Labshake search
Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for Estriol ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... The washing procedure was repeated and 50 μL of the buffer containing substrate (1-Step Ultra TMB ELISA Substrate, Thermo Scientific) was added to develop the colorimetric reaction for 15 min ...
-
bioRxiv - Bioengineering 2019Quote: ... The antibodies were diluted to 1 µg/ml in PBS and 100 µl of this solution per well were applied to the Nunc MaxiSorp ELISA plate (Thermo Fisher) for coating overnight ...
-
bioRxiv - Immunology 2019Quote: ... 50 μL of HLA binding neutralisation buffer was added to each well and the resulting supernatants were incubated for 2 hours at room temperature in ELISA plates (Nunc, Denmark) previously coated with 10 μg/mL of the monoclonal antibody L243 ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... 1/512) and 50μl of each dilution was added to Immulon™ 2 HB flat bottom 96-well ELISA plates (Thermo Scientific) in triplicate ...
-
bioRxiv - Microbiology 2021Quote: ... The peroxidase reaction was terminated by adding 50 μL of H2SO4 (1 M) before the final optical density (OD) was taken using an ELISA plate reader (Multiskan Go, Thermo Scientific) at the wavelength of 450 nm ...
-
bioRxiv - Cell Biology 2022Quote: ... The plate was then washed several times with 250 μl of PBS-0.05% Tween-20 and incubated with 100 μl of SuperSignal ELISA Pico chemiluminescent substrate (Thermo Scientific #37069). The resulting signal was read 10 minutes post addition of the substrate For quantification ...
-
bioRxiv - Molecular Biology 2020Quote: ... The washing procedure was repeated and 50 μL of the buffer containing substrate (1-Step Ultra TMB ELISA Substrate, Thermo Scientific) was added to develop the colorimetric reaction for 15 min ...
-
bioRxiv - Microbiology 2021Quote: CVL supernatants were recovered by centrifugation for assessment of IL-8 levels by Enzyme-Linked Immunosorbent Assay (ELISA) (Invitrogen, Paisley, UK), following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... Determination of SARS CoV-2 spike protein-specific human IgG antibody titers was performed in Maxisorp Immuno 96-well ELISA plates (Thermo Scientific) coated overnight at 4°C with 100 μl/well of 2 μg ml−1 recombinant SARS CoV-2 spike protein produced and purified from 293 cells in PBS ...
-
bioRxiv - Bioengineering 2020Quote: Human adiponectin was measured in the plasma of NSG mice was measured using a human-specific adiponectin ELISA from Invitrogen (KHP0041).
-
bioRxiv - Microbiology 2019Quote: ... A final washing step after 1 h incubation at TR was followed by addition of 100 µl 1-Step Slow TMB-ELISA substrate (Thermo Scientific) to each well and absorbance was measured after 10 min incubation at TR using the Bio-Rad Model 680 Microplate Reader (655 nm ...
-
bioRxiv - Synthetic Biology 2020Quote: ... were used for overnight coating of individual wells in a MaxiSorp high protein-binding capacity 96-well ELISA plate (Nunc, Denmark) at 4 °C ...
-
bioRxiv - Biochemistry 2021Quote: ... Serum Fab concentration was measured 7 days post HDD by ELISA using a goat anti-kappa light chain antibody (ThermoFisher Scientific).
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... for 48 h and cytoplasmic DNA was extracted according to the protocol as previously described.35 The cytoplasmic DNA lesion and plasma levels of DNA damage were measured using the DNA Damage Competitive ELISA (Thermo Scientific) according to the manufacturer’s instruction ...
-
bioRxiv - Bioengineering 2021Quote: ... plates were washed thrice with PBS-T and 20 μL of freshly prepared SuperSignal ELISA Pico Chemiluminescent Substrate (Thermo Scientific, #37069) (mixed 1:1 ratio and diluted in equal volume with dH2O ...
-
bioRxiv - Biochemistry 2020Quote: ... ELISA wells were washed four times with PBS-T and incubated with 50 μL/well of anti-GST (Invitrogen MA4-004) or anti-MAR (Millipore-Sigma MAB1076 ...
-
bioRxiv - Immunology 2021Quote: ... Cell culture supernatants were collected for ELISA assays and RNA was extracted from infected cells using Trizol (ThermoFisher; catalog no. 15596026) following manufacturer’s instructions.
