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Citations for Thermo Fisher :
1551 - 1600 of 10000+ citations for Recombinant Human TNFRSF18 Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... S1-RBD and ACE2 recombinant proteins were expressed in Expi293 cells for 5 days following transient transfection using Expifectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell cultures were centrifuged at 4500g for 40 min ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.4 picomol of folded reporter mRNA was mixed with 20 picomol of recombinant protein and 200 picomol of UltraPure BSA (Thermo Fisher # AM2618) in binding buffer (10 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... 28 fmol of folded reporter mRNA was mixed with 0-10 picomol of recombinant protein and 100 picomol of UltraPure BSA (Thermo Fisher # AM2618) on ice for 1 hr ...
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bioRxiv - Microbiology 2023Quote: Concentration of recombinant proteins were determined by measuring absorbance at 280 nm using a NanoDrop 1000 and/or NanoDrop One (Thermo Fisher Scientific) considering the extinction coefficient and the molecular weight calculated with the ProtParam tool (http://www.expasy.org/tools/protparam.html).
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bioRxiv - Cancer Biology 2023Quote: ... Single guide RNAs (sgRNAs) were incubated together with recombinant Cas9 (TrueCut Cas9 Protein v2, 5ug/ul, Thermo Fisher Scientific, cat. no. A36499) for 15 min at room temperature ...
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bioRxiv - Genomics 2023Quote: ... Integrity of the GST tag was subsequently checked via western blotting of the purified recombinant protein using anti-GST antibodies (Invitrogen 71-7500) [1:1,500 dilution] ...
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bioRxiv - Cell Biology 2023Quote: ... and Alt-R® CRISPR-Cas9 tracrRNA Atto550 labeled, IDT) and recombinant Cas9 protein (Alt-R® S. p. HiFi Cas9 Nuclease V3, IDT) using Lipofectamine™ CRISPRMAX (Invitrogen) and cultured for 48 h in the presence of an HDR enhancer (final concentration 20 μM ...
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bioRxiv - Microbiology 2023Quote: ... AcGFP-JEVcore recombinant protein and Alexa Fluor 594 or 555-conjugated antibody (2 mg/mL, Thermo Fisher Scientific, as an injection marker) were microinjected into the cytoplasm at a final concentration of 1 mg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... Expression of the recombinant LZTR1 proteins was confirmed by Western blot analysis using an anti-His tag monoclonal rabbit antibody (Thermo Fisher Scientific). Following confirmation of expression ...
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bioRxiv - Biochemistry 2023Quote: ... Phage pools representing the libraries were cycled through rounds of binding selections with EPHA2-FN2-Fc fusion protein immobilised on Maxisorp Immuno plates (ThermoFisher, #12-565-135), as described previously [21] ...
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bioRxiv - Synthetic Biology 2022Quote: ... while the perfluorinated ether FC-770 and the perfluorinated amine FC-3283 were obtained from Acros Organics (Geel, Belgium). Ethanol (96% vol. ...
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bioRxiv - Neuroscience 2020Quote: ... sections were rinsed in 0.1 M PBS and incubated in Alexa Fluor 647-tagged goat anti-mouse IgG or Alexa Fluor 750-tagged goat anti-rabbit IgG (1:100, Thermo Fisher, cat# A21235 and A21039), or Alexa Fluor 647-tagged donkey anti-mouse IgG ...
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bioRxiv - Microbiology 2019Quote: ... Raw cells were transfected with 7ug reporter RNAs either with or without 30 pmoles of biotinylated miR-142-3p mimic or scrambled 3’ biotinylated mimic (Dharmacon) using the Neon Transfection System (Invitrogen; 1750V, 25ms, 1 pulse). At 1.5-2h post-transfection ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 antigens were biotinylated using EDC (Thermo Fisher) and Sulfo-NHS-LCLC biotin (Thermo Fisher ...
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bioRxiv - Biochemistry 2021Quote: ... All targets were chemically biotinylated using NHS-Biotin (ThermoFisher #20217) according to the manufacturer protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... biotinylated junctions were isolated with streptavidin beads (65601, Life Technologies); and DNA libraries were prepared ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated antigens were coupled to FluoSphere NeutrAvidin beads (Thermo Fisher) and incubated with 10 μl 1:100 diluted plasma for 2h at 37°C to form immune-complexes ...
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bioRxiv - Immunology 2022Quote: ... biotinylated antigens were coupled to FluoSphere NeutrAvidin beads (Thermo Fisher) and incubated with 10 μl 1:100 diluted plasma for 2h at 37°C to form immune complexes ...
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bioRxiv - Immunology 2022Quote: ... biotinylated antigens were coupled to FluoSphere NeutrAvidin beads (Thermo Fisher) and to form immune-complexes incubated with 10 μl 1:10 diluted plasma samples for 2h at 37°C ...
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bioRxiv - Genomics 2021Quote: ... deoxynucleotides (A,G,C (sigma) and biotinylated-dUTP (ThermoFisher Scientific)) to a final concentration of 100 nM ...
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bioRxiv - Biochemistry 2020Quote: ... and biotinylated donkey anti-rabbit IgG (Cat.# A16027; Thermo Fisher).
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bioRxiv - Neuroscience 2020Quote: ... An anterograde tracer of 5% biotinylated dextran amine (BDA; Invitrogen) was used in some cases ...
