Labshake search
Citations for Thermo Fisher :
1401 - 1450 of 10000+ citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... followed by two 5-min washing steps with washing buffer B (2X SSC and 10% formamide, Ambion; AM9342). In a humidified chamber ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were kept overnight at 4°C in IMDM medium supplemented with 10% FBS (HyClone) and 1% penicillin-streptomycin (Thermofisher). Staining was performed for 20min at room temperature (RT ...
-
bioRxiv - Genomics 2022Quote: ... 80% confluent flasks of HEK293FT cells were split 1:4 into DMEM culture medium [10% Fetal Bovine Serum (cat. #10437-028, Gibco), 1% PenStrep (cat ...
-
bioRxiv - Cell Biology 2019Quote: Cells were fixed in PKM buffer (10 mM Sodium Phosphate, 140 mM KCl and 1 mM MgCl2, pH 7.2) containing 4% paraformaldehyde (Thermo Fisher) for 10 min at room temperature followed by permeabilization in 0.2% Triton X-100 for 5 – 10 min ...
-
bioRxiv - Cell Biology 2019Quote: ... Cells were fixed in PKM buffer (10 mM Sodium Phosphate, 140 mM KCl and 1 mM MgCl2 pH 7.2) containing 4% paraformaldehyde (Thermo Fisher) for 10 min at room temperature followed by permeabilization in 0.2% Triton X-100 for 5 – 10 min ...
-
bioRxiv - Genetics 2019Quote: ... 1.25×10-4 U/µl DNase I and 1 U/µl DNA polymerase I (both ThermoFisher Scientific, Waltham, MA, USA). The reaction was incubated at 15 °C for 40 min and then purified using an illustra Sephadex G-50 column (GE Healthcare ...
-
bioRxiv - Cancer Biology 2020Quote: ... All cell lines were maintained in DMEM (25 mM Glucose, 4 mM Glutamine, 1 mM Sodium Pyruvate) supplemented with 10% Fetal Bovine Serum (FBS, Gibco) and 50 units/mL Penicillin/Streptomycin (Gibco ...
-
The MuSK-BMP pathway maintains myofiber size in slow muscle through regulation of Akt-mTOR signalingbioRxiv - Cell Biology 2022Quote: ... Serum was diluted 1:1000 in 1/10 block overnight at 4°C and detected using goat anti-human IgG Alexa Fluor 555 (Invitrogen) diluted 1:1000 and counterstained with AlexaFluor488-conjugated α-bungarotoxin (Thermo Fisher ...
-
bioRxiv - Biophysics 2023Quote: ... incubated in 10 mg/mL sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (sulfo-SMCC, Thermo Fisher Scientific, Waltham, MA, USA) at room temperature for 30 min ...
-
bioRxiv - Physiology 2024Quote: ... sections were incubated overnight at 4°C in primary antibody solution containing 10% goat serum and antibodies against laminin β1 (1:100; MA5-14657, Invitrogen) or laminin β1γ1 (1:200 ...
-
bioRxiv - Molecular Biology 2024Quote: ASOs were removed from 4 µl of DMS-modified RNA in 10 µl containing 1 µl of TURBO DNase (Invitrogen) and 1X TURBO DNase Buffer (Invitrogen) ...
-
bioRxiv - Neuroscience 2021Quote: ... or in 9:1 ethanol:formaldehyde (Fisher Scientific) for 2 hours at room temperature in a shaker ...
-
bioRxiv - Cell Biology 2023Quote: ... Myosin 9 HC (Thermofisher 5D9D2, 1:200).
-
bioRxiv - Biochemistry 2021Quote: ... Using a RP monolithic column (ProSwift™ RP-4H, 1×250 mm, Thermo Fisher Scientific, Bremen, Germany), solvent B started with 5% for a duration of 2 min ...
-
bioRxiv - Cell Biology 2019Quote: ... 1 × B-27 (Gibco), 1.25 mM N-acetylcysteine (Sigma) ...
-
bioRxiv - Cancer Biology 2022Quote: ... 1× B-27 (GIBCO), 1× N2 (GIBCO) ...
