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Citations for Thermo Fisher :
1201 - 1250 of 10000+ citations for 6 IODO BENZO D 1 3 OXAZIN 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Mixed protein eluates were separated by one-dimensional gel electrophoresis on a 4–12% NuPage Novex Bis–Tris gel (Invitrogen), stained with the Colloidal Blue Staining Kit (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... One to 20 μg of total protein were separated by NuPAGE™ 4-12% Bis-Tris Gel (Thermo Fisher Scientific) and transferred onto PVDF membranes (Bio-Rad ...
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bioRxiv - Genomics 2020Quote: ... Abl.3 and Abl.4 (13) were cultured in Roswell Park Memorial Institute medium (Gibco), containing 15% FBS (Sigma) ...
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Potassium channel-driven bioelectric signaling regulates metastasis in triple-negative breast cancerbioRxiv - Cancer Biology 2021Quote: ... CDH11 #4: 5’-CCUUAUGACUCCAUUCAAA-3’ using Lipofectamine RNAiMAX transfection reagent (13778030; ThermoFisher Scientific, Waltham, MA) in serum-free DMEM ...
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bioRxiv - Cell Biology 2021Quote: HAEC cells (passage 3-4) were lysed in ice-cold RIPA buffer (ThermoFisher, cat# 89900) containing protease and phosphatase inhibitors (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell number was determined every 3-4 days using the Countess automated cell counter (Invitrogen). 20,000 cells were then re-plated with fresh media and compound ...
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bioRxiv - Neuroscience 2019Quote: ... Lines were passaged every 3-4 days using enzymatic detachment with Stempro Accutase (ThermoFisher; A1110501) for 5 minutes and re-plated in mTeSR1 medium with 10µM Rock Inhibitor (Y2732 ...
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bioRxiv - Biochemistry 2021Quote: ... RNA was isolated from approximately 3-4 x 107 cells using TRIzol reagent (Thermo Fisher), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... for 3-4 hr in the presence of brefeldin A (BFA; 10ug/mL; Life Technologies).
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bioRxiv - Cancer Biology 2024Quote: ... 3-4 μm paraffin sections were prepared with a HM 355S microtome (Fisher Scientific, 10862110), deparaffinized and rehydrated up to 96% ethanol (v/v) ...
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bioRxiv - Immunology 2023Quote: ... Cell passaging was performed every 3 to 4 days using 0.05% Trypsin-EDTA solution (Gibco). Expi293F cells were maintained in Expi293 Expression Medium (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: Hippocampal neurons were transfected at day in vitro (DIV)3-4 using Lipofectamine 2000 (Invitrogen). Shortly ...
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Migration and establishment of progenitor pool of melanocytes is governed by SEMA3E-PLXND1 signalingbioRxiv - Developmental Biology 2023Quote: ... cells were switched to M254 medium for 3-4 population doublings (Thermofisher Scientific, Life Technologies).
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Migration and establishment of progenitor pool of melanocytes is governed by SEMA3E-PLXND1 signalingbioRxiv - Developmental Biology 2023Quote: ... cells were switched to M254 medium for 3-4 population doublings (Thermofisher Scientific, Life Technologies).
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bioRxiv - Cell Biology 2023Quote: ... followed by a second round of transfection 3-4 hours later using RNAiMax (Life Technologies) according to the manufacturer’s instructions with final siRNA concentration of 50 nM and 20 nM for Sac2 and OSBP ...
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bioRxiv - Cancer Biology 2023Quote: ... for 3-4 days before they were collected directly in TRIzol reagent (Invitrogen cat#15596026). Before collection ...
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bioRxiv - Microbiology 2020Quote: ... and resuspended in 0.1 ml of PBS supplemented with the lipophilic styryl membrane dye N-(3-triethylammoniumprpyl)-4-(p-diethylaminophenyl-hexatrienyl) pyridinium dibromide (FM4-64, Molecular Probes, Invitrogen; 10 μg.ml-1) (Pogliano et al. ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cell layers are examined for cell morphology using a phase contrast microscope washed with media and then 10 uM of 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in the presence or absence of 100 nM insulin as the initiating step and incubated for 20 or 30 minutes ...
