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Citations for Thermo Fisher :
1201 - 1250 of 10000+ citations for 6 THIOPHEN 2 YL 1H INDAZOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... and the cells were serum-starved for 1h by adding 1.5mL Opti-MEM® reduced-serum medium (Gibco, Life Technologies) per well prior to transfection ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed 3 times with washing buffer (1x PBS and 0.025% of Tween20) for 5 min and incubated for 1h at RT with the Alexa Fluor secondary antibodies (Thermo Scientific). Cells were washed twice with washing buffer for 5 min and incubated in with DAPI (1 μg/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... tissues were incubated with a species-specific secondary antibody (Alexa 647) for 1h then washed 3 times with PBS and mounted using ProLong Gold Antifade Mountant with DAPI (Invitrogen).
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bioRxiv - Developmental Biology 2020Quote: ... Dissociated murine prostate cells were obtained by digesting pre-minced prostate tissue for 1h at 37°C in digestive medium prepared in prepared in ADMEM/F12 (Gibco), and containing 1mg/mL Collagenase Type I (ThermoFischer Scientific ...
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bioRxiv - Molecular Biology 2021Quote: J1 mES cells were grown on glass coverslips and incubated for 1h (37°C, 5% CO2) with EU from the Click-iT RNA Alexa Fluor 594 imaging kit (C10330, Invitrogen). Cells were fixed with 3,7% formaldehyde in PBS 1× for 15min at room temperature and permeabilized with 0.5% Triton X-100 in PBS 1× for 15min at room temperature ...
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bioRxiv - Genomics 2020Quote: ... W4021) were added for 1h and protein signals were detected using Pierce™ ECL Western Blotting Substrate kit (ThermoFisher 32109), using a Fujifilm LAS4000 luminescence imager and band intensities quantified using ImageJ ...
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bioRxiv - Cell Biology 2019Quote: ... Permeabilized cells were then incubated with anti-megalin and anti-FLAG antibodies at 4°C for 1h in the presence of 0.05% saponin followed by incubation with Alexa 488 or Alexa 647 secondary antibodies (Molecular Probes) at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... burnetii (1:2500; Robert Heinzen, NIH, Hamilton, MT) for 1h followed by Alexa fluor secondary antibodies (1:1000; Life Technologies) for 1 h ...
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bioRxiv - Cancer Biology 2019Quote: ... Blocking was done in 3% BSA in PBST 0.5% for 1h at room temperature followed by EdU detection with the Click-iT EdU reaction kit (Thermo Scientific), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... slides were rinsed with PBS and incubated for 1h with secondary antibodies (Alexa Fluor 488 anti-rabbit and Alexa Fluor 568 anti-rat; Invitrogen) and DAPI (4083 ...
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bioRxiv - Microbiology 2021Quote: ... Following another 3 washes with PBS plates were incubated for 1h with secondary goat anti-guinea pig-AlexaFluor488 (Invitrogen, UK) at 1:500 in PBS-0.5%BSA ...
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bioRxiv - Microbiology 2020Quote: ... The non-encapsidated DNA was then removed by a 1h incubation at 37°C with 4 U TURBO DNase enzyme (Invitrogen), 1X TURBO DNase Buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... washed thrice with PBST followed by 1h incubation with Alexa fluor conjugated secondary antibody (Thermo Fisher Scientific Cat # 11008, 21244) then again washed thrice with 1XPBST ...
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bioRxiv - Immunology 2022Quote: ... were generated by plastic adherence for 1h in complete RPMI 1640 (680 μM L-Glutamine and 10 mM Hepes, GIBCO) supplemented with 5% pooled human serum (The Blood Bank ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then incubated for 1h in goat-anti-rabbit AlexaFluor 488 secondary antibody (1:200; #A11034, ThermoFisher Scientific, MA), followed by 5x PBS washes ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated at 25°C for 1h in a solution containing 0.1% Triton X and Hoechst 33342 (1:5000, Invitrogen, H3570). Sections were mounted with Fluoromount-G (Southern Biotech ...
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bioRxiv - Cell Biology 2021Quote: ... samples were incubated at room temperature for 1h with the corresponding fluorescence secondary antibodies conjugated to Alexa Fluor 488 or 555 (Invitrogen) at 1:400 ...
