Labshake search
Citations for Thermo Fisher :
1251 - 1300 of 10000+ citations for Zika Virus NS1 Protein Uganda strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... viral stocks were prepared after two serial passages of isolated virus in Vero E6 cells in Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) supplemented with 5% fetal bovine serum (Thermo Fisher Scientific) ...
-
bioRxiv - Immunology 2020Quote: ... Ebola virus Makona Glycoprotein (EBOV-GP) (KM233102.1) was synthesized and cloned into pcDNA3.1 by GeneArt AG technology (Life Technologies, Regensburg, Germany) or VSV-G following the manufacturer’s instructions of Lipofectamine 3000 (Fisher Scientific) ...
-
bioRxiv - Microbiology 2021Quote: ... The eluted RNA was combined with Applied Biosystems™ TaqMan™ Fast Virus 1-step master mix (ThermoFisher, Waltham, MA, USA). The target regions for the CSG ...
-
bioRxiv - Microbiology 2021Quote: ... 5 μl of the RNA extraction final elution was reverse-transcribed to cDNA and amplified according to the manufacturer’s protocol using TaqMan™ Fast Virus 1-Step Master Mix (ThermoFisher Scientific). The primer pair was as follows ...
-
bioRxiv - Immunology 2020Quote: ... Virus containing supernatants were harvested 2 days later and the produced infectious virus yield was measured using TZM-bl reporter cell line and Gal-Screen kit from Applied Biosystems as recommended by the manufacturer ...
-
bioRxiv - Cell Biology 2021Quote: ... Induced pluripotent stem cells were derived from human fibroblasts using Sendai virus-based reprogramming kit (CytoTune-iPS Sendai Reprogramming Kit; cat #. A13780-01; Thermo Fisher), according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... and number of virus-infected cells (based on dsRNA and nucleocapsid staining) was performed using the CellInsight CX7 HCS platform (Thermo Fisher), as previously described 55,56 ...
-
bioRxiv - Microbiology 2021Quote: ... HEK293T cells were co-transfected with the pNL4.3.Luc.R-E-viral vector as well as a plasmid expressing the vesicular stomatitis virus glycoprotein (VSV-G) envelope (pMD2.G) using Lipofectamine 3000 (Life Technologies) according to basic manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2022Quote: ... The supernatant containing the virus was then used fresh or stored by snap-freezing aliquots supplemented with 8 μg/ml polybrene (Fisher Scientific) in ethanol/dry ice and stored at -80° C ...
-
bioRxiv - Cell Biology 2022Quote: ... Transduction efficiency was assessed by GFP expressing virus and subsequent quantification of GFP-positive cells by Countess II FL Cell Counter (Thermo Fisher).
-
bioRxiv - Microbiology 2022Quote: ... RNA was extracted from 50 μl of virus inoculum suspension using the PureLink Viral RNA/DNA Mini kit (Themo Fisher Scientific), as per manufacturer’s directions.
-
bioRxiv - Microbiology 2022Quote: ... and resuspended in thawed Geltrex lactose dehydrogenase elevating virus free (LDEV-free) reduced growth factor basement membrane matrix (Thermo Fisher Scientific). Three 10-μl drops of the matrix-cell suspension were pipetted into wells of a Nunclon Delta 24-well plate (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... 7 µl total RNA was amplified in a mix containing 5 µl of TaqMan Fast Virus 1-step master mix (Life Technologies), 0.4 µl primer-probe mix for IAV 40 and 9.6 µl distilled water ...
-
bioRxiv - Microbiology 2022Quote: Copies of the SARS-CoV-2 N gene (genomic) were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Applied Biosystems). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
-
bioRxiv - Microbiology 2022Quote: Copies of SARS-CoV-2 E gene (subgenomic) were measured by qRT-PCR with TaqMan Fast Virus 1-step assay (Thermo Fisher). SARS-CoV-2 specific primers and probes sgLeadSARSCoV2-F 5’-CGATCTCTTGTAGATCTGTTCTC-3’ ...
-
bioRxiv - Microbiology 2022Quote: ... The virus-containing samples (50 μl) were serially diluted in 450 μl aliquots of serum-free Opti-Pro medium (Gibco, UK), after which 200 μl from each dilution were added in duplicates to the cells ...
