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Citations for Thermo Fisher :
1251 - 1300 of 10000+ citations for Phospholipase A and Acyltransferase 4 RARRES3 PLAAT4 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... RBD-Fc was biotinylated with EZ‐link sulfo‐NHS‐LC‐biotin (Thermo Fisher Scientific, Waltham, MA) (RBD-Fc-Bio) ...
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bioRxiv - Molecular Biology 2022Quote: ... Biotin signal on nylon membrane was detected using a streptavidin-based chemiluminescent method (Thermo Fisher Scientific).
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bioRxiv - Genomics 2022Quote: ... 100 μl of freshly prepared 25 mM EZ-Link™ Iodoacetyl-PEG2-Biotin (IPB, Thermo Scientific) was added to the solubilized cell particles to a final concentration of 5 mM and incubated at RT for 30 min in the darkness ...
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Dynamic chromatin organization and regulatory interactions in human endothelial cell differentiationbioRxiv - Developmental Biology 2022Quote: ... The pull-down of biotin adapter-containing DNA was performed using MyOne C1 Beads (ThermoFisher, 65001) for 30 minutes at room temperature with rotation ...
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bioRxiv - Immunology 2022Quote: ... Thioredoxin-fused peptides were chemically biotinylated with EZ-Link Sulfo-NHS-LC-biotin (Thermo Fisher Scientific).
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bioRxiv - Immunology 2022Quote: ... and purified anti-IFNγ and BsAb were biotinylated by EZ-Link Sulfo-NHS-SS-Biotin (ThermoFisher) and buffer exchanged by Zeba Spin Desalting Columns (7K MWCO ...
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bioRxiv - Cancer Biology 2022Quote: ... the αCD3 scFv monomer was labeled with EZ-Link Sulfo-NHS-LC-Biotin (Thermo Fisher, 21335) according to the manufacturer’s protocol and excess NHS-biotin was removed with 0.5 mL Zeba Spin Desalting Columns (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... Nascent proteins were labeled with biotin using the Click-it protein reaction buffer kit (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... surface receptors were labeled with Pierce™ Premium Grade Sulfo NHS-SS-Biotin (Thermofisher, Waltham, USA) and purified using Streptavidin High Performance Spintrap™ (Sigma-Aldrich ...
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bioRxiv - Biophysics 2021Quote: ... digoxigenin- and biotin-labeled sense and anti-sense primers (Integrated DNA Technologies, Coralville, IA, or Invitrogen, Life Technologies ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were dialyzed into bicarbonate buffer and biotinylated using EZ-LinkTM Sulfo-NHS-LC-Biotin (ThermoFisher) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... and Southern blots with the North2South Biotin Random Prime Labeling and Detection Kit (ThermoFisher cat. #17175) and the wild type hH2Az gene fragment used as a probe.
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bioRxiv - Developmental Biology 2020Quote: ... Synthesis of double-stranded cDNA and biotin-labelled cRNA was performed according to manufacturer’s instructions (Affymetrix). Fragmented cRNA preparations were hybridized to Drosophila genome oligonucleotide arrays (GeneChip Drosophila Genome 2.0 Array (3’ IVT Expression Affymetrix)] using an Affymetrix hybridization Oven 640 ...
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bioRxiv - Genomics 2021Quote: DNA ends were marked with biotin-14-dATP by adding 60μL of biotin fill-in master mix (1X NEB 3.1, 0.25mM dCTP, 0.25mM dGTP, 0.25mM dTTP, 0.25mM biotin-dATP (ThermoFisher#19524016), 50U Klenow polymerase Polymerase I (NEB M0210L)) ...
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bioRxiv - Genetics 2021Quote: ... Biotin-labelled probes were detected with Streptavidin-Alexa 660 (Thermo Fisher Scientific, Waltham, MA, United States); digoxigenin-labelled probes were detected with anti-digoxigenin-FITC (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... the slice storage solution was supplemented with 50 μM Sulfo-NHS EZ Link Biotin (Thermo Fisher) for 45 min before washing and further storage ...
