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Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... the magnetic beads were resuspended in 10.0625 μL ROX/Hi-Di (0.0625 μL of ROX 350 ladder [Applied Biosystems] in 10 μL of Hi-Di formamide [Applied Biosystems]) and incubated at room temperature for 20 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Cry1 pcDNA4A-myc-his and Cry2 pcDNA4A-myc-his plasmids were double digested with NotI and XhoI FastDigest enzymes (Thermo Scientific) and used as host plasmids ...
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bioRxiv - Biochemistry 2023Quote: Plasmids of pET28a-his TEV-glnR or pET28a-his TEV-glnR derivative was firstly transformed into expression strain BL21(DE3) (Invitrogen, Inc.). then single colonies of the positive transformants were inoculated and amplified with 5 L LB broth supplemented with 50 μg/ml kanamycin at 37 °C with shaking ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies used for human cells are mouse anti-human CD3 conjugated to APC (Thermo Fisher, 47-0036-42), mouse anti-human CD8 conjugated to FITC (BD Biosciences ...
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bioRxiv - Cell Biology 2022Quote: Human cerebral organoids are generated from human induced pluripotent stem cells (iPSCs) which were purchased from GibcoTM (Gibco by Thermofisher ...
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bioRxiv - Bioengineering 2023Quote: ... primary human T cells were thawed and activated with anti-CD3/CD28 Human T-Expander Dynabeads (Thermo Fisher) at a 3:1 bead to cell ratio ...
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bioRxiv - Synthetic Biology 2023Quote: ... primary human T cells were thawed and activated with anti-CD3/CD28 Human T-Expander Dynabeads (Thermo Fisher) at a 3:1 bead to cell ratio ...
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bioRxiv - Cell Biology 2020Quote: All cell lines were cultured in DMEM media supplemented with sodium pyruvate and 10% Hi-FBS (Life Technologies). To express protein constructs in living cells ...
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bioRxiv - Cell Biology 2024Quote: LZTR1WT and LZTR1L580P were expressed as C-terminal His-tagged proteins in Expi-293F cells (Thermo Fisher Scientific). pcDNA3.1-LZTR1-Myc-6xHis plasmid was a gift from Jens Kroll (Heidelberg University and German Cancer Research Center (DKFZ-ZMBH Alliance)).56 The LZTR1L580Pvariant was introduced into the plasmid by site-directed mutagenesis as previously described.11 Cells were transfected using ExpiFectamine 293 Reagent (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: His-CLASP1 protein was expressed in baculovirus-infected Sf9 insect cells using the Bac-to-Bac system (Invitrogen). After the first amplification ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were transfected with a pMT Puro His-PC-SNAP-Elp3 vector using ExpiFectamine™ Sf(A389l5, Gibco) using manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2024Quote: Drosophila S2 cells were transfected with the indicated plasmids or blank pMT-V5-His vector (Invitrogen cat#V412020) using Effectene transfection reagent (Qiagen cat#301427) ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies like goat anti-mouse IgG/IgM Alexa flour 488/543 (A11029; Thermo Fisher Scinetific; 1:2000), goat anti-Rabbit Alexa Flour 546 (A11003 ...
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bioRxiv - Neuroscience 2020Quote: ... IA) with the top ranking promoter-like elements altered by synonymous mutations and cloned into pCR4-Blunt (Invitrogen). Bacterial clones were screened by restriction digestion and those with correct patterns were analyzed by DNA sequencing ...
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bioRxiv - Cell Biology 2020Quote: 7000 Monkey kidney fibroblast-like COS-7cells were seeded per well in gelatin-coated 96- well plates (Gibco DMEM ...
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bioRxiv - Biochemistry 2020Quote: ... mouse anti-transferrin receptor (Invitrogen, catalogue number 13–6800; WB 1:1000), rabbit anti-Stim1 (Cell Signalling Technology ...
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bioRxiv - Genetics 2019Quote: ... mouse anti-transferrin receptor (1:500; clone H68.4; #13-6800, Thermo Fisher), and rabbit anti-calnexin (1:250 ...
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bioRxiv - Biophysics 2019Quote: ... the cDNAs of receptors were subcloned into a modified pfastbac1 vector (Invitrogen), which contained an expression cassette with a haemagglutinin (HA ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-progesterone receptor (catalog no. RM-9102-S0, 1:400, Thermo Scientific) and anti-TER-119 (catalog no ...
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bioRxiv - Cell Biology 2022Quote: ... Insulin Receptor β CT-3 mouse monoclonal antibody (1:1000, AHR0271, ThermoFisher), Perilipin-1/PLIN1 D1D8 rabbit monoclonal antibody (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... anti-transferrin receptor (13-6800, Invitrogen; WB,1:1000, IF,1:200), Transferrin 488(T13342 ...
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bioRxiv - Biophysics 2023Quote: Transient expression of receptors was achieved by using Lipofectamine LTX Plus (Invitrogen), transfecting 0.3 pmol plasmid DNA per 6-cm2 dish.
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bioRxiv - Cell Biology 2020Quote: HEK293 cells (CRL-11268™, ATCC®. Gaithersburg, Maryland, US) were cultured in DMEM high glucose (31966-021, Gibco™, Thermo Fisher, UK), supplemented with 10% FBS (16140-071 ...