-
bioRxiv - Microbiology 2022Quote: ... 5 µl total RNA was amplified in a mix containing 5 µl of TaqMan Fast Virus 1-step master mix (Life Technologies), 1 µl primer-probe mix for β-actin ...
-
bioRxiv - Neuroscience 2022Quote: ... HT-1080 cells (ATCC CCL-121; RRID:CVCL_0317, male) used for virus titration were grown and maintained in MEM (Gibco Cat#11095-080) supplemented with 10% FBS ...
-
bioRxiv - Microbiology 2023Quote: ... The samples were used for quantitating HuNoV RNA GEs by means of reverse transcription-quantitative PCR (RT-qPCR) analysis using a TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher) and GII specific primer/probe sets ...
-
bioRxiv - Cancer Biology 2024Quote: ... Subsequent qPCR was performed using the TaqMan Fast Virus 1-Step Master Mix and StepOnePlus Real-Time PCR System (Thermo Fisher). The primers purchased from TaqMan were ...
-
bioRxiv - Neuroscience 2024Quote: ... HEK cells below passage 10 are plated into a 15 cm dish/virus (Fisher 08-772-24) in DMEM-H (DMEM-h (invitrogen 10569010) + NEAA (Invitrogen 11140050) + 10% FBS (invitrogen 16140071 ...
-
bioRxiv - Genetics 2024Quote: The derivation of iPSCs was carried out by the NHLBI iPSCs core facility using the Cytotune 2.0 Sendai virus kit (ThermoFisher Scientific, A16517) with a few modifications for high throughput reprogramming essentially as described earlier (19) ...
-
bioRxiv - Microbiology 2024Quote: ... Virus stocks were grown in M2-10B4 cells (ATCC # CRL-1972) with RPMI 1640 Medium special formulation (Thermo Fisher cat # A1049101) supplemented with 10% fetal bovine serum (Thermo Fisher cat # 12483020 ...
-
bioRxiv - Biochemistry 2023Quote: ... the elutes of GlnR147 (his-TEV GlnR C60SL147C) from Ni-NTA column were cleaved with recombinant tobacco-etch virus protease (Life Technologies) overnight at 4 °C ...
-
bioRxiv - Microbiology 2023Quote: ... Anesthetized mice were inoculated intranasally with 30 μL of virus diluted in chilled 1X PBS (MHV) or administered undiluted in 2% FBS (Gibco, MA)/1X non-essential amino acids (Gibco ...
-
bioRxiv - Immunology 2023Quote: ... Both assays were performed using the Taqman Fast Virus 1-Step Master Mix RT-qPCR kit (Applied Biosystems Inc., Carlsbad, CA) on an LC96 instrument (Roche ...
-
bioRxiv - Cell Biology 2023Quote: The adeno-associated virus MyoAAV.2A was produced using the AAV-MAX Helper-Free AAV Production System Kit (Thermo Fisher Scientific). At 72 h post-transfection ...
-
bioRxiv - Biochemistry 2023Quote: ... Sf9 cells were infected (multiplicity of infection of 6 for each virus) with separate baculoviruses (prepared using the Bac-to-Bac Baculovirus Expression System, Invitrogen) containing the CXCL12LRHQ gene as well as the ACKR3 gene (residues 2-362 ...
-
bioRxiv - Immunology 2023Quote: ... 2μg liver RNA per sample was used for qRT-PCR analysis for Plasmodium 18S rRNA using TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems). Data were normalized for input to the GAPDH control for each sample and are presented as ratios of Plasmodium 18s rRNA to GAPDH RNA signals ...
-
bioRxiv - Microbiology 2023Quote: We diluted freshly purified virus samples in DPBS and combined three volumes of the virus with one volume of NuPAGE LDS Sample Buffer (4×; Thermo Fisher) containing 100 mM dithiothreitol ...
-
bioRxiv - Microbiology 2023Quote: ... For intravenous inoculation mice were restrained and injected via tail vein injection with 100 to 200 μl of virus or control inoculum using a 29G insulin syringe (Fisher Scientific). For natural transmission ...
-
bioRxiv - Pathology 2023Quote: ... was reverse transcribed at 37°C for 50 min in a 20 µl reaction mix containing 200U Moloney murine leukemia virus (MMLV) reverse transcriptase (Life Technologies), 100 ng random primers ...
