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Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for TIM 1 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and into pcDNA™6/myc-His A (Invitrogen), from an amplicon produced by RT-PCR from S2Xpress RNA ...
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bioRxiv - Biochemistry 2023Quote: ... His-Tag Dynabeads (Invitrogen 10103D, 10 µL per sample) was washed three times with B buffer before protein immobilization ...
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bioRxiv - Biophysics 2023Quote: His-Tag Dynabeads (Invitrogen 10103D, 10 μL per sample) were manually washed three times with B buffer and resuspended in B buffer (100 μL per sample) ...
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bioRxiv - Microbiology 2023Quote: ... araC and the araBAD promoter from pBAD/His (Invitrogen), the tetracycline resistance gene from pACE2 (Geneva Biotech ...
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bioRxiv - Plant Biology 2023Quote: ... Dynabeads His-Tag Isolation and Pulldown (Thermo Fisher Scientific) were washed twice in Strep-to-His Buffer before adding the eluted proteins and incubating while rotating for 20 min ...
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bioRxiv - Biochemistry 2024Quote: Supernatant was supplemented with His-Pur cobalt resin (ThermoFisher), imidazole to 10 mM ...
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bioRxiv - Cancer Biology 2021Quote: ... were established by using the Flp-In™ T-REx™-293 system (Invitrogen) (Figure S2) ...
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bioRxiv - Microbiology 2020Quote: Plasmids encoding S variants were transiently transfected into FreeStyle 293-F cells (Thermo Fisher) using polyethyleneimine ...
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bioRxiv - Immunology 2021Quote: ... by transfection with purified plasmid using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Supernatants from transfected cells were collected 3 days after transfection ...
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bioRxiv - Molecular Biology 2021Quote: Flp-In™ T-REx™ 293 Cell Line (parental line; R78007, ThermoFisher UK) were cultured in D-MEM™ (high glucose ...
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bioRxiv - Molecular Biology 2021Quote: ... Stable integration of CLIP-UPF1SL into human Flp-InTM T-RExTM-293 cells (Invitrogen) and subsequent maintenance of this stable line was previously described (Kishor et al ...
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bioRxiv - Molecular Biology 2020Quote: The 293 T cells were plated into Nunc 4-well chamber slides (ThermoFisher 154453) and transfected with indicated plasmids and siRNA ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were maintained in a 50:50 mixture of 293 SFM II (Gibco, 11686029) and CD CHO medium (Gibco ...
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bioRxiv - Immunology 2019Quote: ... HEK293F cells were maintained in FreeStyle 293 Expression Medium (Thermo Fisher Scientific, catalog 12338018) at 37 °C/8% CO2 ...
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bioRxiv - Immunology 2019Quote: ... 293 and 2934×4 knock-out clones were generated by transfection (lipofectamine 2000, Invitrogen) of CRISPR–Cas9-expressing knockout plasmids (MAVS ...
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bioRxiv - Immunology 2019Quote: ... 293F suspension cells were grown in Freestyle 293 expression medium (Life Technologies 12338-018) on a shaker incubator ...
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bioRxiv - Biochemistry 2019Quote: The Ca2+-flux assay was performed using Flip-In 293 cell line (Life Technologies) stably expressing full-length T1r2a ...
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bioRxiv - Microbiology 2021Quote: ... HEK293F cells were thawed and incubated with FreeStyleTM 293 Expression Medium (Life Technologies, CA) in a shaker incubator at 37 °C at 135 rpm with 8% CO2 ...
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bioRxiv - Biophysics 2021Quote: ... The recombinant protein was expressed in Expi 293 GnTI- (Thermo Fisher, Waltham, MA, USA) at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... using ExpiFectamine™ 293 Transfection Kit according to manufacturer’s directions (Thermo Fisher Scientific, USA). The cells were then cultured for 4-5 days and removed by centrifugation at 800×g for 5 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... using polyethylenimine in Freestyle 293F cells cultured in 293 FreeStyle media (Thermo Fisher Scientific). Where indicated ...
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bioRxiv - Microbiology 2022Quote: ... then re-suspended in 30 mls FreeStyle 293 Expression Medium (no. 12338018; Thermo Fisher). An aliquot of cells was taken for counting and the cells were spun again at 300 xg for 5 minutes ...
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bioRxiv - Bioengineering 2022Quote: Expi293F cells were transfected with the purified plasmids using ExpiFectamine 293 transfection kit (Invitrogen), where the fusion proteins were expressed and harvested at 96 hrs post-transfection under 8% CO2 at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... the medium was replaced with 30 ml FreeStyle 293 Expression Medium (Thermo Fisher Scientific). After 3 days ...
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bioRxiv - Molecular Biology 2019Quote: ... pMDG2 in 293-LX packaging cell line using lipofectamine LTX (15338500, Thermo Fisher Scientific). Viral supernatants were harvested 48 h post transfection and concentrated using Amicon Ultra-15 centrifugal filter units (UFC910024 ...
