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Citations for Thermo Fisher :
1251 - 1300 of 10000+ citations for 6 Chloro 2 3 diphenylimidazo 1 2 b pyridazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... D13+14 PT-enhanced organoids (triplicate wells per condition) were incubated in 4’,6-diamindino-2-phenylindole substrate (DAPI; 1:1000 [Thermo Fisher Scientific]) with 1:500 DRAQ7 dead cell label (Thermo Fisher Scientific] ...
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bioRxiv - Genetics 2022Quote: ... These pieces were transferred to the lower surface of a 25 cm2 culture flask (6-8 pieces per flask) which had been pre-moistened with 1-2 mL of AmnioMAX Complete Medium (Thermo Scientific, #11269016) supplemented with 1% penicillin/streptomycin (GenDepot ...
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bioRxiv - Cell Biology 2022Quote: ... cells were labeled with DAPI together with the secondary antibody (4’,6-Diamidino-2-Phenylindole, Dihydrochloride, 1:10.000, Thermo Fisher Scientific, #D1306). The following secondary antibodies were used ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were transfected for 6 or 24 hours with Imp1-targeting siRNAs (siRNA #1: s155193, or siRNA #2: s155193. Both from Ambion, Thermo Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: ... The spike of the SARS-CoV-2.SctΔ19 B.1.617.2 (delta) virus was generated (GeneArt Gene Synthesis, ThermoFisher Scientific) from the full protein sequence of the original SARS-CoV-2 isolate Wuhan-Hu-1 (WH1 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were selected for 2 weeks in DMEM supplemented with hygromycin B (250 μg/ml; Thermo Fisher Scientific) and blasticidin (4 μg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... cultured with neuronal maturation medium (BrainPhys Basal Medium [Stemcell Technologies], B-27 and N-2 supplements [Thermo Fisher Scientific] ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Slices were finally incubated for 3-6 minutes with DAPI (1:10 000, Invitrogen) diluted in PBS and mounted on Polysine® Slides stored at 4°C until imaging.
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bioRxiv - Neuroscience 2022Quote: ... Hippocampal neurons 13-15 days-in-vitro (DIV) were transfected with plasmid (2 h, 2-3 µg per dish) with Lipofectamine 2000 (Invitrogen, #52887, minimum 24 h). For pH imaging experiments ...
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bioRxiv - Genomics 2019Quote: ... Round 2 and 3 PCRs were followed in real time using SYBR Green (Invitrogen) and stopped prior to plateauing ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were passaged every 2-3 days by washing with HBSS (Gibco, cat#14170), trypsinizing using 0.25% Trypsin-EDTA (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MMT) was obtained from Thermo Fisher. Crystal violet was purchased from VWR ...
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bioRxiv - Bioengineering 2020Quote: Nbs were diluted in a 2- or 3-fold series in Opti-MEM (Thermofisher). Each Nb dilution (110 μl ...
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bioRxiv - Physiology 2021Quote: ... cells were fed every 2-3 days with DMEM + 10% FBS (Thermo fisher Scientific) until differentiation was complete at day 10.
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bioRxiv - Immunology 2022Quote: ... The band are visualized with gel electrophoresis using 2-3% agarose (#16500500, Thermo Fisher) gel with SYBR Safe dye (#S33102 ...
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bioRxiv - Neuroscience 2021Quote: ... and 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium Chloride (INT, #I00671G, Fisher Scientific).
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bioRxiv - Genomics 2019Quote: ... Round 2 and 3 PCRs were followed in real time using SYBR Green (Invitrogen) and stopped prior to plateauing ...
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bioRxiv - Neuroscience 2022Quote: ... Approximately 2-3 sections were mounted on each Superfrost Plus slide (Thermo Fisher Scientific). All sections were air-dried at −20 ° C for 1 hour and afterwards stored at −80 ° C.
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bioRxiv - Genomics 2023Quote: ... use 10 μL for 2-3 ml of blood) or EDTA (0.5M EDTA, Gibco, cat ...
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bioRxiv - Biophysics 2023Quote: ... 2’(3’)-O-(2,4,6-trinitrophenyl)-adenosine 5’-triphosphate (TNP-ATP) was purchased from Invitrogen as a trisodium salt and stored in the dark at -20 °C at 10 mg/ml_ ...
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bioRxiv - Systems Biology 2024Quote: ... Cells were passaged every 2-3 days using 0.05% Trypsin/EDTA (Gibco™, 25300062). Cell density was 5x 104 per well for the experiments in the μ-Slide (ibidi ...
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bioRxiv - Cell Biology 2023Quote: ... MCEC were split every 2-3 days using TrypLE express enzyme (GibcoTM, Thermo Scientific) for 5min at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT, Thermo Scientific) assay was performed on cells seeded and treated in 96 plates for 72 hours with the free doxorubicin or doxo-EVs ...
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bioRxiv - Neuroscience 2023Quote: FOs from 2 or 3 independent differentiations were fixed using 4% paraformaldehyde (Thermo Scientific) in PBS (Gibco ...
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bioRxiv - Genetics 2024Quote: ... and 2 µl of DNA using QuantStudioTM 3 real-time PCR system (ThermoFisher Scientific). For the amplification of GLS promoter region upstream of the repeat ...