-
bioRxiv - Genomics 2023Quote: ... the fibroblasts were thawed and plated at a density of 250K cells/well of 6-well plate and infected with the Cytotune Sendai virus (Life Technologies) per manufacturer’s protocol to initiate reprogramming ...
-
bioRxiv - Biochemistry 2023Quote: ... Baculoviruses were amplified twice and freshly generated P3 virus was used to infect 1.8-2 L of High Five cells (Thermo Fisher Scientific) at a density of 3-3.5 x106 cells/mL by adding 6 mL virus per 600 mL of cells ...
-
bioRxiv - Microbiology 2023Quote: ... Viral genome levels were quantified by qPCR using virus specific primer probe sets (described above) and normalised to GAPDH using an Applied Biosystems FAM/MGB probe mix (Thermofisher, 4333764F).
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Patients consent was retrieved and the iPSCs were generated from peripheral blood mononuclear cells (PBMCs) using Sendai virus (CytoTuneTM-iPS 2.0 Sendai Reprogramming Kit, Thermo Fisher Scientific) as previously described ...
-
bioRxiv - Neuroscience 2024Quote: ... The MS19-ES-H line was reprogrammed from PBMCs from a healthy female donor with a Cytotune Sendai Virus kit (Thermo Fisher).
-
bioRxiv - Microbiology 2024Quote: ... isolated RNA was run using a TaqMan Fast Virus One-Step Master Mix (applied Biosystems) on a QuantStudio 3 Flex Real-Time PCR system (Applied Biosystems). RNA standards with known copy numbers were used to generate a standard curve and calculate sample copy numbers ...
-
bioRxiv - Cancer Biology 2024Quote: ... Virus to construct stable HA-B56α overexpression cell lines were produced using HEK293T cells transfected using Lipofectamine 3000 (Fisher Scientific, L3000015) with the plasmid of interest (POI ...
-
bioRxiv - Genetics 2021Quote: ... Cas9 protein (Invitrogen), a single-stranded 100-bp oligo donor nucleotide (ssODN ...
-
bioRxiv - Cancer Biology 2024Quote: ... Protein gel (Invitrogen). Antibodies were added according to the manufacturers recommended conditions ...
-
bioRxiv - Biochemistry 2022Quote: ... The antibody-protein complexes were isolated by incubation with magnetic beads (Invitrogen Dynabeads protein A and protein G) for 6 hours at 4℃ ...
-
bioRxiv - Physiology 2023Quote: ... supernatant protein concentration was determined by BCA protein assay (Pierce BCA Protein Assay Kit, Cat#23225, Thermo Scientific). Twenty μg/lane was run (200V ...
-
bioRxiv - Molecular Biology 2024Quote: ... The supernatant containing nuclear proteins was measured for protein concentration using Pierce BCA Protein Assay Kit (Thermo Scientific) and stored at -80°C until use ...
-
bioRxiv - Bioengineering 2023Quote: ... Total protein concentration was determined using the Qubit™ Protein and Protein Broad Range (BR) Assay Kit (Invitrogen).
-
bioRxiv - Bioengineering 2023Quote: Protein yield: MSC-EV preparation total protein concentration was determined using the Micro BCA Protein Assay Kit (ThermoFisher) according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2024Quote: ... Protein concentration of each sample was determined using the BCA protein assay (Pierce™ BCA Protein Assay, Thermofisher). 40 micrograms of protein per lane were prepared in SDS sample buffer (50 mM Tris pH 6.8 ...
-
bioRxiv - Neuroscience 2024Quote: Protein concentration in the protein sample was determined by using a BCA protein assay kit (Pierce, Thermo Scientific). Samples in aliquots of 100 μL each were stored at -80°C.
-
bioRxiv - Cancer Biology 2021Quote: Proteins were quantified using a Pierce BCA Protein Assay Kit (ThermoFisher). Equivalent amounts of protein were resolved on precast polyacrylamide denaturing gels and were then transferred onto 0.2 μm PVDF membranes using either dry transfer with an iBLOT2 system (ThermoFisher ...
-
bioRxiv - Cancer Biology 2021Quote: ... Protein concentration was measured (Pierce BCA Protein Assay, Thermo Fisher Scientific) and a total of 100 µg of protein per sample was subjected for trypsin digestion ...