Labshake search
Citations for Thermo Fisher :
1201 - 1250 of 10000+ citations for Urea Nitrogen BUN Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pathology 2020Quote: Three separate 10 µL RT-qPCR reactions were set up in a 384-well Barcoded plate (Thermo Scientific) for either the N1 ...
-
bioRxiv - Cell Biology 2019Quote: ... 15 mg/mL) and photon counts were obtained after 10 minutes using a plate reader (VarioSkan Lux, Thermofisher), with 1 second acquisition per well ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... We ran 10 plates (384-well format) on QuantStudioTM 12K Flex Real-Time PCR System (Thermo Fisher Scientific). Each 10 μL reaction contained 200 nM of each primer ...
-
bioRxiv - Cell Biology 2021Quote: ... Suspended myofibers were cultured in 60 mm horse serum-coated plates in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS; Invitrogen), 2% chicken embryo extract (Accurate Chemical ...
-
bioRxiv - Systems Biology 2021Quote: ... Mixtures were supplemented with Alexa-488 at 10 µM and plates were sealed with adhesive qPCR seals (ThermoFisher).
-
bioRxiv - Biochemistry 2022Quote: ... 5 × 105 landing pad cells expressing hACE2 with mNG21-10 were seeded in 6-well plates (Fisher Scientific). The cells were then then incubated at 37 °C with 5% CO2 for 15 mins to allow seeding ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293-T cells were seeded into 10 cm plates with DMEM containing 0.5 mg/mL geneticin G418 (Thermofisher). Once the cells reached approximately 80% confluence ...
-
bioRxiv - Microbiology 2022Quote: ... 4×104 conidia of each isolate was evenly spread on a solidified RPMI plate (1.5% agar) and 10 μl of 0.8 mg/mL voriconazole or 3.2 mg/mL itraconazole (Acros Organics) was added to a Whatman 6 mm antibiotic assay disc which was placed in the middle of the plate ...
-
bioRxiv - Neuroscience 2019Quote: ... The plate was then washed and 100 μL of CSF (diluted 1:10 in Blocker Casein (Thermo Scientific) in Tris-buffered saline ...
-
bioRxiv - Cell Biology 2019Quote: 80% confluent 10 cm plates of Plat-E cells were transfected by using Lipofectamine 2000 (Thermo Fisher Scientific) with 10 μg retrovirus vectors in additional 1.5 ml Opti-MEM (Thermo Fisher Scientific) ...
-
bioRxiv - Pathology 2021Quote: ... Hepatocytes were allowed to attach for 90 minutes on collagen-coated plates in RPMI containing 10% FBS (Gibco), followed by overnight starvation in serum-free medium before experiments (Lipogenesis and β-oxidation assay).
-
bioRxiv - Synthetic Biology 2021Quote: ... 10 µL was then added to each well of an assay ready plate using a Multidrop Combi (ThermoFisher). Percent inhibition was calculated on a scale of 0% (i.e. ...
-
bioRxiv - Molecular Biology 2021Quote: ... 3 x 106 WT HEK293T cells were plated in 10 cm plates and transfected using Lipofectamine 2000 (Invitrogen) with the following plasmids expressing V5-SBP fusions ...
-
bioRxiv - Microbiology 2021Quote: ... A 10 μL drop from each dilution was transferred to a Mueller-Hinton plate (Thermo Fisher, Waltham, MA) and incubated overnight in ambient air at 35°C (58) ...
-
bioRxiv - Immunology 2020Quote: ... For poly(I:C) stimulation cells were transfected in 6-well plates with 10 μg polyI:C using Lipofectamine 2000 (Invitrogen) for 2 h before imaging ...
-
bioRxiv - Genomics 2022Quote: ... Plates were incubated for 10 minutes with PBS with Hoechst 33342 (1:500 dilution) (#134406, Thermo Fisher Scientific) and then washed 3x with PBS ...
-
bioRxiv - Cell Biology 2024Quote: We seeded HEK293T cells at 2.5 × 10^6 cells per 15 cm plate in DMEM (Thermo Fisher 11965118), enriched with 10% FBS (Atlanta Biologicals S11150H) ...
