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Citations for Thermo Fisher :
1201 - 1250 of 10000+ citations for 4 Chloro 6 fluorobenzene 1 3 diamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... Cleaved Caspase-3 (Asp175) (1:200, Invitrogen PA5-114687), Tom20 (1:400 ...
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bioRxiv - Microbiology 2024Quote: ... 1% P/S and 3 μg/ml puromycin (Gibco). Human foreskin fibroblasts (HFFs ...
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bioRxiv - Neuroscience 2024Quote: ... 8) anti-GFAP (1/250) (Thermofisher scientific, 3-0300), 9 ...
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bioRxiv - Cancer Biology 2024Quote: ... or TO-PRO-3 Stain (1 µM, ThermoFisher Scientific). Flow cytometry was performed on either an LSRII ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... with 4 μg of a plasmid mixture (1:1:4 THR:RXR:TRE-Luc) in 200 μL Opti-MEM (Invitrogen, 11058-021) and added to the cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were also stained for 30 minutes simultaneously with 1 μM 2′-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]−2,5′-bi-1H-benzimidazoletrihydrochloride trihydrate (Hoechst 33342, Fisher Scientific) for nuclear visualization and cell localization ...
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bioRxiv - Cell Biology 2020Quote: ... Adult cardiac NMCs were isolated from adult C56BL/6 mice (6-8 weeks old) by digesting adult ventricles in buffer containing 1 mg/ml collagenase IV (Gibco 17104-019) and 1.2 mg/ml dispase II (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... NIM was exchanged daily for 3:1-DMEM/F12-medium (3 parts DMEM (Thermo Fisher Scientific, #31966047) and one-part F12 medium (Ham’s F-12 Nutrient Mix ...
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bioRxiv - Microbiology 2021Quote: ... 6- diamidino-2-phenylindole dihydrochloride (DAPI; Thermo Fisher D1306; 1:1,000 dilution) for 20 min ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti phospho-tau PHF-6 (Thr231) (1:1000, Thermofisher #35-5200); mouse anti-β-Actin (1:20000 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were passaged 1:6 once at 70% confluency using Versene (Gibco). Monocyte factories were set up following a previously reported protocol (van Wilgenburg et al. ...
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bioRxiv - Neuroscience 2023Quote: ... diluted 1:6 in DMEM/F12 media (11320033, Thermo Fisher, Waltham, USA). The iPSCs were maintained ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were passaged 1:6 once at 70% confluency using Versene (Gibco). Monocyte factories were set up following a previously reported protocol (van Wilgenburg et al. ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining material was resuspended in 6 mL sterile 1× PBS (Gibco). Samples were ultracentrifuged at 4 °C and 150,000 ˗ g for 2 h (Optima L-80 XP Ultracentrifuge with Type 70.1 Ti Fixed-Angle Titanium Rotor (Beckman Coulter) ...
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bioRxiv - Bioengineering 2024Quote: ... 6-diamidino-2 phenylindol (DAPI, 1 ug/mL; Thermo Fisher, Waltham, MA), and AlexaFluor 488 phalloidin (1:100 ...
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bioRxiv - Biochemistry 2020Quote: ... and 4 μg ml−1 Blasticidin (Thermo Fisher), single cell colonies were collected and subsequently expanded ...
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bioRxiv - Developmental Biology 2022Quote: ... anti-Ki67 (ThermoFisher, 4-5698-82, 1:100), anti-Actinin (Cell Signaling ...
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bioRxiv - Bioengineering 2021Quote: ... 1-2 drops of CytoSeal (Thermofisher, 8312-4) were placed on each before mounting a coverslip ...
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bioRxiv - Cell Biology 2022Quote: ... ERCC 1:4 × 107 dilution (Ambion, cat: 4456740) in 384-well plates prior to library preparation.
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bioRxiv - Genomics 2021Quote: ... 1 mL of hexane (Fisher Scientific; H303-4) followed by 1 mL of deionized water was added to each sample ...
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bioRxiv - Genomics 2023Quote: ... 4 µL DNase I (ThermoFisher, 1 unit/µL), 4 µL RNase A (ThermoFisher ...
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bioRxiv - Bioengineering 2024Quote: ... and 4 μM ethidum homodimer-1 (Invitrogen™) in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... 4 μM ethidium homodimer-1 (EthD1, ThermoFisher, E1169), and 0.02 mg/ml deoxyribonuclease I ...
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bioRxiv - Bioengineering 2024Quote: ... and 4 μM Ethidum homodimer-1 (Invitrogen™) in PBS for 30 min at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-spectrin beta-4 (1:100, Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... diluted 1:4 in 1X DPBS (ThermoFisher, 14190144), 0.05 μL of 40 μM ASO diluted in 4.165 μL Opti-MEM was combined with 4.165 μL Lipofectamine RNAiMAX diluted in 0.25 μL Opti-MEM ...
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bioRxiv - Bioengineering 2024Quote: ... anti-claudin 4 (1:100, Invitrogen 36-4800), anti-laminin (1:250 ...
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bioRxiv - Cell Biology 2024Quote: ... diluted 1:4 in 1X DPBS (ThermoFisher, 14190144). All 5eU-seq experiments were performed on MCF10A cells.
