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Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for Recombinant Mouse Folh1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... The pIgR gene was inserted into the pEF-Myc-His vector (ThermoFisher Scientific, MS, USA).
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bioRxiv - Developmental Biology 2023Quote: ... We also subcloned this cassette into a pAc5.1/V5-His A plasmid (Invitrogen #V4110-20) by cutting the cassette out of pUASP-attb-Cry2-CTEV-mCherry using NotI and XbaI (NEB #R3189S and #R0145S ...
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bioRxiv - Immunology 2023Quote: ... and cryopreserved in heat-inactivated fetal-bovine serum (HI-FBS, ThermoFisher Gibco™ #12484028, USA) + 10% (v/v ...
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bioRxiv - Immunology 2023Quote: ... and cryopreserved in heat-inactivated fetal-bovine serum (HI-FBS, ThermoFisher Gibco™ #12484028, USA) + 10% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... which were pre-loaded with 10 μL of Hi-Di™ formamide (ThermoFisher, Waltham, USA) and GeneScan™ -400HD ROX™ Size standard (ThermoFisher ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The PCR product was mixed with Hi-DiTM mix (Thermo Fisher Applied BiosystemsTM, Carlsbad, USA) with GeneScantm LIZ dye Size standardtm (Thermo Fisher Applied BiosystemsTM ...
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bioRxiv - Molecular Biology 2024Quote: ... in HepG2 medium (Advanced MEM minus L-Glutamine [Gibco] + 10% [v/v] HI-FBS [Gibco] + 1% [v/v] penicillin-streptomycin + 2 mM Glutamax [Gibco] and 0.1% [v/v] amphotericin B) ...
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bioRxiv - Microbiology 2024Quote: ... and passed through a 3 mL His-Pur Ni-NTA column (Thermo Scientific, catalog # 88226) according to manufacturer instruction ...
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bioRxiv - Immunology 2024Quote: ... Blot was then incubated with 1:1000 dilution anti-his conjugated to HRP (Thermo Scientific) in 5% milk in 1xTBST (1 h ...
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bioRxiv - Immunology 2024Quote: ... Amplified PCR fragments were inserted into the metallothionein promoter driven pMT-V5-His vector (Invitrogen). The generated vector was co-transfected with a pCoBlast blasticidin resistance vector with a ratio of 19:1 and cells were selected in blasticidin containing medium according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... mouse anti-mouse PCSK9 (Invitrogen), mouse anti-mouse vinculin (Santa Cruz) ...
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bioRxiv - Plant Biology 2020Quote: ... To detect V5-tagged proteins a 1: 5,000 dilution of the primary anti-V5 antibody (Invitrogen, #R96025) and an anti-mouse horseradish peroxidase-conjugated secondary antibody (1:10,000 dilution ...
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bioRxiv - Biophysics 2021Quote: ... The GFP-tagged and the knockout strains come from the commercial Yeast GFP clone collection (ThermoFisher Scientific). All cultures were grown in YPD medium (1% Yeast Extract ...
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bioRxiv - Genetics 2021Quote: ... The ORF was Gateway cloned into a C-terminal GFP-tagged Gateway pcDNA-DEST47 vector (ThermoFisher Scientific), sequence verified ...
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bioRxiv - Molecular Biology 2021Quote: ... HA-tagged hamster SCAP was prepared by subcloning PCR products into the pcDNA5/FRT/TO vector (Invitrogen). DsRed-ER plasmids were purchased from Addgene (#55836) ...
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bioRxiv - Molecular Biology 2022Quote: GST-tagged bait proteins were purified using Pierce GST Protein Interaction Pull-Down kit (Thermo-Fisher Scientific). To examine recombinant Pin1 phosphorylation ...
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bioRxiv - Molecular Biology 2021Quote: ... and Myc-tagged human Insig1 (NM_005542) were prepared by subcloning PCR products into the pcDNA3 vector (Invitrogen). YFP-tagged human RHBDL4 and YFP-human SREBP-1c-CFP were prepared by subcloning PCR products into the pCI-EYFP vector ...
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bioRxiv - Biochemistry 2019Quote: ... 5 μg (otherwise noted) of terminally tagged and unmodified σ1R plasmid was transfected using lipofectamine 2000 (Invitrogen) for HEK 293T Δσ1R cells in a 10 cm plate ...
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bioRxiv - Neuroscience 2019Quote: ... GFP-Msp300KASH tagged OSN nuclei were pulled down using a Chicken anti-GFP antibody (Invitrogen #PA1-9533) bound to magnetic Dynabeads™ Protein G (Invitrogen #10003D) ...
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bioRxiv - Microbiology 2019Quote: ... coli MG1655 cells expressing tagged HupA-GFP with 2 µg/ml of FM4-64 dye (Invitrogen, USA) and incubating for 5 min at 37°C with shaking at 180 rpm ...
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bioRxiv - Cancer Biology 2019Quote: ... EdU was tagged with Alexa Fluor 647 picolyl azide through click reaction (Click-iT chemistry, ThermoFisher, C10640). The cells were blocked with blocking buffer at least overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Pierce anti-HA beads for immunoprecipitation of HA-tagged GAPDH was purchased from Thermo Fisher (Cat. # 88836) Rabbit polyclonal antibody (Cat ...
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bioRxiv - Neuroscience 2021Quote: ... Epitope-tagged proteins were eluted from beads by boiling for 5 min in LDS sample buffer (ThermoFisher). Anti-D-cysteine immunoprecipitates were eluted by incubating beads in 1 mM D-cys in TBS for 30 min at room temperature ...
