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Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for Recombinant Mouse Erbb2 protein Fc tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... recombinant SARS-CoV-2 spike protein was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Pathology 2021Quote: ... His-tag recombinant SARS-CoV-2 Spike Protein S1/S2 (S-ECD) (1 ng/ml; ThermoFisher Scientific; aa11-1208; RP-87680) or Flag-tag recombinant SARS-CoV-2 Spike RBD (1 ng/ml ...
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bioRxiv - Immunology 2021Quote: Monoclonal antibodies or recombinant SARS-CoV-2 RBD + SBD1 or NTD proteins were transfected in FreeStyle 293F suspension cells (Life Technologies) using PEIMAX transfection reagent (Polysciences) ...
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bioRxiv - Immunology 2021Quote: Thermal melt analysis of the recombinant proteins was performed in triplicate in 96-well plates in a QuantStudio 7 Flex real time PCR machine (Applied Biosystems). Each well contained 2-4 μg protein in buffer (25mM TRIS pH 8 ...
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bioRxiv - Molecular Biology 2020Quote: ... # 236-EG), 20 ng/ml recombinant human FGF basic protein (R&D, # 4114-TC),1% penicillin/streptomycin (P/S, Invitrogen, SV30010), 1% sodium pyruvate (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... Cells were seeded in 96-well-plates and treated with 10μg/ml OM-85 concentrate (OM-Pharma) or 100 ng/mL of human recombinant IFN-β protein (ThermoFisher Scientific). The concentration of 10μg/ml of OM-85 was based on previous data with RSV we recently generated [9] ...
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bioRxiv - Molecular Biology 2022Quote: ... Then 3 μg of purified recombinant protein was added with 4 μl of RiboLock RNase Inhibitor (Thermo Fisher Scientific, Waltham, MA). The mixture was incubated at room temperature for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 Spike RBD 319-541 recombinant protein (OBA0101, Ovodan Biotech) was expressed in 25 mL culture using the Expi293F expression system (#A14635; ThermoFisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The cells were treated with the appropriate concentration of recombinant proteins and YO-PRO (Thermo Fisher Scientific, diluted at 1:2000) and incubated at 37°C ...
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bioRxiv - Biochemistry 2023Quote: Recombinant trimeric spike proteins (5 µg/mL) were plated in 50 µl in each well on a MaxiSorp (Thermo Fisher Scientific) microtiter plate in 1xPBS and left to incubate for at least 1 h at room temperature ...
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bioRxiv - Genetics 2023Quote: ... The presence of recombinant CENH3 proteins on the membrane was always verified by detection with the HisG epitope tag antibody (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... PIK3CA/PIK3R1 (p110a/p85a) recombinant human protein for use in the experiment shown in Figure 4F was purchased from Thermo Fisher Scientific (catalog number PV4788).
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bioRxiv - Immunology 2023Quote: ... designed using CRISPOR) and 7.5 μg recombinant Cas9 protein (Vienna Biocenter Core Facilities) using the Neon Transfection System (Thermo Fisher Scientific). A week later ...
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bioRxiv - Molecular Biology 2023Quote: About 3 ml of bleed 3 was diluted in 7 ml of sterile 1X PBS and passed through 0.2 μM filter and incubated with recombinant protein G sepharose 4B beads (Life Technologies 101242) at 4℃ overnight ...
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bioRxiv - Microbiology 2024Quote: ... Purity of the recombinant PfPKG proteins was assessed by SDS-PAGE using NuPAGETM Novex® 3-8 % Tris-Acetate gels (Invitrogen) and expression was confirmed by Western blot analysis.
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bioRxiv - Bioengineering 2024Quote: ... The cell clumps in Matrigel dome were cultured in the EM supplemented with 25 ng/mL human active BMP7 recombinant protein (Gibco; PHC7204) initially for 3 - 6 days to regenerate and form Exp-HLOs using BMP7 ...
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bioRxiv - Cell Biology 2022Quote: ... The Alexa Fluor 555 (AF555) labeled CCL19 was prepared with Alexa Fluor™ 555 Microscale Protein Labeling Kit (Invitrogen, A30007) according to manufacturers’ instructions ...
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bioRxiv - Biophysics 2021Quote: Scc2/4 was labeled by mixing the protein in an equimolar ratio with Qdot 705 Strep Conjugate (Thermo Fisher Scientific) and incubating for 10 min on ice in BSA buffer (40 mM Tris ...
