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Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for Glutathione Reductase Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Plates were developed using 150μl 1-Step ABTS substrate (ThermoFisher, Prod#37615). Following a brief incubation at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Plates were developed using 150μl 1-Step ABTS substrate (ThermoFisher, Prod#37615). Following a brief incubation at room temperature ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... plates were washed 1 × with Neurobasal A with B-27 (Life Technologies) and 0.5 mM Glutamax and replaced with glial conditioned Neurobasal A medium containing B27 ...
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bioRxiv - Physiology 2020Quote: ... Fluorescent signals were obtained by peroxidase-mediated Alexa Fluor 488 tyramide labelling (Alexa fluor 488 Tyramide SuperBoost kit, Thermofisher Scientific, Cat no. B40922). Slides were counterstained with DAPI and mounted in ProLong Diamond antifade mounting medium (Thermofisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... The remaining cells were respectively labelled with aqua-fluorescent reactive dye (dying cells) and calcein violet AM (living cells) using the LIVE/DEAD Violet Viability/Vitality Kit (Molecular Probes, ref. L34958) under manufacturer’s guidelines ...
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bioRxiv - Microbiology 2022Quote: ... and the ratio of viable and dead cells was evaluated using the fluorescent LIVE/DEAD® BacLight™ viability kit (ThermoFisher scientific, USA), as described in our previous works (Nagy et al ...
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bioRxiv - Plant Biology 2024Quote: ... while its concentration was estimated using a fluorescent DNA intercalating agent (e.g., Quant-iT PicoGreen dsDNA Assay Kit, Thermo Fisher Cat. No. P7589) and a microplate reader (Thermo Fisher Scientific)) ...
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bioRxiv - Immunology 2024Quote: ... 1-Step Kit (ThermoFisher Scientific) and StepOne Plus Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2019Quote: ... Stress response was assessed by measuring glutathione levels using 5-chloromethylfluorescein diacetate (Cell tracker™ green CMFDA, Life Technologies) as already shown in mammalian cells [26,87] and yeasts [23,27] ...
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bioRxiv - Cell Biology 2021Quote: ... and GST-tagged baits contained in supernatants were normalized using Pierce Glutathione Magnetic Agarose Beads (Thermo Fisher Scientific, #78602) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Phages presenting NLRP1PYD-specific VHHs were enriched using GST-NLRP1PYD immobilized to magnetic Pierce glutathione beads (Thermo Fisher Scientific). The retained phages were used to infect E ...
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bioRxiv - Neuroscience 2021Quote: ... Lysates were centrifuged at 20,627g for 20 min at 4°C and incubated with glutathione agarose beads (Thermo Scientific) overnight at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... the membrane was then incubated for 1 h in a blocking solution composed of 0.5 % semi-skimmed milk powder in TBST (Tris-buffered saline 20 mM, 0.1 % Tween 20) and then incubated with the anti-glutathione (Invitrogen) primary antibody solution at 4 °C overnight ...
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bioRxiv - Microbiology 2023Quote: ... Anti-GST pull-downs were completed with these protein suspensions subsequently incubated with Pierce™ Glutathione Agarose (ThermoFisher 16100) for an hour at 4°C in binding buffer (PBS pH 7.0 with 1 mM Tris (2-carboxyethyl ...
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bioRxiv - Microbiology 2023Quote: ... GST-RILP or GST proteins were purified by using a pre-equilibrated slurry of glutathione beads (Thermo Scientific, #16100) in TBS containing 1mM MgCl2 and washed three times in the same buffer containing 0.05% Triton X-100 ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was clarified by filtering through a 0.44 ×G μm PVDF syringe filter and then incubated with 2 mL of Pierce glutathione agarose resin (16100, ThermoFisher) for 2 hr at 4 °C in a 5 mL polypropylene column ...
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bioRxiv - Cell Biology 2024Quote: Overexpression of glutathione S-transferase (GST) fusion proteins was induced in DH5α Escherichia coli cells (Life Technologies, MD, USA) using 0.4mM isopropyl β-D-thiogalactopyranoside and cells subsequently broken by sonication ...