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bioRxiv - Immunology 2021Quote: ... Purified ACE2 protein was biotinylated using the EZ-Link Sulfo-NHS-Biotin kit (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2020Quote: Old yeast cells were isolated by using EZ Link Sulfo-NHL-LC-LC-Biotin (Thermo Scientific) to label cell surface proteins (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... supernatant was discarded and fill-in mix was added (38 μM Biotin-14-dATP [Thermo Fisher Scientific] ...
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bioRxiv - Cell Biology 2022Quote: ... Sulfo-NHS-LC-LC-Biotin (Pierce) and Neutravidin agarose beads (Pierce) from Fisher Scientific (Waltham, MA). Culture media (Dulbecco’s Modified Eagle’s Media [DMEM] with 4.50 g/L glucose ...
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bioRxiv - Cancer Biology 2022Quote: ... Click-iT reaction cocktail with biotin-conjugated azide was prepared according to the manufacturer’s recommendations (Invitrogen), and cells were incubated for 80 min ...
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bioRxiv - Genetics 2022Quote: ... Amplification and biotin labeling of RNA was performed using GeneChip 3’ IVT express kit (Affymetrix, #901229). Affymetrix Drosophila Genome 2.0 array (# 900533 ...
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bioRxiv - Neuroscience 2023Quote: ... membranes were blocked 10% biotin-free fetal bovine serum (FBS, Fisher Scientific, Cat# 26-400-044) in TBST solution overnight at 4°C with mild agitation ...
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bioRxiv - Neuroscience 2022Quote: ... visualized by incubation with avidin-biotin-peroxidase complex (Immunopure ABC Peroxidase staining kit; Thermo Fisher Scientific), using the VectorSG Peroxidase Substrate Kit (Vector Laboratories ...
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bioRxiv - Molecular Biology 2024Quote: ... Reducing agents were then removed by filtering through Pierce dye and biotin removal column (Thermo Scientific). The resulting chains were then incubated overnight at room temperature with 10x molar excess of EZ-Link Maleimid-PEG2-Biotin (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... the purified antigens were then biotinylated with the EZ-Link Sulfo-NHS-Biotin (Thermo Fisher Scientific) using a 50:1 biotin to protein molar ratio for calculations.
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bioRxiv - Immunology 2024Quote: ... hDPP4-Fc and G2 were biotinylated using EZ-Link™ Sulfo-NHS-LC-Biotin (Thermo Scientific) and 80% maximum binding concentrations against S-2P were determined with biotinylated proteins (hDPP4-Fc-biot and G2-biot) ...
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bioRxiv - Immunology 2024Quote: ... after which free biotin was removed with 7K MWCO Zeba desalting spin columns (Thermo Fisher Scientific). Costar high binding 96-well assay plates (Corning ...
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bioRxiv - Biochemistry 2023Quote: ... Smurf1 HECT NT protein was biotinylated using the No-Weight NHS-PEO4-Biotin kit (Thermo Fisher) due to its compatibility with Super Streptavidin (SSA ...
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bioRxiv - Microbiology 2023Quote: ... The biotin-labeled probes were detected using a Chemiluminescent Nucleic Acid Detection Module (Thermo Scientific™) at RT ...
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bioRxiv - Biochemistry 2023Quote: ... and the biotin-labeled mRNA was detected by streptavidin Alexa Fluor™ 647 conjugate (Invitrogen™). To investigate the effect of IbpBEc on IbpAEc-mRNA interaction ...
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bioRxiv - Molecular Biology 2022Quote: ... EdU-PEG3-Biotin containing DNA molecules were affinity purified by Streptavidin MyOne C1 Magnetic Beads (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... 4sU-labeled RNA was then biotinylated with 1mg/ml EZ-link HPDP-Biotin (ThermoFisher, cat: 21341) for 90 min at room temperature as previously described34 ...
