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Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for TIM 1 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... 20 μg of the plasmid was transfected into 20 mL of Expi293F cells at 3 × 106 cells mL-1 using ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: His-tagged soluble human ACE2 was produced in FreeStyle™ 293-F cells by transfecting soluble human ACE2 (1-741 aa) expression vector plasmid DNA using 293fectin (Thermo Fisher) and purified using HiTrap Chelating column charged with nickel (GE healthcare ...
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bioRxiv - Microbiology 2021Quote: ... The plasmids were transiently co-transfected into Expi293F cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ 293 Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... was expressed in Expi293 cell line (3X106 cells/ml, 200 ml in 1 L flask) using ExpiFectamine™ 293 Transfection Kit (Gibco, A14524). After 48 hours ...
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bioRxiv - Biochemistry 2024Quote: ... Purified pOG44 and pcDNA5/FRT-SUGCT plasmids were co-transfected into Flp-In 293 cells at a 9:1 ratio by using Lipofectamine 2000 reagent (Invitrogen, Thermo Fisher Scientific). Flp-In 293 cells were cultured in DMEM supplemented with 10% FBS ...
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bioRxiv - Bioengineering 2024Quote: A mixture of 1 μg of antibody-expressing plasmid was transfected into FreeStyle 293 cells using the 293fectin Transfection Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions and cultured in FreeStyle 293 Expression Medium (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... They were then rinsed in block for >30 min at room temperature before incubation in secondary antibody (1:250 Invitrogen #A21-42 AF488 anti-mouse IgM; 1:250 Life Technologies #A11029 AF488 anti-mouse IgG1; 1:250 Invitrogen #A21141 AF488 anti-mouse IgG2b) in blocking solution for > 1 hour at room temperature or overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... and secondary antibody (anti-mouse IgG2a AF647, 1:2000, Invitrogen, A-21241; anti-mouse IgG AF568, 1:2000, Invitrogen, A-11031; anti-mouse Cy5, 1:1000), sequentially ...
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bioRxiv - Biochemistry 2019Quote: ... SAMS peptide substrate (His-Met-Arg-Ser-Ala-Met-Ser-Gly-Leu-His-Leu-Val-Lys-Arg-Arg, obtained from Invitrogen, USA) was used for the assay ...
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bioRxiv - Biochemistry 2020Quote: ... the magnetic beads were resuspended in 10.0625 μL ROX/Hi-Di (0.0625 μL of ROX 350 ladder, Applied Biosystems, in 10 μL of Hi-Di formamide, Applied Biosystems) and incubated at room temperature for 20 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... the magnetic beads were resuspended in 10.0625 μL ROX/Hi-Di (0.0625 μL of ROX 350 ladder [Applied Biosystems] in 10 μL of Hi-Di formamide [Applied Biosystems]) and incubated at room temperature for 20 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Cry1 pcDNA4A-myc-his and Cry2 pcDNA4A-myc-his plasmids were double digested with NotI and XhoI FastDigest enzymes (Thermo Scientific) and used as host plasmids ...
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bioRxiv - Biochemistry 2023Quote: Plasmids of pET28a-his TEV-glnR or pET28a-his TEV-glnR derivative was firstly transformed into expression strain BL21(DE3) (Invitrogen, Inc.). then single colonies of the positive transformants were inoculated and amplified with 5 L LB broth supplemented with 50 μg/ml kanamycin at 37 °C with shaking ...
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bioRxiv - Neuroscience 2023Quote: ... TMEM119 (mouse primary, Biolegend, 853302, 1:250, donkey anti-mouse 488 Life Technologies, A21202, 1:1000), and CX3CR1 (PE-rat ...
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bioRxiv - Microbiology 2020Quote: Plasmids encoding spike variants were transfected into FreeStyle 293-F cells (Thermo Fisher) in 3 mL of medium and harvested four days after transfection ...
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bioRxiv - Cell Biology 2021Quote: Flp-In™ T-Rex™ 293 cells were cultured in DMEM (Gibco) supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2021Quote: Suspension-adapted HEK293 cells (Freestyle™ 293-F cells, R790-07, Life Technologies) were grown as recommended in Freestyle™ 293 Expression Medium (12338026 ...
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bioRxiv - Molecular Biology 2021Quote: ... were grown as recommended in Freestyle™ 293 Expression Medium (12338026, Life Technologies,) sharking at 37°C in 5% CO2 ...
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bioRxiv - Biophysics 2021Quote: ... Suspension cultures of HEK293S GnTI− cells were grown in Freestyle 293 media (GIBCO) supplemented with 2% FBS (GIBCO ...
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bioRxiv - Biochemistry 2022Quote: ... Human FLP-In™ T-REx™ 293 Cell Lines (Invitrogen™, R78007) expressing hnRNPA1 wild type or D262V mutant cDNAs were generated by cotransfecting the hnRNPA1 cDNAs with a FLP recombinase expression plasmid according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmids were transiently transfected using the ExpiFectamine 293 Transfection Kit and protocol (GIBCO).
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bioRxiv - Immunology 2022Quote: The generated plasmids were transfected into FreeStyle 293-F cells (Thermo Fisher Scientific) using the FreeStyle MAX reagent ...
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bioRxiv - Immunology 2020Quote: ... Transfection of Expi293 cells was carried out by ExpiFectamine 293 Transfection Kit (ThermoFisher) according to the manufacturer instruction ...