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bioRxiv - Bioengineering 2021Quote: ... Chips were then incubated at 37°C for ~1h until cells were fully dissociated into single cells at which point DTI (Gibco) was added to neutralize the enzymatic activity ...
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Activation of endoplasmic reticulum stress via clustering of the inner nuclear membrane protein SUN2bioRxiv - Cell Biology 2022Quote: ... were coated for 1h with 0.1% gelatin solution (ATCC, PCS-999-027) and washed twice with PBS (pH 7.4, Thermo Fisher Scientific). HEK293FT cells were plated on the gelatin coated dish and grown overnight in normal growth medium to reach ∼ 70% confluency ...
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bioRxiv - Physiology 2019Quote: ... Primary antibody was detected by a 1h incubation with AlexaFluor-conjugated goat anti-rabbit secondary antibody (Life Technologies, A-11072) diluted in blocking buffer ...
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bioRxiv - Cell Biology 2019Quote: ... cells were incubated for 1h with a secondary antibody: Alexa-488nm donkey anti-rat or goat anti-mouse or goat anti-rabbit (Invitrogen) (dilution 1/400) ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were washed in TBST buffer prior to incubation with secondary fluorophore conjugated antibodies 1:2000 for 1h at RT (goat anti-mouse DyLight 680 Invitrogen A21058 ...
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bioRxiv - Microbiology 2020Quote: ... 0.05% saponin at room temperature for 1h followed by Alexa FluorTM 647 goat anti-rabbit IgG (ThermoFisher A21245; 1:1000), 0.05% saponin at room temperature for 1h followed by nuclear staining with Hoechst 33342 (Thermo Fisher Scientific 62249 ...
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bioRxiv - Cancer Biology 2021Quote: ... for 2 hours in the dark at RT followed by 1h incubation with secondary antibodies: anti–mouse Alexa Fluor 488 (1:300, A11001, Invitrogen) and anti–rat Cy3 (1:150 ...
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bioRxiv - Microbiology 2020Quote: ... 0.05% saponin at room temperature for 1h followed by nuclear staining with Hoechst 33342 (Thermo Fisher Scientific 62249; 1:2000) for 15 min ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated with primary antibodies in 10% NGS/NDS in PBST over night at 4°C following 1h incubation at 24°C with appropriate Alexa488/568 coupled secondary antibodies (1:1000, Invitrogen). Cell nuclei were stained with DAPI and final images were acquired using Leica TCS SP8 confocal microscope (Leica Microsystems ...
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bioRxiv - Immunology 2021Quote: from PyMT or wild-type littermate control were cut in small pieces and digested for 1h at 37°C in CO2-independent medium (Gibco) containing 150 μg/mL DNAse and 75 μg/mL Liberase TL (Roche) ...
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bioRxiv - Cancer Biology 2021Quote: ... the slides were washed 3 times with PBS and incubated for 1h at room temperature with goat anti-rabbit AlexaFluor-568 (ThermoFisher, Invitrogen A-1101 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were put back on ice and treated for 1h with anti-Alexa fluor® 488 (#A11094 from Life Technologies) in order to quench remaining surface signal ...
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bioRxiv - Cancer Biology 2021Quote: ... and the cells were serum-starved for 1h by adding 1.5mL Opti-MEM® reduced-serum medium (Gibco, Life Technologies) per well prior to transfection ...
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bioRxiv - Biophysics 2020Quote: ... fixed cells are incubated 1h at room temperature with Myosin 4 Monoclonal Antibody conjugated with Alexa Fluor 488 (MF20, Thermofisher) at 5μg/mL (1:100 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... The sections were blocked in blocking buffer for 1h at room temperature and incubated with BODIPY 558/568 (Invitrogen, D38D35) for 20 minutes ...
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bioRxiv - Plant Biology 2022Quote: ... and immunocomplexes were next incubated for 1h at 4°C with 40 μL of Dynabeads Protein A (Thermo Fisher Scientific). The beads were washed 2 × 5 min in ChIP Wash Buffer 1 (0.1% SDS ...
