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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for 6 IODO BENZO D 1 3 OXAZIN 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... (1:300; 4 h, RT, Invitrogen) were used as secondary antibodies ...
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bioRxiv - Bioengineering 2024Quote: ... OCT-4 (701756, Invitrogen, 1:1000), TRA-1-60 Podocalyxin (14-8863-82 ...
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bioRxiv - Developmental Biology 2021Quote: ... For experiments glass-bottomed imaging dishes were exposed to deep UV light for 6 minutes and coated with Poly-D-Lysine (ThermoFisher) for a minimum of 20 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... at room temperature in a holding chamber containing oxygenated imaging media (50% MEM, 50% HBSS, 6 mg/mL D-glucose, and 25 mM HEPES; all from Invitrogen/ThermoFisher ...
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bioRxiv - Plant Biology 2022Quote: ... and the amplified DNA fragment and an annealed linker oligo (for GFP: primers 5 and 6) were introduced into the pENTR/D-TOPO vector (Invitrogen; sequences were confirmed by Sanger sequencing using primers 7 and 8 ...
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bioRxiv - Cell Biology 2022Quote: ... dissociated neurons were resuspended and cultured at 37°C in a humidified incubator with 5% CO2 and 95% O2 in poly-D-lysine coated 6-well plates in neurobasal media (12349015, Gibco) supplemented with 1% GlutaMAX™ Supplement (35050-061 ...
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bioRxiv - Neuroscience 2021Quote: ... Longitudinal imaging of vascular leakage in C57BL/6 mouse was performed for 5 days after injecting 600 μg of 3kDa TRITC-dextran (LIF-D-3308, Invitrogen) and 600 μg of 2MDa FITC-dextran (LIF-D-7137 ...
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bioRxiv - Cell Biology 2021Quote: ... For experiments glass-bottomed imaging dishes were exposed to deep UV light for 6 minutes and coated with Poly-D-Lysine (ThermoFisher) for a minimum of 20 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthetized from 2ug of total RNA using MMLV-Reverse transcriptase and d(N)6 random primers in a 20ul reaction following the manufacturer’s protocol (ThermoFisher Scientific). RT-qPCR analyses were performed in triplicate using 2.5 μl of cDNA ...
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bioRxiv - Biochemistry 2024Quote: ... the cDNA fragments in pENTR-D-TOPO were transferred to the modified pHAGE plasmids (6) using LR recombination (Invitrogen 56484).
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bioRxiv - Neuroscience 2022Quote: ... We incubated overnight with the anti-GFP primary antibody at 4 degrees (1:1000, A-6455 Invitrogen, 0.1M PBS 0.3% tx100, 3% NGS). After abundant PBS washes ...
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bioRxiv - Genomics 2022Quote: ... Plasmids were mixed in a 3:1 weight ratio of total plasmid DNA:PEI in 4 mL of either OptiMeM™ (Gibco cat. # 31985-062) or serum-free DMEM per flask and then shaken vigorously for 30 sec ...
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bioRxiv - Microbiology 2020Quote: ... Cells were infected in a BSL-3 lab with the UF-1 strain of SARS-CoV-2 at MOI of 4 in media containing 3% low IgG FBS (Fisher Scientific, Cat. SH30070.03).
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bioRxiv - Immunology 2023Quote: ... Cells were washed 3 times with 200 µL PBS for 4 min before secondary antibodies and 1 µg/mL Hoechst (Thermo Fisher Scientific #33342) were added in 35 µL per well and incubated for 1 hour at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cell layers are examined for cell morphology using a phase contrast microscope washed with media and then 10 uM of 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in the presence or absence of 100 nM insulin as the initiating step and incubated for 20 or 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... and lentiviral vector expressing GFP-UMOD-WT or GFP-UMOD-H177- R185 del at a ratio of 3:1:4 using Lipofectamine 3000 Reagent (ThermoFisher Scientific, Waltham, MA) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and the packaging plasmids psPAX2 and pMD2.G at a ratio 4:3:1 in the presence of Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA). The supernatants containing lentiviral particles were collected 48 hours after transfection ...
