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Citations for Thermo Fisher :
1301 - 1350 of 10000+ citations for Urea Nitrogen BUN Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Permeabilization and EdU detection was carried out using the Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s manual ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative RT-PCR was performed using QuantiTect Probe RT-PCR Kit (Quiagen®) in an ABI PRISM 7500 Sequence Detection System (Applied Biosystems). Amplifications were carried out in 25 µL reaction mixtures containing 2× reaction mix buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... after which then genomic DNA was isolated for detection of gene editing efficiency using the GeneArt genomic cleavage assay kit (Invitrogen, catalog #A24372). Hep3Bcas9 cells had high cleavage efficiency and thus was chosen for generating knockout cell lines using CRISPR ...
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bioRxiv - Molecular Biology 2024Quote: ... The hybridization signal from the biotin-labeled probe (listed in Supplementary Table 1) was detected using the Chemiluminescent Nucleic Acid Detection Module Kit (Thermo Fisher Scientific), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... We then compared the performance of the different kits in terms of detection and quantification rates using standard RT-PCR (Applied Biosystems 7500 Fast Instrument, Thermo Fisher) and portable RT-PCR (Magnetic Induction Cycler quantitative PCR (Mic qPCR) ...
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bioRxiv - Cancer Biology 2024Quote: ... qRT-PCR was performed using DyNAmo ColorFlash SYBR Green qPCR Kit in the ABI Quant Studio 5 Sequence Detection System (Life Technologies, USA). The expression of the genes of interest was analyzed by the ΔΔCt method using ATP5G rRNA and GAPDH as internal control genes ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then washed twice with PBS and fixed with 2% PFA before being stained for β-galactosidase activity with the CellEvent™ Senescence Green Detection Kit (Invitrogen) per manufacturer instructions.
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bioRxiv - Cancer Biology 2023Quote: Fixed brain sections were rehydrated and underwent antigen retrieval before being processed for TUNEL assay using the Click-iT™ Plus TUNEL Alexa Fluor™ 647 Assay Kit for In Situ Detection (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The number of SIV copies was normalized to diploid genome cell equivalents co-determined by Q-RT-PCR using the TaqMan™ RNase P Detection Reagents Kit (Applied Biosystems), as described previously (11 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were counterstained using DAPI provided in the detection reagent kits and mounted in ProLong Gold mounting media (P36930, Thermo Fisher Scientific). The images were obtained using an Olympus FV3000 confocal laser scanning microscope with a 40X magnification oil immersion objective ...
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bioRxiv - Immunology 2023Quote: ... Lysates were boiled for 5 minutes (except lysates used for detection of electron transport chain complexes) and protein content was determined using a BCA kit (23225, Thermo Fisher Scientific). Proteins were separated by SDS-PAGE and transferred to a 45 µm PVDF membrane ...
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bioRxiv - Cell Biology 2024Quote: ... All cells were assessed for mycoplasma contamination at monthly intervals using the LookOut Mycoplasma Detection kit (MP0035, Thermo Fisher Scientific, Loughborough, UK).
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bioRxiv - Molecular Biology 2024Quote: TUNEL assays were performed on deparaffinized heart sections using a Click-iT™ Plus TUNEL Assay Kits for In Situ Apoptosis Detection (Invitrogen, C10617), according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2024Quote: Single-molecule fluorescence in situ hybridization (smFISH) for miRNA detection in hippocampal neuron cultures was conducted using the QuantiGene ViewRNA miRNA Cell Assay Kit (Thermo Fisher, QVCM0001) following the manufacturer protocol with minor adjustments ...
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bioRxiv - Physiology 2024Quote: All real-time PCRs were carried out in 96-well plates using ChamQ SYBR qPCR Master Mix kit (Nanjing Vazyme Biotech Ltd., China) in the ABI Prism 7500 Sequence Detection System (Applied Biosystems, USA) at 95°C ...
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bioRxiv - Cancer Biology 2021Quote: ... nigericin (10 μM) and valinomycin (10 μM) by following manufacturer’s protocol from the Intracellular pH Calibration Buffer Kit (Invitrogen).