-
bioRxiv - Microbiology 2024Quote: ... 1 in 10 serial dilutions solutions were created using sterile PBS in a 96 well plate (ThermoFisher Scientific). Bacterial counts (CFU/mL ...
-
bioRxiv - Cell Biology 2024Quote: ... were cultured in tissue culture treated plates containing Dulbecco’s Modified Eagle’s Medium (DMEM) (ATCC # 30-2002) containing 10% fetal bovine serum (Gibco) until 60% confluent after which cells were transfected using Lipofectamine 2000 (Invitrogen # 11668019 ...
-
bioRxiv - Developmental Biology 2023Quote: Mouse ESCs from 10 cm culture plates were harvested and disassociated using Trypsin EDTA (Thermo Fisher Scientific, 25200056), washed twice in PBS (cells pelleted by centrifugation 300 rpm ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Three 10 μL aliquots of each reaction mixture were transferred to a 384-well plate (Thermo Scientific 142761), covered with a seal (VWR 60941-078) ...
-
bioRxiv - Immunology 2023Quote: ... 10% H2/N2 (BOC) for 3 days on blood agar plates containing 7% laked horse blood (Thermo Scientific) and the Campylobacter selective supplement “Skirrow” containing the antibiotics trimethoprim ...
-
bioRxiv - Developmental Biology 2023Quote: ... The Six2+ cells were directly FACS sorted into a 96 well plate supplemented with 10% FBS/ Dulbecco’s modified Eagle’s medium (DMEM,Gibco) in the presence of 4 µM DMSO (Thermo) ...
-
Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progressionbioRxiv - Cancer Biology 2024Quote: ... Cancer cells (5 × 103 cells) were seeded in 96-well plates in DMEM supplemented with 10% FBS (Gibco) and 1% antibiotic-antimycotic (Gibco) ...
-
bioRxiv - Cell Biology 2024Quote: ... Horizon Discovery) using the DharmaFECT reagent (54 μL/10 cm plate) in OPTI-MEM reduced serum media (ThermoFisher) according to the manufacturer’s instructions (Horizon Discovery) ...
-
bioRxiv - Microbiology 2023Quote: ... qPCR was conducted in 384-well plates using the SensiFAST SYBR Lo-ROX Kit (Meridian Bioscience) on the QuantStudio 5 (384-well plate) qPCR cycler (Applied Biosystems, Foster City, CA, USA). The qPCR program encompassed 40 cycles ...
-
bioRxiv - Immunology 2022Quote: ... and IL-10 Mouse Uncoated ELISA Kit (88-7105-86, Invitrogen), respectively ...
-
bioRxiv - Bioengineering 2021Quote: The Neon™ Transfection System 10 μL kit (Thermo Fisher Scientific) was used to transfect K562 and K562 BFP reporter cells according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2021Quote: Cells were electroporated with the Neon Transfection 10 μL Kit (Invitrogen) per the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2020Quote: ... 10 μL Maxima SYBR Green brilliant PCR amplification kit (Thermo Scientific), 5 uL forward and reverse primer mix (2 nM) ...
-
bioRxiv - Genetics 2023Quote: ... along with Neon Transfection System 10 μL Kit (Cat#MPK1096 Invitrogen). The reprogramming cocktail was prepared by adding 10 μL buffer T ...
-
bioRxiv - Microbiology 2019Quote: ... A 30-fold molar excess of the crosslinker disuccinimidyl dibutyric urea (50 mM stock solution in dimethyl sulfoxide, Thermo Scientific) was added to the protein solution and incubated at 37 °C for 1 h ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... and the resulting 15 uL mixture was separated by gel electrophoresis with a 15% polyacrylamide TBE-urea gel (Invitrogen EC68852BOX) at 200 V for 30 minutes ...
-
bioRxiv - Cancer Biology 2019Quote: Total 20µg of RNA from A375 and A375/211 cells was separated on 15% denaturing polyacrylamide NovexTM TBE urea gel (Invitrogen), blotted on to BrightStarTM-plus positively charged nylon membrane (Invitrogen ...
