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Citations for Thermo Fisher :
1251 - 1300 of 10000+ citations for 6 FLUOROCHROMANO 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... employing a Step One plus thermocycler (Applied Biosystems). The results were normalized and expressed as fold changes in gene expression levels relative to control cDNA (2-ΔΔCT method) ...
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bioRxiv - Cancer Biology 2023Quote: ... one drop of Hoechst 33342 Ready Flow (Invitrogen) was added and cells were returned to the incubator for 1h at 37 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... with 10% FBS (Gibco One-Shot FBS, UK) (charcoal stripped to deplete hormones) ...
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Treg cells drive MYCN-mediated immunosuppression and tumor aggressiveness in high-risk neuroblastomabioRxiv - Cancer Biology 2023Quote: ... and a Step-One PCR instrument (Applied Biosystems) were utilized for the qRT-PCR reaction ...
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bioRxiv - Cancer Biology 2023Quote: ... Step-one Real-time PCR system (Life Technologies) or Bio-Rad C1000 Thermal Cycler (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: ... and one-part DMEM/F12 (Fisher Scientific; 11320033) supplemented with 5 μg/ml insulin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... coli One Shot BL21 Star (DE3) cells (Invitrogen). The unlabeled expression was carried out in the Terrific Broth medium (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... 5-(and-6)-carboxyfluorescein (FAM) and 5-(and-6)-carboxytetramethylrhodamine (TAMRA) were obtained from Invitrogen™ ...
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bioRxiv - Plant Biology 2023Quote: ... 6 uL of 0.5 M EGTA (bioWORLD) and 6 uL of 0.5 M EDTA (Invitrogen) were added to quench the reaction at 65 °C for 10min ...
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bioRxiv - Cancer Biology 2022Quote: ... Five million C4-2 or two million SK-OV-3 cells in serum free media were mixed one to one with growth factor reduced Matrigel® (Invitrogen) in a total volume of 200 μl and injected in the hind flank using a 23- or 27-gauge needle ...
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bioRxiv - Microbiology 2020Quote: ... and 6 μl of the 2X Luna Universal One-Step Reaction Mix was subjected to one-step RT-qPCR using Applied Biosystems QuantStudio 3 (ThermoFisher Scientific), with the following cycling conditions ...
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bioRxiv - Microbiology 2021Quote: ... were performed with the Express one step RT-qPCR Universal kit (EXPRESS One-Step Superscript™ qRT-PCR Kit, universal Invitrogen) using 3.5µL of RNA and 6.5µL of RT qPCR mix and standard fast cycling parameters ...
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bioRxiv - Microbiology 2019Quote: ... The extracted RNA was reversely transcribed and amplified by a one-step RT-qPCR using a 48-well plate real-time PCR system Step-One (Applied Biosystems). The sets of primers and probes used for the VP1 and for the Elongation Factor −1 alpha (ELF1α ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... We did One-step real-time RT-PCR (Step-One Plus real-time PCR thermocycler, Applied Biosystems, Foster City, CA, USA), using total nucleic acids extracted by Trizol® (Invitrogen ...
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bioRxiv - Physiology 2022Quote: ... and the concentration was measured using a NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer (Thermo Fisher Scientific ND-ONE-W). Amplification of 300 ng of DNA was performed using KOD Hot Start Master Mix (Sigma-Aldrich 71842) ...
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bioRxiv - Pathology 2024Quote: ... After one last freeze-thaw cycle the total protein content was measured on the NanoDrop One Microvolume UV-Vis Spectrophotometer (ThermoFisher Scientific).
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bioRxiv - Pathology 2024Quote: ... Kidneys were divided longitudinally with one half stored in formalin for histological evaluation and one half placed in RNALater (Thermofisher Scientific). Sham animals underwent a midline laparotomy alone with no renal pedicle clamping ...
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bioRxiv - Genetics 2023Quote: ... according to the manufacturer’s protocol and normalized to the correct concentration using the NanoDrop One Spectrophotometer (Thermo Scientific, ND-ONE-W). Samples were then submitted to the Center for Genome Sciences and Systems Biology (Washington University in St ...
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bioRxiv - Microbiology 2024Quote: ... extracted RNA was used as a template in a cycle-limited one-step RT-PCR reaction (SuperScript IV One-Step, Thermo Fisher) using custom primers (Table 2 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells nuclei were counter-stained using 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI) mounting medium (ProLong™ Diamond Antifade Mountant with DAPI, Thermo Fisher Scientific, Warsaw, Poland). Preparations were observed using the confocal microscope under magnification 630× and processed with Fiji software (ImageJ 1.52n ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4’,6-diamidino-2-phenylindole (DAPI) (00-4959-52, Invitrogen, Thermo Fisher Scientific, MA, USA) as counterstaining.
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bioRxiv - Molecular Biology 2022Quote: A concentration of 6 × 103 cells was loaded with 5 mM 4-amino-5-methylamino-2’,7’- difluorofluorescein diacetate (DAF-FM, Molecular Probes, Thermo Fisher, Sao Paulo, Brazil) after 72 h of treatment ...
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bioRxiv - Cancer Biology 2019Quote: ... and 5-6 pieces per well were cultured in a 6-well culture plate (Fisher Scientific). For up to two weeks tissue pieces were cultured in Advanced Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Microbiology 2021Quote: ... using 6 mL of cell suspension and 6 mL of Prolong Gold Antifade mountant (ThermoFisher Scientific) that were mixed on an object glass and then covered with a special STED cover glass ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Plasma IL-6 levels were measured by mouse IL-6 ELISA kit (ThermoFisher Scientific, Waltham, MA).
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bioRxiv - Immunology 2022Quote: ... IL-6 measurement was performed using IL-6 Mouse Uncoated ELISA Kit (88-7064-22; Invitrogen).