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bioRxiv - Cell Biology 2023Quote: ... siARP2/3 (5’- GGAUUCCAUUGUGCAUCAAtt-3’, 5’-GGGAUGAUGAGACCAUGUAtt-3’, 5’- AAAUCCUAAUGGAGACAAAtt-3’, Ambion)
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bioRxiv - Molecular Biology 2024Quote: ... overnight and then loaded with Fluo-4 (1:1000; Fluo-4 Calcium Imaging Kit; Invitrogen) and CellTracker Red (1:2000 ...
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bioRxiv - Bioengineering 2022Quote: Primary neonatal cardiomyocytes were isolated from C57BL/6 mouse neonates on postnatal days 3-5 using the Pierce Primary Cardiomyocyte Isolation Kit (Thermo Fisher) as previously described (14) ...
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bioRxiv - Cell Biology 2022Quote: ... Embedded tissues were cut into 3-6 µm sections using a paraffin microtome (Leitz) and placed on SuperFrost® Plus slides (Menzel Gläser, Thermo Scientific). Deparaffination and antigen retrieval was done as described previously (Link et al. ...
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bioRxiv - Neuroscience 2020Quote: ... All 27 samples from 3 groups and 6 GIS were divided and labeled using two sets of 5 mg 11 plex TMT reagents (Thermo Scientific A34808 ...
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bioRxiv - Immunology 2022Quote: ... and the wells were washed five times with wash buffer before the addition of 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS, #37615, Thermo Fisher Scientific). Optical density was read 40 min later at 405 nm using a plate reader (SpectraMax i3 ...
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bioRxiv - Cell Biology 2021Quote: ... The same amount of protein lysate was aliquoted into different PCR tubes and simultaneously subjected to 6 different temperatures (37, 41, 45, 49, 53, 57 °C) for 3 min in the Veriti Thermal Cycler (Applied Biosystems). After 3 min cooling on ice ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were expanded to 6 × 106/mL the day before transfection and were then diluted to 3 × 106/mL by ExpiCHO™ expression medium (Gibco). Plasmids were diluted to 20 μg/mL by cold OptiPRO medium (Gibco ...
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bioRxiv - Evolutionary Biology 2022Quote: ... was added to cDNA and the DNA was denatured at 95°C for 3 min prior to loading in Novex 6% TBE-urea gel (Thermo Fisher). Samples were separated for 45 min at 180 V ...
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bioRxiv - Neuroscience 2022Quote: ... 3.5 cm) containing 30 larvae and a plastic-covered stirrer (magnetic stir bar micro PTFE 6 mm x 3 mm; Fisher Scientific) were placed on a magnetic stir plate (Variomag Poly 15 stirrer plate ...
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bioRxiv - Neuroscience 2024Quote: Determination of sIL-6R was performed in the FN of NL and 21 dpi of aged WT and GFAP-IL6Tg (n=3-6/experimental group) using a precoated mouse ELISA kit (EMIL6RA, Fisher Scientific) and following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and TNFα (10 ng/ml) for 6 h and stained for cleaved Caspase 3/7 green (5 µM) and propidium iodide (2 µM) (Thermo Scientific) for an additional 30 min ...
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bioRxiv - Biochemistry 2023Quote: 1.3 million HEK293T cells were seeded in a 6 cm dish in 5 ml of DMEM [high glucose DMEM (Gibco, 12800), 3.7 g/L NaHCO3 ...
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bioRxiv - Neuroscience 2023Quote: HEK-293T cells were seeded on 6-well culture plates at 3 x 106 cells/well and grown in Dulbecco modified Eagle medium (DMEM; Thermo Fisher Scientific ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... NM_002524.5) with a 3’ terminal 6 × His-tag was chemically synthesized and cloned into the pFastBac1 vector (Invitrogen, Carlsbad, California, USA). cDNA of NRASQ61R was cloned into the pLVSIN-EF1α-AcGFP-C1 vector (Takara Bio Inc. ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 µL of primers (final concentration 200 nM) and 6 µL of Power SYBR Green PCR Master Mix (Thermo Fisher Scientific). The amplification conditions included an initial incubation for 2 min at 50°C ...
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bioRxiv - Genomics 2024Quote: ... Resulting mRNA was split into 6 separate reactions and cDNA was synthesized using a gene-specific primer (5’-CTCATCAATGTATCTTATCATGTCTG-3’) and MultiScribe Reverse Transcriptase (Invitrogen, 4311235). Following cDNA synthesis ...
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bioRxiv - Neuroscience 2024Quote: ... differentiated OLs at 6 DIV (‘OL 3 days’) were transfected with plasmids using Lipofectamine LTX with Plus Reagent (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2024Quote: ... were plated onto 6-well culture plates at 2×105 cells/well (concentration determined using the Countess 3 automated cell counter; Invitrogen: AMQAX2000) for downstream experiments (n=5 biological replicates/treatment) ...
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bioRxiv - Bioengineering 2024Quote: ... or sonication (SN) method (20% power with 3 seconds on/6 seconds off for 10 cycles using a Qsonica Sonicator Q700, Fisher Scientific). The incubated caveospheres (IN ...
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bioRxiv - Molecular Biology 2022Quote: ... pDNOR207-ZNF451-1 (isoform 1) and pDNOR207-ZNF451-3 (isoform 3) were generated using the Gateway® cloning BP reaction (Thermo Fisher Scientific) upon cDNA amplification using BP-tailed primers and pDNOR207 as donor vector ...
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bioRxiv - Neuroscience 2022Quote: ... the medium was replaced every 2-3 days and cells passaged 1:2 or 1:3 weekly with 0.25% Trypsin/EDTA (Thermo Fisher Scientific, #25200-056) pre-warmed at 37°C.