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bioRxiv - Molecular Biology 2023Quote: ... Twice-purified DNA was eluted into 10 μl of 10 mM Tris-HCl (pH 8.0) and quantified using the Qubit dsDNA HS Assay Kit (Invitrogen) with a Qubit 4 Fluorometer ...
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bioRxiv - Microbiology 2020Quote: ... and plunge-frozen into liquid ethane-propane mix cooled at liquid nitrogen temperatures with a Mark IV Vitrobot(Thermo Fisher Scientific) maintained at room temperature and 70 % humidity.
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bioRxiv - Microbiology 2022Quote: ... they were stored in liquid nitrogen for less than a week before tilt-series collection at the Titan Krios (ThermoFisher Scientific). The final thickness of lamellas was measured at the Titan Krios ...
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bioRxiv - Molecular Biology 2020Quote: ... Grids were blotted for 5 s and plunge-frozen in liquid ethane cooled by liquid nitrogen using a Vitrobot MarK IV (Thermo Fisher) at 8 °C with 100 percent humidity ...
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bioRxiv - Genomics 2019Quote: ... This was achieved by homogenisation (within a pestle and mortar under liquid nitrogen) in TRIzol Reagent (Thermo Fisher Scientific Ltd, UK) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: ... FTIR spectra were recorded on a Nicolet 6700 FT-IR spectrometer equipped with a liquid nitrogen cooled mercury-cadmium-telluride detector (ThermoFisher Scientific), with a spectral resolution of 4 cm−1 ...
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bioRxiv - Cell Biology 2019Quote: ... Both DifcoTM YPD broth and DifcoTM Yeast Nitrogen Base (YNB) w/o Amino Acids and Ammonium Sulfate were purchased from Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: ... The fungal mat was then collected and homogenized in liquid nitrogen and total RNA was extracted using trizol reagent (Invitrogen, USA) followed by phase separation with chloroform and subsequent precipitation of RNA using isopropanol ...
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bioRxiv - Biochemistry 2021Quote: ... Cryo-EM grids were stored in liquid nitrogen until data were collected with a 300 kV Titan Krios G3i (Thermo Fisher) equipped with a K3 direct electron detector (Gatan) ...
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bioRxiv - Plant Biology 2021Quote: ... These tissue samples were then crushed in liquid nitrogen and RNA was isolated using TRIzol reagent72 according to the protocol provided by the manufacturer (Invitrogen, USA).
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bioRxiv - Microbiology 2021Quote: ... The grids were blotted and flash-frozen in liquid ethane cooled by liquid nitrogen with a Vitrobot apparatus (Mark IV, ThermoFisher Scientific). The grids sample quality was verified with an FEI Talos Arctica 200-kV electron microscope (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: The concentrations and isotopic compositions of nitrogen and carbon in different sediment fractions were determined simultaneously using a Flash-EA 1112 (ThermoFisher Scientific) coupled to an isotope ratio mass spectrometer (IRMS ...
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bioRxiv - Biophysics 2020Quote: ... Sample grids were flash-frozen in liquid ethane cooled to liquid nitrogen temperatures on holey carbon grids using a vitrobot automated virification system from Thermo Fisher. Data were collected on the Titan Krios microscope housed at the Electron Imaging Center for Nanomachines (EICN ...
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bioRxiv - Molecular Biology 2020Quote: ... which were then blotted for approximately 8 s and immediately plunged in 1:2 ethane:propane (Carbagas) at liquid nitrogen temperature using a Vitrobot (Thermo Fisher Scientific). The Vitrobot chamber was kept at 4 °C and 100% humidity during the whole procedure.
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bioRxiv - Molecular Biology 2020Quote: ... 4 μl of sample was applied to a charged grid and blotted and plunge-frozen in nitrogen-cooled liquid ethane using a Vitrobot Mark IV robot (Thermo Fisher) set to blot force 20 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Excess sample was immediately blotted off and vitrification was performed by plunging the grid into liquid nitrogen-cooled liquid ethane at 100% humidity and 4°C using an FEI Vitrobot Mark IV (Thermo Fisher). The data were collected on a Thermo Fisher Titan Krios electron microscope (Astbury Biostructure Laboratory ...