-
bioRxiv - Microbiology 2019Quote: ... RNA fragments between 20 and 40 bp were isolated by size excision from a denaturing polyacrylamide gel (15%, TBE-Urea, 65 min., 200 V, ThermoFisher). RNA fragments were dephosphorylated using T4 polynucleotide kinase (New England Biolabs ...
-
bioRxiv - Microbiology 2020Quote: ... and RNA integrity was verified by running 50 ng of total RNA on a Novex 6% TBE-urea polyacrylamide gel (Invitrogen).
-
bioRxiv - Cancer Biology 2021Quote: ... The pellet was re-suspended in 8M urea/50mM Tris pH8 and protein concentration was determinate with Qubit Protein Assay (Invitrogen).
-
bioRxiv - Neuroscience 2022Quote: Dry protein pellets of both ovary and hemolymph samples were dissolved and reduced in 8 M urea (Fisher Scientific, 45000234)/70 mM ammonium bicarbonate (Fisher Scientific ...
-
bioRxiv - Cell Biology 2022Quote: ... alkylated using iodoacetamide in 8M urea denaturation buffer (50mM Tris-HCl, pH 8) and digested overnight in 50mM ammonium bicarbonate using trypsin (ThermoFisher) at 37°C ...
-
bioRxiv - Molecular Biology 2019Quote: ... one cDNA fraction corresponding to 70–200 nt was gel purified after 6% TBE-urea gel electrophoresis (Thermo Fisher Scientific). After the final PCR step ...
-
bioRxiv - Genomics 2019Quote: ... 12.5μL of extracted RNA in DEPC treated water was mixed with 12.5μL of Novex TBE-Urea Sample Buffer (2X) (Thermo Fisher Scientific) and denatured for 3 mins at 70°C before snap cooling on ice ...
-
bioRxiv - Biochemistry 2019Quote: ... RNAs after hDcp2 treatment were resolved electrophoretically on denaturing 15% acrylamide / 7 M urea 1xTBE gel stained with SYBR Gold (Invitrogen) and visualized using a Typhoon FLA 9500 (GE Healthcare).
-
bioRxiv - Microbiology 2020Quote: ... and RNA integrity was verified by running 50 ng of total RNA on a Novex 6% TBE-urea polyacrylamide gel (Invitrogen).
-
bioRxiv - Microbiology 2020Quote: ... and the efficiency of rRNA depletion was verified by running 50 ng of total RNA on a Novex 6% TBE-urea polyacrylamide gel (Invitrogen).
-
bioRxiv - Genomics 2021Quote: ... RNAs of interest were size-selected and purified using Novex-TBE Urea gels according to the manufacturer’s instructions (Life Technologies). Final quality control of the purified RNAs was performed using the Agilent 2100 Bioanalyzer with the RNA 6000 Pico LabChip Kit (Agilent ...
-
bioRxiv - Biochemistry 2022Quote: ... Eluates were diluted to 2 M urea in 50 mM ammonium bicarbonate and digested with 1 μg of trypsin (ThermoFisher) overnight at 37°C ...
-
bioRxiv - Immunology 2022Quote: ... resuspended in solubilization buffer (9M Urea containing 1% DTT and 2%Triton X and NuPAGE lithium dodecyl sulfate sample buffer 4X (Invitrogen) at a 3:1 ratio) ...
-
bioRxiv - Molecular Biology 2022Quote: ... 20 μg total RNA was run on 20% (w/v) acrylamide/8M urea gels with a 32P-labeled Decade marker (Ambion), and then transferred onto Hybond-N membranes (Amersham Pharmacia Biotech) ...
-
bioRxiv - Molecular Biology 2024Quote: ... The lysis buffer was exchanged for the reaction buffer (4M Urea+ lysis buffer) using the Zeba Spin Desalting Columns (ThermoFisher). Following buffer exchange the lysate protein concentration was measured using the Qubit protein assay kit and the protein concentration adjusted to 2 mg/ml using the reaction buffer ...
-
bioRxiv - Neuroscience 2022Quote: ... samples were diluted to 1M urea with a blend of 468 μL of 50 mM ammonium bicarbonate (32) and 7 μg of Trypsin (ThermoFisher). The samples were subsequently incubated overnight at 25°C for digestion with trypsin ...