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bioRxiv - Cell Biology 2020Quote: ... or on YPD plates (1% yeast extract, 1% peptone and 1% glucose) containing 2% agar supplemented with 100 µg/mL zeocin (Invitrogen), 300 µg/mL hygromycin B (Invitrogen ...
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bioRxiv - Genomics 2021Quote: ... these droplets were transferred at a density of 6 droplets per 1 well of a 6-well plate into STEP3 medium containing a 1:1 mixture of DMEM/F12 (Invitrogen) and Neurobasal with 0.5X N2 supplement (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... Selection of positive transformants was performed on YPD plates (1% Yeast extract, 1% Peptone, and 1% Dextrose) containing 100 μg/ml zeocin (Invitrogen) and/or nourseothricin (Werner Bioagents) ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated with the fluorescent secondary antibodies (Alexa Fluor 568-conjugated anti-rabbit IgG, 1:500, Invitrogen, A11011; 488-conjugated anti-rabbit IgG, Invitrogen A21206) for 3 h at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... and incubated with fluorescent secondary antibody (diluted 1:300 in PBS; goat anti-mouse, Alexa488, Thermo Fisher Scientific, Catalog No. A32723) overnight at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... samples were washed 3 times with PBS and incubated with appropriate fluorescent-labeled secondary antibodies (1:1000 dilution in 0.5% BSA, Life Technologies Inc) and the nuclear marker DAPI (Biolegend) ...
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bioRxiv - Molecular Biology 2021Quote: ... the gels were fixed by 40% ethanol/10% acetic acid for 1 hr and stained overnight in 200 mL of Krypton Fluorescent gel stain (Life Technologies) diluted 1/10 in water ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were washed 4 times for 15 min in PBS-T and then incubated with a fluorescent secondary antibody (1:2000 in PBS-T; goat anti-mouse IgG1 conjugated to Alexa Fluor 488; Invitrogen; RRID:AB_2534069). The tissue was washed twice with PBS ...
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bioRxiv - Developmental Biology 2019Quote: ... and incubated with an antibody that recognizes the Cut antigen (mouse 1:50, DSHB) followed by a fluorescent-conjugated secondary antibody (Alexa Fluor, Molecular Probes). For quantitative purposes ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated in blocking solution for 2 h at room temperature with fluorescent donkey anti-goat alexa fluor 488 (1:500, Invitrogen, A11055) and goat anti-rabbit alexa fluor 647 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated with a green fluorescent conjugated goat anti-mouse Alexa 488 antibody (1/500, Invitrogen™, Waltham, Massachusetts, USA) for 1h30 at room temperature ...
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Observation of an α-synuclein liquid droplet state and its maturation into Lewy body-like assembliesbioRxiv - Molecular Biology 2020Quote: ... overnight at 4 °C and visualised with a fluorescent secondary antibody (goat-anti-mouse IgG Alexa 488-conjugated (1:5000, A11029, Life Technologies)) ...
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bioRxiv - Biophysics 2020Quote: Fluorescent beads were deposited on the coverslip by first preparing a solution with 360 µL water and 1 µl of fluorescent beads (0.1 µm TetraSpeck, T7279, Thermo Fisher Scientific). The solution was vortexed thoroughly ...
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bioRxiv - Cell Biology 2021Quote: ... The 8-well chambers slides were washed three times with PBS 1X and incubated in PBS-Triton/BSA containing fluorescent secondary antibodies at 1/800 (Alexa fluorophores, Life Technologies) for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... ab199376 Abcam)] and 1:500 diluted fluorescent secondary antibody Goat anti-rabbit Alexa Fluor 594 (cat. A11037, Thermo Fisher Scientific, Waltham, MA, USA). Cells were analyzed in a BD FACS Canto flow cytometer with FACS Diva software (Franklin Lakes ...
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bioRxiv - Microbiology 2022Quote: ... and Alexa Fluor 680 fluorescent dye-labeled secondary anti-mouse antibody from goat (diluted 1:10,000, catalogue number A21057; Thermo Scientific, Germany). Quantification of protein bands was performed with Odyssey V3.0 software for Western blots and with ImageLab (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... cells were probed with mouse and rabbit-specific fluorescent-labeled secondary antibodies (1:200, Alexa Fluor 488 and 633, Life Technologies). Single frame images were collected using the Keyence BZ-X 710 Microscope ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibody binding was visualized using species specific fluorescent secondary antibodies conjugated to Alexa Fluor goat anti-mouse 488 (1:1000; Molecular Probes). Sections were mounted using fluorescent mounting medium (DAKO).
