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Citations for Thermo Fisher :
1301 - 1350 of 10000+ citations for Recombinant Human TNFRSF18 Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Recombinant E protein was produced by transient transfection of Expi293F cells using a ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell supernatants were harvested 4 days after transfection and then concentrated before exchange into 2× PBS at pH 6.5 and finally into 2× PBS at pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: Ribonucleoprotein complexes containing 3.9 picomoles of ATF3 sgRNA guides and 3 picomoles of purified recombinant Cas9 protein (Synthego) were transfected into HEK293T cells using Lipofectamine CRISPRmax (Invitrogen). 72 hours post transfection ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were sonicated and recombinant proteins purified in a single step on Ni-agarose column according to manufacturer instructions (Invitrogen). Protein samples were dialyzed against PBS with 5 mM MgCl2 and 0.1% glycerol for 2h and used immediately in experiments ...
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bioRxiv - Microbiology 2019Quote: ... Stable cell lines expressing the recombinant protein were initially maintained in complete medium comprising of serum free medium Sf-900 II SFM (ThermoFisher) complemented with 10% v/v foetal bovine serum (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... recombinant spike protein and ACE2 was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Microbiology 2020Quote: A baculovirus expression system was used for the expression of DUB2 recombinant protein in sf9 insect cells as described in the Bac-to-Bac Baculovirus Expression protocol (Invitrogen). The gene was first cloned into the PFastBacNKI-his-3C-LIC vector using Ligation Independent Cloning (LIC) ...
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bioRxiv - Microbiology 2019Quote: ... bacteria resuspended to OD660 0.3 were incubated with 1 µg of recombinant protein in the presence of 1x protease inhibitors (Pierce, Thermo Scientific) in sterile 1.5 ml microcentrifuge tubes with gentle end-over-end mixing for 30 min at 25°C.
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bioRxiv - Immunology 2021Quote: For competitive ELISA recombinant hACE2 protein were immobilized (400 ng per well) on immunoplates (Nunc MaxiSorp, Thermo Fisher Scientific, USA). RBD-specific nanobodies were mixed with HRP-conjugated recombinant RBD protein (0.2 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... LPS-primed THP-1 cell lysates or purified recombinant NLRP3 protein were pre-cleaned using streptavidin magnetic beads (88816, ThermoFisher) to remove nonspecific binding ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant Breg and GFP protein was labeled with membrane non-permeable biotin according to the manufacturer’s instructions (Thermo Fisher Scientific).
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bioRxiv - Genomics 2022Quote: ... Recombinant protein was expressed in 8L culture of Spodoptera frugiperda SF9 cells using the Bac-to-Bac system (Thermo Fisher). Cells were cultured at 27°C ...
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bioRxiv - Plant Biology 2022Quote: ... NiHa-OSR and NoFa-ICL: Fractions containing recombinant protein were pooled and dialyzed using a Slide-A-Lyzer (ThermoFisher Scientific) cassette (20 kDa MWCO ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant GFP and RPH1 proteins were enriched and purified by affinity chromatography using Ni-NTA Superflow resin (Thermo Scientific). Protein concentrations were calculated based on a standard curve created using bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2023Quote: Plasmids for recombinant protein expression were constructed using mega primer insertion PCR [64] using the plasmid Champion pET303/CT-His vector (Invitrogen). The double-stranded DNA inserts (Integrated DNA Technologies ...
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bioRxiv - Molecular Biology 2023Quote: Recombinant Rpb4/Rpb7-6His protein (5 μg) was incubated in a 50 μL slurry of HisPur Cobalt Resin (Thermo Scientific) in binding buffer (20 mM Tris [pH 7.5] and 50 mM NaCl ...
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bioRxiv - Immunology 2021Quote: ... Transduction efficiency was determined between days 8-10 of culture using a polyclonal goat anti-human IgG Fc PE (ThermoFisher Scientific, #12-4998-82) and the synaptic transfer to BSLB addressed immediately.
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a Fc part (hinge-CH2-CH3) of human IgG1 for purification was inserted into pFUSE-mIgG1-Fc2 vector (Invitrogen, Thermo Fisher Scientific, MA, USA). The construct (RBD-huFc ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were then washed once with 150 μL 1% BSA/PBS and then incubated with 50 μL Goat anti-Human IgG Fc secondary antibody PE (ThermoFisher, cat #12-4998-82) at a final concentration of 2.5 μg/mL in 1% BSA/PBS ...