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bioRxiv - Immunology 2019Quote: ... Human and rabbit Abs were transiently expressed with the FreeStyle 293 Expression System (Invitrogen). Abs were purified using affinity chromatography (Protein A Sepharose Fast Flow ...
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bioRxiv - Biochemistry 2019Quote: ... were maintained in suspension in protein-free chemically-defined FreeStyle 293 Expression Medium (Invitrogen / Thermo Fisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... Human and rabbit Abs were transiently expressed with the FreeStyle 293 Expression System (Invitrogen). Abs were purified using affinity chromatography (Protein A Sepharose Fast Flow ...
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bioRxiv - Neuroscience 2021Quote: A stable cell line was generated from Flp-In T-Rex 293 cells (Invitrogen), according to the manufacturer recommendations ...
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bioRxiv - Biochemistry 2020Quote: ... were transiently transfected with the vectors using the ExpiFectamine 293 Transfection kit (Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2019Quote: Stable cell lines were produced using Flp-In™-293 cells (Thermo Fisher Scientific), authenticated by STR profiling (FTA barcode ...
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bioRxiv - Molecular Biology 2021Quote: Flp-In T-Rex 293 (human, female, embryonic kidney, epithelial; Thermo Fisher Scientific, RRID:CVCL_U427) cells were cultured in high-glucose ...
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bioRxiv - Immunology 2020Quote: ... Expi293F cells were cultured using 66% FreeStyle 293 Expression / 33% Expi293 Expression medium (ThermoFisher) and grown in polycarbonate baffled shaking flasks at 37 °C and 8% CO2 while shaking at 120 rpm ...
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bioRxiv - Immunology 2022Quote: Recombinant proteins were produced using the Freestyle™ 293 Expression System (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were transfected using TransIT®-293 Transfection Reagent (Mirusbio) and Opti-MEM (Thermofisher). After 6 to 12 hours post-transfection ...
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bioRxiv - Cell Biology 2022Quote: ... were grown in suspension culture in FreeStyle™ 293 Expression Medium (Thermo Fisher Scientific) to density of 4 x 106 cells/ml and harvested by centrifugation (500 x g ...
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bioRxiv - Synthetic Biology 2023Quote: ... culture media was replaced by Freestyle 293 Expression Medium (Fisher Scientific 12-338-026), supplemented with 50UI/mL penicillin/streptomycin (Corning 30001CI) ...
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bioRxiv - Microbiology 2023Quote: ... were transfected using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific, Waltham, MA, USA). Recombinant huBPI ...
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bioRxiv - Immunology 2023Quote: ... Monoclonal antibodies were produced using standard methods in FreeStyle™ 293-F Cells (Invitrogen) (91 ...
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bioRxiv - Microbiology 2023Quote: ... were transfected with expression constructs using the Expifectamine™ 293 Transfection Kit (Thermo Fisher) following the manufacturer’s guideline ...
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bioRxiv - Biophysics 2023Quote: ... HEK293F cells were cultured in 30 ml of Freestyle 293 serum-free medium (Gibco). For transfection of HEK293F cells ...
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bioRxiv - Immunology 2023Quote: ... FreeStyle™ HEK293-F cells were cultured in Freestyle™ 293 expression medium (Gibco).
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bioRxiv - Systems Biology 2023Quote: ... were used to generate tetracycline-inducible stable Flp-InTM-293 T-REx cells (Invitrogen). A 6-well plate was co-transfected with 0.5 µg of the pEXPR plasmid and 1 µg of the pOG44 Flp-recombinase expression vector (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... Flp-In™ T-REx™ 293 Cell Lines (Thermo Fisher, catalog number: R78007) expressing Snrpb endogenously from a single locus were generated according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... HEK293F cells were thawed and incubated with FreeStyleTM 293 Expression Medium (Life Technologies, CA) in a shaker incubator at 37°C at 135 rpm with 8% CO2 ...
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bioRxiv - Immunology 2023Quote: ... CE0-3 SOSIPs were expressed in FreestyleTM 293-F cells (ThermoFisher Cat No. R79007). The modified CH505 transmitted founder Env SOSIPs were chimeras containing the CH505 gp120 and BG505 gp41 domains ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti- HO-1-1 (Thermo Fisher Scientific, MA1-112, 1:1000); rabbit anti-GAPDH (GeneTex ...
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bioRxiv - Molecular Biology 2020Quote: ... and ZO-1 (1:200 mouse anti-ZO-1, Invitrogen, 61-7300) with species-appropriate secondary antibodies (1:400 Invitrogen anti-chicken/-rabbit/-mouse Alexa 488/546/647 ...
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bioRxiv - Microbiology 2024Quote: ... C-His-OipA protein was purified by using Dynabeads™ His-Tag Isolation and Pulldown magnetic bead system (Thermo Fisher Scientific, United States). His-tagged OipA protein isolation procedure was carried out according to kit instructions ...
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bioRxiv - Bioengineering 2022Quote: ... Mouse anti-ZO-1 (#33-9100; Life Technologies; 1:125), Rabbit anti-ECAD (#3195S ...