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bioRxiv - Biophysics 2024Quote: ... protein fractions were mixed with SDS-free loading buffer in a 3:1 ratio and loaded on a Novex™ 4–20% Tris-Glycine Gels using NativeMark™ Unstained Protein Standards (Invitrogen™) (range from 20 to 1236 kDa ...
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bioRxiv - Immunology 2023Quote: ... The concentration was measured at NanoDrop One/One (Thermo Fisher Scientific) and the quality (RNA integrity number ...
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bioRxiv - Biochemistry 2021Quote: ... and then diluted into fresh YPD for 3-6 hours before inoculating into 96-well plates (Thermo Scientific) at a starting OD600 between 0.04 to 0.1 ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... Reverse 5′-CGA AGG TGT GAC TTC CAT G-3′) on a QuantStudio 6 Flex thermocycler (Applied Biosystems). Standard curve was established in parallel using purified SARS-CoV-2 viral RNA.
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bioRxiv - Biochemistry 2021Quote: ... and then diluted into fresh YPD for 3-6 hours before inoculating into 96-well plates (Thermo Scientific) at a starting OD600 between 0.04 to 0.1 ...
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bioRxiv - Neuroscience 2022Quote: ... the cerebral cortexes from 2-3 P3-P5 C57BL/6 mice were collected in ice cold HBSS (Invitrogen), the tissue was washed three times with HBSS and digested with 0.04% trypsin (Sigma ...
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bioRxiv - Cell Biology 2022Quote: HCT116 cells were cultured in a 6-well plate at 300,000 cells per well in 3 ml McCoy’s 5A medium (Gibco) supplemented with 10% heat inactivated FBS and with 90 UI/mL Penicillin ...
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bioRxiv - Cell Biology 2023Quote: ... X-biotin-PE [N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoylsn-glycero-3-phosphoethanolamine] was obtained from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from 30 ovaries from 3-6 day-old flies using TRIzol (Thermo Fisher Scientific) in three replicates ...
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bioRxiv - Microbiology 2022Quote: ... D-(+)- raffinose or (1% w/v) D-(+)- mannose or or (1% w/v) D-(+)-Xylose in a 96-well microplate (Thermo Scientific, Roskilde, Denmark). Cells were grown with agitation at 37 °C for 18 h in a microplate reader (Tecan (Infinite® 200 PRO ...
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bioRxiv - Cell Biology 2024Quote: ... 15 000 cells/cm2 were plated in a 6-wells plate and after one day transfected using Lipofectamine 2000 (Thermofisher, Cat no. 11668030) in OptiMEM (Gibco ...
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bioRxiv - Molecular Biology 2021Quote: ... antibodies overnight at 4°C and then incubated for an additional 3 hours at 4°C with 50 μL DynabeadsTM protein G (Life Technologies, 10004D). Following three washes with 1% Triton-TBS lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... Fungal hyphae were stained with 0.5 mM 4.4-difluoro-5-methyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid (C1-BODIPY 500/510 C12, Thermo Fisher Scientific) or 4.4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-hexadecanoic acid (BODIPY FL C16 ...
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bioRxiv - Cell Biology 2020Quote: ... 6 or 9 µl of 20 µM siRNA and 4 µl of Lipofectamine RNAi MAX (13778150, Invitrogen) were suspended in 100 µl of water (W4502 ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissue nuclei were visualized with nuclear stain 4′,6-diamidino-2-phenylindole (DAPI, 62247; Thermo Fisher Scientific). Coverslips were mounted using Prolong Gold (P36934 ...
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Prom1 and Notch regulate ciliary length and dynamics in multiciliated cells of the airway epitheliumbioRxiv - Cell Biology 2022Quote: ... 4′,6-diamidino-2-phenylindole (DAPI) was used to stain intracellular DNA (NucBlue, Thermo Fisher, Waltham, MA). Alexa Fluor 647 Phalloidin (Thermo Fisher ...
