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Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for 6 Chloro 2 3 diphenylimidazo 1 2 b pyridazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Grids were blotted for 2 - 6 s in a Vitrobot (Mark IV, Thermo Fisher Scientific) at 4 °C and 100% humidity ...
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bioRxiv - Neuroscience 2022Quote: ... A nuclear counterstaining was performed using 4ʹ,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific) in TBS for 15 min ...
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bioRxiv - Cancer Biology 2022Quote: ... and mounted with Prolong Gold Antifade with 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen, P36935) as a counterstain for nuclei ...
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bioRxiv - Biochemistry 2023Quote: ... Nuclear counterstain was performed with 4’,6-diamidino-2-phenylindole (DAPI) fluorescent stain (Invitrogen, P36935). Cells were analyzed by confocal microscopy (LSM700 laser scanning microscope ...
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bioRxiv - Microbiology 2023Quote: ... The cell nuclei were stained with DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride) (Life Technologies) at a dilution of 1:5000 in 1X PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were further incubated with DAPI (4’,6-diamidino-2-phenylindole, 5µg/ml, Invitrogen D1306) to stain nuclei prior to imaging ...
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bioRxiv - Bioengineering 2023Quote: ... 50 mg Sulfo-SANPAH (sulfosuccinimidyl 6-(4’-azido-2’- nitrophenylamino)hexanoate) (cat. 22589, ThermoFisher Scientific) was dissolved in 1 ml DMSO and diluted 25-times in Milli-Q water ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were stained with 4’,6-Diamidino-2-Phenylindole (DAPI, 5µg/ml, DAPI, ThermoFisher D1306) in PBS for 5 min at RT ...
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bioRxiv - Microbiology 2023Quote: ... Parasites were mounted with ProLong diamond with 4’,6’-diamidino-2-phenylindole (DAPI) (P36931; Invitrogen) and imaged on an Axio Observer 7 equipped with an Axiocam 702 mono camera and Zen 2.6 Pro software (Zeiss ...
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bioRxiv - Neuroscience 2024Quote: ... samples were also counterstained with DAPI (4′,6-diamidino-2-phenylindole; ThermoFisher Scientific, Waltham, MA) to visualize nuclei and/or CellMask (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... MOI coverslips were incubated with 300 nM 4’ 6-diamidino-2-phenylindole (DAPI; [D1306] Invitrogen) in PBS for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... Nuclei were stained with 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI; 2ng/ml; Molecular Probes). Sections were mounted using ProLong Gold anti-fade reagent (ThermoFisher) ...
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bioRxiv - Genomics 2023Quote: ... slides were stained in 300 nM 4′,6-diamidino-2-phenylindole (DAPI, ThermoFisher Scientific, D1306) and mounted in ProLong Diamond antifade mountant (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Nuclei were counterstained with 4’,6-diamino-2-phenylindole (Prolong Gold antifade reagent DAPI; Invitrogen). The primary antibodies were vimentin ...
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bioRxiv - Cell Biology 2024Quote: After mounting the cells using ProLong Diamond with 4’,6’-diamidino-2-phenylindole (DAPI) (Invitrogen), they were imaged using a DeltaVision II microscope system with an Olympus Ix-71 inverted microscope ...
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bioRxiv - Immunology 2024Quote: ... Slides were washed and stained for 5min with 4′,6-diamidino-2-phenylindole (DAPI, ThermoFisher) for detection of nuclei and mounted with Fluoromount-G (SouthernBiotech) ...
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bioRxiv - Immunology 2024Quote: ... as well as live/dead cells using 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher). In the case of intracellular stainings ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 μl cDNA (1000 ng/ 100 μl) and 6 μl nuclease-free water (Thermo Scientific). qRT-PCR cycles included pre-incubation at 95°C for 5 minutes followed by 40 cycles of 95°C for 10 seconds 60°C for 30 seconds and 72°C for 30 seconds ...
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bioRxiv - Bioengineering 2024Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, Cat # N13195) and incubated for 30 minutes in their respective culture conditions ...
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bioRxiv - Biophysics 2021Quote: ... Secondary antibodies were goat anti-mouse-Star red (Abberior, 2-0002-011-2) and goat anti-rabbit Star 580 (Abberior 2-0012-0050-8) (Life Technologies, 1:100).
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bioRxiv - Microbiology 2021Quote: ... a sequencing reaction was performed with 2 pmol of unmodified RNA and 2 µl of a 2 mM AS primer 1 labeled with Ned (Life Technologies SAS, France). Reverse transcription was performed as for the SHAPE (+ ...
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bioRxiv - Neuroscience 2022Quote: ... 100 μM 2-mercaptoethanol (2-ME, Gibco) and 1 × Antibiotic-Antimycotic (Gibco ...
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bioRxiv - Molecular Biology 2019Quote: ... the cells were washed three times in PBS and incubated in secondary antibody (2 mg/mL Alexa Fluor 488, Invitrogen, 1:1500, 2 mg/mL Alexa Fluor 594, Invitrogen, 1:1500; 2 mg/mL Alexa Fluor 568, Invitrogen, 1:1000) diluted in PBS for 1.5 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... minced into 1-2 mm squares and digested with Collagenase Type I (2 mg/ml, Gibco) at 37°C for 1 hour with agitation ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were split at 1:2 every 2 days using Trypsin-EDTA (ThermoFisher Scientific, Waltham, MA).
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bioRxiv - Cell Biology 2019Quote: ... minced into 1-2 mm squares and digested with Collagenase Type I (2 mg/ml, Gibco) at 37°C for 2 h with occasional agitation ...