-
bioRxiv - Microbiology 2019Quote: ... 1 % amphotericin B (Gibco), and 20 ng/mL macrophage colony-stimulating factor (mCSF ...
-
bioRxiv - Microbiology 2020Quote: ... 1% amphotericin B (Gibco)] ...
-
bioRxiv - Cancer Biology 2023Quote: ... 1% B-27 (Gibco), 1% GlutaMAX (Gibco) ...
-
bioRxiv - Microbiology 2023Quote: ... 1× B-27 (Gibco), 1× N-2 (Gibco) ...
-
bioRxiv - Cell Biology 2019Quote: The staining of limiting membrane of the yeast vacuole was achieved with FM™ 4-64 Dye (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) (Invitrogen). Cells were grown to early-log phase in appropriate medium ...
-
bioRxiv - Microbiology 2023Quote: ... Culture (10 mL) was pelleted (10 min, 4000 xg, 4°C) and resuspended in 1.0 mL Trizol (Invitrogen). Cells were lysed by beat-beating in a MagnaLyser (30 s ...
-
bioRxiv - Genomics 2023Quote: ... coated dishes in N2 medium (high-glucose DMEM, 1% N-2, 2% B-27 and 1% penicillin/streptomycin, from Thermo Fisher Scientific) at a density of 2×105 cells/cm2 ...
-
bioRxiv - Cell Biology 2020Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxy-d-glucose (2-NBDG) was purchased from Invitrogen (Carlsbad, CA, USA). Trypsin-EDTA solution was purchased from GIBCO BRL (Grand Island ...
-
bioRxiv - Cancer Biology 2023Quote: To detect DNA synthesis EdU (5-ethynyl-2′-deoxyuridine; 10 μM, Invitrogen) was added to the medium and incubated for 24 h from days 8 to 9 post-treatment ...
-
bioRxiv - Molecular Biology 2019Quote: ... To initiate transcription 4 µL of 5 X first strand buffer (Invitrogen, Cat No. 1889832) and 1 µL each of 0.1 M DTT (Invitrogen ...
-
Potassium channel-driven bioelectric signaling regulates metastasis in triple-negative breast cancerbioRxiv - Cancer Biology 2021Quote: ... CDH11 #4: 5’-CCUUAUGACUCCAUUCAAA-3’ using Lipofectamine RNAiMAX transfection reagent (13778030; ThermoFisher Scientific, Waltham, MA) in serum-free DMEM ...
-
bioRxiv - Biochemistry 2021Quote: ... then fixed with 4% paraformaldehyde containing 5 µg/ml Hoechst 33342 stain (Thermo Fisher, #62249) for 10 min at room temperature ...
-
bioRxiv - Biochemistry 2021Quote: ... 5-7 μg BACMID DNA was incubated with 4 μL Fugene (Thermo Fisher, Cat# 10362100) in 200 μL of ESF 921 media for 30 minutes at 23°C ...
-
bioRxiv - Neuroscience 2021Quote: ... and incubated overnight (4° C) with 5 µg of Cx36 (Thermofisher, 37-4600, RRID: AB_2533320) or NMDAR1 (Thermofisher ...
-
bioRxiv - Microbiology 2021Quote: ... and 4 mg/l 5-(and-6)-carboxyfluorescein and succinimidyl ester (FITC; Thermo Fisher Scientific), respectively ...
-
bioRxiv - Neuroscience 2022Quote: ... for 5 min and loaded onto 4%–12% Nupage Bis-Tris gels (Novex/Life Technologies). Proteins were transferred using the iBlot2 (Life Technologies ...
-
bioRxiv - Microbiology 2022Quote: ... 4-5 ×106 P25 TCR-Tg CD4+ T cells (CD45.1) were labeled with CellTrace (Invitrogen) and adoptively transferred by tail vein injection in 100 µL of sterile PBS 24 hours (h ...
-
bioRxiv - Cancer Biology 2023Quote: ... for 5 min Samples were run down a Novex 4–12% Tris-glycine gel (Invitrogen) and transferred onto a nitrocellulose membrane (Bio-Rad ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were passaged every 4-5 days by treatment with StemPro Accutase (ThermoFisher, Cat#A1110501), stored in liquid nitrogen ...
