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Citations for Thermo Fisher :
1251 - 1300 of 10000+ citations for Probable U3 small nucleolar RNA associated protein 11 UTP11 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Protein targets were detected with primary antibodies against GAPDH (Invitrogen, #MA5-15738), Flag (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... the lysates were incubated with antibody-conjugated Protein G magnetic beads (Invitrogen) for 2.5 hours at 4°C ...
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bioRxiv - Immunology 2023Quote: ... were incubated with primary antibody pre-bound protein G agarose beads (Invitrogen) or with pre-blocked anti-FLAG M2 beads and rotated overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... tested antibodies were conjugated with AlexaFluor-647 using Protein Labelling Kit (Invitrogen) following manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... antibodies were first coupled with Protein G dynabeads (10003D, Thermo Fisher Scientific), and the sonicated chromatin was then incubated overnight at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... BB2 (inhouse purification) antibodies were crosslinked to Dynabeads protein G beads (Invitrogen) by incubating with 5 mM BS3 crosslinker (CovaChem ...
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bioRxiv - Microbiology 2021Quote: ... A mouse monoclonal anti-HSV 45-kDa protein (scaffolding protein) antibody (Serotec) at 1:2000 dilution and Alexa Fluor 568–goat anti-mouse IgG (Invitrogen) at 1:1000 dilution were used as primary and secondary antibodies ...
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bioRxiv - Plant Biology 2021Quote: ... The protein extracts were incubated overnight with polyclonal MdDREB2A antibody and then added protein A/G agarose beads (Thermo Fisher) to incubate at 4 °C for additional 4-5 hours ...
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bioRxiv - Microbiology 2020Quote: ... Proteins bound to GP96 were immunoprecipitated with an antibody against Grp94 (Rat, mAb, Enzo) and Dynabeads Protein G (Thermo Scientific), then examined using mass spectrometry analysis ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 viral nucleocapsid protein (NP) was detected using the anti-NP protein antibody (Cat. # PA5-81794, Thermo Fisher) diluted 1:10000 in 0.1% tween-20/1%BSA/PBS solution as a primary antibody ...
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bioRxiv - Genomics 2021Quote: ... The chromatin fragments bound to antibodies (after incubation overnight) were captured using 40 μl of protein-A or protein-G Dynabeads (Invitrogen; pre-washed with 0.1% BSA [Fraction V ...
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bioRxiv - Cell Biology 2022Quote: ... The antibodies and any bound proteins were purified by incubating the lysates with protein A/G magnetic beads (Thermo Scientific), washing the beads three times with lysis buffer and eluting bound proteins with 2% SDS in PBS.
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bioRxiv - Cell Biology 2022Quote: ... and interacting proteins were co-immunoprecipitated with 5 µg of anti-gag antibody per mg of total proteins using Dynabeads protein G (Invitrogen). Proteins were solubilized in Laemmli buffer and separated by SDS–PAGE ...
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bioRxiv - Cell Biology 2023Quote: ... Antibody–protein complexes were recovered during 2 h incubation at 4°C with protein A/G magnetic beads (Thermo Scientific), followed by duplicate washes of 15 min with low-salt wash buffer (0.1% SDS ...
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bioRxiv - Cancer Biology 2021Quote: ... three small cell culture dishes (2 mm grid, Nalge Nunc International, Rochester, USA) were filled with the cell preparation and placed in a large cell culture dish ...
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bioRxiv - Bioengineering 2021Quote: ... cells were partially dissociated to small aggregates with 1x TrypLE Express (Thermofisher 12605028) at 37 C followed by cell scraping ...
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bioRxiv - Cell Biology 2022Quote: ... small lung fragments of about 50 mg were homogenized with Trizol (ThermoFisher, 15596026). RNA extraction was performed as recommended by the manufacturer ...
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bioRxiv - Immunology 2020Quote: ... small Ubiquitin-Like Modifier (SUMO) protease (1 unit, 12588-018, Life Technologies, USA) was added to the reaction buffer (50 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2021Quote: ... hiPSCs were dissociated into small colonies using the gentle cell dissociation reagent (Gibco) and seeded on Geltrex matrix (Gibco ...
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bioRxiv - Genomics 2021Quote: ... Excised tissue was kept moist using a small volume of DPBS (Gibco #14287080). For large airways ...
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bioRxiv - Cancer Biology 2021Quote: ... tissues were sectioned in small pieces and placed in 0.25% trypsin solution (Invitrogen) in PBS and disaggregated by shaking during 90 min at 200 rpm and 37°C ...
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bioRxiv - Microbiology 2023Quote: Trigeminal ganglia were thawed and small sections homogenized and dissolved in TRIreagent (Ambion). DNA and RNA were extracted from the same sample according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... Each cortex was then cut into small pieces in Modified Eagle’s Medium (Thermofisher) supplemented with 10% FBS ...
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bioRxiv - Systems Biology 2024Quote: ... Small tissue biopsies or isolated cells were mixed with 250 µl PBS (Gibco) and 100 mg 0.1 mm silica beads (Biospec Products ...
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bioRxiv - Cell Biology 2024Quote: ... and a small amount of Fluoromount-G with DAPI (ThermoFisher 00-4959-52) was used to affix coverslips to the slides.
