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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for Recombinant Mouse Erbb2 protein Fc tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: ... coli phosphate-binding protein labeled with 7-Diethylamino-3-[N-(2-maleimidoethyl)carbamoyl]coumarin (MDCC) (phosphate sensor, CAT # PV4406, Thermo Fisher) upon binding of the free phosphate GTP hydrolysis product (excitation at 425 nm ...
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bioRxiv - Evolutionary Biology 2021Quote: ... surface proteins were labeled with biotin by adding 1 mg/ml membrane impermeant EZ-LinkTM Sulfo-NHS-SS-Biotin (Thermo Fisher) in 137 mM NaCl and 15 mM sodium borate ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Cell surface proteins were labeled by incubating cells with 1.6 mM Sulfo-NHS-SS-Biotin (Thermo Fisher Scientific, Waltham, MA USA) or 1.6 mM Biotin-dPEG3-Maleimide (MilliporeSigma ...
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bioRxiv - Microbiology 2020Quote: ... Reference was prepared by mixing equal amounts (by total protein) of all samples and labeled with Alexa Fluor 555 (Thermo Fisher). Supplemental Table 1 summarizes the print list and buffers ...
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bioRxiv - Biochemistry 2022Quote: Purified PPARγ AF-1 D33C+Q121C mutant protein at 50 µM was labeled with Alexa Fluor 488 and Alexa Fluor 647 maleimide dyes (Thermo Fisher) in 20 mM sodium phosphate pH 7.2 ...
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bioRxiv - Molecular Biology 2023Quote: ... 30% acetonitrile (v/v) was added to the digested protein and then labeled using a TMT isobaric mass tagging kit (ThermoFisher Scientific). The TMT reaction was performed for 1 h according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Labeled protein was filter-dialyzed against 10 L of PBS using a 3K-CutOff Slide-A-Lyzer™ Dialysis Cassette (ThermoFisher). Labeling quality was assessed by Mass Spectrometry as previously described [46].
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bioRxiv - Immunology 2023Quote: ... The monomer form of the antigens was biotinylated and the subsequent tetramerization of these proteins was done with FITC-labeled avidin (Invitrogen, A821), in brief ...
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bioRxiv - Molecular Biology 2024Quote: ... FAM labeled TTT probe and TET labeled TATA Binding Protein (TBP) probe were obtained from IDT and used with TaqMan Fast Master Mix (Thermo Fisher) on an Applied Biosystems QuantStudio 12k Flex Real-Time PCR System ...
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bioRxiv - Cancer Biology 2019Quote: ... Positive phage clones were verified for preferential binding to plate-bound Fc-iNKG2D.YA or Fc-iNKG2D.AF versus Fc-wtNKG2D by spot ELISA and bound phage detected with biotinylated M13 phage coat protein monoclonal antibody E1 (Thermo Fisher # MA1-34468) followed by incubation with streptavidin-HRP.
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bioRxiv - Biochemistry 2023Quote: ... Phage pools representing the libraries were cycled through rounds of binding selections with EPHA2-FN2-Fc fusion protein immobilised on Maxisorp Immuno plates (ThermoFisher, #12-565-135), as described previously [21] ...
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bioRxiv - Microbiology 2020Quote: ... cells were exposed to Oregon green 488 (OG488)-labeled and tetramethylrhodamine (TMR)-labeled dextran (Invitrogen) (10 kDa ...
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bioRxiv - Cell Biology 2023Quote: ... The secondary antibodies were Alexa Fluor 488-labeled or 594-labeled (Invitrogen, USA, A32731, A32744). DPIA (5 μg/ml ...
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bioRxiv - Synthetic Biology 2022Quote: ... while the perfluorinated ether FC-770 and the perfluorinated amine FC-3283 were obtained from Acros Organics (Geel, Belgium). Ethanol (96% vol. ...
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bioRxiv - Biochemistry 2022Quote: Recombinant hItln1 and MBL were characterized by western blotting using monoclonal mouse anti-MBL2 antibody (3E7; ThermoFisher Scientific MA1-40145; 1:50) and polyclonal rabbit anti-hItln1 antibody (Proteintech 11770-1-AP ...
