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Citations for Thermo Fisher :
1301 - 1350 of 1901 citations for PVRIG Cynomolgus HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: Cell surface proteins from transfected HEK293 cells were biotinylated using the Pierce Cell Surface Protein Isolation Kit (ThermoFisher Scientific., cat# 89881). Briefly ...
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bioRxiv - Immunology 2022Quote: ... All soluble SOSIP Envs were expressed by transient transfection in HEK293-6E cells (National Research Council of Canada) or Expi293 cells (Life Technologies) and purified from transfected cell supernatants by 2G12 affinity chromatography ...
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bioRxiv - Genetics 2023Quote: HEK293 cells were harvested and lysed in RIPA lysis with 1X Halt™ Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific). The concentration of total protein was determined using the BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... Constructs carrying the 3’UTRs were transiently co-transfected with vectors carrying Renilla-Luciferase and either miR-122 mimic (122-MIM) or scramble oligos (SCR) into HEK293 cells with Lipofectamine RNAiMAX (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... and human embryonic kidney 293 cells (HEK293) were purchased from ATCC and cultured in Dulbecco’s Modified Eagle Medium (DMEM) high glucose (Fisher Scientific, #MT10013CV) with 10% FBS at 37 °C and 5% CO2 ...
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bioRxiv - Molecular Biology 2023Quote: Approximately 4 × 106 HEK293 Flp-In T-Rex cells with EGFP integrants were seeded in 15-cm dishes (Thermo Fisher Scientific) and incubated overnight ...
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bioRxiv - Neuroscience 2023Quote: ... including Camuiα were transfected into HEK293S GnTI-cells (2 x 106 cells per well in 6-well plates) cultured in FreeStyle 293 (Thermo Fisher), using the TransIT2020 transfection reagent (Mirus Bio) ...
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bioRxiv - Developmental Biology 2023Quote: ... Culture inserts were removed 8h prior to imaging and HEK293 culture medium was changed to imaging medium – phenol red-free DMEM (Gibco, #31053028) with 10% FCS (Biosera ...
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bioRxiv - Cell Biology 2023Quote: ... Flp-In HEK293 cells were co-transfected with the pCDNA3-TurboID-cyclin F plasmid and the pOG44 Flp-Recombinase Expression Vector (Invitrogen, V600520) for co-expression of the Flp-recombinase using Lipofectamine 2000 transfection reagent (Invitrogen ...
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bioRxiv - Genetics 2024Quote: Flag-tagged wild type and mutated human RBBP5 plasmid were transfected to HEK293 cells by lipofectamine 3000 according to manufacturer’s protocol (Invitrogen, CA, USA). Cells were lysed by RIPA buffer and 20μg of whole cell lysate were used to assess protein expression in Western blot ...
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bioRxiv - Cancer Biology 2021Quote: ... A region spanning 2163 bps upstream from the transcription start site was PCR amplified using genomic DNA isolated from HEK293 cells with Platinum SuperFi II DNA polymerase (Thermo Fisher Scientific). The PCR product was cloned into pGL3 basic vector following conventional protocols ...
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bioRxiv - Cell Biology 2020Quote: Samples prepared following immunoprecipitation from HEK293 MceC3XFLAG cell line were analysed by LC-MS on an Orbitrap Elite™ mass spectrometer (ThermoFisher Scientific) coupled to a Dionex Ultimate 3000 Ultra-Performance Liquid Chromatography (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 Phoenix cells were transfected with the pBabe plasmid DNA encoding for the protein of interest using Lipofectamine 3000 (Thermo Fisher Scientific) per manufacturer’s recommendation ...
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bioRxiv - Molecular Biology 2019Quote: ... Transfection of Flp-In T-Rex HEK293 cells was carried out according to Flp-In™ T-REx™ Core Kit (Invitrogen). Briefly ...
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bioRxiv - Cell Biology 2020Quote: HEK293 cells were transfected with pcDNA3.1-GAS6-MycHis linearized with PvuI restriction enzyme using Lipofectamine® 2000 Transfection Reagent (Thermo Fisher Scientific), according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... The P2 baculovirus produced in Sf9 cells was added to HEK293 GnTI- cells (mycoplasma test negative, ATCC #CRL-3022) and grown in suspension in FreeStyle medium (GIBCO-Life Technologies) supplemented with 2% FBS at 37°C and 8% CO2 ...