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bioRxiv - Cancer Biology 2022Quote: ... RNA pull-down assays were performed following the Pierce Magnetic RNA-Protein Pull-Down kit protocol (Pierce/Thermo Fisher Scientific, 20164). Biotinylated RNAs were pre-coupled to Nucleic Acid Compatible Streptavidin Magnetic Beads in RNA Capture Buffer (Pierce/Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: 200 μL of resuspended EV were used for RNA extraction using the total exosome RNA & Protein Isolation kit (Invitrogen, cat. # 4478545) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... 0.4pmol of biotinylated SINV RNA was incubated with normalized amounts of protein lysates and RNA binding buffer containing RNaseOUT (Thermo Fisher), heparin (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... The concentration of dsDNA in each miniprep was measured using the Qubit-4 fluorometer and the associated dsDNA high sensitivity assay kit (Invitrogen). Then ...
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bioRxiv - Microbiology 2021Quote: ... We measured the concentration of dsDNA in each miniprep using the Qubit-4 fluorometer and the associated dsDNA high sensitivity assay kit (Invitrogen). For each sample with a SpyCas9-expressing plasmid ...
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bioRxiv - Cancer Biology 2020Quote: mRNA associated with 1 to 3 ribosomes “Light polysomes” and mRNA associated with more than 3 ribosomes “Heavy polysomes” were extracted using TRIzol LS (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... PBMCs were reprogrammed into iPS cells using transient overexpression of pluripotency-associated factors (OCT4, KLF4, and SOX2) via non-integrating Sendai virus particles (Cytotune 2.0 kit, Life Technologies). At least five clones per TSC2+/+ and TSC2+/− donor were established into iPS cell lines ...
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bioRxiv - Cancer Biology 2024Quote: ... and MUG-Chor1 (CRL-3219) cell lines were purchased from ATCC and cultured in MesenPRO basal medium with an associated growth supplement (Invitrogen), GlutaMAX (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein concentration of the isolated exosomes was detected by using a total exosome RNA and Protein Isolation Kit (Thermo Fisher Scientific) and a BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: rox2RNA probes at 100 nM and hrp38 RNA probes at 50nM were incubated with MBP-tagged FL CLAMPWT protein or MBP-tagged FL CLAMPdelPrLD protein in REMSA binding buffer provided with the LightShift Chemiluminescent RNA EMSA kit (Thermo Scientific, USA) at room temperature for 30 min according to manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... The sections were rinsed in TBST and further incubated with the secondary antibody of goat anti-mouse FITC (1:500) or goat anti-rabbit Alexa Fluor-647(1:500) (Life Technologies, Thermo Scientific, USA) or goat anti-Guinea pig Alexa Fluor 555(1:500)for 2 h at room temperature in a humid chamber ...
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bioRxiv - Molecular Biology 2023Quote: ... was added to the cells and kept in 37°C humidified incubator for 1 h followed by incubation with fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse secondary antibody (Invitrogen, USA,1:500 dilution) and Alexa Fluor 594 conjugated goat anti-mouse (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissues were washed with TBST followed by incubation with the secondary antibody such as anti-mouse FITC-488 (1:1000) or goat anti-rabbit Alexa Fluor-647 (Life Technologies, Thermo Scientific, USA) at room temperature for 2 h ...
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bioRxiv - Cell Biology 2020Quote: ... Phosphopetides were desalted and then labelled with TMT 10/11-plex reagents (ThermoFisher) for subsequent quantitative proteomics ...
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bioRxiv - Genomics 2020Quote: ... we used 11 μL anti-rabbit or anti-mouse IgG Dynabeads (Life Technologies) and wash them with cold BSA/PBS (0.5 mg / mL bovine serum albumin in 1x phosphate buffered saline ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were analyzed by qPCR with TaqMan reagents (Applied Biosystems; Supplementary File 11), using 25 ng of cDNA per qPCR reaction ...
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bioRxiv - Microbiology 2020Quote: ... BF-2 and E-11 cells in 24 well cell culture plates (Gibco) and incubated at 25°C ...
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bioRxiv - Neuroscience 2021Quote: ... 11 μg pSPAX2 and 6.4 μg pVSVG using Lipofectamine 2000 (Thermo Fisher Scientific). The transfection media was replaced by plating media supplemented with 13 mg/mL bovine serum albumin (Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... we used 11 μL anti-rabbit or anti-mouse IgG Dynabeads (Life Technologies) and wash them with cold BSA/PBS (0.5 mg / mL bovine serum albumin in 1x phosphate buffered saline ...
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bioRxiv - Plant Biology 2021Quote: ... and digoxigenin 11-dUTP (35S) with a Nick Translation kit (Invitrogen - Oregon, USA).
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bioRxiv - Immunology 2022Quote: ... Bound phages were separated by streptavidin coated magnetic beads (Invitrogen, 11-205-D) and washed 10 times with 1 ml of PBS Ph 7.4 containing 0.1% Tween-20 (w/v) ...
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bioRxiv - Genomics 2022Quote: ... VCaP were cultured in DMEM F12: Ham 1:1 (Gibco; 11-320-033) containing 10% FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... were cultured and maintained as previously described63 in DMEM (Gibco 11-965-092) supplemented with 10% FBS (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... cells were cultured for an additional 11 d in neurobasal medium (Fisher Scientific) and 10 uM retinoic acid ...
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Hepatitis B Virus genomes associate with cellular sites of DNA damage by inducing replication stressbioRxiv - Microbiology 2024Quote: ... Oligos were pooled and labelled with 250 μM Amino-11-dUTP (Thermo Scientific) and TdT enzyme (Promega) ...