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bioRxiv - Systems Biology 2023Quote: ... 1 vein graft slide containing 2-4 cross sections was double stained with the general macrophage marker Mac-3 (rat anti-mouse, Fisher Scientific, B550292, 1:600) and either iNOS (rabbit anti-mouse ...
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bioRxiv - Neuroscience 2024Quote: ... and diluted secondary antibodies were applied at 4 °C for approximately 3 hours using Alexa Fluor 488 goat anti-rabbit (1:1000; Invitrogen, Waltham, MA; Catalog #A11008); Alexa Fluor Plus 555 goat anti-rat (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... using the NanoDrop One/OneC spectrophotometer (ThermoFisher, Cat. No. ND-ONE-W) to determine gDNA concentrations ...
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bioRxiv - Cell Biology 2022Quote: ... 250.000 HEK293T cells were seeded per p35 plate (pre-coated Corning BioCoat Poly-D-Lysine 35 mm #354467 or uncoated Nunclon multidish 6 well plates, Thermo Scientific #140685 coated with 50 µg/ml Poly-D-Lysine solution (Gibco A38904-01 ...
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bioRxiv - Microbiology 2022Quote: ... The resulting PCR product was digested with BsiWI and ligated in frame with a N-terminal 3 x FLAG-tag and a C-terminal 6 × histidine tag into a modified and BsiWI-digested pET101/D-TOPO expression vector (Thermo Scientific), yielding pET101-NanH1.
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bioRxiv - Cancer Biology 2024Quote: ... neurons were plated at a density of 0.8x106 cells per well of 6-well plates coated with 50 ug/mL Poly-D-Lysine (Thermofisher, #A38904-01) and 1ug/mL Laminin (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... Synthetic cDNA encoding a self-cleaving polyprotein Bcl-6.t2A.Blc-xL to express the germinal center B cell-associated transcription factors Bcl-6 and Bcl-xL was synthesized (B6L; Integrated DNA Technologies) and cloned in pENTR/D-TOPO (Thermo Fisher), generating pENTR.B6L (35 ...
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bioRxiv - Cell Biology 2023Quote: ... on non-tissue-culture treated 6-well plates that were pre-treated overnight with PBS + 0.1 mg/ml Poly-D-lysine (Thermo Scientific, A3890401) and 200 μg/ml human JAGGED1-Fc ...
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bioRxiv - Microbiology 2022Quote: ... PAFB was labelled with the green fluorophore 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionyl ethylenediamine hydrochloride (BODIPY™ FL EDA, Invitrogen, Waltham, MA, USA) as described (32).
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bioRxiv - Cell Biology 2019Quote: Mouse antibodies against HuC/D (Molecular Probes #A21271; 1:500), β-Catenin (Sigma #C7207 ...
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bioRxiv - Genetics 2021Quote: ... mouse monoclonal anti-HuC/D 1:500 (Invitrogen A-21271); mouse monoclonal Anti-Cre recombinase 1:250 (Millipore-Sigma MAB3120) ...
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bioRxiv - Bioengineering 2022Quote: ... and 1 mL 100mg/mL D(-)-arabinose (Fisher Scientific, NY) were added into growth media to provide selective pressure for maintaining the plasmid and expressing GFP.
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bioRxiv - Neuroscience 2020Quote: ... mouse α-HuC/D (1:250, Molecular Probes A-21271), rabbit α-GFP (1:2000 ...
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bioRxiv - Immunology 2022Quote: ... L/D-BV506 (1:200, Invitrogen, catalog #65-0866-14), hCD19-BV711 (1:100 ...