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bioRxiv - Molecular Biology 2023Quote: ... the overhangs in the restriction digested chromatin were filled in with biotin-14-dATP (ThermoFisher Scientific) using 30 U of DNA Polymerase I ...
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bioRxiv - Physiology 2023Quote: ... as previously done.26 AChR recycling was assessed by injecting α-bungarotoxin-Biotin (25 pmoles - Invitrogen) and saturating dose of streptavidin-Alexa647 (50 pmoles - Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... Biotinylation of the purified 3FTX was confirmed using the Pierce™ biotin quantitation kit (Thermo Fisher). Tetramers of streptavidin conjugated to biotinylated 3FTX were formed via 60 min incubation of 4:1 3FTX:streptavidin-APC (Thermo Fisher ...
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bioRxiv - Biochemistry 2023Quote: ... and the biotin-labeled mRNA was detected by streptavidin Alexa Fluor™ 647 conjugate (Invitrogen™).
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bioRxiv - Genetics 2023Quote: ... and treated with 0.3 mg/ml EZ-Link Sulfo-NHS Biotin (Thermo Fisher, Waltham, MA, USA) for 1 hr ...
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bioRxiv - Microbiology 2024Quote: ... heparin was biotinylated by mixing 2 mg heparin and 2 mg amine-PEG3-Biotin (Thermo Scientific), followed by the addition of 10 mg NaCNBH3 ...
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bioRxiv - Neuroscience 2024Quote: ... 0.1mM CaCl2 (pH 8.0)] were incubated with Sulfo-NHS-SS-biotin (1 mg/mL, Thermo Scientific) for 20 min at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: Biotin-labeled RNAs were synthesized using Scientific TranscriptAid T7 High Yield Transcription Kit (Thermo Fisher Scientific). Biotin-labeled RNA-pulldown assay was performed using Pierce™ Magnetic RNA-Protein Pull-Down Kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... Fresh medium containing the selected DSB agent and 50 μM D-Biotin (Thermo Fisher Scientific, B20656) were added and incubated for 1h ...
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bioRxiv - Genomics 2024Quote: ... Biotin enrichment was then performed by adding 20 μl of Dynabeads MyOne Streptavidin C1 (ThermoFisher 65001) and incubating at room temperature for 30 minutes with rotation ...
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bioRxiv - Microbiology 2024Quote: ... Purified ACE2 protein was biotinylated using the EZ-Link Sulfo-NHS-Biotin kit (Thermo Fisher Scientific) as reported previously 70.
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bioRxiv - Pathology 2024Quote: ... Biotin was detected using horseradish peroxidase (HRP)-conjugated streptavidin (Pierce, Life Technologies, Villebon-sur-Yvette, France) and the Supersignal West PicoChemiluminescent kit (Life Technologies) ...
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bioRxiv - Bioengineering 2024Quote: ... The biotinylation reagent (EZ-Link™ NHS-LC-LC-Biotin, Thermo Scientific 21343, in dimethyl sulfoxide) was then mixed in 12-fold excess molarity with the gelatin solution at room temperature for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... Surface proteins were prelabeled with 0.5 mg/ml membrane-impermeable Sulfo-NHS-SS-Biotin (Thermo Scientific) on ice for 15 min ...
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bioRxiv - Plant Biology 2022Quote: ... N-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino) phenyl) hexatrienyl) pyridinium dibromide (FM 4-64; 50 μM) (Invitrogen) or propidium iodide (PI ...
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bioRxiv - Biochemistry 2021Quote: ... Succinimidyl 6-(4, 4’-azipentanamido)hexanoate (LC-SDA; Thermo Scientific) crosslinking was performed by adding the reagent to final 1mM concentration ...
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bioRxiv - Neuroscience 2022Quote: ... A glass coverslip (4×4 mm, #1.5 thickness; Thermo Scientific) was positioned over two-thirds of the craniotomy ...