP-10 (CXCL10) ...
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bioRxiv - Immunology 2020Quote: Culture supernatants at different time intervals post infection/ treatment were filtered through a 0.2μ filter and subjected to ELISA by using the eBioscience (Thermo Fisher Scientific Inc. USA) ELISA kit as per recommended protocols.
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bioRxiv - Cell Biology 2020Quote: The concentration of IFNλ1/IL-29 was measured in basolateral medium from RSV BT2a- or mock-infected cultures at 96 hpi by ELISA (Thermo Fisher Scientific; BMS2049). The manufacturer’s instructions were followed.
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bioRxiv - Biochemistry 2021Quote: ... The reaction was developed using TMB (3,3⍰,5,5⍰-Tetramethylbenzidine) substrate (1-Step™ Ultra TMB-ELISA Substrate Solution; Thermo Scientific, Waltham, MA, USA). Absorbance was measured at 450 nm with Multiskan™ FC Filterbased Microplate Photometer (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... Interleukin-8 (IL-8 or CXCL8) protein in the basolateral media was measured using a magnetic bead-based ELISA (Procartaplex, ThermoFisher Scientific, Waltham, MA) according to the instructions provided by the manufacturer and the plate were then read using a Luminex-based Bio-plex Multiplex system (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... diluted 1:3000 (PBS milk 1% -Tween 0.1%) for 1 h at 25°C and detected using 1-Step(tm) Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific, Waltham, MA). Plates were read using Cytation5 (BioTek ...
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bioRxiv - Immunology 2021Quote: ... plates were washed six times with PBST and developed by adding 50 μL of 1-StepTM Turbo-TMB-ELISA Substrate Solution (Thermo Fisher Scientific 34022) per well ...
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bioRxiv - Immunology 2020Quote: ... Native ECP was coated at 10 μg/ml (IgA) or 20μg/ml (IgG) on ELISA plates (Nunc Maxisorp™, ThermoFisher Scientific, Waltham, MA). Bile was diluted to 1:200 and serum was diluted to 1:20 in PBS containing 2.5 % ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed 3 times with WB before adding 100 µL 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Fisher 34029) followed by incubation in the dark at room temperature with shaking (225 rpm ...
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bioRxiv - Neuroscience 2023Quote: ... SAP97 or SAP102 fused to a C-terminal MBP were coated overnight at 4°C on ELISA plates (Nunc Maxisorb Immunoplates, #442404, Thermo) at a concentration of 100 ng per well ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then incubated for 24 hours to induce macrophage activation and cytokine release.48 ELISA (Invitrogen, Waltham, MA, Product #88-7066-22) was performed to quantify the level of IL-6 present in the cell media ...
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bioRxiv - Biochemistry 2023Quote: ... three times washed with 1%BSA (prepared in 1XTBS) followed by development of signal by treating cells with 200μL TMB-ELISA (Thermo Scientific, Cat no. 34028) until the light blue color appeared ...
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bioRxiv - Bioengineering 2022Quote: ... Plates were finally washed three more times with PBS-T and developed using 1-Step Ultra TMB-ELISA solution (Thermo Fisher Scientific #34028). The HRP reaction was stopped with 1M hydrochloric acid and absorbance values at 450 nm were measured on a SpectraMax i3x plate reader (Molecular Devices).
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bioRxiv - Immunology 2023Quote: ... E40500) to stop the reaction and the absorbance was measured at 450/620 nm using an ELISA plate reader (Thermo Fisher, Varioskan LUX). The mean OD (optical density ...
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bioRxiv - Immunology 2020Quote: Cytokine concentrations for IL-17A and IFNγ from the supernatants of cultured cells and ex vivo colon organ cultures were measured by ELISA (Thermo Fisher Ready-Set-Go). Samples and standards were measured in duplicate ...
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bioRxiv - Biochemistry 2021Quote: ... plates were washed 4 times with TBS-T and proteins were detected by addition of SuperSignal™ ELISA Femto substrate (ThermoFisher cat. no. PI37074). Luminescence was read using a BMG POLARstar Omega plate reader or Biotek Neo2 microplate reader.
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bioRxiv - Microbiology 2020Quote: Chimeric proteins were diluted to 1 μg/ml in carbonate buffer (pH 9.6) and used to coat polystyrene 96-well high-binding ELISA plates (100 μl/well; Nunc-Immuno plate with MaxiSorp surface). After overnight incubation at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... Mouse cytokine levels in vaccinated animals (IL-4, IL-10, IL-12, and IFN-γ) was measured by ELISA following the supplier’s protocol (Invitrogen or R&D Systems).