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bioRxiv - Physiology 2020Quote: ... Tissues were frozen in liquid nitrogen and homogenized in lysis buffer (1% Triton X-100 in PBS) using Bead Mill 24 (Fisher Scientific). Lysates were centrifuged and supernatants were used for ELISAs ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were then stored at -140 °C in liquid nitrogen (CryoPlus Storage Systems model 7402, ThermoFisher Scientific, Hanover Park, IL, USA).
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bioRxiv - Biophysics 2020Quote: ... which were blotted for 3.5 s and immersed in liquid ethane cooled by liquid nitrogen using Vitrobot (Mark IV, Thermo Fisher Scientific). The grids were exposed through Titan Krios operating at 300 kV equipped with Gatan K3 Summit detector and GIF Quantum energy filter in super-resolution mode (81,000× magnification) ...
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bioRxiv - Biophysics 2020Quote: ... Grids were blotted for 5 s and plunge-frozen in liquid ethane cooled by liquid nitrogen using a Vitrobot MarK IV (Thermo Fisher) at 8 °C with 100 percent humidity ...
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bioRxiv - Immunology 2020Quote: ... Grids were plunged into liquid nitrogen cooled ethane. Movies were collected using SerialEM (Schorb, Haberbosch et al. 2019) on a Titan Krios (Thermo Fisher) operating at 300 kV ...
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bioRxiv - Cancer Biology 2022Quote: The pancreatic tumor and normal pancreatic tissue samples were ground in liquid nitrogen and homogenized fully in TRIzol reagent (Life Technologies) and liquid nitrogen ...
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bioRxiv - Biophysics 2022Quote: ... Grids were plunge-frozen into a 37:63 (v/v) mixture of ethane/propane cooled to liquid nitrogen temperature using a Vitrobot Mark IV instrument (ThermoFisher Scientific) at 95% humidity and 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... Grids were again stored in liquid nitrogen until data could be collected using a 300 kV Titan Krios (Thermo Fisher Scientific), also equipped with a K3 detector ...
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bioRxiv - Genetics 2022Quote: ... Mouse brain tissues were ground into powder in liquid nitrogen with mortar and pestle followed by extraction of total RNA by TRIzol (Invitrogen, 15596018). Total RNA extracted from Sik3+/+ ...
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bioRxiv - Neuroscience 2024Quote: ... blotted for 4.5 s and flash-frozen in liquid ethane cooled by liquid nitrogen with Vitrobot (Mark IV, Thermo Fisher Scientific).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The eluted samples were further concentrated to 1 mL under a gentle stream of nitrogen (40°C, Reacti-vap III, Thermo Scientific) and transferred to HPLC vials ...
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bioRxiv - Immunology 2024Quote: Mouse heart tissue that was flash frozen in liquid nitrogen then stored in -80°C was processed using TRIzol-chloroform (Invitrogen, 15596026), stainless steel beads (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... 3 μl protein sample at 1 mg/ml concentration was applied to the grids and plunge frozen into liquid ethane cooled in liquid nitrogen using an FEI Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... and vitrified by directly plunging in liquid ethane (cooled by liquid nitrogen) using Vitrobot Mark IV (Thermo Fisher Scientific, Waltham, Massachusetts) at 100% humidity and 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... 300 mesh Au grids and plunge-frozen in liquid nitrogen-cooled liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) with a blot force of 1 and followed by 5 s blot time ...
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bioRxiv - Biochemistry 2024Quote: ... The grid was plunge-frozen in liquid ethane cooled to the temperature of liquid nitrogen with a Vitrobot Mark IV instrument (Thermo Scientific). A Krios G3i microscope (Thermo Scientific ...
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bioRxiv - Developmental Biology 2023Quote: Myocardial ventricular lysates were obtained from ventricular tissue flash frozen on liquid nitrogen and prepared in RIPA buffer (Thermo Fisher, #89900) with 1X HALT protease and phosphatase inhibitor cocktail (Thermo Fisher ...
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bioRxiv - Synthetic Biology 2023Quote: ... 5 g/L ammonium sulphate (Fisher Chemical™) and 1.7 g/L yeast nitrogen base without amino acids (Thermo Scientific™) and was supplemented with 2% (w/v ...