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Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for 7 Chloro 9 methyl 3 4 dihydro 2H benzo b oxepin 5 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 1× B-27 (Gibco), 1× N-2 (Gibco) ...
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bioRxiv - Microbiology 2024Quote: ... and Amphotericin B (Gibco). SARS-CoV-2 (Wuhan-Hu-1 isolate ...
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bioRxiv - Immunology 2024Quote: ... B-Mercaptoethanol [55mM] (Gibco), Sodium Pyruvate [1mM] (Life Technologies) ...
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A nepenthesin insert allosterically controls catalysis in the malaria parasite protease plasmepsin VbioRxiv - Microbiology 2022Quote: ... Lysates were then incubated for one hour at 4°C with Dynabeads-Protein A or Dynabeads-Protein G (ThermoFisher) that had been bound to anti-GFP (clone 3E6 ...
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bioRxiv - Immunology 2020Quote: ... One million cells from lung cell suspension were resolved on 4 – 12 % NuPAGE Bis-Tris protein gels (Thermo Fisher), then transferred onto a nitrocellulose membrane (Biorad ...
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Drosophila immune priming to Enterococcus faecalis relies on immune tolerance rather than resistancebioRxiv - Immunology 2022Quote: ... Samples were centrifuged at 10,000 rcf at 4°C for one minute directly into a 1.5mL microcentrifuge tube containing 350uL TriZol (Life Technologies) (schematic in Supplementary Figure 4A) ...
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bioRxiv - Molecular Biology 2024Quote: ... enteroids were pelleted and washed one time with 1X PBS and fixed in 4% (w/v) PFA (Fisher Scientific) for 15 min at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Selection was applied to transfectants for at least 5 passages prior to use: 150 µg/ml hygromycin-B (ThermoFisher) and 1 µg/ml puromycin (ThermoFisher) ...
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bioRxiv - Neuroscience 2022Quote: ... and the pellet was resuspended in 5 mL B-PER™ complete bacterial protein extraction reagent (Thermo Scientific, #89821) supplemented with 2 mM DTT ...
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bioRxiv - Biochemistry 2020Quote: ... buffer B: 0.2% formic acid and 5% DMSO in acetonitrile) in a Dionex Ultimate 3000 LC-system (Thermo Scientific). Peptides were then analyzed using a LTQ Orbitrap Elite mass spectrometer (Thermo Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... Eyes were intravitreally injected using a glass micropipette with 1-5 μl of 0.2% cholera toxin subunit B (CTB) conjugated to Alexa Fluor 488 or 555 (Invitrogen) diluted in 1% DMSO ...
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bioRxiv - Biochemistry 2024Quote: ... Reaction mixtures were adjusted to a total volume of 5 μl (2 μl solution A; 1,5 μl solution B; 0,3 μl RiboLock (40 U/μl, Thermo Scientific); 0,2 μl D-Luciferin (10 mM ...
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bioRxiv - Developmental Biology 2023Quote: ... Human immortalised endometrial epithelial cells (Ishikawa cells) were cultured at 37°C (5% CO2) in DMEM:F12 B-Nut mix (50:50, ThermoFisher) supplemented with 10% (v/v ...
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bioRxiv - Microbiology 2024Quote: ... Flp-In T-REx 293 cell lines with reconstituted PKR were maintained in 5% FBS DMEM supplemented with 50µg/mL hygromycin B (Invitrogen) and 15µg/mL blasticidin ...
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bioRxiv - Biochemistry 2020Quote: ... Peptides were separated using a 180 minutes long gradient ranging from 3-15% solvent B (after optimization with pre-digested HeLa samples from Thermo Fisher). The mass spectrometer was run in positive mode with full MS (m/z = 400-2000 ...
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bioRxiv - Immunology 2022Quote: ... Cells were counted again and resuspended in 3 × 106 cells/mL before adding CC4F-AM (LiveBLAzer FRET-B/G Loading Kit with CCF4-AM, ThermoFisher Scientific). Cells were incubated for 1 hour ...
