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Citations for Thermo Fisher :
1401 - 1450 of 10000+ citations for Glutathione Reductase Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... and the electroporation conditions (1350 V, 30ms, 1 pulse) for the delivery and GFP expression was evaluated with EVOS FL fluorescent microscope (Thermo Fisher Scientific).
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Involvement of nucleus accumbens D2-MSN projections to the ventral pallidum in anxious-like behaviorbioRxiv - Neuroscience 2022Quote: ... sections were incubated with the secondary fluorescent antibody Alexa Fluor® 488 donkey anti-goat (1:500, A-11055, Invitrogen, Carlsbad, USA). All sections were stained with 4’,6-diamidino-2-phenylindole (1mg/ml ...
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bioRxiv - Immunology 2020Quote: ... Bacterial DNA was stained using SYTO®41 Blue Fluorescent Nucleic Acid Stain (Molecular Probes #S11352, 1:200 vol/vol, wt/vol) or with SYBR® Green I Nucleic Acid Gel Stain (Invitrogen #S-7563 ...
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bioRxiv - Pathology 2021Quote: ... 1:1000) at 4□ overnight followed by fluorescent staining with Alexa Fluor Plus 647-conjugated donkey anti-rabbit IgG (A32795, Invitrogen, 1:1000) and Alexa Fluor® Plus 555-conjugated donkey anti-mouse antibody (A32773 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stained with the fluorescent lectin concanavalin A (ConA) (100 μg mL-1) conjugated to fluorescein isothiocyanate (FITC) (Invitrogen, United Kingdom) for 5 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... washing buffer containing 500 pM of Atto645 conjugated to DNA-strand I1 and green fluorescent beads (PS-speck, ThermoFisher, dilution 1:1000) was flushed in ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were next washed with PBS three times and incubated with a fluorescent dye-conjugated secondary antibody (1:300; Thermo Fisher Scientific) and phalloidin at 37°C for 2 h ...
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bioRxiv - Bioengineering 2022Quote: ... To differentiate the location of barcoded arrays in the fluorescent image, fluorescein isothiocyanate (FITC, 488 nm emission) conjugated bovine serum albumin (BSA, 1 mg/ml, Thermo Fisher Scientific) in 1x PBS was introduced to the first microfluidic channel ...
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bioRxiv - Cancer Biology 2022Quote: ... They were rinsed with PBS and the cell nuclei were counterstained with Hoechst 33342 fluorescent dye (1/10000, Invitrogen, Carlsbad CA, USA) for 20 min at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... 1h at RT and subsequently incubated overnight at 4°C in primary antibody solution using Rabbit anti-Green-Fluorescent-Protein (GFP; 1:1000 ThermoFisher Scientific, A6455). The following day sections were rinsed three times in PBTriton 0.5% and incubated overnight at 4°C in secondary antibody solution using Goat anti-Rabbit-Oregon Green 488 (1:500 Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were co-transfected at ∼80% confluence with wild-type (4 μg) or mutant SCN1A construct (4 μg) and enhanced green fluorescent protein (eGFP (1 μg) using Lipofectamine 3000 Reagent (Thermo Fisher Scientific) and incubated under the culture conditions above for 24 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed four times in PBS and incubated for 45 min with fluorescent-labeled secondary antibody in blocking solution (1:500, Alexa Fluor 568 Donkey anti-Rabbit IgG, (#A10042, Thermo Fisher Scientific)) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed three times in PBS and incubated for 0.5 h with fluorescent-labeled secondary antibody in blocking solution (1:500, Alexa Fluor 568 Goat anti-Rabbit IgG, (#A11036, Thermo Fisher Scientific)) ...
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bioRxiv - Immunology 2023Quote: ... Cells were then stained with fluorescent secondary antibodies (goat anti-rabbit Alexa Fluor 488, and/or goat anti-mouse Alexa Fluor 488; Invitrogen, 1:1,000), and finally washed with PBS three times for 5 min each ...
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bioRxiv - Cancer Biology 2023Quote: ... Red fluorescent ethidium homodimer (EthD-1) was added at the beginning of the tri-culture to detect dead cells (Invitrogen, Waltham, MA). For the quantification of dead/live cells ...
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bioRxiv - Molecular Biology 2023Quote: ... brain slices were washed 3X in 1X TBS and then incubated for 2 hours at RT with a fluorescent-tagged Alexa Fluor 568 anti-mouse secondary antibody (1:500; Life Technologies A11004). Brain sections were then washed 3X in 1X TBS ...
