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Citations for Thermo Fisher :
1051 - 1100 of 10000+ citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: Co-immunoprecipitation was performed using Dynabeads Co-Immunoprecipitation Kit (ThermoFisher Scientific, CAT# 14321D) following the protocol supplied by the manufacturer ...
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bioRxiv - Molecular Biology 2024Quote: Co-immunoprecipitation was performed by using Dynabeads® Co-Immunoprecipitation kit (Invitrogen, USA) and following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Co-IP experiments were performed using PierceTM Co-Immunoprecipitation kit (ThermoFisher Scientific, 26149) modified to use Dynabeads Protein G (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates were cleared by centrifugation at 21130 g for 10 min at 4°C and quantified for protein concentration using an albumin standard (Thermo Fisher 23209) and Bradford reagent (Bio-Rad 5000006) ...
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bioRxiv - Bioengineering 2024Quote: ... SMR frequency peaks were calibrated (hertz to picogram) with 8 μm polystyrene beads with a known density of 1.05 g/mL (Thermo Fisher, Duke Standards). Calibration calculations for both cell volume and buoyant mass were conducted using MATLAB.
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bioRxiv - Microbiology 2024Quote: ... 1% penicillin/streptomycin (PS, 5000 U.mL−1 and 5000 μg mL−1 respectively; Life Technologies), 1% L-Glutamine (Gln ...
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bioRxiv - Neuroscience 2023Quote: ... an SDS Bis-Acrylamide gel was loaded with 20 µg of protein from each sample and a standard protein marker (PageRuler Plus, cat. No. 11852124; Thermo Fisher Scientific, USA). The proteins were transferred to FL-PVDF membranes (cat ...
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bioRxiv - Pathology 2020Quote: ... Phoenix cells were grown in DMEM containing 10% FBS and co-transfected by pLV-mCherry and pcDNA-Spike or pMD2.G vector using a calcium phosphate kit (ThermoFisher, Waltham, MA, USA). The supernatant with produced virus (Spp or VSV-G lentivirus ...
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bioRxiv - Microbiology 2024Quote: ... The bacteria-macrophage co-culture plate was centrifuged at 500 x g for 3 minutes with a Multifuge X1R Refrigerated Centrifuge (Thermo Scientific, Waltham, MA) to synchronize phagocytosis of the bacteria ...
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bioRxiv - Molecular Biology 2022Quote: ... JPH2 or JCN and their interacting proteins were co-precipitated by using an immunoprecipitation kit (Dynabeads Protein G; Life Technologies, Inc., ON, Canada) in tissue or cell lysates according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Live imaging medium for cell lines consisted of phenol red-free DMEM (4.5 g l-1 glucose, L-glutamine, and 25 mM HEPES, #21063-029, Invitrogen) supplemented with 10% FBS ...
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bioRxiv - Bioengineering 2022Quote: ... Imaging medium consisted of phenol-free DMEM (4.5 g l-1 glucose, L-glutamine, and 25 mM HEPES, 21063-029, Invitrogen) supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2020Quote: ... Beas-2B cells were cultured at 37°C in a humidified atmosphere of 5% in serum-free 1× defined keratinocyte SFM Gibco) supplemented with 5 µg/mL gentamicin (Gibco). The medium was replaced every 2–3 days and cells were passaged every 4–5 days ...
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bioRxiv - Bioengineering 2021Quote: ... Stable UC-MSC-DN cell lines expressing a single nuclease domain were generated by nucleofection of 1 µg of an endotoxin-free expression vector into 105 UC-MSCs using the Neon Transfection system (Invitrogen) in the Neon Tip10 with the following parameters of a single electric pulse ...
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bioRxiv - Molecular Biology 2021Quote: ... aliquots of 10 x 104 uninfected macrophages suspended in 10 mL SFB-free DMEM were incubated with: i) 5 µg of lipofection pBlueBacHis2/CAT plasmid (Invitrogen); ii ...
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bioRxiv - Cell Biology 2022Quote: ... 4T1 cells were transduced with cell-free virus-containing supernatants for 48 hours and selected with 100 µg/mL of neomycin (Gibco) for 96 hours.