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bioRxiv - Microbiology 2024Quote: ... The supernatants from the second centrifugation step were then dialysed overnight at 4 °C against ice-cold buffer B (50 mM Tris pH 7.5, 150 mM NaCl) in SnakeSkin dialysis tubing (Molecular weight cutoff 3 kDa, Thermo Fisher Scientific) until the sample reached pH 7.5 ...
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bioRxiv - Cancer Biology 2024Quote: ... Above cells were split every 4-5 days using 5-10-minute incubation with 0.05% trypsin-EDTA (Thermo Fisher) at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... centrifuged at 5 000×g for 5 min at 4°C in SpinX 0,2 µm tubes (Corning, Fisher Scientific) for cell debris and bacterial removal ...
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bioRxiv - Developmental Biology 2021Quote: ... double-stranded RNA was adjusted to 4 μg/μL and mixed with Rhodamine Dextran B dye (20:1) (Thermo Fisher) for visualization of injected volume ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were pelleted at 3000 × g at 4°C for 30 mins and subsequently lysed with B-PER (Thermo Scientific) supplemented with 50 U DNAseI ...
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bioRxiv - Genomics 2019Quote: ... Protein amount was quantified by BCA protein assay reagents (Pierce) and normalized for loading on a 4–12% B-T NuPage gels (Invitrogen). After transfer on nitrocellulose membrane ...
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bioRxiv - Genetics 2021Quote: Total RNA was isolated from the eggs at 24 hpo from a wild-type strain (p50T) and three b-4 mutant strains (e36, e37, and No. 903) using TRIzol (Invitrogen). Reverse transcription was then performed using the oligo (dT ...
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bioRxiv - Immunology 2022Quote: MACS enriched naïve mouse B cells or Cas9 RNP transfected B cells were cultured for 4 days in 200 μL complete RPMI media per well of 96-well plate (Nunc) in the presence of 3 μg/ml anti-CD40 antibody (Biolegend ...
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bioRxiv - Biochemistry 2022Quote: ... Pellets were frozen for an hour at −20°C and resuspended in 4 mL of B-PER Reagent (Thermo Scientific) per gram of cell pellet with 50 mg/mL lysozyme and 250U/uL Benzoase (Sigma Aldrich NV) ...
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bioRxiv - Immunology 2024Quote: ... were irradiated with 80 Gy and co-cultured with primary splenic B cells for 4 days in high glucose DMEM medium (Gibco) supplemented with 10% FBS ...
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bioRxiv - Genetics 2023Quote: ... The eluents were ultrapure water (solvent A) and acetonitrile (ACN, LC-MS Grade, Thermo Scientific, Catalog #A955-4, solvent B), each containing 0.06% Trifluoroacetic Acid (TFA ...
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bioRxiv - Cell Biology 2023Quote: ... 5 ng/mL rh FGF-b, 25 µg/mL rh insulin, 4% FBS (PCS-950-040, ATCC) and penicillin-streptomycin (Gibco). 24-96 hrs after passaging ...
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bioRxiv - Immunology 2023Quote: ... The concentration was measured at NanoDrop One/One (Thermo Fisher Scientific) and the quality (RNA integrity number ...
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bioRxiv - Molecular Biology 2021Quote: ... antibodies overnight at 4°C and then incubated for an additional 3 hours at 4°C with 50 μL DynabeadsTM protein G (Life Technologies, 10004D). Following three washes with 1% Triton-TBS lysis buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... Human naive PSCs were passaged as single cells every 4 days at split ratio 1:3 or 1:4 following dissociation with TrypLE (Gibco 12563-029) for 10 minutes (min ...
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bioRxiv - Developmental Biology 2023Quote: ... Human naive PSCs were passaged as single cells every 4 days at split ratio 1:3 or 1:4 following dissociation with TrypLE (Gibco 12563-029) for 10 minutes at room temperature (RT) ...
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bioRxiv - Cancer Biology 2019Quote: ... and digested for 2h at 37 at 110rpm in RPMI-1640 (Thermo Fisher, with L-glutamine) containing antibiotic-antimycotic solution ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies were subsequently detected with rabbit 647-conjugated secondary antibodies (1:500, 2h incubation; Invitrogen). Slices were mounted on charged microscope slides (Superfrost/Plus ...
