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Citations for Thermo Fisher :
1401 - 1450 of 10000+ citations for 6 THIOPHEN 2 YL 1H INDAZOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Enzyme assays were conducted in 0.5 mL containing 50 mM 2-(N-morpholino)ethanesulfonic acid (pH 6) and various concentrations of soluble starch (Acros Organics, No. AC424495000, Morris Plains, NJ). The assays also included 100 mM KCl ...
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFUSE-vector (Invitrogen, Thermo Fischer Scientific, MA, USA). The human ACE2 was expressed by essentially the same protocol used for the COVID-19 RBD ...
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bioRxiv - Genomics 2021Quote: ... The embryoid medium was aspirated and 15 to 20 embryoid bodies were plated per well in 6-well plates and cultured in 3 mL hematopoetic medium (2 mM GlutaMax, 1x Antibiotic-Antimycotic (Thermo Fisher Scientific; Cat. No. 15240062), 55 mM 2-mercaptoethanol (BioRad ...
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bioRxiv - Cancer Biology 2022Quote: ... To quantitate cell migration 6 fields of view per membrane were photographed with EVOS™ FL Auto 2 Imaging System(Thermo Scientific™ Invitrogen™), followed by quantification by ImageJ v1.53q (National Institutes of Health ...
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bioRxiv - Neuroscience 2024Quote: ... then separated on a reverse-phase nano-spray column (2 μm, 75 mm i.d. × 50 cm, 100 Å, Thermo Fisher Scientific Cat# 16-494-6) using gradient elution ...
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bioRxiv - Microbiology 2020Quote: ... Four to six-day old mated female mosquitoes were allowed to feed for 1h on approximately 108 PFUs of SINV (TE12-untagged) containing citrated rabbit blood (Fisher Scientific DRB030) supplemented with 1mM ATP (VWR ...
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bioRxiv - Microbiology 2020Quote: ... 30,000 cells/well were cultured at 37°C in 5% CO2 as a monolayer on coverslips (d = 12 mm; Paul Marienfeld GmbH & Co. KG) in 24 well plates (#08-772-1H, Thermo Fisher Scientific) with DMEM ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were blocked in blocking buffer (1% BSA, PBST) for 1h at room temperature and incubated with primary antibody LC3A/B (Thermo Scientific, USA) overnight at 4 °C ...
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bioRxiv - Bioengineering 2021Quote: ... Washed samples were incubated for 1h at RT with the secondary antibody (1:200 Alexa Fluor 647 goat derived anti-rabbit antibody, Thermo Fisher Scientific). Then ...
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bioRxiv - Neuroscience 2020Quote: ... for 2h at RT followed by a 1h incubation with 1:200 secondary goat anti-rabbit antibody Alexa 647 (Thermo Fisher, A32733) and DAPI to visualize cell nuclei ...
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bioRxiv - Microbiology 2021Quote: ... Cells infected with YFV were incubated with the pan-flavivirus anti-Env 4G2 antibody for 1h at 4°C and then with Alexa 488 anti-mouse IgG secondary antibodies (Thermo Fisher, #A28175) for 45 min at 4°C in the dark ...
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bioRxiv - Cancer Biology 2020Quote: ... the flasks were shaken at 37°C for 1h at 170 rpm in an orbital shaker (Max Q 4000; Thermo Fisher Scientific). The supernatant containing microglia was then collected [36].
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bioRxiv - Neuroscience 2020Quote: ... for 1h at room temperature (or overnight at 4°C) and the unbound dye was removed using spin columns (Thermofisher, Waltham, MA). The labeling efficiency was estimated using absorbance values (488 or 555 or 660 nm/280 nm ...
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bioRxiv - Microbiology 2022Quote: ... Bound primary antibody was then labeled for 1h at room temperature using Alexa488 goat anti-mouse IgG secondary antibody (Invitrogen, OR, USA) at a 1:750 dilution in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... samples were washed three times and incubated 1h at room temperature with secondary antibodies: Alexa Fluor Plus 488 goat anti-Rabbit (A32731, Thermo Fisher Scientific) and Alexa Fluor Plus 546 donkey anti-goat (A11056 ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were washed and incubated for 1h with Alexa Fluor 488-conjuated antibody to rabbit IgG (A-21206, 1:100, Thermo fisher scientific). Images of green fluorescence were assessed by laser confocal fluorescence microscopy.