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bioRxiv - Neuroscience 2022Quote: ... Reverse transcription of polyA mRNA from 5 µg total DNA-free RNA was performed using Superscript First Strand Synthesis (Invitrogen) with Oligo-dT primers ...
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bioRxiv - Molecular Biology 2023Quote: ... 2.5 µg of RNA was treated with 1 unit of Turbo DNase using the TURBO DNA-free Kit (AM1907, Invitrogen) and incubated for 30 min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... RWPE-1 cells were cultured in keratinocyte serum-free media supplemented with 25 mg of bovine pituitary extract and 2.5 µg epidermal growth factor (ThermoFisher, 17005042). All cells were maintained at 37°C at 5% CO2 for <15 passages ...
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bioRxiv - Biochemistry 2023Quote: ... Residual DNA was removed from RNA samples (1 µg of total RNA input) using the DNA Free kit (Ambion Inc.) before cDNA synthesis using the iScript cDNA synthesis kit (Bio-Rad) ...
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bioRxiv - Bioengineering 2024Quote: ... Standard curves were constructed using serial dilutions of λDNA standard (Invitrogen, 25250) in a 10 mM Tris-HCl solution (pH 7.5) ...
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bioRxiv - Plant Biology 2023Quote: ... Instrument calibration was performed with a BSA standard (Thermo Scientific Albumin Standard). 20 μM 14-3-3ω in a volume of 50 μl was separated on a Superdex 200 increase 10/300 GL column (Cytiva Life Sciences ...
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bioRxiv - Genomics 2020Quote: RNA from spermatozoa pellets was extracted using a standard Trizol® approach and treated with TURBO DNA-free™ Kit (Invitrogen; Carlsbad, USA) (Gòdia et al. ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was purified using HisPur™ cobalt superflow agarose (0.5 ml) (Thermo Fisher Scientific™, Waltham, USA), see above ...
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bioRxiv - Biochemistry 2021Quote: ... 10 mM imidazole and 10% (v/v) glycerol) and incubated with pre-equilibrated HisPur Cobalt resin (ThermoFisher Scientific) at 4°C for 2 h ...
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bioRxiv - Biochemistry 2022Quote: ... Histidine rich glycoprotein (HRG) was purified from human plasma with a cobalt-loaded resin (Thermo Scientific, Waltham, America) using IMAC-based enrichment (56) ...
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bioRxiv - Biochemistry 2023Quote: ... This solution was incubated for 1h at ~7°C with 0.25 ml of HisPur Cobalt resin (Thermo Scientific). The beads were allowed to settle to the bottom of a protein purification column and washed 5 times with 10 ml of washing buffer comprising 10 mM imidazole and 0.1% Triton X-100 in 2xPBS ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant obtained was then loaded onto cobalt resin affinity-chromatography column (Thermo Fisher Scientific, Waltham, MA, USA), equilibrated with pH 7.4 wash buffer containing 50 mM sodium phosphate and 300 mM NaCl ...
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bioRxiv - Cell Biology 2024Quote: ... clarified cell supernatant was supplemented with 10mM immidazole and then incubated with HisPur cobalt resin (Thermo Fisher Scientific) for 40 min at 4 °C and then washed with 20x resin bed volume of binding buffer supplemented with 20mM immidazole ...
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bioRxiv - Molecular Biology 2020Quote: ... following standard procedures (Invitrogen). To generate the transgenic Sg4_CD cell line ...
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bioRxiv - Neuroscience 2024Quote: ... and standard PBS (Gibco) in the ratio 1:1:48 with final concentration of FIPI 0.5 mg/ml ...
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bioRxiv - Biochemistry 2022Quote: ... expression clone cDNA or control cDNA (1 µg) was mixed with packaging plasmids MD2G (1 µg) and PSPAX2 (1 ug) in 600 µL per plate OPTIMEM (Gibco) and Lipofectamine™ 2000 transfection reagent (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... 72 µg PE-Puro or PE-FeLV plasmid were mixed with 8 µg pCS2-GFP and 40 µl Lipofectamine P3000 (Invitrogen) in 3.2 ml Opti-Mem (Gibco) ...