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bioRxiv - Genomics 2020Quote: ... Sections were stained with DAPI (4′,6′-diamidino-2-phenylindole) and mounted in Prolong Gold (Life Technologies). Images were obtained by confocal microscopy (Nikon C2+ Eclipse ...
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bioRxiv - Neuroscience 2019Quote: ... Tissue was washed in PBS before counterstaining with 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific) and mounting ...
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bioRxiv - Cell Biology 2019Quote: Laurdan (6-dodecanoyl-2-dimethylaminonaphthalene; product D250) and di-4-ANEPPDHQ (product D36802) were purchased from ThermoFisher. Methyl-β-cyclodextrin (product C4555 ...
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bioRxiv - Microbiology 2019Quote: All samples were counterstained with DAPI (4’,6-diamidino-2-phenylindole; Invitrogen, Fisher Scientific AG, Reinach, Switzerland) to visualize nuclei ...
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bioRxiv - Neuroscience 2020Quote: ... the slides were incubated in 1μg/ml of 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific) at RT for 10min ...
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bioRxiv - Cell Biology 2021Quote: ... Sections were mounted with ProLong Diamond Antifade Mountant with 4′,6-diamidino-2-phenylindole (DAPI; Life Technologies) and imaged using a Leica SP5 DMI (zebrafish sections ...
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bioRxiv - Neuroscience 2020Quote: ... The nuclei were stained with 300 nM DAPI (4’,6-diamidino-2-phenylindole; Thermo Fisher Scientific, Slovakia) for 1 min ...
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bioRxiv - Cell Biology 2022Quote: ... Cover glasses were mounted on slides with mountant with 4’,6-diamidino-2-phenylindole (DAPI) (Life Technologies) and imaged with a confocal microscope.
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bioRxiv - Bioengineering 2022Quote: ... cells were stained with antibodies and 500ng/mL 4′,6-diamidine-2′-phenylindole dihydrochloride (DAPI, Invitrogen # D1306). Samples were analyzed on an LSR II flow cytometer (BD).
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bioRxiv - Cell Biology 2022Quote: ... 6 or 9 μl of 20 μM siRNA and 4 μl of lipofectamine RNAi MAX (Invitrogen, 13778150) were suspended in 100 μl of water (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI) staining was used to visualize the nuclei (Thermo Fisher Scientific). Fluorescence was assessed using a fluorescence laser microscope (LSM780 ...
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bioRxiv - Cell Biology 2019Quote: ... 200 μL of PBS containing 1.5 μg/mL DAPI (4’,6-diamidino-2-phenylindole; Thermo Fisher Scientific) was added for 30 min and exchanged to PBS ...
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bioRxiv - Genomics 2019Quote: ... Sections were stained with DAPI (4’,6’- diamidino-2-phenylindole) and mounted in Prolong Gold (Life Technologies). Images were obtained by confocal microscopy (Nikon C2+ Eclipse ...
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bioRxiv - Genomics 2019Quote: ... stained with 4-6-diamidino-2-phenylindole (NucBlue™ Fixed Cell ReadyProbes™ Reagent, R37606, Thermo Fisher) for 5 minutes at room temperature and mounted onto glass slides using ProLong™ Gold antifade reagent (Molecular Probes ...
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bioRxiv - Microbiology 2019Quote: ... cell nuclei were stained with 30 nM DAPI (4′,6-diamidino-2-phenylindole, Invitrogen, Eugene, OR, USA) for 5 min and the coverslips were mounted onto glass slides with CitiFluor AF1 antifading (Electron Microscopy Sciences ...
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bioRxiv - Cancer Biology 2021Quote: ... 4 × 105 cells were seeded in 6-well plates and cultured with RPMI 1640 medium (ThermoFisher # 11875093) containing 10% FBS (Seradigm) ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lines were passaged every 4-6 days and MIN6 were detached with 0.25% trypsin-EDTA (Gibco) and seeded at 7.5-1×106 cells per 75 cm2 flask ...