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bioRxiv - Microbiology 2021Quote: ... A mouse monoclonal anti-HSV 45-kDa protein (scaffolding protein) antibody (Serotec) at 1:2000 dilution and Alexa Fluor 568–goat anti-mouse IgG (Invitrogen) at 1:1000 dilution were used as primary and secondary antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... were then subjected to preclearing with 20 μL of either magnetic protein G (for Rabbit antibodies) or Protein A (for Mouse antibodies) Dynabeads (Invitrogen) for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... and Alexa-488 or Alexa-594 labeled anti-mouse IgG or anti-rabbit IgG secondary antibodies were from Invitrogen (Carlsbad, CA). Mounting medium for fluorescence with DAPI staining was purchased from Vector Laboratories (Burlingame ...
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bioRxiv - Immunology 2019Quote: AF647 and biotin-conjugated anti-mouse FCRL6 (1C3 and 3C1) were generated and labeled by our laboratory (Invitrogen and Thermo Scientific). Anti-VH11 (3H7 (Rowley et al. ...
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bioRxiv - Immunology 2019Quote: AF647 and biotin-conjugated anti-mouse FCRL6 (1C3 and 3C1) were generated and labeled by our laboratory (Invitrogen and Thermo Scientific). Anti-VH11 (3H7 (Rowley et al. ...
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bioRxiv - Genetics 2021Quote: ... or a mouse anti-HA (MBL, M180-3, 1: 200) antibody followed by incubation with fluorescently labeled secondary antibodies (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Alexa Fluor 594-labeled goat anti-mouse IgG (1:500, Cat# A-11032) and Alexa-Fluor-488-labeled anti-rabbit IgG (1:500, Cat# A32731, Thermofisher, USA). The mean fluorescence intensity of antibody immunostaining was measured as reported previously71 using ImageJ.
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bioRxiv - Biochemistry 2020Quote: ... cells were washed with PBS and labeled with secondary donkey-anti-mouse antibody conjugated to Alexa Fluor 568 (ThermoFisher; Cat.No: A10037) at dilution 1:400 in PBS for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... the primary antibody was labeled with the Alexa-488 goat anti-mouse IgG secondary antibody (Invitrogen, Life Science, Eugene OR, USA) and fluorescent foci observed and counted with an Olympus BX41 microscope equipped with an UPlanFI 4× objective and a FITC filter ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary anti-mouse antibodies labeled either with Alexa Fluor® 488 secondary antibodies (green) or Alexa Fluor® 568 (Red) (Invitrogen) were used at 1:500 dilutions ...
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bioRxiv - Cell Biology 2021Quote: ... The coverslips were then labeled at room temperature for 1 h with Alexa Fluor 488–conjugated goat anti-mouse secondary antibody (Molecular Probes), 0.2% saponin ...
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bioRxiv - Microbiology 2022Quote: ... and Alexa Fluor 680 fluorescent dye-labeled secondary anti-mouse antibody from goat (diluted 1:10,000, catalogue number A21057; Thermo Scientific, Germany). Quantification of protein bands was performed with Odyssey V3.0 software for Western blots and with ImageLab (BioRad ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed with TBS and incubated with an Alexa Fluor 488 labeled goat anti-mouse antibody (1:500,Thermo Scientific) in blocking solution and were finally washed with TBS ...
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bioRxiv - Neuroscience 2021Quote: ... cells were probed with mouse and rabbit-specific fluorescent-labeled secondary antibodies (1:200, Alexa Fluor 488 and 633, Life Technologies). Single frame images were collected using the Keyence BZ-X 710 Microscope ...
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bioRxiv - Microbiology 2020Quote: ... The samples were finally incubated with Alexa Fluor 488-labeled donkey anti-mouse IgG secondary antibody (1:1000, Thermo Fisher Scientific) at 37°C for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... three times and plates were incubated with secondary goat anti-mouse Alexa-Fluor-546-labeled antibody at 1:1,000 dilution (Thermo Fisher Scientific) for 2 h at room temperature in the dark ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed with FACS buffer and then incubated with Alexa Flour 488-labeled goat anti-rabbit or goat anti-mouse IgG (Invitrogen, A11034; Invitrogen, A11001) for 1 h at 4 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... secondary antibody staining was performed with Alexa Fluor 568- or 488-labeled detection reagents (goat anti-rabbit, goat anti-mouse antibodies; Molecular Probes) at 1:200 dilution ...