-
bioRxiv - Microbiology 2023Quote: ... the 11.5 μL RNA were supplemented with 4 μL 5× first strand buffer (ThermoFisher Scientific), 1 μL 10 μM reverse primer ...
-
bioRxiv - Neuroscience 2024Quote: ... and cells were split every 4–5 days using 0.5 mM EDTA (Invitrogen, 15575-020). hiPS cell clones/lines were also generated using retroviruses or non-integrating episomal vectors from fibroblasts (Bhaduri et al. ...
-
bioRxiv - Bioengineering 2024Quote: ... adding 5 mM GMP and 4 U/µL RNaseOUTTM recombinant ribonuclease inhibitor (Thermo Fisher Scientific) to the transcription reaction ...
-
bioRxiv - Cell Biology 2022Quote: ... at 37°C in a 5% CO2 incubator with daily media changes and were passaged every 4-5 days using TrypLETM (ThermoFisher), following manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2021Quote: ... or 10 μM 7-hydroxy-9H-(1,3-dichloro-9,9-dimethylacridin-2-one) succinimidyl ester (DDAO-SE) (Invitrogen) in HMI11 and incubated for 20 min at 37°C ...
-
bioRxiv - Cell Biology 2021Quote: ... and one-fifth of the affinity purification (IP) were separated on Bolt 4-12% Bis-Tris Plus 15-well gels (Invitrogen), run in Bolt 1x MOPS SDS running buffer (Novex ...
-
bioRxiv - Microbiology 2021Quote: ... Genomic DNA was eliminated from RNA extracts by digesting for one hour at 37°C with 4 U of TURBO DNAase (Ambion) followed by purification with MEGAclear Transcription Clean-Up kits (Ambion).
-
bioRxiv - Microbiology 2021Quote: ... Protein purity was determined by resolving one to two micrograms of purified protein by SDS-PAGE using NuPAGE 4–12 % Bis Tris precast gels (ThermoFisher) for 50 minutes at 200 V ...
-
bioRxiv - Genomics 2022Quote: ... We estimated DNA quality using a NanoDrop One Microvolume UV-Vis Spectrophotometer and quantified DNA using a Qubit 4 Fluorometer (ThermoFisher) with a Quant-iT dsDNA HS Assay Kit ...
-
bioRxiv - Microbiology 2022Quote: ... Protein purity was determined by resolving one to two micrograms of purified protein by SDS-PAGE using NuPAGE 4–12% Bis Tris precast gels (ThermoFisher) for 50 minutes at 200V ...
-
bioRxiv - Cell Biology 2019Quote: ... Mixed protein eluates were separated by one-dimensional gel electrophoresis on a 4–12% NuPage Novex Bis–Tris gel (Invitrogen), stained with the Colloidal Blue Staining Kit (Invitrogen ...
-
bioRxiv - Immunology 2023Quote: ... One to 20 μg of total protein were separated by NuPAGE™ 4-12% Bis-Tris Gel (Thermo Fisher Scientific) and transferred onto PVDF membranes (Bio-Rad ...
-
bioRxiv - Biochemistry 2023Quote: ... 16 mL of HMA-10 buffer was added and the preparation was put on a shaking rocker for one hour at 4 °C with 3 units/mL Rnase-free Dnase (Ambion) added ...
-
bioRxiv - Bioengineering 2022Quote: ... cells were rinsed in DPBS and incubated with 2 μg/ml DAPI (4′,6-diamidino-2-phenylindole, 62248; ThermoFisher) and 1:500 Alexa Fluor Plus 647 conjugated goat anti-rabbit secondary antibodies (A32733 ...
-
Mycobacteria form viable cell wall-deficient cells that are undetectable by conventional diagnosticsbioRxiv - Microbiology 2022Quote: ... Nucleic acids were stained using 2 µM SYTO 9 (S34854, Invitrogen). The plasma membrane was labelled using SynapseRed C2M (SynapseRed ...