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bioRxiv - Biochemistry 2020Quote: pcDNA5-based constructs for the expression of C-terminally His6-PreScission protease cleavage site-2xFlag (Flag)-tagged full-length or N-terminally truncated ASCC3 variants were transfected into HEK293 Flp-In™ T-REx™ cells (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... For the preparation of the BGLF2-KO virus, HEK293/EBV(dBGLF2) cells (Konishi et al., 2018) were transfected with BZLF1 expression plasmid using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Linearized plasmids containing the ItypOrco gene were transfected into HEK293 cells containing a tetracycline-inducible repressor (TREx) using Lipofectamine 2000 (Thermo Fisher Scientific) (Corcoran et al ...
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bioRxiv - Microbiology 2020Quote: ... HEK293-EBV B(+/−) S13+/− cells were transfected with pCDNA3-BZLF1 and pCDNA3-BALF4 using TurboFect Transfection Reagent (Thermo Scientific, Waltham, MA, USA). Old medium was changed with fresh medium in one day post transfection ...
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bioRxiv - Immunology 2021Quote: HEK293 adherent cells grown to a confluency of 70–90% were transfected using a Lipofectamine 3000 Reagent Kit (L3000075, Invitrogen, Waltham, MA). Transfection procedures were adapted from the product manual ...
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bioRxiv - Microbiology 2022Quote: ... Titration of progeny viruses from all HEK293 cells was done in Vero-E6 cells cultured in 16-well chambered slides (Thermo Fisher Scientific), as described below ...
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bioRxiv - Genetics 2022Quote: ... These plasmids were inserted into the same place in the genome using the Flp-In recombinase technology to generate Flp-In HEK293 (Thermofisher, cat. #R78007) stable cell lines ...
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bioRxiv - Molecular Biology 2021Quote: HeLa cells and HEK293 cells (in 35-mm dishes) were transfected with 100 pmol siRNA using Lipofectamine RNAi max (Invitrogen–Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: HEK293 cells were grown in a monolayer on 10 cm culture dishes in Dulbecco’s Modified Eagle Medium (DMEM) (ThermoFisher Scientific, Waltham, MA) supplemented with fetal bovine serum (10% v/v ...
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bioRxiv - Molecular Biology 2019Quote: ... Virus-like particles (VLPs) containing either dCas9-KRAB or a targeting sgRNA were generated by transfection of HEK293 cells with Lipofectamine 2000 (Thermo Fisher Scientific). Cells were cotransfected with the packaging plasmid pCMV-dR8.91 ...
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bioRxiv - Systems Biology 2021Quote: HEK293 cells were maintained at 37°C and 5% CO2 in phenol red-free Dulbecco’s Modified Eagle Medium (Gibco, Thermo Fisher Scientific) supplemented with 10% fetal calf serum (Sigma-Aldrich (MilliporSigma)) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... HEK293 cells were a gift from Dr Harvey McMahon and were cultured in high-glucose DMEM supplemented with GlutaMAX (Thermo Fisher, 31966047) and 10% FBS (Thermo Fisher ...
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bioRxiv - Cancer Biology 2020Quote: Cell lines were made by co-transfecting plasmids with lentiviral packaging vectors (VSVG and SPAX2) into packaging cells (HEK293-FT) using Lipofectamine 2000 (Thermo Fisher Scientific). Viral supernatants were collected ...
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bioRxiv - Biophysics 2021Quote: The HEK293 cell line subclone TS201A was a gift from Vladimir Yarov-Yarovoy and was maintained in DMEM (Gibco Cat# 11995-065) with 10% Fetal Bovine Serum (HyClone ...
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bioRxiv - Neuroscience 2022Quote: HEK293-T cells of low passage (<25) were kept in Dulbecco′s Modified Eagle′s Medium (DMEM, Thermo Fisher Scientific #31966-047) containing 10% fetal calf serum (FCS ...
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bioRxiv - Biochemistry 2022Quote: ... 12 pmol crRNA:tracrRNA duplex was combined with 12 pmol recombinant HiFi Cas9 (IDT) and reverse transfected into HEK293 Flp-In cells using Lipofectamine CRISPRMAX reagent (Thermo Fisher Scientific) following IDT protocols ...
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bioRxiv - Genetics 2022Quote: ... pgLAP1-3MYC-BioID2 subnit plasmids were then transformed and stably integrated into HEK293-Flp-In-T-REx cells using the manufacturer’s protocol (ThermoFisher Scientific, catalogue #R78007).