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bioRxiv - Microbiology 2022Quote: Fluorescein-labeled 300 base β-actin-Mouse was synthesized using MAXIscript T7 Transcription Kit (AM1312, Invitrogen by Fisher Scientific, Waltham, MA) and Fluorescein RNA Labeling Mix (11685619910 ...
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bioRxiv - Microbiology 2022Quote: Fluorescein-labeled 300 base β-actin-Mouse was synthesized using MAXIscript T7 Transcription Kit (AM1312, Invitrogen by Fisher Scientific, Waltham, MA) and Fluorescein RNA Labeling Mix (11685619910 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed with PBS (three times, 10 minutes each) and incubated with an Alexa Fluor 488-labeled anti mouse IgG (Life Technologies) secondary antibody for 1 hour at room temperature and protected from light ...
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bioRxiv - Microbiology 2023Quote: ... The biofilms were then washed once and incubated for 1 h at room temperature with an Alexa Fluor 594-labeled goat anti-mouse IgG highly cross-absorbed secondary antibody (Invitrogen, A32742)) [2 µg mL-1] within BSA blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... The coverslips were washed 3 x in PBS and labeled with donkey anti-mouse AlexaFluor 647 (1:1000, A31571, Thermo Fisher), for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then washed three times and incubated with Alexa 488/647-labeled anti-mouse/rabbit secondary antibody (Invitrogen, A21202, A31573) for 1h at RT ...
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bioRxiv - Cell Biology 2023Quote: ... Slides were washed in PBS pH 7.2 and incubated for 1 hour at 35°C with secondary antibodies diluted 1:250 in PBS buffer with 1% BSA (Alexa Fluor 488-labeled mouse anti-rabbit IgG (Molecular Probes)).
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bioRxiv - Bioengineering 2024Quote: ... sections were stained for 1 h at room temperature in a humidified chamber with AlexaFluor-488-labeled donkey polyclonal anti-mouse IgG (1:500, Invitrogen, A21202) and AlexaFluor-555-labeled donkey polyclonal anti-rabbit IgG (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... Translation sites in fixed cells were labeled anti-Flag labeling (FUJIFILM, Wako) followed by Cy3 or Alexa 594 conjugated anti-mouse secondary antibodies (Jackson Immunoresearch and Invitrogen). In live cells ...
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bioRxiv - Microbiology 2022Quote: ... were resuspended in 100 μL of FC buffer containing primary antibodies (mouse anti-Spike S2, Thermo Fisher, Cat #: MA5-35946, 1:500 dilution) (mouse anti-Flag M2 ...
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bioRxiv - Molecular Biology 2020Quote: ... INS1 cells labeled with Bodipy C12 were also immunostained with mouse anti-HSP60 antibody (66-41-1-lg, Protein Tech) and Alexa Fluor 488 goat anti-Mouse IgG (A11017, Invitrogen) both at 1:300 ...
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bioRxiv - Immunology 2022Quote: ... the wells were incubated with PBS (control) or serially-diluted irrelevant mouse IgG (anti-green fluorescence protein of mouse IgG, ThermoFisher) or irrelevant rabbit IgG (anti-green fluorescence protein of rabbit IgG ...
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bioRxiv - Microbiology 2021Quote: ... For immunofluorescence analysis the cells were fixed as described above and stained with a monoclonal mouse antibody against the viral capsid proteins followed by staining with a goat anti-mouse IgG Alexa Fluor 488 (Invitrogen).
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bioRxiv - Plant Biology 2020Quote: ... Dynabeads M280 Sheep anti-mouse IgG or Dynabeads Protein G (Thermo Fisher Scientific) were used for immunoprecipitation ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... pre-coated with laminin mouse protein at 2μg/cm2 (Thermo Fisher Scientific, 23017015) and maintained in the culture medium with 2% CO2 (24) ...
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bioRxiv - Microbiology 2020Quote: ... Polyclonal antibodies were purified from mouse sera using Protein A Sepharose-4B (Thermofisher) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... the culture dish is coated with laminin mouse protein (20μg/mL, Gibco, 23017015) for another 24 hours and wash three times with water to remove redundant laminin mouse protein ...