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bioRxiv - Molecular Biology 2022Quote: ... or HuC/D (1:100, A-21271, Thermo Fisher Scientific) overnight at 4□°C ...
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bioRxiv - Neuroscience 2022Quote: ... HuC/D (Mouse, Thermo Fisher Scientific, #A-21271, 1:400), PHOX2B (Goat ...
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bioRxiv - Neuroscience 2024Quote: ... and anti-HUC/D (pan-neuronal marker) (1:30) ( Invitrogen).
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bioRxiv - Microbiology 2023Quote: ... 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (Thermo Scientific), 100 μg/ml ampicillin and 50 μg/ml kanamycin after 1 day at 30°C ...
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bioRxiv - Microbiology 2023Quote: ... 1mM IPTG (Isopropyl β-d-1- thiogalactopyranoside, Thermo Fisher Scientific) was added to induce gene expression ...
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bioRxiv - Biochemistry 2023Quote: The panel of synthetic peptide standards and the products of immunoprecipitated heparan-sulfate 6-O-sulfotransferase 1/2/3 (H6ST-1/2/3) in vitro Tyr sulfation assays were analyzed using Proteome Discoverer 2.4 (Thermo Scientific) in conjunction with MASCOT 59 against either a custom database of all (12 ...
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bioRxiv - Bioengineering 2023Quote: ... KM-met released from CS-NP was quantified at 233 nm using a NanoDrop One microvolume UV-Vis spectrophotometer for up to 6 hours at room temperature (Thermofisher Scientific, Waltham, MA, USA). At the endpoint ...
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bioRxiv - Developmental Biology 2020Quote: ... One day after transfection of 1 μg of vector using Lipofectamine 2000 (Invitrogen), cells were treated with Dox (0.5 μg/ml ...
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bioRxiv - Biophysics 2021Quote: ... One microliter of the pellet was spread on an 1% agarose (#16500500, Invitrogen) pad (with no drug added) ...
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bioRxiv - Genomics 2022Quote: One lobe of lung was immersed in 1 ml Trizol solution (Invitrogen, 15596018) in Lysing Matrix A homogenization tubes (MP Biomedicals ...
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bioRxiv - Immunology 2022Quote: ... one-fourth of a mouse brain was placed in 1 mL Trizol (Ambion), mechanically homogenized using 1 mm zirconia/silica beads (Biospec ...
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bioRxiv - Microbiology 2022Quote: ... We loaded one plate with media containing 1 μg/mL BCECF (Thermo Fisher) to measure the environmental pH during growth ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mM TCEP) supplemented with one EDTA-free Pierce Protease Inhibitor Tablets (ThermoFisher). Lysed cells were then centrifuged for 30 min at 15000 rpm ...
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Tracer-based metabolomics for profiling nitric oxide metabolites in a 3D microvessel-on-a-chip modelbioRxiv - Cell Biology 2023Quote: ... followed by a one-hour incubation with Hoechst (1:2000; H3569, Invitrogen, USA), rhodamine phalloidin (1:200 ...
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bioRxiv - Biochemistry 2020Quote: ... HEK 293 cells were plated onto 6-well plates (2.5-4.5 × 105 cells per well) in 3 ml of MEM (Life Technologies) with 10% FBS (Life Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were incubated with lentiviral supernatant and 3 days later selection begun with 6 μg/mL puromycin (Thermo Fisher), which was maintained during all subsequent culturing.
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bioRxiv - Microbiology 2022Quote: ... and N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (biotin-X DHPE; Molecular Probes, Life Technologies). Planar bilayers were formed from 200-nm liposomes in channels of a PDMS flow-cell using vesicle spreading method (36) ...
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bioRxiv - Microbiology 2022Quote: ... and N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (biotin-X DHPE; Molecular Probes, Life Technologies). Planar bilayers were formed from 200-nm liposomes in channels of a PDMS flow-cell using vesicle spreading method (36) ...