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bioRxiv - Physiology 2023Quote: ... cardiomyocytes were incubated with 1 µM Fura-2- acetoxymethyl ester (Fura-2 AM, cell permeant, ThermoFisher) and 0.075% Pluronic F-127 (20% in DMSO ...
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bioRxiv - Cell Biology 2019Quote: ... 2×2 binning (3.7mm^2 field area)) on the ArrayScan VTI (Thermo Scientific) and analyzed simultaneously for percentage of GFP positive cells using the Target Activation Bioapplication (Thermo Scientific ...
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bioRxiv - Immunology 2020Quote: ... Serial frozen sections (6 μm in thickness) were cut on a cryostat and counterstained with DAPI (4′,6-Diamidine-2′-phenylindole; Thermo Fisher Scientific). The number of beads in the SED from 3-4 sections of two Peyer’s Patches per mouse (n=3–4 mice/group ...
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bioRxiv - Neuroscience 2019Quote: ... cortical cultures were loaded with fluo-4 AM (3 μM) or fura-2 AM (3 μM) plus 0.1% Pluronic F-127 (ThermoFisher) in a HEPES-buffered saline solution (HCSS ...
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bioRxiv - Biochemistry 2021Quote: ... or with 0.5 µg/ml doxycycline for 2-3 days) were combined in a 1:1 ratio and loaded with 5 µg/ml Indo-1 (Molecular Probes, AAT Bioquest, Sunnyvale, USA) and 0.04% of pluronic F-127 (AAT Bioquest ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated in 2-(N-(7-nitrobenz-2-oxa-1,3-diaxol-4-yl) amino)-2-deoxyglucose (2-NBDG) (Invitrogen) (20 µM ...
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bioRxiv - Physiology 2021Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher Scientific, USA) was dissolved in saline solution at 5 mg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... (2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) (100 μM; ThermoFisher Scientific) with Hoechst (1 μg/mL ...
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bioRxiv - Physiology 2019Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher Scientific, USA) was dissolved in PBS at 5 mg/ml ...
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bioRxiv - Immunology 2020Quote: ... Glucose uptake assay was performed by incubating cells with 50 µM 2-NBDG (2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose) (Invitrogen) for 90 min at 37°C prior to cell staining ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in glucose-free media containing 150 μM 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG Thermofisher) and incubated for 45 minutes at 37°C.
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bioRxiv - Microbiology 2023Quote: ... The cell lysate was mixed with 1 vol of lysis buffer B supplemented with 2% BSA and 50 µl of Dynabeads M-270 Streptavidin (Thermo Fisher Scientific, cat 65305) and incubated at 4°C for 30 min with rotation ...
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bioRxiv - Bioengineering 2023Quote: ... pRSV-Rev = 5: 3: 2) were co-transfected into HEK-293T cells at a ratio of 1:1 using lipofectamine 3000 (Invitrogen, USA, Cat: #L3000015). After 48 h ...
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bioRxiv - Neuroscience 2023Quote: ... An imaging window was constructed from three layers of microscope cover glass (1 x 5 mm, 2 x 3 mm diameter, Fisher Scientific, no. 1) joined with a UV-curable optical glue (NOR-61 ...
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bioRxiv - Plant Biology 2021Quote: ... two SlP4H3 OEX lines (#1, #2) and three SlP4H3 RNAi lines (#1, #6 and #7) by using the TRI reagent (Thermo Fisher Scientific, Waltham, MA, USA). Reverse transcription of approximately 1 μg of total RNA was performed with Superscript II® Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... Alkaline phosphatase staining was performed using the one-step nitro-blue tetrazolium (NBT) and 5-bromo-4-chloro-3’-indolyphosphate p-toluidine salt (BCIP) solution (Thermofisher).
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bioRxiv - Microbiology 2021Quote: ... The 5’ and 3’ fragments (2 μg of each) were ligated for 1 h at 16°C by T4 DNA ligase (Invitrogen) and purified by phenol-chloroform extraction ...
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bioRxiv - Biophysics 2021Quote: ... slides were rinsed in PBS for 3 x 5 mins and stained with 1 %g/mL 4,6-diamino-2-phenylindole (DAPI - Molecular Probes) for 3 mins in the dark at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... ionomycin (1 μg/ml) and monensin (2 μg/ml) for 3-4 hours at 37°C in complete IMDM medium (Gibco). Viability staining was performed using the fixable viability dye eFluor780 ...
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bioRxiv - Immunology 2022Quote: ... The sorted cells were then incubated with Totalseq-C antibodies (1:50 dilution) on ice for 30 min, washed 3 times (by default, top up with 2% FBS (Gibco) in DPBS (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... The next day, the larvae were washed (3×, 1× PBST) and incubated for 30 minutes in 2 μg/mL Hoechst 33342 (Invitrogen) in 1× PBT at room temperature ...
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bioRxiv - Immunology 2022Quote: ... The dissociated cells were washed 3 times with chilled PBS containing 1% heat-inactivated FBS and 2 mM EDTA (Gibco).
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bioRxiv - Immunology 2023Quote: ... Purified T cells were activated for 1 to 3 days with Human T-Activator anti-CD3/CD28 dynabeads at a 1:2 bead-to-cell ratio (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... the membrane was incubated in 10 mL of 3% BSA/TBST (w/v) with 2 µL streptavidin-HRP (1:5,000; Invitrogen, s911) for 1 hour at room temperature ...