-
bioRxiv - Immunology 2020Quote: ... Plates were washed 5 times with washing buffer and developed by addition of 100µL 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Scientific) for 3 min ...
-
bioRxiv - Immunology 2020Quote: ... TNFα and IL-23 expression in the culture medium was examined using an ELISA (Invitrogen for TNFα Biolegend for IL-23).
-
bioRxiv - Immunology 2020Quote: ... The MMP Luminex panel (PPX-05/ PROCARTAPLEX MMP1, MMP2, MMP3, MMP9 and MMP13 plex) and IL-6 Elisa (both from Life Technologies) was performed on co-cultures supernatants.
-
bioRxiv - Microbiology 2021Quote: ... diluted 1:3000 in 1% PBS-milk supplemented with 0.1% Tween-20 for 1 h at 37C and detected using 1-Step Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific). Plates were read using a Cytation 5 imager (BioTek ...
-
bioRxiv - Microbiology 2020Quote: Rabbit serum immunized with the DENV-2 NGC strain was coated onto Nunc Maxisorp ELISA plates (Thermo Fisher Scientific, Waltham, MA). Then ...
-
bioRxiv - Microbiology 2022Quote: ... plates were washed 3 times and then developed by adding 100 µl/well of TMB-ELISA substrate (ThermoFisher Scientific, catalog# 34029). Reactions were stopped after 10 minutes by adding 50 µl/well of 3M H2SO4 to all the wells ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: Venom was diluted in 100 mM carbonate-bicarbonate coating buffer pH 9.6 and coated at 100 ng per well in 96-well ELISA plates (Nunc MaxiSorp, ThermoScientific) and incubated overnight at 4°C ...
-
bioRxiv - Immunology 2024Quote: ... plates were washed 3 times with PBST and added with 50 μL of 1-Step Ultra TMB-ELISA (ThermoFisher, Cat. #34028). Reactions were stopped by the addition of 50 μL of 2 M sulfuric acid ...
-
bioRxiv - Microbiology 2024Quote: ... SARS-CoV-2 RBD antigens expressed in 293T cells were used to coat the 96-well ELISA plates (Cat No. 44240421, Thermofisher, USA). 50µL (2µg/mL ...
-
bioRxiv - Microbiology 2024Quote: ... plates were washed 3 times with PBST and 50 μL of 1-Step Ultra TMB-ELISA was added (ThermoFisher Cat. #34028). Following a 2 to 5-min incubation ...
-
bioRxiv - Neuroscience 2024Quote: ... Triton- and Guanidine-soluble samples were subjected to analysis using species- and isoform-specific antibodies in Soluble and Insoluble Aβ ELISA (Invitrogen, Life Technologies; Grand Island ...
-
bioRxiv - Biochemistry 2024Quote: ... RNA oligos were diluted to 100 nM in ELISA blocking buffer (PBS, 0.05% Tween-20, 0.2 mg/ml bovine serum albumin (BSA, BP9706100; Fisher Scientific; Hampton, NH)) ...
-
bioRxiv - Bioengineering 2024Quote: ... and RSV B/ 18537 DS-Cav1 (51) at 2 µg/mL were coated onto 96 well ELISA plates (Immulon 2 HB, Thermo Fisher) at 4°C overnight ...
-
bioRxiv - Molecular Biology 2023Quote: ... content in isolated mitochondria from WT and APP cells was measured by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer instructions (ThermoFisher khb3544).
-
bioRxiv - Microbiology 2022Quote: IgG binding to SARS-CoV-2 or MeV antigens was measured by ELISA using clear flat-bottom immuno nonsterile 96-well plates (Cat# 442404, ThermoFisher Scientific) coated overnight at 4°C with 100 ng of recombinant SARS-CoV-2 proteins or 1 μg of MeV bulk antigen (Cat# BA102VS ...
-
bioRxiv - Microbiology 2023Quote: ... plates were washed 3 times with PBST and 50 μL of 1-Step Ultra TMB-ELISA was added (ThermoFisher Cat. #34028). Following a 2 to 5-min incubation ...
-
bioRxiv - Cancer Biology 2023Quote: ... Absorbance was taken at 590 nm with a reference filter of 620 nm using an ELISA reader (Multiskan Spectrum Microplate Reader, Thermo Scientific). All data were normalized against DMSO blank controls.
-
bioRxiv - Cancer Biology 2023Quote: ... Using the standard curve from the ELISA and the amount of gDNA/sample (quantified by Nanodrop Spectrophotometer from Thermo Fisher Scientific), percentage of methylated DNA (5-mC ...
