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Citations for Thermo Fisher :
1301 - 1350 of 10000+ citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Serial coronal frozen sections (25 μm thickness) were cut with a freezing microtome (Microm HM 450, ThermoFisher), collected in a 24-well plate ...
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bioRxiv - Cell Biology 2024Quote: Frozen brain samples were coronally sectioned at 50 μm thickness on a sliding microtome (Thermo Scientific #HM430) at -20 °C ...
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bioRxiv - Cell Biology 2020Quote: ... transfected cells were treated with 100 µM oleic acid and 0.5 µM BODIPY Red (Invitrogen) for 24 hrs ...
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bioRxiv - Biophysics 2024Quote: ... Lyso-Laurdan (2.5 µM) and Mito-Laurdan (2.5 µM) for 20 minutes in FluorobriteTM (Gibco). All OTLs were dissolved in DMSO before being diluted to their respective concentrations in Fluorobrite ...
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bioRxiv - Immunology 2024Quote: ... 1 µM or 0.4 µM of 5(6)-carboxyfluorescein diacetate succinimidyl ester (CFDA-SE; Invitrogen), which is metabolized within cells to carboxyfluorescein succinimidyl ester (CFSE) ...
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bioRxiv - Bioengineering 2021Quote: For sender membrane preparation the nitrocellulose membranes (ThermoFisher Scientific, USA, 88018) were cut into squares (8 × 8 mm ...
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bioRxiv - Neuroscience 2020Quote: Neuronal membrane potential was measured using Fluovolt Membrane Potential Kit (ThermoFisher) as per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... the membrane was stained with Novex reversible membrane protein stain (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2020Quote: Cas9 was produced by IVT using either a continuous exchange 1-Step Human Coupled IVT Kit (Thermo Fisher Scientific) or a PURExpress bacterial IVT kit (New England Biolabs) ...
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bioRxiv - Microbiology 2021Quote: ... diluted 2 times with 0.1 % formic acid and desalted on strong cation exchange micro-tips (StageTips, Thermo Fisher scientific) as described72 ...
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bioRxiv - Neuroscience 2022Quote: Sample temperature was controlled by heat exchange with one of two circulating water baths (Accel 250 LC, Thermo Scientific, USA ...
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bioRxiv - Immunology 2023Quote: ... and buffer exchange was performed with a 7 kDa molecular weight cut-off size exclusion resin (Thermo Fisher Scientific) to cryo-EM buffer (10 mM Tris at pH 7.6 and 20 mM NaCl).
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bioRxiv - Neuroscience 2023Quote: ... at 5,000g and 4°C following buffer-exchange in PBS (pH 7.4) with the inclusion of non-ionic surfactant Pluronic F-68 (cat#. 24040032, Gibco) (final storage solution ...
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bioRxiv - Microbiology 2024Quote: ... subjected to buffer exchange with phosphate buffered saline (PBS) pH 7.4 (Fisher Bioreagents, Thermo Fisher Scientific, Waltham, MA, USA) and concentrated as above described ...
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bioRxiv - Immunology 2024Quote: Cation exchange chromatography was conducted using an LC-2030C chromatograph with a MAbPac SCX column (Thermo Scientific, Whaltham, MA). Mobile phase A comprised 20 mM 2-(N-morpholino ...
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bioRxiv - Biochemistry 2024Quote: ... the cells were subjected to antibiotic selection by exchange into adherent culture media containing 2 μg/mL blasticidin (Gibco) for 10 days with regular exchange into fresh selective media and passaging to prevent cells from achieving full confluency ...
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bioRxiv - Biochemistry 2024Quote: Cleaved protein was further purified by ion exchange chromatography on a 5 mL HiTrap Q column (Thermo Fisher Scientific) with elution over a linear gradient from 100% buffer A (25 mM Tris pH=7.5 ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were treated with 10 µM EdU using a Click-iT EdU Alexa Fluor 488 flow Cytometry Assay Kit (Thermo Fisher, C10425) and processed as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Cas9-gRNA RNP was formed by mixing 10 µM of the annealed tracrRNA-crRNA and 16.5 µg of TrueCut Cas9 protein (Thermo Fisher Scientific, A36498) in PBS and incubating at room temperature for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were collected by trypsinization and resuspended in FACS buffer (10% FBS, PBS, ± 500 µM auxin) with SYTOX Blue (Thermo Fisher Scientific) for sorting ...