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bioRxiv - Cell Biology 2024Quote: Measurement of extracellular Ca2+ clearance by mitochondria from digitonin-permeabilized HEK293 cells was performed using the membrane impermeable Ca2+ indicator Calcium-Green-5N (Life technologies, C3737). Cells were harvested at a density of 500000 cells/mL in growth medium supplemented with 20 mM HEPES (pH 7.2/NaOH) ...
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bioRxiv - Cell Biology 2023Quote: ... pLVX-DsRed-Spartin plasmid together with psPAX2 and VSV-G plasmid were transfected into HEK293 cells using Lipofectamine 3000 (L3000015; Thermo Fisher Scientific) for generating lentivirus ...
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bioRxiv - Cell Biology 2022Quote: ... into the Flp-In™ T-REx™ HEK293 cell line or Jaw1 KO cells (#R78007; Thermo Fisher Scientific, Waltham, MA, USA). After incubation for 24 h ...
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bioRxiv - Molecular Biology 2023Quote: ... The mCherry tagged SETD6 WT or Y285A and EGFP-tagged E2F1 WT or K117R were co-transfected into HEK293 cells using polyethyleneimine (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Expression vectors of SLC22A10 orthologs were introduced into HEK293 Flp-In cells either through transient transfection or stable transfection using Lipofectamine LTX (Thermo Fisher Scientific). For transfections in a 48-well plate (seeding density ...
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bioRxiv - Physiology 2023Quote: ... mVenus:FL_ Proline and mVenus:2x linker proteins were transfected into HEK293 (Catalog CRL-1573, ATCC) cells with lipofectamine 3000 transfection reagent (Catalog L3000008, Thermo Fisher Scientific). 24h post-transfection ...
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bioRxiv - Biophysics 2023Quote: S1PR1-Flag and CD69-StrepII were separately expressed using baculovirus-mediated transduction of mammalian HEK293S GnTI− cells (ATCC CRL-3022) in a medium containing FreeStyle 293 (Gibco Cat# 12338018) supplemented with 2% charcoal-dextran stripped fetal bovine serum (Gibco Cat# 12676029) ...
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bioRxiv - Genetics 2023Quote: ... Lentiviruses were produced transfecting HEK293 cells growing on a T75 flask with 20ug of total DNA and Lipofectamine 2000 (Thermo Fisher Scientific) for 16h ...
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: The plasmids coding for pro-BMP9 and pro-BMP10 were co-transected in equal amounts into HEK293 cells (expi293, Invitrogen/Thermo Fisher Scientific) and after five days ...
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bioRxiv - Molecular Biology 2024Quote: The transfection of HeLa and HEK293 cells with siRNAs (100 pmol each) was performed using Lipofectamine RNAi max (Invitrogen-Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: The transfection of HeLa and HEK293 cells with siRNAs (100 pmol each) was performed using Lipofectamine RNAi max (Invitrogen-Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... was added for some experiments. HEK293 cells (a gift from H. Choe) used for generation of lentiviral particles were maintained in DMEM (Gibco #11995-065) supplemented with 10% FBS and antibiotic–antimycotic reagent (Gibco #15240-062 ...
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bioRxiv - Biochemistry 2024Quote: ... Expi293F cells (derived from HEK293 cells) were grown in suspension and shaken at 125 rpm using serum-free Expi293TM expression medium (Gibco, Cat#A1435101) according to the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2021Quote: ... Sybr Safe DNA gel stain and BenchMark™ His-tagged Protein Standard were purchased from Invitrogen. HRP-conjugated 6*His His-Tag Mouse McAB was obtained from Proteintech ...
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bioRxiv - Evolutionary Biology 2020Quote: ... His-tagged proteins were eluted from the beads in a disposable polypropylene column (29924, ThermoFisher Scientific) using elution buffer (4 ml wash buffer containing 400 mM imidazole ...
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bioRxiv - Microbiology 2019Quote: ... lipid membranes were incubated with a 6x-His tag polyclonal antibody HRP conjugate (MA1-21315, ThermoFisher) in blocking buffer for one hour at room temperature followed by three washes with wash buffer ...
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bioRxiv - Neuroscience 2021Quote: ... Mouse SGK1.1 (wild type and constitutively active mutant S515D) cloned into pcDNA3.1/V5-His-TOPO (Invitrogen) were kind gifts from Dr ...