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bioRxiv - Biophysics 2022Quote: ... 1.5 μL of blue fluorescent microspheres with a diameter of 1 μm (430/465 nm, F13080, Thermo Fisher Scientific Corp., OR.), used as the markers for correlation between the cryo-SIM 3D and cryo-FIB 2D images ...
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bioRxiv - Microbiology 2019Quote: The viscoelasticity of mucus and agarose were measured by tracking the passive diffusion of 1 μm and 200 nm fluorescent polystyrene beads (F8814 and F8810, ThermoFisher Scientific). To prevent electrostatic or hydrophobic interactions between the beads and the gels ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were incubated for 4 h at room temperature in fluorescent conjugated secondary antibodies (Alexa Fluor 488 goat anti-mouse (Th; 1:500; Invitrogen; A10667) and Alexa Fluor 594 goat anti-rabbit (DsRed ...
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bioRxiv - Genetics 2020Quote: ... hybridization mixtures were created from 5ul of the nick translated fluorescent RNA probe mixed with 10ul of human Cot-1 (ThermoFisher #15279011) and 2ul of Salmon Sperm DNA (ThermoFisher #15632011) ...
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bioRxiv - Bioengineering 2022Quote: ... cameras using python routines in an easy to use framework that builds on Micromanager’s core functionality. We captured our diffraction limited PSF (see Fig. 1D) by bringing a dried 1 μm fluorescent bead (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were serially washed in TBS with the last washing additionally containing fluorescent Nissl stain (1:100 NeuroTraceTM 435/455; ThermoFisher, Germany) in order to identify neuronal cells and anatomical demarcations ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were incubated for 1 hour at room temperature in secondary antibodies conjugated with Alexa Fluor fluorescent probes (Thermo Fisher Scientific) diluted 1:400 in 1% PBS ...
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bioRxiv - Neuroscience 2022Quote: ... and a fluorescent Alexa 594-conjugated goat anti-chicken IgG secondary antibody (1:500; Molecular Probes cat#A-11042, RRID: AB_2534099). Finally ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed with PBS and then incubated in PBS containing 5 % normal goat serum with fluorescent conjugated secondary antibodies (Alexa Fluor 555-conjugated goat anti-mouse IgG, 1:500, Thermo Fisher, A21422 ...
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bioRxiv - Bioengineering 2024Quote: ... 20 µL of 1:100 diluted fluorescent beads in DI water (FluoSpheres carboxylate-modified 0.2 µm, 660/680, F8807, Thermo Fisher Scientific), 6 µL 10% v/v tetramethylethylenediamine (TEMED ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were subsequently visualized with Alexa Fluor fluorescent secondary antibodies (goat anti-rabbit IgG Alexa 519; Thermo Fisher Scientific; 1:1,000) matched to host primary by incubating 2 hours at room temperature ...
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bioRxiv - Cell Biology 2024Quote: The surface roughness of substrates was tested by overlaying a solution of fluorescent beads (1 µm in diameter, F8816, ThermoFisher Scientific). 3.2 µL of the bead stock solution was mixed in 500 µL Matrigel and overlaid onto glass or gel-coated coverslips for 20 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... a streptavidin-Alexa Fluor 594 conjugate (1:500) and 0.4% NeuroTrace 435/455 Blue Fluorescent Nissl stain (Thermo Fisher Scientific; N21479) overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... respectively as described above followed by incubation with a goat anti-rabbit fluorescent secondary antibody (Invitrogen Goat anti-Rabbit Alexa Fluor Plus 568™, Cat# A-11036, 1:1000 or Invitrogen Goat anti-Rabbit Alexa Fluor™ Plus 647 ...
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bioRxiv - Bioengineering 2023Quote: ... 20 µL of 1:100 diluted fluorescent beads in DI water (FluoSpheres carboxylate-modified 0.2 µm, 660/680, F8807, Thermo Fisher Scientific), 6 µL 10% v/v tetramethylethylenediamine (TEMED ...