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bioRxiv - Microbiology 2019Quote: ... pneumophila antisera (1:20,000) for 1h at 37°C in PBS containing 4% BSA followed by anti-rabbit IgG Alex Fluor 488 (Invitrogen, 1:500) for 1h at 37°C in PBS containing 4% BSA ...
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bioRxiv - Microbiology 2021Quote: ... The beta-propiolactone was inactivated by incubation at 37C for 2 hours and the inactivated virions were pelleted by ultracentrifugation at 25000g for 1h at 40C and protein concentration was determined by BCA assay (Thermo Fisher Scientific).
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Overexpression of HLA class I predominantly on alpha cell in at risk individuals for type 1 diabetesbioRxiv - Immunology 2020Quote: ... for 1h at room temperature (RT) followed by detection using polyclonal goat anti-mouse IgG-Alexa Fluor 647 (Life Technologies, 1:1000) for 30 min at RT ...
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bioRxiv - Developmental Biology 2022Quote: ... Maturation of released oocytes was induced by incubating for 1h at 16C in 3 μM 1-Methyladenine (Fisher Scientific, 5142-22-3). All embryos were raised in 0.22 μm filtered sea water (FSW ...
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bioRxiv - Cancer Biology 2022Quote: ... in the hybridization buffer for 1h at RTThe sections were washed with PBS and mounted with SlowFade Gold Antifade Mountant (Thermo Fisher Scientific). After each imaging cycle ...
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bioRxiv - Neuroscience 2022Quote: ... Protein lysates were pre-cleared 1h at 4°C on a rotating wheel with 50μl of previously washed Dynabeads Protein G (Invitrogen, Thermo Fisher Scientific), to avoid aspecific bindings ...
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bioRxiv - Cancer Biology 2022Quote: ... diluted at 1/50 or 1/150 followed by an incubation for 1h at room temperature with a secondary goat Alexa FluorTM 488 antibody (ThermoFisher Scientific, #A11029) diluted at 1/1000 ...
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bioRxiv - Neuroscience 2023Quote: ... 10% Donkey Serum, 1% Triton-100, 1h) followed by primary (overnight, 4°C) and secondary antibodies (1-2h, Jackson ImmunoResearch or ThermoFisher Scientific) incubation ...
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bioRxiv - Cell Biology 2023Quote: ... primary antibodies in 10% GS in 1xPBS overnight at 4°C followed by a 1h incubation with anti-mouse (A11003, Invitrogen, 1:800) secondary antibody in 10% GS in 1xPBS at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibody incubation with anti-rabbit-HRP and anti-mouse-HRP was done at room temperature for 1h in 5% milk in TBS-T and the signal developed using an ECL mixture (Life Technologies, 32132). For a detailed list of antibodies see Supplementary Table 2 ...
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bioRxiv - Genetics 2023Quote: ... 1 μL was spotted onto a C+Y-10% polyacrylamide pad (incubated twice for 1h in C+Y medium supplemented with daptomycin when applicable) inside a Gene Frame (Thermo Fisher Scientific) sealed with a cover glass ...
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bioRxiv - Biochemistry 2023Quote: ... the slides were incubated with primary antibodies in PBS containing 5% BSA for 2 h and then with the following secondary antibodies for 1h at room temperature: Donkey Anti-Rabbit Alexa 488 (1:1000; Invitrogen; A21206, 2376850) and Donkey Anti-Chicken Alexa 594 (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: 250’000 Jurkat cells or purified T cells were seeded onto a 24-well plate and transduced at an MOI of 5 via spin-infection for 1h at 800xg and 32°C in 450 µl RPMI-1640 medium (Gibco, #42401-018) containing 50 µl of 100-fold concentrated lentiviruses ...
