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Citations for Thermo Fisher :
1401 - 1450 of 10000+ citations for 4 Chloro 6 fluorobenzene 1 3 diamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: mRNA was collected from groups of 10 whole larvae (n=3–4 replicates per line) using Trizol (ThermoFisher) and reverse transcribed to cDNA using SuperScript III Reverse Transcriptase (ThermoFisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... and Antibiotic-Antimycotic Mixed Stock Solution (Nacalai) and were passaged every 3-4 days with TryPLE (ThermoFisher Scientific). For microscopy observations ...
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bioRxiv - Bioengineering 2020Quote: ... Bodipy FL c16 (4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Hexadecanoic Acid, Thermofisher) was diluted in sterile RPMI-1640 (Corning ...
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bioRxiv - Molecular Biology 2020Quote: ... a sample of eluates (3%) was separated on acrylamide NuPAGE Novex 4–12% Bis-Tris gels (Life Technologies) and analyzed by silver staining.
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bioRxiv - Biochemistry 2021Quote: ... and then diluted into fresh YPD for 3-4 hours before inoculating into 96-well plates (Thermo Scientific) at a starting OD600 between 0.04 to 0.1 ...
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bioRxiv - Biochemistry 2022Quote: ... and then analysed on Novex WedgeWell 4-12 % Tris-Glycine Gels or 3-8 % Tris-Acetate gels (Invitrogen). Tris-Glycine gels were run at 180 V for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... and a lentiviral transfer plasmid (2:3:4 ratio by mass) using Lipofectamine 3000 (Thermo Fisher Scientific L3000015). Viral supernatant was harvested 48h after transfection and filtered through 0.45 µm cellulose acetate filters (Corning 431220) ...
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bioRxiv - Cancer Biology 2024Quote: ... at 95⁰C for 3 min and loaded onto a SDS-polyacrylamide gel (4-12%, Invitrogen NuPAGE, #NP0336BOX). After size separation ...
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bioRxiv - Biochemistry 2023Quote: ... the filtered supernatant was loaded onto 3-4 mL immobilized D-galactose gel (Pierce™, Thermo Scientific™) equilibrated by gravity flow with Gal A buffer (50 mM Na-phosphate pH 7.4 ...
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bioRxiv - Immunology 2024Quote: 50 000 enriched CD11b+ LCs were seeded and incubated for 10min at 37°C with 2mM Bodipy-C11 (581/591) (4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid; Invitrogen) in PBS ...
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bioRxiv - Genomics 2024Quote: ... The remaining cells are resuspended in our working cell buffer solution (AMES/0.4% BSA + 3 µM ActD) and incubated with CD90.2/Thy1.2 antibody conjugated to APC (#17-0902-83; Invitrogen, Carlsbad ...
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bioRxiv - Molecular Biology 2024Quote: ... the cells were cultured for 3-4 days in phenol red-free DMEM/F-12 (Thermo Fisher 11039021) supplemented with 5% charcoal-dextran-treated FBS ...
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bioRxiv - Bioengineering 2024Quote: ... VSV-G expression plasmid and pUC19 plasmid (2:3:4 ratio by mass) using Lipofectamine 3000 (Thermo Fisher). The media was changed 6 hours post-transfection and was collected for downstream processing 48 hours post-transfection.
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bioRxiv - Molecular Biology 2024Quote: ... protein samples were resolved by SDS-PAGE on pre-cast NuPAGE™ gels (1.0 mm 4–12% Bis-Tris or 1.5 mm 3–8% Tris-Acetate for HMW TRIM37, Invitrogen) with molecular weight ladders (PageRuler Plus or HiMark pre-stained protein standard for HMW TRIM37 ...
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bioRxiv - Developmental Biology 2020Quote: ... Chromatin was stained with To-Pro-3 (1/200 dilution, 1 mM, Invitrogen T3605).
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bioRxiv - Neuroscience 2022Quote: ... dried and coverslipped under glycerol:TBS (3:1) with Hoechst 33342 (1:1000; ThermoFisher Scientific). Primary antibodies with the following dilutions used in this study include ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibody (2-3% serum, 0.4% PBST, 1:500 Invitrogen A11035, 1:500 DAPI) was applied and incubated for one hour at room temperature ...
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bioRxiv - Pathology 2024Quote: ... and 3-hydroxybutyrate dehydrogenase 1 (15417-1-AP, Thermo Fisher Sci, Waltham, MA, USA). HRP-conjugated secondary antibodies (NA9340 ...
