Labshake search
Citations for Thermo Fisher :
1251 - 1300 of 10000+ citations for 6 Methyl 2 3 4 9 tetrahydro 1H carbazol 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... 1-2 x 107 cells were incubated in 4 µM PBS-diluted CFSE (Invitrogen) for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... and 15 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Gibco, Gaithersburg, MD, USA). Cells were maintained at 37 °C in a humidified incubator with 5% CO2.
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bioRxiv - Biophysics 2024Quote: ... HEPES ((4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)) was obtained from Fisher Scientific (Pittsburg, PA). Peptides were reconstituted at 25 mg ml-1 in nuclease-free ultra pure water as per the manufacturer’s instructions and stored as aliquots at -20°.HP1α was reconstituted (0.5 mg ml-1 ...
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bioRxiv - Microbiology 2024Quote: ... for 1 h at 4°C.The DynaMag™-2 magnetic rack (Thermo Fisher Scientific) was used for flow-through removal and washing ...
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bioRxiv - Neuroscience 2024Quote: ... Peptides were loaded onto a C18 pre-column (3 μm, 75 mm i.d. × 2 cm, 100 Å, Thermo Fisher Scientific Cat# 16-494-6) then separated on a reverse-phase nano-spray column (2 μm ...
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bioRxiv - Genomics 2024Quote: ... HAECs at low passage (passage 3-6) were treated prior to harvest every 2 days for 7 days with either 10 ng/mL TGFB2 (ThermoFisher Scientific, cat. no. 302B2002CF), IL1B (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... The lungs were inflated with 1-3 ml 2% UltraPure Low Melting Point Agarose (Invitrogen). Lungs and livers were fixed overnight at 4°C on a shaker ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... #2: 5’-gatcccGATTACTCGGCCATGATCAAAgcttcctgtcacTTTGATCATGGCCGAGTAATCttt ttta-3’ and 5’-agcttaaaaaaGATTACTCGGCCATGATCAAAgtgacaggaagcTTTGATCATGGCCGAGTAATgg-3’ were purchased from Invitrogen. The DNA oligo pairs were annealed and inserted into pEntryCla12-chickU6 shuttle vector using BamHI/HindIII site.
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed 2-3 times (200 rpm, 5 min at 4°C) in eBioscience™ Permeabilization buffer (250 µl/well) (Invitrogen) and resuspended in eBioscience™ Fixation/Permeabilization solution (Invitrogen ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: HeLa cell pellets (∼2×107) or MCF7 cell pellets (∼4×107) were lysed with 3 mL Mammalian Protein Extraction Reagent (Thermo Scientific), supplemented with 2X HALT protease inhibitor (Thermo Scientific) ...
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bioRxiv - Genomics 2019Quote: ... 2 and 4 h post dosing on day 3 and collected on ice in microcentrifuge tubes containing K2-EDTA (Fisher Scientific). Within 30 min after collection ...
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bioRxiv - Molecular Biology 2022Quote: ... 3-4 mg total protein and 2 ug of KIF1C antibody or 10 ug mCherry antibody (Invitrogen mCherry Monoclonal Antibody (16D7)) were used ...
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The effects of caloric restriction on adipose tissue and metabolic health are sex- and age-dependentbioRxiv - Physiology 2023Quote: ... Tissues in formalin were fixed at 4°C for 2-3 days before being washed and stored in DPBS (Life Technologies). Tissues on dry ice were stored at −80°C prior to downstream analysis.
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bioRxiv - Neuroscience 2024Quote: ... diluted in TBST at RT for 1h and then incubated for 1h at RT with appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies (Invitrogen). The visualization of the signal was conducted using Immobilon Forte Western HRP substrate (Millipore ...
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bioRxiv - Developmental Biology 2021Quote: ... methyl ester (TMRM) (100nM, Thermo fisher Scientific) for 30 mins at room temperature ...