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bioRxiv - Immunology 2021Quote: ... Glucose uptake was measured by the uptake of glucose analogue 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG, Life Technologies). Cell surface and intracellular cytokine stainings of splenocytes and blood lymphocytes were performed as described (42) ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated in glucose-free media containing 5 μg/ml 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher) and 2.5% FBS at 37 °C ...
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bioRxiv - Physiology 2020Quote: ... 1 mM fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Life Technologies) was diluted in Live Cell Imaging Solution (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: Cellular glucose uptake was measured by culturing cells in glucose free DMEM for 2 hours before adding 100µM of the fluorescent glucose analogue 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl) Amino)-2-Deoxyglucose (2-NBDG; ThermoFisher, N13195). The analogue was incubated with MCF7s for 30 minutes and MSCs for 60 minutes at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM Tris(2-carboxyethyl)phosphine (TCEP, ThermoFisher)) supplemented with a protease inhibitor tablet (Roche ...
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bioRxiv - Genetics 2019Quote: ... 2 μl of probe (2 μM; Applied Biosystems) and 2 μl of template DNA (where extracts were normalised to 34 ng/μl) ...
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bioRxiv - Cell Biology 2020Quote: ... 2 X 105 PC3 or MCF7 cells were plated in either 6 well culture dishes (Nunc™ ...
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bioRxiv - Immunology 2019Quote: ... Tissue was washed for 24h before nuclear staining with 4′,6-diamidino-2-phenylindole (DAPI, ThermoFisher). Images were acquired using a Zeiss LSM 710 confocal (Carl Zeiss) ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in PBS with 30nM of 4’,6-diamidino-2-phenylindole (DAPI, Life Technologies). In each tube ...
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bioRxiv - Molecular Biology 2020Quote: ... 6’-diamidino-2-phenylindole (DAPI) for nuclear staining (Fluoromount-G™ Mounting Medium, with DAPI, Invitrogen), and incubated overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... 50 μL of 2-6 μg/mL S was plated onto 384-well Nunc Maxisorp (ThermoFisher) plates in PBS and sealed overnight at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... slices were stained by 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI: Thermo Fisher Scientific, Yokohama, Japan) across several experiments ...
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bioRxiv - Molecular Biology 2022Quote: ... and then the nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific) for 5 min ...
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Single-cell protein analysis reveals metastable states during the transition to a sensory organ fatebioRxiv - Developmental Biology 2021Quote: ... Then they were stained with 0.5 μg/ml 4’,6-diamidino-2-phenylindole (DAPI, Thermo Scientific), and mounted in Vectashield (Vector Labs) ...
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bioRxiv - Microbiology 2021Quote: ... and stained with 200 μl of 1.0 μg/ml 4’,6-diamidino-2-phenylindole (DAPI; Invitrogen) diluted in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Nuclei were counterstained using 5μg/ml of DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride; D1306, Invitrogen) added during secondary antibody incubation.
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bioRxiv - Cancer Biology 2021Quote: ... : DAPI Buffer (106mM MgCl2, 50 µg/mL 4’, 6-diamidino-2-phenylindole (DAPI, Invitrogen, Cat# D1306), 5mM Ethylenediaminetetraacetic acid (EDTA ...
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bioRxiv - Physiology 2022Quote: ... CellTracker Green CMFDA Dye (C2925) and DAPI (4’,6-diamidino-2-phenylindole) (D1306) were from Invitrogen. EZ-Link Sulfo-NHS-LC-LC-Biotin (#21135 ...
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bioRxiv - Cancer Biology 2020Quote: ... and Type 6 primary probes targeting MYCN were designed and synthesized by Affymetrix (Supplementary Table 2). Hybridization was performed according to the manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Nuclei were then stained with 0.01% w/v 4’,6-diamidino-2-phenylindole dilactate (DAPI, Invitrogen) for 5 min and rinsed with ddH2O ...
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bioRxiv - Neuroscience 2022Quote: ... Non-viable cells were excluded by staining with 4’,6-diamidino-2-phenylindole (Thermo Fisher, D1306). Flow-cytometric data were analyzed using FlowJo software (BD Biosciences).
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bioRxiv - Cell Biology 2020Quote: ... Nuclei were counterstained with 300nM 4’,6-diamidino-2-phenylindole dilactate (DAPI) (D1306, ThermoFisher Scientific, UK) for imaging assessment (Zeiss Axio observer Z1 microscope) ...
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bioRxiv - Microbiology 2019Quote: ... Coverslips were stained with 300 nM 4’,6-diamidino-2-phenylindole nucleic acid stain (DAPI; Invitrogen) in PBS for 5 min followed by washing three times in PBS at room temperature ...
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bioRxiv - Immunology 2021Quote: ... however Live/Dead stain was not performed and instead 4’-6’-diamidino-2-phenylindole (DAPI, ThermoFisher) was added immediately prior to sorting for live/dead detection ...
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bioRxiv - Neuroscience 2020Quote: ... 4’,6-diamidino-2-phenylindole (DAPI) and Leica standard immersion oil were obtained from Thermo Fisher Scientific ...