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bioRxiv - Microbiology 2023Quote: ... B.1.640.1 and B.1.640.2) were produced in silico (GeneArt, Thermo Fisher Scientific). Spike sequences were then cloned into a phCMV backbone (GeneBank ...
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bioRxiv - Biophysics 2019Quote: 20-nm fluorescent dark red beads samples (Fig. 2d, Supplementary Fig. 5) were prepared using a 5.10−7 dilution of the initial solution (F8783, Thermo Fisher). We performed the dilution in PBS + 5% glucose to match the index of the dSTORM imaging buffer ...
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bioRxiv - Molecular Biology 2019Quote: Huh-7 (human hepatoma) cells were maintained at 37 °C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies) supplemented with 10% (vol/vol ...
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bioRxiv - Cell Biology 2019Quote: ... COS-7 and U2OS cells were grown at 37°C with 5% CO2 in complete DMEM (Thermo Fisher Scientific, USA) containing 10% FBS and 1% L-glutamine ...
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bioRxiv - Plant Biology 2021Quote: ... pistils and young seeds (7 DAP from CT and 5 DAP from HT) were isolated following a protocol for Trizol (Invitrogen). RNA from 26 DAP seeds was isolated using a NucleoSpin RNA Plant and Fungi kit (Macherey-Nagel ...
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bioRxiv - Neuroscience 2022Quote: ... jGCaMP7b-XC or jGCaMP7b-XN and 1 μg cDNA encoding CFP (for labelling the soma area and neurites) were transiently transfected into DIV 5-7 cultured cortical neurons by Lipofectamine 2000 (Invitrogen) with a typical protocol according to the manual ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were extracted from rats aged postnatal day 5-7 and placed in working buffer (Hank’s balanced salt solution (HBSS) (Life Technologies) with 0.5% chromatographically purified bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2022Quote: ... HEK293 and COS-7 cells were maintained at 37 ºC and 5% CO2 and cultured in Dulbecco’s modified Eagle media (Life Technologies) supplemented with 10% FBS (Hyclone ...
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bioRxiv - Molecular Biology 2023Quote: ... Northern blot analysis of U1 snRNA was performed using 5’-radiolabeled DNA probes (Sequence is listed in Supplemental Table 7) in ultrasensitive hybridization Buffer (Thermofisher). The radioactivity signals were analyzed by PhosphorImager (Typhoon FLA 7000).
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bioRxiv - Synthetic Biology 2022Quote: ... 5’ and 3’ RACE ready cDNA was generated from RNA extracted from ~30 pairs of ovaries or testes dissected from 5-7 days-post-emergence (dpe) Liverpool strain adults using Trizol (Invitrogen). Primers LA1076 then nested with LA1352 (5’ ...
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bioRxiv - Microbiology 2024Quote: ... approximately 5 × 105 Huh-7 cells were transfected with 2 μg of purified BAC DNA using Lipofectamine 3,000 (Fisher Scientific) as a transfection reagent ...
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bioRxiv - Biochemistry 2024Quote: ... Liposomes at 5-7 mM were applied to the carbon side of a grid held in a Vitrobot Mark IV (ThermoFisher) chamber kept at 4°C and 100% relative humidity ...
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bioRxiv - Immunology 2023Quote: Lungs were transferred into 7 ml bijoux tubes containing 500 µl digestion mix consisting of 5 mg/ml collagenase I (Gibco) and 10 µg/ml DNase I (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then dissociated using 0.5mM EDTA into a single cell suspension and incubated in 5 μM of cell-permeant 2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) dye (Invitrogen) for 30 min at 37°C in the dark ...
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bioRxiv - Microbiology 2023Quote: ... approximately 5×105 Huh-7 cells were transfected with 2 µg of purified BAC DNA using Lipofectamine 3000 (Fisher Scientific) as a transfection reagent ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were passaged every 5-7 days once they reached about 70-80% confluency using TrypLE cell dissociation reagent (Gibco) and gentle scraping before being replated onto new MEFs ...