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bioRxiv - Neuroscience 2023Quote: ... Serum was run through an interleukin-6 (IL-6) enzyme-linked immunoabsorbent assay (ELISA) kit (Invitrogen). Successful maternal immune activation in offspring of Poly(I:C ...
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bioRxiv - Developmental Biology 2022Quote: ... bTSCs were passaged every 6 days at a 1:6 split ratio using Accutase (Gibco, A1110501). Each well of bTSCs was dissociated by 1 mL Accutase for 5 mins at 38.5 °C ...
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bioRxiv - Microbiology 2021Quote: One-step RT-PCR was performed on 2ul total RNA using the SuperScript IV One-Step PCR kit (ThermoFisher, Catalog No. 12594025) either to amplify nucleotides 1232-1583 of the PPRV N gene using published primers NP3 and NP4 (Couacy-Hymann et al 2002 ...
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bioRxiv - Microbiology 2021Quote: ... Amplification of RNA samples was performed with one-step TaqMan RT-qPCR using the RNA UltraSense One-Step quantitative system (Thermo Fisher Scientific). The standard curve was generated by serial end-point dilution ...
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bioRxiv - Systems Biology 2020Quote: ... The samples corresponding to either one TMT11plex or one TMTPro set were then mixed in a single glass HPLC insert (ThermoFisher C4010-630) and dried down to dryness in a speed-vacuum (Eppendorf ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR were performed with SuperScript III Platinum One-Step qRT-PCR kit (SuperScript™ III Platinum™ One-Step qRT-PCR kit, universal Invitrogen) using 5μL of nucleic acid extract and 20μL of RT-qPCR reagent mix using cycling parameters ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR were performed with SuperScript III Platinum One-Step qRT-PCR kit (SuperScript™ III Platinum™ One-Step qRT-PCR kit, universal Invitrogen) using 2.5μL of nucleic acid extract and 7.5μL of RT-qPCR reagent mix ...
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bioRxiv - Genomics 2022Quote: ... Plasma protein absorbance was measured at 280 nm using NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer (Thermo Scientific, ND-ONE-W).
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bioRxiv - Developmental Biology 2024Quote: ... Triplicate reactions for each sample were prepared and the samples were run in a Step One Real-Time PCR Step One Real-Time PCR System Thermal Cycling Block (Applied Biosystems #4376357). The analysis of real-time data was performed using the included StepOne Software version 2.0 and graphs were generated using GraphPad Prism (GraphPad Software) ...
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bioRxiv - Microbiology 2023Quote: ... An 800 bp fragment covering the ORF10 region was amplified by one-step RT-PCR using the SuperScript™ IV One-Step RT-PCR System (Invitrogen™, ThermoFisher) and the following primers (OL11F ...
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bioRxiv - Cell Biology 2023Quote: ... 5 μl of extracted RNA samples were determined in one-step RT-qPCR using AgPath-ID one-step RT-PCR (Applied Biosystems, UK) with the following cycle conditions ...
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bioRxiv - Microbiology 2024Quote: ... the extracted vRNA was converted to double-stranded cDNA and amplified using a one-step RT-PCR using SuperScript III One-Step RT-PCR kit (Thermo Fisher Scientific). The primers used were as follows ...
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bioRxiv - Genomics 2024Quote: ... Emulsion PCR for sequence template synthesis was performed with the Ion PITM Hi-QTM OT2 200 Kit v2 using the Ion One TouchTM II System and the Ion One TouchTM II ES (Thermo Fisher Scientific). Sequencing was performed using the Ion PITM Chip kit v3 and the Ion ProtonTM System (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2024Quote: ... Emulsion PCR for sequence template synthesis was performed using the Ion PITM Hi-QTM OT2 200 Kit v2 with the Ion One TouchTM II System and the Ion One TouchTM II ES (Thermo Fisher Scientific). Sequencing was performed using the Ion ProtonTM System with Ion PITM Chip kit v3 (Thermo Fisher Scientific) ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... with 4% sucrose/4% formaldehyde (ThermoFisher Scientific) in 1X PBS ...
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bioRxiv - Cell Biology 2020Quote: ... 6 ml basic F12 media (GIBCO) was added and 500 μl of FBS (Hyclone ...
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bioRxiv - Developmental Biology 2019Quote: ... and dispase (6 mg/mL, Gibco) for 1 h at 4 °C ...
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bioRxiv - Neuroscience 2019Quote: ... IL-6 (20 ng/mL, ThermoFisher). To generate human induced pluripotent stem cells (iPSCs) ...
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bioRxiv - Cancer Biology 2019Quote: ... Then 6 μl Lipofectamine RNAiMAX (Invitrogen) was added and supplemented to a total volume of 250 μl with optiMEM ...
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bioRxiv - Immunology 2021Quote: ... using QuantStudio 6 platform (ThermoFisher Scientific). Ct values were normalized to the Ct values for Ecad (Btln1,4,6,Il15 ...
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bioRxiv - Genomics 2021Quote: ... Empty 6% TBE gel (EC62655BOX, Invitrogen) was run at 100V in 0.5x UltraPure TBE Buffer (15581-044 ...
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bioRxiv - Immunology 2022Quote: ... 2DG (Thermo Fisher, 6 g/L) was dissolved in drinking water and was provided to mice ad libitum ...
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bioRxiv - Immunology 2020Quote: ... and 6 μg/ml CpG (Invitrogen) in complete RPMI in an incubator at 37°C/5% CO2 for 5 days ...
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bioRxiv - Microbiology 2021Quote: ... 6 µl dNTPs 2.5 mM (Invitrogen), and 2 U of AMV RT (Life Sciences ...