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bioRxiv - Immunology 2023Quote: Cells were stained for viability (Supplementary table 1 and Supplementary table 4) and blocked for CD16/32 with anti-human FC Receptor binding inhibitor (ThermoFisher Scientific, 14-9161-73) in PBS for 15 minutes at room temperature (Spn ...
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bioRxiv - Biophysics 2020Quote: ... 20% fetal calf serum (FCS) (Invitrogen), 0.1 mM non-essential amino acids ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% FCS (Life Technologies) and kept on ice ...
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bioRxiv - Molecular Biology 2021Quote: ... both supplemented with 10% FCS (Invitrogen), 2 mM glutamine (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... 10% FCS and HEPES 15mM (Gibco). For inhibitor/activator treatments samples were dissected at E11.5 or E11.75 ...
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bioRxiv - Neuroscience 2020Quote: ... After addition of 20% FCS (Invitrogen), the tissue was mechanically dissociated and filtered through a 35µm nylon strainer (Falcon) ...
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bioRxiv - Cancer Biology 2019Quote: ... supplemented with 10% FCS (Gibco®). Cells (2.5×104/ml ...
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bioRxiv - Cancer Biology 2019Quote: ... supplemented with 10% FCS (Gibco®) and 1% penicillin/streptomycin (Gibco® ...
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bioRxiv - Bioengineering 2021Quote: ... supplemented with 10% FCS (Gibco #10437028) and 50U/mL penicillin/streptomycin ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 10 % dialyzed FCS (Gibco), 201.3 μM Met (63-68-3 ...
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bioRxiv - Molecular Biology 2022Quote: ... 10% fetal calf serum (FCS, Invitrogen) and 1% penicillin/streptomycin (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... and supplemented with 10% FCS (Gibco) (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... with 10% heat-inactivated FCS (Gibco) and penicillin/streptomycin (Gibco) ...
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bioRxiv - Genetics 2022Quote: ... 15% FCS (Cat. #10500064, Life Technologies); 20 µl PHA-L (Cat.# 00-4977-03 ...
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bioRxiv - Cancer Biology 2022Quote: ... 15% fetal calf serum (FCS, Invitrogen), 450 μM monothioglycerol ...
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bioRxiv - Microbiology 2022Quote: ... and NPM1 (FC-61991, Life Technologies). Secondary antibody (A11029 or A11036 ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... and 10% heat-inactivated FCS (Invitrogen)) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... (hi-FCS, Gibco, Life Technologies, UK), were incubated (37°C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... (hi-FCS, Gibco, Life Technologies, UK), were incubated (37°C ...
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bioRxiv - Cancer Biology 2019Quote: ... supplemented with 10% FCS (Life technologies). All media were supplemented with 2 mM L-glutamine ...
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bioRxiv - Biochemistry 2019Quote: ... and supplemented with 10% FCS (Gibco). Cells were split on reaching 80% confluency.
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bioRxiv - Biophysics 2019Quote: ... 10% fetal calf serum (FCS; Gibco by Thermo Fischer Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... supplemented with 10 % FCS (FBS, Gibco), 1% pen/strep (Merck) ...
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bioRxiv - Molecular Biology 2019Quote: ... supplemented with 10% FCS (Life Technologies). LOVO ...
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bioRxiv - Molecular Biology 2019Quote: ... supplemented with 10% FCS (Life Technologies) and 1 x Penicillin-Streptomycin (15070063 ...
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bioRxiv - Developmental Biology 2020Quote: ... containing 10% FCS (Thermo Fisher Scientific) and 1% Glutamine-Penicillin-Streptomycin solution (Sigma-Aldrich) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... supplemented with 10% FCS (#10500064, Gibco) and penicillin-streptomycin (#15070063 ...
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bioRxiv - Neuroscience 2019Quote: ... supplemented with 10% FCS (10082139, Invitrogen), and penicillin (100 UI/mL)-streptomycin (100 μg/mL ...
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bioRxiv - Molecular Biology 2020Quote: ... 10% FCS (Fetal Calf Serum, Invitrogen) 0.5mg/mL DNase (Promega ...
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bioRxiv - Neuroscience 2020Quote: ... supplemented with 10% FCS (Fisher Scientific), 100 units/ml of Penicillin/Streptomycin (Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 10% FCS (Thermo Scientific), 1 mM L-glutamine (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 10% FCS (Cellgro Gibco), 1% PEN/STREP (Cellgro Gibco) ...