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Citations for Thermo Fisher :
1201 - 1250 of 10000+ citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The lentivirus vectors were produced in HEK293FT cells by the co-transfection of plasmids pMD2.G and pCMVR8.74 with the pLKO.1-sh PKM2 using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA). To produce H1299 shPKM2 cells ...
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bioRxiv - Synthetic Biology 2024Quote: A National Institute of Standards and Technology traceable standard (Invitrogen, catalog no. F36915) was used to convert arbitrary fluorescence measurements to micromolar equivalent fluorescein (MEF) ...
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bioRxiv - Developmental Biology 2021Quote: ... Nuclei were collected (900 g, 10 min) and resuspended in 500μl staining buffer (nuclease free PBS (1X, Thermo Fisher Scientific), BSA (0.5% wt/vol ...
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bioRxiv - Microbiology 2022Quote: ... A pellet was obtained using a short spin (250 × g for 5 min) and was resuspended in serum-free RPMI 1640 medium (Gibco) containing antibiotics (Pen/Strep ...
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bioRxiv - Biochemistry 2024Quote: ... 10 μg/mL DiI-LDL4 in the presence of PBS or the various unlabelled APOA1 proteins or rHDLAPOA1 (of density 1.1 g/mL) in serum-free RPMI (Gibco, 1640) were incubated with HCAECs for 20 min at 37°C to allow for internalization ...
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bioRxiv - Bioengineering 2022Quote: ... Mixed populations were then centrifuged at 100 g for 5 minutes and resuspended in serum-free RPMI medium (Gibco®) with magnetic nanoparticles at [Fe] = 50 mM and supplemented with citrate at 5 mM ...
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bioRxiv - Developmental Biology 2023Quote: ... and aliquoted to 1 µg/µL in RNAse-free water at -80 °C for storage (concentration was determined using a Qubit 3 Fluorometer, Invitrogen).
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bioRxiv - Neuroscience 2021Quote: ... Co-IP was performed with Pierce Crosslink Magnetic IP/Co-IP kit (#88805, Thermo Fisher) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Co-IP was performed with Pierce Classic Magnetic IP/Co-IP kit (#88804, Thermo Fisher) according to the instructions of manufacturer ...
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bioRxiv - Genomics 2020Quote: ... Trono) and 13.6 µg target library plasmid were mixed in 651 µL Opti-MEM (serum free, Opti-MEM (Thermo Fisher Scientific)) ...
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bioRxiv - Cancer Biology 2023Quote: Synthesis of cDNA was performed on 1 µg of DNAse-free RNA using the SuperScript VILO cDNA Synthesis Kit (Invitrogen, Thermo Fisher). Power SYBR Green PCR Master Mix (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... 10 µg of raw RNA was digested with the Invitrogen Turbo DNAse free kit (Invitrogen/Thermo Fisher Scientific, Waltham, MA, U.S.A.). The Bioanalyzer 2100 (Agilent ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: HBV DNA was extracted from 5 μL of serum using the SMITEST EX-R&D Nucleic Acid Extraction Kit (Medical & Biological Laboratories, CO. LTD, Nagoya, Japan) and dissolved in 20 μL nuclease-free water (Thermo Fisher Scientific Inc., Waltham, MA). Real-time PCR was used to measure the serum HBV DNA concentration using the TaqMan Fast Advanced Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... Post sonication and centrifugation purification was done on the Cobalt resin affinity chromatography column (ThermoFisher Scientific, Waltham, MA, USA). After column saturation overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 20,000 rpm 30 minutes) and the supernatant was incubated and passed over a HisPurTM Cobalt Resin (ThermoFisher Scientific 89965) in gravity columns ...
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bioRxiv - Biochemistry 2021Quote: ... The recombinant proteins were affinity purified by overnight binding to HisPur Cobalt Resin (Thermo Fisher Scientific, U. S. A.) in buffer A (50 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Microbiology 2020Quote: The remainder of the protein was incubated with 750 µl of HisPurTM Cobalt Resin (Thermo Scientific Pierce, Rockford, IL) on a rocker for 45 min at room temperature ...