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bioRxiv - Microbiology 2023Quote: ... The cell membrane fraction was isolated from the lysates by performing ultracentrifugation at 66000g for 1h (Sorvall WX Ultra 80 Centrifuge, Thermo Fisher Scientific). After resuspending the obtained pellet with PBS ...
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bioRxiv - Bioengineering 2023Quote: ... stained with primary antibody for 1h at 37° C and labeled against F-actin (1:60 in PBS Alexa Fluor® 488 Phalloidin-Invitrogen, # A12379) and DNA (NucBlue ...
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bioRxiv - Microbiology 2024Quote: ... then the following secondary antibodies were diluted 1:500 in blocking buffer and incubated for 1h with the cells: anti-rabbit DyLight 448 (Thermo Scientific, 35552), anti-rabbit DyLight 550 (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... and incubated 1h at RT in the dark with a secondary antibody solution containing goat anti-mouse Alexa 594 (A11032, Invitrogen, Fisher Scientific) fluorescent-coupled antibody diluted to 1:1000 in PBS-T solution ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Nvsix3/6:venus was generated by subcloning the Nvsix3/6 coding sequence into pENTR/D TOPO (ThermoFisher Scientific) using published primers previously used to PCR amplify Nvsix3/6 and synthesize Nvsix3/6 mRNA 47 ...
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bioRxiv - Microbiology 2021Quote: ... thaliana seedlings cultured in 6 ml liquid MSgg of each well of a 6-well microplate (Thermo Scientific). 8 seedlings were put in each well ...
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bioRxiv - Microbiology 2020Quote: ... Cells in antibiotic free media (8×105/6-well) were transfected 6 hours post infection with 2μg plasmid and 6μl Lipofectamine 2000 (Invitrogen) per well diluted in Opti-MEM (Invitrogen).
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bioRxiv - Neuroscience 2021Quote: ... with Essential 6 medium (#A1516401; Life Technologies) containing dorsomorphin (2.5 μM ...
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bioRxiv - Molecular Biology 2020Quote: ... Twelve-well Novex 6% Trisglycine gels (Invitrogen) were pre-run in 0.5× Tris-Borate-EDTA (TBE ...
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bioRxiv - Genomics 2020Quote: ... on the QuantStudio 6 Flex (Life Technologies). Next ...
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bioRxiv - Immunology 2021Quote: ... IL-6 mouse ELISA kit (ThermoFisher Scientific) and IL-1β mouse ELISA kit (ThermoFisher Scientific) ...
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bioRxiv - Genetics 2020Quote: ... or the QuantStudio 6 Flex (Thermo Fisher). Samples were analyzed using a two-step amplification and melt curves were obtained after 40 cycles ...
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bioRxiv - Genetics 2020Quote: ... or the QuantStudio 6 Flex (Thermo Fisher). The Ct values were analyzed by the enrichment compared to input method.
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bioRxiv - Bioengineering 2019Quote: ... Novex TBE - DNA retardation gels (6%) – (ThermoFisher) were used for the electrophoresis.
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bioRxiv - Neuroscience 2019Quote: ... in 6-well culture plates (ThermoFisher Scientific) with 800 µL of sterile medium added below the insert (Stoppini et al. ...
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bioRxiv - Neuroscience 2020Quote: ... with 6 μl of RNaseout (Life Technologies). The lysates were sequentially treated with 12.6 μl of RNase-free DNase (Promega ...
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bioRxiv - Microbiology 2021Quote: ... QuantStudio 6/7 Pro systems (Applied Biosystems). The following primers were used ...
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bioRxiv - Neuroscience 2019Quote: ... on a QuantStudio 6 (ThermoFisher, Waltham, MA) real-time PCR machine ...
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bioRxiv - Microbiology 2021Quote: ... in a QuantStudio 6 thermocycler (Applied Biosystems) or in a StepOne Plus thermocycler (Applied Biosystems) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 6 ug/mL Puromycin (Gibco #A1113803).
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bioRxiv - Cell Biology 2021Quote: ... 12 (Fisher Scientific DF2948-47-6; RRID:AB_2884995); Mouse mAb anti-Cdc42 (BD Biosciences 610928 ...