-
bioRxiv - Molecular Biology 2023Quote: ... except for antibodies used for detection in ELISA and flow cytometry. Genetic constructs were synthesized by Eurofins Genomics. Plasmid DNA midiprep (cat. K210004) was purchased from Thermo Fisher Scientific ...
-
bioRxiv - Bioengineering 2024Quote: ... to peptides detected using DECODE was performed as follows: 12.5µL per well of 4 mM peptide solutions were coated on 384 well ELISA plates™ (Thermo Fisher Scientific) by shaking at r.t ...
-
bioRxiv - Biochemistry 2024Quote: IL-6 secretion from THP-1-derived macrophages was quantified in the growth media using enzyme-linked immunosorbent assay (ELISA; Invitrogen, #BMS213INST) following the manufacturer’s protocol.
-
bioRxiv - Cell Biology 2024Quote: ... was added per well and incubated for 2-5 minutes before adding 100 µL the ELISA stop solution (Thermo Fisher Scientific). Absorbance was measured on the Varioskan microplate reader at 450 nm (Thermo Fisher Scientific).
-
bioRxiv - Molecular Biology 2021Quote: ... The diluted protein was coated in an increasing gradient (25-200 ng well-1) on a 96-microtiter ELISA plate (Nunc, Thermo Fisher Scientific) overnight at 4°C ...
-
bioRxiv - Microbiology 2021Quote: ... Plates were then washed twice with ELISA wash buffer to remove any excess unbound spike protein and blocked with 2% BSA (ThermoFisher Scientific; 37525) in ELISA wash buffer overnight at 4°C ...
-
bioRxiv - Neuroscience 2020Quote: Levels of Aβ40 in supernatants of HEK or HEKswe cells transfected with different MT5-MMP variants and/or C99 constructs or treated with C3 or DAPT were evaluated by ELISA (#KHB3481, Thermo Fisher Scientific) according to the manufacturer’s recommendations.
-
bioRxiv - Molecular Biology 2020Quote: ... Commercially available ELISAs for quantitative detection of human total IgG were used according to manufacturer’s protocols (Thermo Fisher Scientific, Waltham, MA, U.S.A.). All plates were read in a microtiter plate reader at 450 nm and optical density values were used in statistical analyses.
-
bioRxiv - Bioengineering 2020Quote: ... The blood samples were then centrifuged at 2000 xg for 10 minutes and serum collected for TNF determination by ELISA (Invitrogen, Carlsbad, CA) following the manufacturer’s instructions.
-
bioRxiv - Microbiology 2022Quote: ... Recombinant trimeric ectodomain of S protein with six proline mutations (rtS-ecto6P) for enzyme-linked immunosorbent assay (ELISA) was produced using Expi293F cells (Thermo Fisher Scientific). In addition to the mutation at the furin cleavage site ...
-
bioRxiv - Immunology 2020Quote: ... according to the manufacturer’s protocol with some adjustments: Thermo Scientific Nunc MaxiSorp 96 well ELISA plates were coated with 50 μg/mL Calf thymus DNA (Thermo Fisher Scientific), Phosphorylcholine (Sigma Aldrich) ...
-
bioRxiv - Immunology 2022Quote: ... Purified molecules or mouse anti-human IL-15 antibodies were coated on a MaxiSorp™ 96-well flat-bottom ELISA plate (ThermoFisher Scientific) for 12h and for 72h ...
-
bioRxiv - Immunology 2022Quote: ... then assayed in technical duplicate for secreted chemokines and cytokines by ELISA (IL-15, BAFF, CXCL10/IP-10, and CXCL11/I-TAC, all R&D Systems; CXCL9/MIG, Invitrogen) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2024Quote: ... Wells were washed 5 times with 200 µL PBS and developed by adding 100 µL of 1-Step Turbo TMB ELISA (Thermo Fisher Scientific) for 30 minutes at RT followed by stopping the reaction with 50 µL of 2M H2SO4 and reading optical density at 450 nm using BioTek ELx800 plate reader.
-
bioRxiv - Molecular Biology 2023Quote: ... Wells were washed 3 times with TBS-T and phage binding was detected by addition of 20 µl SuperSignal™ ELISA Pico Chemiluminescent Substrate (Thermo-Fisher Scientific) and read on an Envision multilabel plate reader (Perkin-Elmer) ...