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bioRxiv - Biochemistry 2024Quote: ... columns were washed with 3 column volumes (CV) of 1:1 RNase ZAP (Ambion) followed by 3 CV nuclease-free water (DEPC-treated MilliQ water) ...
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bioRxiv - Microbiology 2020Quote: ... 100μm lung slices were washed twice in 200 mM HEPES buffer then incubated for 20 min in a staining solution containing: 0.715 μM DAPI (4′,6-diamidino-2-phenylindole) (ThermoFisher Scientific D1306), 10 mg/L BODIPY 493/503 (4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 and 6 were directly cloned into pCR(tm)2.1-TOPO® using the TOPO® TA Cloning® Kit (Invitrogen) following manufacturer’s instructions ...
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bioRxiv - Zoology 2020Quote: ... Tissues were cut into small pieces with a scalpel and washed twice in ADPBS containing antibiotics prior to placing 4-6 tissue pieces into a 25 cm2 plug-seal tissue culture treated flask (BioLite, Thermo Scientific) containing 1.5 mL establishment medium to cover the tissue pieces ...
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bioRxiv - Biophysics 2021Quote: ... cells were transiently transfected with 4 μg mouse Piezo1-pIRES-EGFP in pcDNA3.1(+) or 4 μg YFP 40-48 hours before recording in 6-well plates using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... 4 µg of plasmid was transfected with 4 µL P3000 regent and 6 µL Lipofectamine 3000 as per scaled recommendations in the manufacturer’s protocol (Thermo Fisher Scientific). Medium containing lipid complexes was removed 4 h post transfection and replaced with exosome-depleted phenol red-free DMEM + 10% FBS + 2 mM L-glutamine (Thermo Fisher) ...
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bioRxiv - Bioengineering 2021Quote: ... 4 µg of plasmid was transfected with 4 µL P3000 regent and 6 µL Lipofectamine 3000 as per scaled recommendations in the manufacturer’s protocol (Thermo Fisher Scientific). Medium containing lipid complexes was removed 4 h post transfection and replaced with exosome-depleted phenol-free DMEM + 10% FBS + 2 mM L-glutamine ...
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bioRxiv - Bioengineering 2021Quote: ... and the cells were incubated for 50 minutes and DAPI (4′,6′-diamino-2-phenylindole) (Molecular Probes, Thermo Fisher Scientific, USA) for 5 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... and the cells were incubated for 50 minutes and DAPI (4′,6′-diamino-2-phenylindole) (Molecular Probes, Thermo Fisher Scientific, USA) for 5 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... slides were washed 3 x 5 min with PBS-TX and once with 0.1 M phosphate buffer pH 7.4 (0.08 M sodium phosphate dibasic and 0.02 M sodium phosphate monobasic) prior to coverslipping with ProLong Gold antifade reagent with 4’,6-diamidino-2-phenylindole [DAPI] (Invitrogen, P36931).
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bioRxiv - Neuroscience 2021Quote: Hair cell nuclei were specifically labeled by briefly exposing free-swimming larvae to 4’,6-Diamidino-2-Phenylindole (DAPI; Invitrogen/ThermoFisher) diluted to 2.5 μg/ml in E3 ...
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bioRxiv - Genetics 2021Quote: ... cells were washed and suspended in flow cytometry buffer containing 1µg/ml 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2020Quote: ... Sections were cut at 4-6 μm and dried from a drop of dH2O onto Probe On Plus slides (ThermoFisher Scientific). Tissues sections were stained with 0.01% aqueous Toluidine Blue O ...
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bioRxiv - Molecular Biology 2020Quote: ... once with 1X SSC at room temperature for 15 min and twice with 0.5X SSC at room temperature for 15 min and finally with 1X PBS buffer containing 0.5 µg/mL 4′,6-diamidino-2-phenylindole (DAPI) and mounted onto ProLong Gold antifade reagent (Thermo Fisher Scientific) mounting media.
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bioRxiv - Cell Biology 2022Quote: ... the cerebral cortices of 4 to 6 P0 rats were dissociated and chopped in Ca++/Mg++-free Hank’s Balanced Salt Solution (HBSS; Invitrogen, Carlsbad, CA). The tissue pieces were dissociated in phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2022Quote: ... diluted 1:200 in blocking buffer for 30 min and mounted with ProLong Gold Antifade with DAPI (4′,6-diamidino-2- phenylindole; Thermo Fisher). Confocal laser micrographs were obtained with Zeiss LSM 880 laser microscope and analyzed with Zen black and Fiji software ...