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bioRxiv - Microbiology 2020Quote: ... were cultured in serum-free medium (Freestyle™293 Expression Medium, Invitrogen, USA) at 37°C with 5% of CO2 in an orbital shaker at 120 rpm ...
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bioRxiv - Biochemistry 2020Quote: ... Flp-In T-REx 293 cells were cultured in DMEM (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2020Quote: ... were transfected with packaging vectors in 293 cells using Lipofectamine 3000 reagent (Thermofisher), and viral supernatant was isolated and used to infect GBM cells ...
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bioRxiv - Immunology 2020Quote: ... The protein was expressed in FreeStyle 293-F cells (ThermoFisher Scientific; Cat# R79007) and purified by immuno-affinity purification using 2G12 mAb as previously described 7 ...
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bioRxiv - Molecular Biology 2022Quote: ... were established by using the Flp-In™ TREx™-293 system (Invitrogen)(77) ...
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bioRxiv - Immunology 2022Quote: ... grown in 125 ml flasks in FreeStyleTM 293 Expression Medium (ThermoFisher Scientific 12338018) were transfected at cell densities between 8.0×105 to 1.0×106 cells/ml with equal masses of plasmids containing the heavy chain and light chain genes using FreeStyleTM MAX Reagent (ThermoFisher Scientific 16447100) ...
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bioRxiv - Immunology 2022Quote: ... in the presence of 2mL of FreeStyle 293 Expression Medium (12338018, Gibco, USA) supplemented with 1% Antibiotic–Antimycotic (Sigma ...
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bioRxiv - Biochemistry 2019Quote: Expi293F cells: Transfections were performed using the ExpiFectamine™ 293 Transfection Kit (Gibco) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: ... and 1x forskolin solutions were made in CD 293 media (Thermo Fisher Scientific) supplemented with 4 mM L-Glutamine (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2021Quote: ... 125 rpm with 8% CO2 in FreeStyle™ 293 expression medium (Gibco, 12338018) to a cell density of 106 cells/ml ...
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bioRxiv - Synthetic Biology 2020Quote: ... Flp-In™ T-REx™ 293 cells were purchased from Invitrogen (ThermoFisher) and transfected with both the Flp recombinase vector (pOG44 ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293S GnTi- cells were cultured in Freestyle 293 expression medium (Thermo Fisher Scientific) at 37 °C with 5% CO2 ...
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bioRxiv - Biochemistry 2021Quote: ... The HEK293S-GnTi− cells were cultured in Freestyle 293 medium (Thermo Fisher Scientific) supplemented with 5% CO2 at 37 ° C ...
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bioRxiv - Immunology 2021Quote: ... and clone 2 hole light chain plasmid) with ExpiFectamine 293 transfection kit (Thermofisher). The expression and antibody purification protocol was similar to recombinant antibody expression described as above ...
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bioRxiv - Cell Biology 2019Quote: ... HEK293T and Flp-In T-REx 293 cells were cultured in DMEM (Gibco) supplemented with 10% heat-inactivated fetal bovine serum (Peak ...
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bioRxiv - Cell Biology 2020Quote: ... and transfected into human Flp-In T-REx 293 cells (Invitrogen, Carlsbad, CA). Following hygromycin selection and cell expansion ...
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bioRxiv - Microbiology 2020Quote: ... We maintained HEK293T cells grown in suspension in FreeStyle 293 Expression Medium (Gibco) and HEK293S GnTI-/- cells (ATCC CRL-3022 ...
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bioRxiv - Microbiology 2020Quote: ... cell line was cultured in a Freestyle 293 expression media (ThermoFisher Scientific, #12338026). For establishing the HEK293T/hACE2 stable cell line ...
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bioRxiv - Microbiology 2021Quote: ... 293F cells were cultured in Freestyle 293 expression medium (Invitrogen, Carlsbad, CA, USA). GPCLASV (Josiah strain ...
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bioRxiv - Immunology 2022Quote: ... These cell lines were incubated in Freestyle 293 expression medium (Thermo Fisher Scientific) at 37°C for 10-15 days ...
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bioRxiv - Immunology 2022Quote: ... HEK293F suspension cells were cultured in 293 Freestyle media (Gibco BRL Life Technologies) and cultured in a shaking incubator at 37°C ...
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bioRxiv - Immunology 2022Quote: ... HEK293F suspension cells were cultured in 293 Freestyle media (Gibco BRL Life Technologies) and cultured in a shaking incubator at 37°C ...
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bioRxiv - Microbiology 2022Quote: A stable Flp-In™ T-REx™ 293 cell line (Thermo Fisher) expressing the prefusion-stabilized HexaPro variant of the Wu Spike 68 was generated according to the manufacturer’s protocol (polyclonal selection) ...
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bioRxiv - Immunology 2022Quote: ... HEK293F suspension cells were cultured in 293 Freestyle media (Gibco BRL Life Technologies) and cultured in a shaking incubator at 37°C ...
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bioRxiv - Immunology 2022Quote: ... HEK293F suspension cells were cultured in 293 Freestyle media (Gibco BRL Life Technologies) and cultured in a shaking incubator at 37°C ...
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bioRxiv - Biochemistry 2023Quote: HEK293S GnTI− cells were grown at 37°C in Freestyle 293 medium(Invitrogen) supplemented with 1.5% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... the HEK293-derived cells Flp-InTM T-RexTM 293 Cell Line (ThermoFisher Scientific) were stably transfected to express HA-TDP-43 upon induction with tetracycline (1 μg/mL) ...