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bioRxiv - Cell Biology 2021Quote: ... The biotinylated DNA was coupled to Dynabeads Kilobinders (Thermofisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... was biotinylated using biotin-NHS (Thermo Scientific, catalog number: 21343). Flow chambers for microscopy were assembled by sandwiching a piece of double-sided tape between a slide and a plasma cleaned coverslip (Fisherbrand ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 antigens were biotinylated using EDC (Thermo Fisher) and Sulfo-NHS-LCLC biotin (Thermo Fisher ...
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bioRxiv - Molecular Biology 2020Quote: ... All probes were ordered biotinylated at the 3’ end (Invitrogen).
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bioRxiv - Microbiology 2020Quote: ... Biotinylated ASOs were synthesized by RevertAid Reverse Transcriptase (Thermo Scientific) and 5’ biotin-TEG primer ...
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bioRxiv - Cell Biology 2020Quote: ... lectins were biotinylated by NHS-ester conjugation (Thermo Fisher Scientific) and dialyzed against DPBS−/− overnight ...
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bioRxiv - Neuroscience 2021Quote: Secondary antibodies: Biotinylated anti-rabbit (1:500, Invitrogen, Carlsbad, USA). Fluorescent anti-rat (Alexa 594 ...
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bioRxiv - Molecular Biology 2021Quote: ... and biotinylated at 3’ end using a biotinylation Kit (Thermofisher). H9 cell nuclear extracts (NEs ...
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bioRxiv - Immunology 2021Quote: ... Biotinylated Spike was expressed in 1L of HEK293F cells (Invitrogen) at a density of 1.5 × 106 cells ml-1 ...
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bioRxiv - Bioengineering 2021Quote: ... Biotinylated lotus tetranoglobus lectin was purchased from Fisher Scientific (#NC0370187) and visualized with streptavidin-Cy5 (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Biotinylated products were purified using M270 dynabeads (ThermoFisher, Waltham, MA) by incubating the reaction for 30 minutes at room temperature followed by magnetic separation of bound DNA ...
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bioRxiv - Cell Biology 2023Quote: ... and biotinylated by using maleimide-PEG2-biotin (Thermo Fisher Scientific). Mono-biotinylated Fab’ fragments of goat anti-mouse Ig(G+M ...
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bioRxiv - Developmental Biology 2023Quote: ... biotinylated amplicon is purified using MyONE C1 streptavidin beads (Thermofisher), removing unwanted DNA ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotinylated ligation products were pulled down using streptavidin beads (Invitrogen) followed by A-tailing and adaptor ligation for library construction ...
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bioRxiv - Biochemistry 2023Quote: ... were first incubated with Biotinylated Oligo (dT) probes (20mer, ThermoFisher) at 42°C for 30 min in hybridization buffer (10 mM Tris–HCl pH 7.5 ...
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bioRxiv - Immunology 2023Quote: ... was biotinylated with Sulfo-NHS-LC-LC Biotin (ThermoFisher, A35358). Excess biotin was removed from each with 3-kDa-cutoff centrifugal filter units (Amicon) ...
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bioRxiv - Cell Biology 2023Quote: ... the biotinylated probe was visualised using Avidin Cy5 (ThermoFisher Scientific). A minimum of 25 images per cell line were acquired and analysed with the Leica Cytovision software ...
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bioRxiv - Biophysics 2023Quote: ... we further incubate NP-streptavidin complex with biotinylated EGF (Thermofisher) or biotinylated transferrin (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... Biotinylated mAbs were prepared using Biotin-XX (Thermo Fisher Scientific). Biotinylated mouse anti-mouse TLR4 mAb (UT49 ...
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bioRxiv - Bioengineering 2023Quote: ... biotinylated goat anti-rabbit IgG (Cat.# 65-6140, Thermo Fisher), Alexa Fluor 488-conjugated goat anti-rabbit IgG (ab181448 ...
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bioRxiv - Immunology 2023Quote: ... to detect monomeric biotinylated antigens and anti-FITC antibody (Invitrogen) to detect Avidin-FITC conjugated tetrameric antigens ...
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bioRxiv - Cell Biology 2024Quote: ... two microliters of biotinylated anti-EpCAM (D20655, Thermo Fisher Scientific) and biotinylated CD56 (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: Uricase was biotinylated using EZ-link NHS-biotin (Thermo Scientific). Unreacted NHS-biotin was removed using Zeba spin desalting columns ...
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bioRxiv - Bioengineering 2024Quote: Rasburicase was biotinylated using EZ-link NHS-biotin (Thermo Scientific). Unreacted NHS-biotin was removed using Zeba spin desalting columns ...
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bioRxiv - Biophysics 2024Quote: ... Reduced antibodies were then biotinylated with Maleimide-PEG2Biotin (Thermo Scientific). Nonreacted DTT and unbound biotin were removed by overnight dialysis ...
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bioRxiv - Immunology 2019Quote: ... the wild-type and mutant (C302/305R) recombinant Mul1 proteins were produced in Sf9 cells using the Bac-to-Bac Baculovirus expression system (Invitrogen, Carlsbad, CA, USA) and purified by a nickel-chelating resin (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: In vitro binding experiments were performed with recombinant GST fusion proteins purified from E.coli and immobilized on glutathione-Sepharose 4B beads (Life Technologies #17-0756-01). Purification of GST fusion proteins was performed as described (Ebnet et al. ...
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bioRxiv - Neuroscience 2020Quote: ... The same recombinant Cdk5/p25 protein used for the MST assays was labeled with biotin using EZ-LinkNHS-PEG4-Biotinylation Kit (Thermo Scientific, Waltham, MA) and excess reagent was removed with a spin desalting column as per the manufacturer’s instruction ...