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bioRxiv - Neuroscience 2021Quote: ... SP was labeled separately with Alexa Fluor 555 using a kit (Microscale protein labeling kit; ThermoFisher Scientific; Waltham, MA, USA) and by following the manufacturer instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... These two proteins were labeled at the cysteine using thiol-reactive BODIPY™ FL N-(2-Aminoethyl) Maleimide (Thermo Fisher). The proteins were mixed with 10-fold excess BODIPY™ FL N-(2-Aminoethyl ...
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bioRxiv - Neuroscience 2022Quote: ... The labeled DDX5-SNAP protein was subjected to desalting where the column (Zeba spin desalting columns, Thermo Fisher, Cat# 89882) was pre-equilibrated with high salt buffer (1 M NaCl ...
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bioRxiv - Physiology 2022Quote: O-GlcNAc modified proteins were labeled using Click-iT™ O-GlcNAc Enzymatic Labeling System (Thermo Fisher Scientific, Waltham, MA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Purified rLytA and rLytA* were labeled with the Alexa Fluor594 protein labeling kit according to manufacturer instructions (Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2022Quote: The αEGFR-Fn3-DHFR2-CVIA protein was non-specifically labeled with DyLight™ 650 NHS Ester (Thermo Scientific, Cat: 62266) in PBS according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... HEp-2 cells were transiently transfected with an expression vector of fluorescently labeled proteins using Lipofectamine LTX & Plus Reagent (Invitrogen) for 48 h before stress exposure ...
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bioRxiv - Cell Biology 2022Quote: ... were prepared as described above except that ConA-coated beads were incubated with biotinylated BSA previously labeled with an Alexa Fluor 555 protein labeling kit (Invitrogen). Unlabeled beads (FN-coated ...
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bioRxiv - Microbiology 2020Quote: Aliquots of 100 μg of the different SadP protein preparations were labeled with 125I by the Iodogen method according to the manufacturer’s instructions (Pierce/Thermo Scientific), giving approximately 2000 cpm/μg protein.
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bioRxiv - Neuroscience 2020Quote: ... Beads were washed four times with PBS containing protease inhibitors and protein were labeled with 2 mM Alexa 488 C5 maleimide or Alexa 546 C5 maleimide (Invitrogen) overnight at 4 °C in the dark ...
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bioRxiv - Biochemistry 2021Quote: ... we used the same amount of chromatin proteins for different conditions and labeled them with different amine-reactive TMT 6-plex reagents (ThermoFisher). Then we mixed these samples together and carried out mass spectrometry analysis ...
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bioRxiv - Immunology 2021Quote: ... The RBD probe was prepared at a molar ratio of 4 moles of protein to 1 mole of Alexa Fluor 647-labeled streptavidin (Invitrogen). PBMCs from the five participants were thawed and stained for SARS-CoV-2-specific IgG+ memory B cells ...
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bioRxiv - Microbiology 2020Quote: ... These two proteins were labeled at the cysteine using thiol-reactive BODIPY™ FL N-(2-Aminoethyl) Maleimide (Thermo Fisher). The proteins were mixed with 10-fold excess BODIPY™ FL N-(2-Aminoethyl ...
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bioRxiv - Microbiology 2022Quote: ... The membrane containing the biotin alkyne labeled proteins was incubated in a solution containing 1% BSA with streptavadin-HRP (Invitrogen) for subsequent visualization.
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bioRxiv - Immunology 2023Quote: ... 0.5 mls of Sm/RNPs (0.75 mg/mL) were labeled using the Alexa Fluor 647 Protein Labeling Kit (Invitrogen A20173) following the manufacturer’s instructions without removal of unbound AF647.
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bioRxiv - Immunology 2024Quote: ... 21330) and the resulting biotin-labeled protein was purified through a Zeba quick spin column (Thermo Scientific cat no. 89882). The RBD-biotin was prebound with streptavidin-AlexaFluor-647 to make RBD-647 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Zebrafish Kcnc3a channel was labeled using a polyclonal antibody originally generated against the human Kcnc3 protein (ThermoFisher Scientific PA5-53714) at a concentration of 2.5 μg/ml ...