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bioRxiv - Genetics 2020Quote: ... Blocks were later cut on a cryostat and 7-10 µm thick sections were placed on SuperFrost Plus Gold slides (ThermoFisher; Cat# FT4981GLPLUS). Hematoxylin and eosin (H&E ...
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bioRxiv - Neuroscience 2021Quote: ... Culture medium with SHH (100 ng/ml) and DAPT (10 µM) were changed daily and shifted from mTeSR1 to N2 (Thermo Fisher Scientific) in 3 days ...
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bioRxiv - Cell Biology 2021Quote: ... the synchronized animals (24 h after L4 molt) were soaked in 50 µl MitoTracker Red CMXRos (Invitrogen, 10 µM in M9 buffer) for 10 min at 20°C in the dark ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated with 10 µM EdU for 3 h and analyzed using Click-iTTM EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Thermo Fisher Scientific). Cells were recorded with a BDTM LSRII (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated with EdU (10 µM) for 2h followed by fixation and Click-it reaction according to instructions for flow cytometry (Life Technologies, C10424) or by secondary antibody incubation for confocal analysis (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... compounds were added to the internal solution to visualize cell morphology or confirm cell identity and location: Alexa Fluor 488 (10-20 µM, Thermo Fisher Scientific), Neurobiotin (0.1% ...
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bioRxiv - Bioengineering 2021Quote: ... These were then treated with 10 µM Ethidium Homodimer-1 and 5 uM Calcein AM from a Live/Dead Test Kit (Molecular Probes, Thermofisher Scientific) to a total volume of 250 uL PBS and incubated (37°C ...
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bioRxiv - Cell Biology 2020Quote: ... organoids (after 1 week of culture) were incubated (37°C, 5% CO2, 1 h) with 10 µM EdU (5-ethynyl-2’-deoxyuridine, Thermo Fisher Scientific), a nucleoside analog of thymidine incorporated into DNA during active DNA synthesis ...
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bioRxiv - Genomics 2021Quote: ... Approximately 5 × 106 cells at a density of 1 × 106 cells/mL were plated in 60mm cell culture dishes with 10 µM of PMA (Thermo Fisher Scientific). Media was changed every 48 hours and 10µM of PMA was added at every media change ...
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bioRxiv - Neuroscience 2022Quote: ... isolated brainstem preparations with the pia carefully removed were first incubated in the dark for 45 min at room temperature in a solution of oxygenated aCSF containing the cell-permeable calcium indicator dye Calcium Green-1 AM (10 µM; Life Technologies, France). After dye-loading ...
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bioRxiv - Neuroscience 2022Quote: ... Measurements were performed on permeabilized fibers in Buffer Z media at 37 ∼C containing: 10 µM Amplex UltraRed (Molecular Probes, Eugene, OR), 1 U/mL horseradish peroxidase (HRP) ...
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bioRxiv - Microbiology 2024Quote: ... experiments were standardized as follows: 10 µM of the protein of purified CoaX was mixed with 5 µL 1X SYPRO Orange (Thermo Fisher Scientific) and various concentrations of substrates (1-10 mM ATPγ or 0-5 mM pantothenate ...
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bioRxiv - Microbiology 2024Quote: ... the coverslips were inverted onto a 30- µl droplet of freshly prepared CuAAC click reaction mix [10 µM N3-AlexaFluor647 (Thermo Fisher Scientific), 1 mM CuSO4 ...
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bioRxiv - Genomics 2024Quote: Probes were hybridized to the sample at a concentration of 6-10 µM in a 30% v/v formamide (Fisher Scientific, AM9342), 10% w/v dextran sulfate (VWR ...