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bioRxiv - Physiology 2021Quote: ... incubated with different primary Abs and revealed by appropriate Alexa-Fluor-tagged secondary Abs (Thermo Fisher Scientific). Cells were analyzed by using a Nikon Ti Eclipse inverted microscope with A1 scanning confocal microscope at the Confocal and Specialized Microscopy Shared Resource (CSMSR) ...
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bioRxiv - Cell Biology 2021Quote: ... incubated with different primary Abs and revealed by appropriate Alexa-Fluor-tagged secondary Abs (Thermo Fisher Scientific). Slides were mounted in Fluoromount-G (Southernbiotech ...
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bioRxiv - Neuroscience 2020Quote: ... The fluorescent-tagged secondary antibodies Alexa Fluor 488 and Alexa Fluor 555 (1:500, Thermo Fisher Scientific) were used for detection ...
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bioRxiv - Cell Biology 2021Quote: cDNAs in pENTR or pDONR were transferred into tagged mammalian expression vectors using Gateway® recombination (Invitrogen): pCAG-DEST-EGFP ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were transfected with 200 ng of Flag-tagged FOXQ1 using Lipofectamine 2000 (Thermo Fisher, Waltham, USA). After 24 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... Species-specific secondary antibodies tagged with Alexa Fluor corresponding to 568 nm emission spectra (1:1,000, Thermofisher) was used for immunofluorescence ...
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bioRxiv - Microbiology 2022Quote: ... HNRNPUL1 and MECR were recombined into V5-tagged mammalian expression constructs by Gateway cloning into pcDNA6.2 (Invitrogen) and pLX304 (Addgene 25890) ...
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bioRxiv - Immunology 2019Quote: ... and counter-stained with fluorophore-tagged goat anti-rabbit or anti-human IgG (H+L) (Life Technologies) respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... Flag tagged proteins were immunoprecipitated from 2-3mg cellular extracts with Pierce Anti-DYKDDDDK Magnetic Agarose (Invitrogen). Beads were washed 5x with 1ml IP lysis buffer end eluted with 50µl 1xLaemmli buffer ...
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bioRxiv - Biochemistry 2020Quote: ... 1 μg of Flag-Strep-Strep-(FSS)-tagged LRRK2RCKW cDNA and Lipofectamine 2000 reagent (ThermoFisher Scientific, USA) were used according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... while non-Avi tagged antigens were biotinylated chemically using EZ-Link Sulfo-NHS-Biotin (Thermo Fisher, A39256). VH and VL sequences of SARS-CoV-2 control antibodies (COV2-2196 ...
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bioRxiv - Cell Biology 2021Quote: ... Cross-absorbed secondary antibodies tagged with Alexa-fluorophores were diluted in blocking buffer (1:400, Invitrogen, Switzerland) and applied for 30 min at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK293 and MS2-tagged HEK293:24xMS2-NEAT1 cells were cultured in Dulbecco’s modified eagle’s medium (DMEM, Gibco) with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... They were then transfected with Halo-tagged gene constructs using Lipofectamine LTX (Thermo Fisher Scientific, MA, USA). After 48 hours ...
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bioRxiv - Immunology 2024Quote: ... FLAG-tagged constructs were PCR amplified with Gateway adapters and cloned into pDONR221 using BP Clonase (Invitrogen). Hoil1 and Hoip mutations and deletions were generated using the Q5 site-directed mutagenesis kit (New England Biolabs) ...
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bioRxiv - Cell Biology 2024Quote: ... TwinStrep-tagged proteins were concentrated using 30K MWCO concentrators to ∼ 5 mg/ml (Thermo Scientific™, USA). Protein was stored at −80°C.
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bioRxiv - Developmental Biology 2024Quote: ... inserts were then subcloned into pCS2+ N HA tagged vectors that had been converted into Gateway (Invitrogen) destination vectors ...
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bioRxiv - Cell Biology 2024Quote: HEK cells and zebrafish samples were tagged with TMT10plex™ Isobaric Label Reagent Set (Thermo Scientific, #90111), and fibroblast cells with TMTpro™16plex Isobaric Labels (Thermo Scientific ...
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bioRxiv - Biophysics 2023Quote: ... they were transfected with the S6-tagged human TrkA by means of Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein A-tagged strains were immunoprecipitated with Rabbit IgG-conjugated (MP-Biomedicals, SKU 085594) Dynabeads (Invitrogen, 14301) (Vakiloroayaei et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... IPs of FLAG-tagged PRRC2B and PRRC2B fragments were performed using anti-DYKDDDDK Magnetic agarose (ThermoFisher Scientific). Beads were mixed with 70 μl of SDS loading buffer (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubation with fluorescence-tagged secondary antibodies (Molecular Probes Alexa series, all 1:400 in PBS) for 1h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... VF-tagged human SYK and FCER1G plasmids for mammalian expression were generated using Gateway cloning technology (Invitrogen) as described previously (47) ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were incubated with Alexa Fluor 555 tagged donkey-α-rabbit secondary antibody (1:1000; Molecular Probes) and Alexa Fluor 488-tagged donkey-α-goat secondary antibody (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... Biotin-tagged DNA nicks were visualized using Alexa488-or Alexa647-conjugated streptavidin (Molecular Probes, diluted 1/1000) during the incubation with the secondary antibody.
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bioRxiv - Immunology 2023Quote: ... The supernatant fraction containing the GST-tagged constructs were loaded onto Protino Glutathione Agarose 4B (Fisher Scientific) beads and impurities were removed by washing with TBS ...
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bioRxiv - Cell Biology 2023Quote: MDA-MB-231 clones H2 and C10 carrying endogenously tagged HiBiT-cIAP1 were generated by Thermo Fisher and were used for compound screens ...