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bioRxiv - Immunology 2020Quote: Recombinant SARS-CoV-2 S-RBD fused with Fc/His tag (S-RBD-His) was produced in 293T cells and purified with Protein A Agarose (Thermo Fisher Scientific). Recombinant human ACE2-ECD fused with Flag/His tag was produced in 293T cells and purified with anti-DYKDDDDK G1 Affinity Resin according to the manufacturer’s instructions (GenScript).
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bioRxiv - Developmental Biology 2019Quote: CIL in single cells encountering cadherin coated beads used cdh3-coated beads prepared by binding purified cdh3-fc (gifted from Dr. Douglas W. DeSimone and Dr. Barry. M. Gumbiner) to Protein A/G coated beads (53132, Thermo Fisher Scientific) (Chappuis-Flament et al. ...
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bioRxiv - Microbiology 2022Quote: ... 4a3A cells were maintained at 27°C without CO2 in Insect-XPRESS™ Protein-free Insect Cell Medium complemented with heat-inactivated Fetal Calf Serum (FCS) (#A3840002 ThermoFisher Scientific). C6/36 cell line (ATCC ...
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bioRxiv - Immunology 2024Quote: ... tagged with human IgG Fc was transiently transfected using FreeStyle 293-F cells and purified using Protein G Plus Agarose (Pierce Thermo Fisher Scientific). RBD-SD1 was cleaved from the Fc by incubation with 3C protease (produced in-house ...
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bioRxiv - Immunology 2022Quote: ... 100 ng/mL Human Recombinant IL-4 and 250 ng/mL Human Recombinant GM-CSF (ThermoFisher) for six days at 37°C and 5% CO2 ...
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bioRxiv - Biochemistry 2020Quote: ... Cytosolic extracts were generated as described above and the His6-tagged proteins purified by metal affinity chromatography and gravity flow using 0.5 ml HisPur™ cobalt superflow agarose (Thermo Fisher Scientific™, Waltham, USA) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... immunogen SARS-CoV-2 spike protein that interact with HeLa cell expressed SARS-CoV-2) and anti ACE2 antibody (ACE2 Recombinant Rabbit Monoclonal Antibody (Cat# MA532307; interact with mouse ACE2) were obtained from Life Technologies Corporation.
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bioRxiv - Immunology 2020Quote: ... CD8α+IEL were expanded in complete medium containing 50 ng/ml recombinant mouse IL-15/IL-15R complex (Thermo Fisher Scientific) on a tissue culture plate at the concentration of 5×105 cells/ml for 3 days ...
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bioRxiv - Genetics 2022Quote: ... Secondary antibodies: Goat anti-Mouse IgG (H+L) Superclonal Recombinant Secondary Antibody Alexa Fluor 555 (1:200, ThermoFisher Scientific catalogue #A28180), Goat Anti-Rabbit IgG H&L Alexa Fluor 488 (1:200 ...
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bioRxiv - Cell Biology 2019Quote: ... Alexa Fluor 594 labeled goat anti-mouse IgG IgG(H+L), F(ab’)2 Fragment (A-11020, (Molecular Probes, Inc.), Alexa Fluor 647 conjugate-Anti-Rabbit IgG(H+L) ...
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bioRxiv - Bioengineering 2020Quote: ... and/or specifically labeled using the immunofluorescent antibodies Mouse CD9 Monoclonal (MEM-61)-Alexa Fluor® 647 antibody (Thermo Fisher, Catalog# MA5-18154 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then rinsed in 1x PBS and labeled with Alexa Fluor 488 anti-mouse (ThermoFisher #A28175, Waltham, MA, USA) and Alexa Fluor 594 anti-rabbit (ThermoFisher #A-11012 ...
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bioRxiv - Cell Biology 2021Quote: ... Alkaline phosphatase (AP)-labeled goat anti-mouse IgG (H+L) cross-adsorbed secondary Ab (G-21060) was from Thermo Fisher Scientific.
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bioRxiv - Microbiology 2020Quote: ... After incubating with Alex647 labeled Rabbit anti-mouse IgG secondary antibody (Catalog No. A-21239, Thermo Fisher Scientific, Waltham, MA) and washing ...
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bioRxiv - Immunology 2022Quote: ... Deposition of immunoglobulin (IgG) and complement component 3 (C3) in the kidney tissue were stained with PE labeled goat anti-mouse IgG (Invitrogen) and FITC labeled rat anti-mouse C3 (Cedarlane ...