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bioRxiv - Biophysics 2022Quote: ... gels were functionalized with 0.5 mg/ml solution of sulfosuccinimidyl 6-(4′-azido-2′-nitrophenylamino) hexanoate (Sulfo-SANPAH) (Thermo Fisher Scientific) prepared in 50 mM HEPES buffer (Santa Cruz Biotechnologies) ...
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bioRxiv - Microbiology 2020Quote: ... cells were extensively washed and incubated with secondary antibodies in the presence of 4′,6-diamidino-2-phenylindole (DAPI, Molecular Probes) for 45 min at RT ...
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bioRxiv - Developmental Biology 2021Quote: ... transferred to 1.6 mL Eppendorf tubes and spun at 2000 rcf for 5 minutes at 4°C in an angle rotor (Thermo Scientific Fisher Heraeus Biofuge Primo R Centrifuge) ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed and mounted in media (0.5% N propyl gallate, 70% glycerol, 1M tris pH8.0) containing 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Cat. No. D1306).
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bioRxiv - Microbiology 2022Quote: ... After extensive washing slides were air-dried and mounted in Gold Antifade containing 4’,6-diamidino-2-phenylindole (DAPI) (P3693; Life Technologies) for imaging ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed in PBS-T and mounted under Prolong gold fluorescence media with 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen, P36931).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Alexa Fluor 488–conjugated phalloidin (#A12379) and 4’,6-diamidino-2-phenylindole (DAPI) (#1306) were obtained from Invitrogen (Carlsbad, CA, USA). IRDye800CW-labeled anti-rabbit secondary antibody (#926-32211 ...
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bioRxiv - Neuroscience 2020Quote: ... slides were washed and stained with 4′,6-Diamidin-2-phenylindol (DAPI, 5μg/ml) before mounting coverslips with ProlongTM Gold Antifade reagent (Thermo Fisher Scientific). Images were acquired using a LSM 710 confocal microscope (Zeiss ...
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bioRxiv - Microbiology 2021Quote: ... samples were unfrozen and aliquots of 6 ml per sample were fixed with Electron Microscopy Sciences 4% Paraformaldehyde (5025999, Fisher Scientific), stained with SYBR™ Gold Nucleic Acid Gel Stain (S11494 ...
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bioRxiv - Immunology 2021Quote: ... The isolated nuclei were centrifuged at 500 x g for 5 min at 4°C and respectively resuspended with Nuclei wash buffer stained with 10 µg/ml 4′,6-diamidino-2-phenylindole (DAPI) before FACS (5mg/ml, Invitrogen D1306). After sorting using purity mode ...
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bioRxiv - Biochemistry 2020Quote: ... 500 mM 6-aminohexanoic acid) was added to lysates right before loading samples into a NativePAGE Bis-Tris gel 4-16% (Life Technologies). The proteins were separated according to Wittig et al.32 and transferred onto a PVDF membrane using a standard Tris-glycine transfer buffer with 0.05% SDS ...
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bioRxiv - Biochemistry 2020Quote: ... Nuclei were stained with 10.9 μΜ 4’,6-diamidino-2-phenylindole (DAPI) and cover glasses were mounted in ProlongGold (Life Technologies). Cells were incubated 3x for 5 min in blocking solution to remove unbound antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then dipped in the secondary antibody-containing buffer with the nuclear dye 4′,6-diamidino-2-phenylindole (DAPI, Cat# D1306, Life Technologies-Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclear staining of glass-mounted brain sections was performed by 10 min incubation with PBS containing 2.5% DAPI (4’,6-diamidino-2-phenylindole, Thermo Fisher Scientific). Sections were embedded in mounting medium containing 1,4-diazabicyclooctane (DABCO ...
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bioRxiv - Developmental Biology 2024Quote: ... and counterstained with 5 μg/mL of 4′,6-diamidino-2-phenylin-dole (DAPI) or propidium iodide (Molecular Probes, Life Technologies) for 10 min ...
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bioRxiv - Developmental Biology 2024Quote: ... and counterstained with 5 μg/mL of 4′,6-diamidino-2-phenylin-dole (DAPI) or propidium iodide (Molecular Probes, Life Technologies) for 10 min ...