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bioRxiv - Synthetic Biology 2022Quote: ... Lysates were supplemented with 10 mM imidazole and 600 μl was applied to HisPur Cobalt resin (300 μl, ThermoFisher) equilibrated in Binding Buffer (T50N300+pi ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant proteins were purified by immobilized metal affinity chromatography (IMAC) as previously described30 using HisPur cobalt resin (Thermo Scientific). Before elution ...
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bioRxiv - Microbiology 2024Quote: Erythrocyte pull-down studies were performed with 6His-tagged EtpA linked to cobalt-coated magnetic beads (Dynabeads, Invitrogen 10103D) and blood group A1 red blood cells (RBC ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 200 µl of permeabilization buffer with 10 µg/ml donkey anti-mouse immunoglobulin G–AlexaFluor568 (Thermo Fisher Scientific; Cat. No. A10037), donkey anti-rabbit immunoglobulin G–AlexaFluor647 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... 0.5-1.0 mg of cell lysate was incubated with 2-4 µg of antibody for 1 h followed by incubation with 8-10 µl of Protein G agarose (Thermo Fisher) for another 30-45 min ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 µg of an HA-tag antibody (MBL, M132-3) was mixed with 20 µL of Dynabeads Protein G (Invitrogen, #10004D) and subsequently added to extracts ...
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bioRxiv - Neuroscience 2022Quote: ... placed into 1.5 ml tubes and incubated with recombinant sNEGR1 (10 µg/ml) or bovine serum albumin (10 µg/ml) diluted in Neurobasal A supplemented with gentamycin/amphotericin (1:500, ThermoFisher Scientific) for 24 h at room temperature with constant agitation ...
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bioRxiv - Cell Biology 2024Quote: ... A total of 1 µg of sgRNA containing px459 vectors was mixed with a final concentration of 0.04 µg/ml Lipofectamine 2000 (Thermo Fisher Scientific) in Opti-MEM (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... the volume of each tube was adjusted to 50 mL and the eggs were treated with an antibiotic cocktail for 2 hours with gentle agitation at 10 rev/min: penicillin G: streptomycin at 100 µg/mL (GIBCO 15140148), kanamycin sulfate at 400 µg/mL (PAN BIOTECH P06-04010P ...
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bioRxiv - Cell Biology 2023Quote: ... 0.25 µg HA-PPM1H constructs and 6.25 µg PEI were diluted in 200 µL Opti-MEM™ Reduced serum medium (Gibco™) per well ...
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bioRxiv - Cell Biology 2023Quote: ... or 0.5 µg rtTA-puro and 1 µg of PiggyBac (for control rtTA cells) in 100 µl Opti-MEM solution (31985062, Life Technologies) containing 5 µl of X-tremeGENE 9 transfection reagent (XTG9-RO Roche ...
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bioRxiv - Cell Biology 2024Quote: ... Sample protein content was determined with the Pierce detection assay and 200-800 µg of proteins were loaded on Dynabeads Protein G for Immunoprecipitation (ThermoFisher Scientific); the beads were previously incubated for 10 min on a wheel at room temperature with PBS-Tween-20 0.01% supplemented or not with 2.5-5 µg of MIC60 ...
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bioRxiv - Biochemistry 2024Quote: ... Parasites were cultured in HMI-9 medium supplemented with 10% inactivated FBS and 2.5 µg ml-1 G-418 (Life Technologies Europe) at 37°C in a humidified atmosphere containing 5% CO2 (G-418 was included for maintenance of TetR and T7RNAP) ...
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bioRxiv - Neuroscience 2024Quote: ... Approximately 0.3-1 mg of cleared lysate was immunoprecipitated for 1 h at 4°C with 3-4 µg of antibody as indicated before the addition of 60 µl of 50% Protein G Agarose (Cat#15920010, Thermo Fisher) and incubation for 1 h at room temperature with agitation ...