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bioRxiv - Microbiology 2020Quote: Rabbit serum immunized with DENV-1 Mochizuki strain (in-house product) was coated onto a Nunc Maxisorp ELISA plate (Thermo Fisher Scientific, Waltham, MA). Then ...
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bioRxiv - Pathology 2022Quote: ... The reaction was stopped with 3N H2SO4 (50 μL/well) and read at 492 nm on a MultiSkan GO ELISA plate reader (Thermo Fisher Scientific, Vantaa, Finland) [35].
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bioRxiv - Bioengineering 2019Quote: ... conditioned media collected from cells was assessed with enzyme-linked immunosorbent assays (ELISA) in order to quantify the concentration of extracellular tumor necrosis factor alpha (TNFα, Invitrogen, cat. #88-7346-88) or Dex (Bioo Scientific ...
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bioRxiv - Immunology 2020Quote: ... were diluted in 50 mM carbonate buffer to a concentration of 1 μg/ml and coated at 50 ng/well on 96-well ELISA plates (MaxiSorp Nunc, Thermo Fisher Scientific K.K., Japan) overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... incubated at RT for 10 min and the absorbance was recorded at 540 nm using a 96-well plate ELISA reader (Thermo Fisher, Rockford, IL, USA).57 The concentration of nitrite was calculated according to the standard curve generated from known concentrations of sodium nitrite dissolved in fresh culture media.
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bioRxiv - Microbiology 2021Quote: ... Specifically, 1 μg/ml purified hACE2 protein (Kactus, ACE-HM501) was coated onto a 96-well ELISA plate (Thermo Fisher Scientific, 44-2404-21) at 4°C overnight ...
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bioRxiv - Microbiology 2021Quote: ... The bacteria were cultured at 37°C for 48 hours and the concentrations were measured at multiple time points by the ELISA plate reader (Multiskan Go, Thermo Scientific, Waltham, MA, USA) at the OD of 600nm.
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bioRxiv - Immunology 2023Quote: Peptide competitions were undertaken by incubating RAVV GP ΔMuc at 200 ng per a well in Nunc MaxiSorp 96 well ELISA plates (Thermo Fisher Scientific, Waltham, MA) overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Direct pepscan analysis was undertaken by binding 200 ng of each overlapping peptide to Nunc MaxiSorp 96 well ELISA plates (Thermo Fisher Scientific, Waltham, MA) overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... and the bound Arr3_ýC•IP6–Fab7 complexes incubated with HRP-conjugated Pierce recombinant protein L (cat: 32420, Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
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bioRxiv - Biochemistry 2024Quote: ... and the bound NTCP-Fab complexes were incubated with a secondary HRP-conjugated Pierce recombinant protein L (Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
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bioRxiv - Immunology 2019Quote: ... Supernatants were collected following a 48h incubation at 37°C and IFNγ and TNFα levels were measured by ELISA (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Neuroscience 2020Quote: ... Blots were developed using Amersham ECL Prime Western Blotting Detection Reagent (RPN2232) or SuperSignal ELISA Femto Maximum Sensitivity Substrate (Thermo Scientific, Ref: 37075, Lot: UO282555) and the resulting chemiluminescence imaged with an Alpha Innotech MultiImage Light Cabinet (Alpha Innotech ...
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bioRxiv - Immunology 2020Quote: ... were diluted in 50 mM carbonate buffer to a concentration of 1 μg/ml and coated at 50 ng/well on 96-well ELISA plates (MaxiSorp Nunc, Thermo Fisher Scientific K.K., Japan) overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... and the bound target-Fab complexes were incubated with an HRP-conjugated Pierce recombinant protein L (cat: 32420, Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... Formalin-killed N61 in PBS was dried in an oven at 5×106 cells/well in 96-well ELISA plates (C96 Maxisorpcert, Nunc-Immuno Plate, Thermo Fisher Scientific) overnight at 37°C ...
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bioRxiv - Plant Biology 2024Quote: Coating: Prepared sample were added to the selected ELISA-suitable antigen-binding 96-well microtiter plate (CORNING COSTAR 9018; Thermofisher scientific Ref: 44-2504-21). The first two columns contained the peptide standard ...