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bioRxiv - Cell Biology 2020Quote: HEK293 cells (CRL-11268™, ATCC®. Gaithersburg, Maryland, US) were cultured in DMEM high glucose (31966-021, Gibco™, Thermo Fisher, UK), supplemented with 10% FBS (16140-071 ...
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bioRxiv - Genomics 2022Quote: Frozen HEK293 or K562 cell pellets were lysed in 1 mL lysis buffer supplemented with 5 µl protease inhibitor cocktail (Thermo Fisher cat# 87786) and 10 µl Rnase inhibitor (NEB cat# M0314B ...
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bioRxiv - Cancer Biology 2020Quote: ... SK-Hep1, Huh7, MHCC-97H, and HEK293 cells were cultured in DMEM (HyClone, Logan, UT, USA) supplemented with 10% FBS (Gibco, Rockville, MD, USA), 100 U/ml penicillin ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 cells stably expressing CMV:DOR-APEX2 were created as previously described and maintained in 1:1000 G418/Geneticin (Thermo Fisher Scientific, Cat # 10131035) (Lobingier et al ...
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: The plasmids coding for pro-BMP9 and pro-BMP10 were co-transected in equal amounts into HEK293 cells (expi293, Invitrogen/Thermo Fisher Scientific) and after five days ...
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bioRxiv - Immunology 2023Quote: ... Gregoire Lauvau’s lab) and HEK293 Phoenix Ampho Packaging Cells (provided by Prof. Andrew Ward) were cultured in complete DMEM medium (Gibco, Cat#10569-010) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: Human fibroblast cells lines were maintained in DMEM/F12 (Gibco) with 15% FBS (Sigma Aldrich) ...
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: The human neural stem cells (NSCs) were obtained from ThermoFisher/Invitrogen (N7800-100 ...
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bioRxiv - Cancer Biology 2019Quote: ... Human PDA cell lines were cultured in RPMI 1640 (Gibco) containing 10% FBS ...
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bioRxiv - Developmental Biology 2019Quote: Human umbilical cord stem cell derived hiPSC6.233 (Life Technologies, A18945) were grown on Matrigel-coated (MG ...
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bioRxiv - Cancer Biology 2020Quote: ... Human melanoma cell lines were maintained in DMEM (Gibco #11965) supplemented with 10% FBS (Seradigm) ...
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bioRxiv - Bioengineering 2020Quote: ... Human Umbilical Vein Endothelial Cells (HUVEC) (Thermo Fisher Scientific, USA) were cultured in medium M200 (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2020Quote: Human Umbilical Vein Endothelial Cells (HUVECs) (Thermo Fisher Scientific, USA) used for biocompatibility and cell attachment were passages 4-6 ...
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bioRxiv - Bioengineering 2019Quote: Human corneal epithelial cells (HCECs) were purchased from Life Technologies Ltd (Paisley ...
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bioRxiv - Cancer Biology 2020Quote: Human AML cell lines were maintained in RPMI-media (Gibco) supplemented with 10% FBS (Invitrogen ...
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bioRxiv - Cancer Biology 2019Quote: Human PDA cell lines were cultured in RPMI 1640 (Gibco) containing 10% FBS ...
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bioRxiv - Molecular Biology 2020Quote: ... and human dermal microvascular endothelial cells (HdMVECs) (Life Technologies C01125PA) were purchased and used for in vitro assays ...
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bioRxiv - Microbiology 2020Quote: Adult human epidermal keratinocytes (HEKa) cells (Invitrogen, GIBCO®, UK) were used for two-dimensional co-culture experiments ...
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bioRxiv - Microbiology 2020Quote: Adult human epidermal keratinocytes (HEKa) cells (Invitrogen, GIBCO®, UK) were used for two-dimensional co-culture experiments ...
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bioRxiv - Cancer Biology 2020Quote: ... Human cells were then cytospinned with Shandon cytofunnels (Thermo Fisher) onto glass slides using the CytoSpin 4 instrument (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: Human embryonic kidney (HEK29) cells (Life Technologies GmbH, Darmstadt, Germany) were maintained in high glucose Dulbecco’s modified eagle medium (DMEM ...
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bioRxiv - Cell Biology 2020Quote: Human Embryonic Kidney (HEK) 293T cell lines acquired from ThermoFisher Scientific (# R70007 ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK293T human kidney cells (293FT; Thermo Fisher Scientific, #R70007; RRID:CVCL_6911), NHA-PC5 normal human astrocyte cells (a kind gift from Russell Pieper ...
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bioRxiv - Biochemistry 2022Quote: Human embryonic kidney cells (HEK-293A, Life Technologies, Carlsbad, CA) were cultured at 37°C with 5% CO2 in Dulbecco’s minimal essential medium (DMEM ...
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bioRxiv - Bioengineering 2022Quote: Human Umbilical Vein Endothelial Cells (HUVEC) (Thermo Fisher Scientific, USA) were cultured in EBM™-2 Basal Medium (Lonza ...
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bioRxiv - Biochemistry 2022Quote: ... Antibodies were produced in Expi293F cells (embryonal, human kidney, GIBCO). Cells were maintained in Expi293 Expression Medium (GIBCO ...
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bioRxiv - Microbiology 2020Quote: Human cells adapted in suspension (FreeStyle™293-F, Invitrogen) were cultured in serum-free medium (Freestyle™293 Expression Medium ...