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bioRxiv - Molecular Biology 2022Quote: ... were incubated for 1h at room temperature in PBS- tween with 2.5% BSA and developed using ECL Western blot reagent (Thermo Scientific).
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bioRxiv - Molecular Biology 2022Quote: ... coverslips were incubated for 1h at room temperature with goat-anti-rabbit Alexa Fluor™ 488 (1:500, ThermoFisher Scientific) as a secondary antibody ...
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bioRxiv - Cell Biology 2023Quote: ... the iMACs were washed 3 x 5 min with PBS and incubated for 1h with 1:1000 goat anti-mouse IgG-AF488 (A11001, Invitrogen). Afterwards the cells were again washed 3 x 5 min with PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were subsequently rinsed and incubated for 1h at RT with the appropriate HorseRadish-Peroxidase (HRP)-conjugated secondary antibodies (Invitrogen). The visualisation of the signal was conducted using Immobilon™ Forte Western HRP Substrate (Millipore ...
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bioRxiv - Immunology 2023Quote: ... 100 uL ofmedium from the bottom chamber were collected at either 1h or 4h after Dextran addiction and fluorescence was quantified (Emission; 490, Absorption; 520) on black 96 multiwell plates (Nunc) on CLARIOstar OPTIMA (BMG LabTech ...
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bioRxiv - Immunology 2023Quote: ... The slides were then rinsed 4 times with the washing buffer for 15 minutes and incubated with the secondary antibody at 1:500 dilution in blocking solution for 1h at room temperature (Rabbit 555, Invitrogen, #AB_162543 ...
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bioRxiv - Biophysics 2022Quote: ... The ligation was desalted by dialysis using membrane filters for 1h and later concentrated 3.3X using a SpeedVac concentrator (Thermo Scientific).
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bioRxiv - Immunology 2023Quote: ... Blocking was 1h in PBS + 5% w/v bovine serum albumin (BSA) and 2.5 µg/mL anti-CD16-CD32 (FcBlock, Thermo Fisher). Primary antibodies (Table 1 ...
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bioRxiv - Zoology 2024Quote: ... Excreta were then collected over intervals of 1h-1h30 in 500 µl of DMEM containing 1% antibiotic-antimycotic (Gibco, France). Before adding media ...
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bioRxiv - Bioengineering 2023Quote: ... and 0.3% Triton-X solution in PBS for 1h at room temperature and then incubated with a primary rabbit anti-RLuc antibody (1:1000, PA1-180, ThermoFisher) in blocking buffer for overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... sections were boiled in sodium citrate buffer (pH 6.0) for 15 min and incubated with the following antibodies for 1h: anti-CD8 (4SM15, Thermo Scientific), anti-NCR1 (EPR23097-35 ...
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bioRxiv - Immunology 2023Quote: ... Coverslips were then incubated for 1h at RT with the appropriate Alexa Fluor conjugated secondary antibody (Invitrogen, 1:300 dilution), incubated for 10 min with DAPI at 1μg/mL in PBS and mounted on slides using Dako Fluorescence Mounting Media.
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bioRxiv - Systems Biology 2023Quote: ... samples were incubated for 1h with a goat anti-guinea pig secondary antibody (Alexa Fluor 488 conjugate; 1:1000; Invitrogen) and wheat germ agglutinin to label the nuclear envelope (Alexa Fluor 647 conjugate ...
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bioRxiv - Microbiology 2024Quote: ... The slides were incubated for 1h at RT with primary antibody (1:20) Mouse Monoclonal Ab anti-AID (ZA001, Invitrogen), in PBS-0.1% BSA-0.4% Tween20 ...
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bioRxiv - Microbiology 2019Quote: ... ATPase was assayed by a continuous spectrophotometric method using a 2-amino-6-mercapto-7-methylpurine ribonucleoside/purine nucleoside phosphorylase reaction to detect the released inorganic phosphate (EnzChek Kit; Life Technologies, California, USA) (53) ...
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bioRxiv - Cell Biology 2020Quote: 2 × 105 cells were transfected in a gelatin-coated 6-well plate in ES DMEM-2i using Lipofectamine 2000 (Fisher Scientific, 11668-027). Two pairs of gRNA were used for each gene (1 µg each ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific) as counterstaining.