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bioRxiv - Physiology 2021Quote: ... 0.1% (w/v) methyl violet (Fisher Scientific), diluted in H2O) ...
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bioRxiv - Cell Biology 2022Quote: ... containing 100nM tetramethylrhodamine methyl ester (TMRM) (Invitrogen), 10ug/mL Hoescht (Thermo Scientific) ...
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bioRxiv - Cell Biology 2023Quote: Tetramethyl rhodamine methyl ester (TMRM) (ThermoFisher, #T668) is a cell-permanent dye that accumulates in active mitochondria with intact membrane potentials ...
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bioRxiv - Neuroscience 2023Quote: ... Bodipy TR methyl ester (Thermo Fisher Scientific) diluted 1:300 in PDT (0.1% Triton-X100 and 1% DMSO in PBS ...
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bioRxiv - Physiology 2023Quote: ... methyl ester (TMRM) (Thermo Fisher Scientific #T668) at 37°C for 15min ...
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bioRxiv - Bioengineering 2024Quote: ... specifically with TMRM (Tetramethylrhodamine, Methyl Ester, ThermoFisher) at a final concentration of 100 nM and Lysotracker Deep Red (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... methyl ester (TMRM) dye (T668, Invitrogen, Belgium). The following viability assay kits were used ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.5 mg/ml hydrocortisone (Cat#1H-0888, Invitrogen), 100 ng/ml cholera toxin (Cat#101B ...
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bioRxiv - Biochemistry 2021Quote: ... followed by 6 column volumes of elution buffer (2× GIBCO 14200-075 PBS ...
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DNA uptake by cell wall-deficient bacteria reveals a putative ancient macromolecule uptake mechanismbioRxiv - Microbiology 2022Quote: ... 10 mM Laurdan (6-Dodecanoyl-2-Dimethylaminonapthalene) stock solution (Invitrogen) was prepared in 100% dimethylformamide (DMF ...
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bioRxiv - Molecular Biology 2020Quote: ... pH 6 using a 2 kDa MWCO dialysis unit (ThermoFisher). Heats of binding were measured using a MicroCal iTC200 calorimeter (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... 6 - diamidino-2-phenylindole (DAPI, 5 µg/ml, Life Technologies).
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bioRxiv - Biophysics 2022Quote: ... and 6-Dodecanoyl-2-Dimethylaminonaphthalene (Laurdan) were purchased from ThermoFisher Scientific Ltd ...
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bioRxiv - Cancer Biology 2022Quote: ... 6-diamidino-2-phenylindole (DAPI) for nuclear detection (ThermoFisher MA). For live-cell imaging ...
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bioRxiv - Microbiology 2024Quote: ... 6’-diamidino-2 phenylindole (DAPI; Cat. P36941, Invitrogen, Carlsbad, CA) and visualized on a Leica Stellaris confocal microscope using a 63x oil immersion objective ...
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bioRxiv - Neuroscience 2024Quote: ... incubated with DAPI (40, 6-diamidino-2-phenylindole, Invitrogen, D1306) (0.5mg/ml in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... + 2% heat inactivated fetal bovine serum Certified One Shot (Gibco A38400-01) + 1× N-2 supplement ...
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bioRxiv - Genomics 2021Quote: ... on the Ion One Touch™ 2 System (Thermo Fisher Scientific, USA). Template-positive ISPs were enriched on Ion One Touch™ ES system (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... coli One Shot ccdB Survival 2 TIR competent cells (Invitrogen, ThermoFisher Scientific) and selected on LB agar supplemented with 50 µg/mL kanamycin ...
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bioRxiv - Biochemistry 2022Quote: ... coli One Shot ccdB Survival 2 TIR competent cells (Invitrogen, ThermoFisher Scientific) and selected on LB agar supplemented with 50 µg/mL kanamycin ...