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bioRxiv - Molecular Biology 2023Quote: ... and supernatants were incubated (2 h, 4°C) with 5 µg antibodies conjugated to 25 µL protein G dynabeads (Invitrogen). Samples were immobilised ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatant proteins were then quantified using the BCA assay and equivalent protein concentrations (800 µg) were immunoprecipitated with DynabeadsTM Protein G (Invitrogen) coupled to a mouse anti-biotin primary antibody at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... whole cell or nuclear extracts were incubated with 2.5 µg of anti-FLAG antibody bound to 50 µl Dynabeads Protein G (Invitrogen #10003D) overnight at 4°C with gentle rotation ...
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bioRxiv - Physiology 2023Quote: ... Lysates were spun down at 18,000 g for 10 minutes and normalised to 700 µg of protein as determined with BCA (ThermoFisher Scientific). 1.5ul of mouse anti-FLAG M2 (Sigma #F1804 ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was incubated o/n at with rotation at 4°C with 2 µg IP antibody and for 1 h with Dynabeads Protein G (Invitrogen). Alternatively ...
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bioRxiv - Biochemistry 2023Quote: ... in TSMBB or 5 µg/ml anti-mannose antibody (100-4G11-A) (50) followed by 2 µg/ml anti-mouse IgM AF 647 (Invitrogen); (iii ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatant proteins were then quantified using the BCA assay and equivalent protein concentrations (800 µg) were immunoprecipitated with DynabeadsTM Protein G (Invitrogen) coupled to a primary antibody at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... and envelope (pMD2.G; 0.5 µg) plasmids using either FuGENE 6 or Lipofectamine 3000 transfection reagent (both from Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: The sonicated samples (approximately 5 µg chromatin/sample) were cleared for 2 hours using non-coupled magnetic beads (Protein G Dynabeads, Invitrogen), then further mixed with 5 µL antibody (anti-H3K27me3 ...
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bioRxiv - Neuroscience 2023Quote: ... transfected cell lysates (500 µg total protein in 200 µl) were pre-cleared by incubating with 10 µl Dynabeads protein G (Invitrogen) at 4°C for 1 hr ...
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bioRxiv - Molecular Biology 2024Quote: ... 1.65×106 FL3 cells were seeded in a P10 dish and transfected 24 hrs later with 12 µg total DNA (2 μg Twin-Strep-Tag-RBP expression vector and 10 µg pcDNA3 PL) using Lipofectamine 2000 (Invitrogen) according to manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: ... was injected at a concentration of 100 µg Cadaverine/20 g of mice and lysine-fixable 3 kDa dextran conjugated to tetramethylrhodamine (D3308, Invitrogen) at a concentration of 250 µg dextran/20 g ...
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bioRxiv - Microbiology 2024Quote: ... 12 µg replicon bacmid DNA and 4 µg spike or VSV-G plasmid were mixed well in 500 µl serum reduced OPTI-MEM (Invitrogen). Simultaneously 48 µl lipofectamine 2000 was diluted in 500 µl serum reduced OPTI-MEM ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclear extracts were quantified with Bradford and 2mg of lysate was mixed with 6 µg of antibody and 50 µL of Protein G magnetic beads (Dynabeads, Invitrogen) in 500 µl of buffer C (300 mM NaCl ...
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bioRxiv - Synthetic Biology 2021Quote: ... and adjusted to pH 7.5 using 3M Acetic Acid (ACROS Organics, #423225000). This was then diluted to 1X and supplemented with DNA template ...
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bioRxiv - Biophysics 2020Quote: ... Liposomes were fluorescently labeled by incubating them with Nile Red (Acros Organics) in MeOH at a 1:100 molar ratio (dye:lipid ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... All other chemical reagents were purchased from Acros Organics (Morris Plains, NJ). All ACS reagent grade EMD brand solvents were purchases from VWR International (Radnor ...