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bioRxiv - Cell Biology 2024Quote: ... 20 µg for a 10 cm petri dish and 50 µg for a 15 cm petri dish) using Lipofectamine 3000 (Invitrogen, L3000015) or polyethylenimine (Polysciences ...
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bioRxiv - Cell Biology 2024Quote: ... Lysates were mixed with 5 µg antibody and incubated at 4°C for overnight followed by incubation with Dynabeads protein G (Life Technologies) for 4 h at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Protein G Dynabeads (G 10004D, Invitrogen) were coated with antibodies indicated on the figures according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... Organic acids were separated using an IonPac AS11-HC (2 mm, Thermo Scientific) column connected to an ICS-5000 system (Thermo Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... The materials used for the solvents were: GC-grade Tributylamine 99% (ACROS ORGANICS), LC/MS grade acetic acid Optima (Fisher Chemical) ...
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bioRxiv - Microbiology 2021Quote: ... Solvents were purchased from the following vendors: GC-grade Tributylamine 99% (ACROS ORGANICS), LC/MS grade acetic acid Optima (Fisher Chemical) ...
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bioRxiv - Neuroscience 2021Quote: ... then switched to 30% sucrose with 5% sodium azide (ACROS Organics 19038-1000) and stored at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The organic fraction was evaporated using a Savant Speedvac spd111v (Thermo Fisher Scientific) at room temperature and the remaining lipid pellet was stored at −20°C under argon ...
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bioRxiv - Systems Biology 2020Quote: ... RFP was induced with 20 nM anhydrotetracycline hydrochloride (233131000, Acros Organics, Geel, Belgium). GFP was expressed from prplN-GFP48 ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue was extracted and flash frozen in 2-methylbutane (Acros Organics, NJ, USA) chilled in a bath of dry ice with 100% ethanol (∼−70°C) ...
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bioRxiv - Bioengineering 2021Quote: ... Organic solvents were removed by vacuum drying overnight in a Savant SpeedVac (ThermoFisher). The dried SLs were stored in a -20 freezer until analysis.
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bioRxiv - Pathology 2021Quote: ... Solvents were purchased from the following vendors: GC-grade Tributylamine 99% (ACROS ORGANICS), LC/MS grade acetic acid Optima (Fisher Chemical) ...
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bioRxiv - Plant Biology 2020Quote: ... Cell walls were stained with 0.5mg mL-1 propidium iodide (PI, Acros Organics) dissolved in water for 5 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... Solvents were purchased from the following vendors: GC-grade tributylamine 99% (ACROS ORGANICS), LC/MS grade acetic acid Optima (Fisher Chemical) ...
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bioRxiv - Cell Biology 2022Quote: ... The organic fraction was evaporated using a Savant Speedvac spd111v (Thermo Fisher Scientific). Lipid pellets were reconstituted in running solution (CH3OH:CHCl3:NH4OH ...
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bioRxiv - Plant Biology 2022Quote: ... stained for cell walls with 0.05% toluidine blue or ruthenium red (Acros Organics) for 10 min and then rinsed with water to wash off excess dyes ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were blocked in PBS containing 3% Bovine Serum Albumin (BSA; Acros Organics) for 30 min before incubated with Alexa-647 for 1h at RT ...
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bioRxiv - Microbiology 2021Quote: ... glucose (Alpha Biosciences) 0.5% (w/v) and 50µm microcrystalline cellulose (Acros Organics, Belgium) 1% (w/v ...
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bioRxiv - Biochemistry 2021Quote: ... and 5-aminolevulinic acid hydrochloride were purchased from Acros Organics (Fair Lawn, NJ). The 11-hydroxylauric and 12-hydroxylauric-d20 acid standards were purchased from Santa Cruz Biotechnology ...