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bioRxiv - Biochemistry 2024Quote: ... coli One Shot™ ccdB Survival™ 2 T1R Competent Cells (ThermoFisher). Single colonies were cultured for plasmid isolation and the cloned sequence was confirmed by DNA sequencing.
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bioRxiv - Microbiology 2022Quote: ... coli One Shot® ccdB SurvivalTM 2 T1R competent cells (Life Technologies), which was grown in LB agar plates (10 g l-1 NaCl ...
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bioRxiv - Biophysics 2020Quote: All the in vitro transcription experiments were performed in NEB’s transcription buffer (40 mM Tris-HCl, 6 mM MgCl2, 1 mM DTT, 2 mM spermidine) with 1 mM NTPs (R1481, Thermo Scientific), 22 wt% glycerol ...
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bioRxiv - Microbiology 2020Quote: The lipophilic fluorescent membrane probe 1-(4-Trimethylammoniumphenyl)-6-Phenyl-1,3,5-Hexatriene p-Toluenesulfonate (TMA-DPH) (Themo Fisher Scientific) was used to assess EV associated lipids ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were blocked in TBS-T/5% BSA for one hour at RT and incubated overnight at 4 °C with anti-CD63 (TS63) (1:500, #10628D, Life Technologies), anti-CD171 (L1CAM ...
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bioRxiv - Developmental Biology 2022Quote: ... and incubated overnight at 4°C with 1 mg/ml BSA and 4% sheep serum in PBST and one or more of the following: Alexa-fluor 488 Phalloidin 1:300 (Molecular Probes), anti-beta tubulin antibody 1:100 (E7 ...
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bioRxiv - Molecular Biology 2020Quote: ... for the first 24 hours and for further 4 to 6 days in Essential 6 media (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2019Quote: ... HEK293t were replated every 3 days at a split ratio of 1 in 6 following dissociation with Trypsin 0.05% EDTA (Gibco, Life technologies).
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bioRxiv - Synthetic Biology 2021Quote: ... 200 ng of nLYN-mCherry and 500 ng of iLID plasmid were mixed with 2.1 ul (1 to 3 ratio) of Fugene 6 in 125 ul of Opti-MEM medium (Thermofisher Scientific). After 10 minutes incubation at room temperature ...
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bioRxiv - Genetics 2023Quote: 3 independent total RNA extractions from 30 ovaries from 3-6-day-old RevI-H2i2 flies using Trizol (Invitrogen) were performed ...
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bioRxiv - Immunology 2022Quote: Total RNA was prepared from one lung lobe collected at 3 dpi using lysing matrix D (MP Biomedical) containing 1 ml of TRIzol reagent (Ambion, Life technologies) and homogenization at 20 s at 4.0 m/s twice using MP Biomedical Fastprep 24 Tissue Homogenizer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The transfection reagent was mixed with the pOG44 Flp-Recombinase expression vector and the pCDNA5/TO/FRT-3xMyc-eGFP-CLIP-170 WT or one of the mutant vectors with a 3:1 ratio in Opti-MEMTM I medium (Gibco, ThermoFisher Scientific). Selection started 48h after the transfection in a Dulbecco’s modified Eagle medium (DMEM ...
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bioRxiv - Neuroscience 2020Quote: ... and miR-9/9*-124 + TuD-miR-124 using TRIzol Reagent (Invitrogen, 15596026) in combination with RNeasy micro kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... and the bacterial pellets resuspended in PBS and supplemented with a 1:1 ratio of 2X LIVE/DEAD solution (6 μM SYTO-9 stain and 30 μM propidium iodide) from LIVE/DEAD® BacLight kit (Invitrogen). After incubation in the dark for 15 min ...
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bioRxiv - Cell Biology 2019Quote: ... RNA was isolated from whole eyes of 6-month old cep290 mutant and sibling control animals (n = 9 per group) with Trizol (ThermoFisher 15596026). Reverse transcription using 1 μg RNA was performed with the iScript cDNA Synthesis kit (Bio-Rad 1708891) ...