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bioRxiv - Microbiology 2024Quote: ... human skin samples were embedded in OCT matrix (Tissutek, Saktura) and 5 or 10 µm sections were sliced with an CryoStar™ NX70 cryostat (Thermo Scientific) and collected on SuperFrost® Plus microscopy slides (Thermo Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... 200 µL of culture was removed and cell density was normalized before staining with carboxy-H2DCFDA fluorescent dye (final concentration 10 µM) (Invitrogen, Waltham, MA). Samples were incubated at room temperature for 5 minutes ...
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bioRxiv - Genetics 2024Quote: 10 µm axial or longitudinal cryostat sections were placed on Fisherbrand™ ColorFrost™ Plus slides (Fisher Scientific, catalog # 12-550-18) and air dried for 12-15 hours overnight in the dark at RT ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... to obtain 30 μm tissue sections86 that were then stained for 10 min with 0.3 µM DAPI in DPBS (Thermo Scientific™ #62247). The sections were mounted with Fluoromount-G™ mounting medium (Invitrogen #00-4958-02 ...
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bioRxiv - Physiology 2023Quote: ... Cells were then spun at 350 x g for 4 min and resuspended in 1 mL FSW with 10 µM pH-sensitive cell dye SNARF-1 acetoxymethyl ester acetate (Invitrogen, Thermo Fisher Scientific) and 0.1% dimethylsulfoxide (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: Knockdown of PR130 in murine and human PDAC cells was performed by transfecting 30 pmol of validated siRNA molecules targeting the PPP2R3A/Ppp2r3a mRNAs (murine: Santa Cruz, sc-108917, 10 µM stock solution; human: Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... For co-localization experiments cells were also incubated together with cell-permeant dyes (50 nM LysoTrackerGreen, Thermo Fisher Scientific, L7526; 10 µM calcein, AM, Thermo Fisher Scientific ...
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bioRxiv - Pathology 2024Quote: ... 3 µL of diluted cDNA diluted was added to 1 µL of each primer (S1 Table) with 10 µM and 5 µL of SYBR Green Master Mix (Applied Biosystems, Life Technologies), using the following thermal program ...
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bioRxiv - Microbiology 2024Quote: ... HPLC was performed with a column temperature of 55°C using a linear 135-min gradient from 100% buffer A (10 mM sodium phosphate pH 6.0 and 20 µM NaN3) to 100% buffer B (10 mM sodium phosphate pH 6.0 and 30% methanol) (Fisher Scientific, Loμghboroμgh, UK). Muropeptide fractions were detected at 205 nm ...
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bioRxiv - Molecular Biology 2024Quote: ... Hatched larvae (n = ∼500 – 1,000) were subjected to three conditions: incubation with 10 µM ivermectin (IVM, Thermo Fisher Scientific, Ward Hill, MA), 10 µM moxidectin (MOX ...
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bioRxiv - Genomics 2024Quote: ... Labeling was performed for 10 minutes in 15 mL of media supplemented with 500 µM 4-Thiouridine (4-SU) (Thermo Fisher J60679) with GSI and/or BRM014 depending on the experimental condition ...
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bioRxiv - Neuroscience 2024Quote: ... cells were incubated in 2 µM of Fluo-3 or 10 µM of Mag-Fluo-4 for 30 mins in Hanks’ Balanced Salt Solution (HBSS) (catalog number 14025092, Thermo Fisher Scientific). Cells were then washed two times with HBSS and kept in HBSS for de-esterification for an additional 15 mins before imaging.
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bioRxiv - Immunology 2024Quote: ... 1 mm3 burns were made with a cauterizer and the injury site was then stained with 10 µl of pHrodo Red succinimidyl ester (SE) (4 µM; Thermo Fisher Scientific). The incision was stitched ...
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bioRxiv - Biophysics 2024Quote: ... cells were removed from the incubation and treated with 10 µM compounds in serum-free CO2 independent media (Gibco L15 media, # 2108302) for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% [v/v] Anti-Anti [Gibco Cat#15240062]) with 10 µM Y-27632 and 4ng/mL thermostable human basic fibroblast growth factor (bFGF, Gibco Cat#PHG0360). The fifth day ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were exposed to the medium supplemented with 100 nM HaloTag TMR ligand and 10 µM 5-ethynyl-2′-deoxyuridine (EdU) (C10337; Thermo Fisher Scientific) for 30 min immediately after washing out 7BRO ...