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bioRxiv - Neuroscience 2023Quote: ... They were then incubated at 4 °C overnight with primary antibody rabbit anti-Myosin VI (1:400 Proteus Bioscience) and mouse anti-green fluorescent protein (Invitrogen, cat. A11120). The tissues were incubated with 1:800 diluted secondary antibodies for 2 hrs at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... washed 3 times with PBT for 10 min at room temperature and incubated with the corresponding secondary fluorescent antibodies (1:100; Thermo Fisher Scientific) in blocking solution for 2 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: BHK-21 and DF-1 cells were plated at 1×105 cells/well in 24-well plates (Nunc). The following day ...
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bioRxiv - Neuroscience 2024Quote: ... and split 1:10-1:20 onto hESC-matrigel coated plates with Y-27632 (10 uM) (Thermo Fisher) in mTeSR1 media.
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bioRxiv - Neuroscience 2023Quote: ... plates in sensory neuronal induction media on day 0 (1:1 mix of Neurobasal (ThermoFisher cat. no. 21103049) and DMEM/F12 medium (ThermoFisher cat ...
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bioRxiv - Cancer Biology 2024Quote: ... Hydrogel-embedded PCLS were gently transferred to culture media in 24-well plates and maintained at 37°C with 5.0% CO2 in DMEM:F12 (1:1; Gibco) media with 0.1% fetal bovine serum (Gibco ...
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bioRxiv - Neuroscience 2024Quote: Mitoprobe JC-1 assay kit (Invitrogen) was used to monitor mitochondrial transmembrane potential using the Mito Probe™ JC-1 Assay Kit protocol ...
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bioRxiv - Microbiology 2019Quote: ... immunoassay plates (96-well plates; Nunc, Rochester, NY, USA) were coated with 100 ng/well of purified antigens at 4°C overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... The plate was applied to a plate magnet (Invitrogen) until the solution cleared and the supernatant was removed without disturbing the beads ...
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bioRxiv - Biochemistry 2019Quote: ... The ELISA plates (Nunc-Immuno Plate; Thermo Scientific, Denmark) were primarily coated with 1 μg TLR2 protein in 100 μl PBS buffer and incubated for 2 h at 37°C ...
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bioRxiv - Neuroscience 2019Quote: ... tissue culture plates (4-well plates; Nunc, Wiesbaden, Germany) were coated with poly-D-lysine (PDL ...
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bioRxiv - Physiology 2019Quote: ... The plate was applied to a plate magnet (Invitrogen) until the solution cleared and the supernatant was removed without disturbing the beads ...
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bioRxiv - Immunology 2022Quote: ... Immunlon 2HB plates 96-well plates (Thermo Scientific 3455) were coated with 50µl of Wuhan-Hu-1 RBD protein (gift of Institute for Protein Design ...
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bioRxiv - Microbiology 2022Quote: ... 96-well microtiter plates (Immulon 2HB plates, Thermo Scientific) were coated with sequentially diluted spRSV/A/Bernett or spRSV/B/BA in PBS (starting at 10 µg/mL or 15 µg/mL of total protein ...
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bioRxiv - Microbiology 2022Quote: ... The plate was applied to a plate magnet (Invitrogen) until the solution cleared and the supernatant was removed without disturbing the beads ...
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bioRxiv - Biochemistry 2022Quote: ... in 96-well white plates (Armadillo plate, Thermo Scientific) using an integration time of 120ms ...
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bioRxiv - Bioengineering 2021Quote: ... before seeding cells was split in two halves and stained independently using cell membrane fluorescent markers from the Vybrant™ Multicolor Cell-Labeling Kit (V22889, Thermo Fisher Scientific Inc., USA). One half was incubated with 5μM DiO Labeling Solution (Green) ...
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bioRxiv - Synthetic Biology 2020Quote: Ten percent of the final RNA amount was used for chemical labelling with Cy3 fluorescent dye using Arcturus Turbo Labeling Cy3 Kit (ThermoFisher, Applied Biosystems, Cat No. KIT0609). The chemical labelling procedure and subsequent removal of free Cy3 dye was performed following manufacturer’s protocol with 15 μg of RNA per reaction ...
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bioRxiv - Immunology 2022Quote: ... for at least 20 min and then stained with FluoroMyelin™ Green fluorescent myelin stain solution or BrainStain™ Imaging Kit (Molecular Probes, Invitrogen, Eugene, OR) diluted 1:300 in PBS for 20 min ...
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bioRxiv - Genomics 2020Quote: ... Microbiopsies were collected in 96-well plates and lysed using the Arcturus PicoPure Kit (Applied Biosystems) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... Amplicons were normalized using the SequalPrep Normalization Plate Kit (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) and eluted in 20 μL Elution Buffer ...
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bioRxiv - Microbiology 2021Quote: ... Each reaction was normalized using the SequalPrep Normalization Plate Kit (Thermo Fisher Scientific, catalog no. A1051001), then pooled and quantified using the Kapa Biosystems Library qPCR MasterMix (ROX Low ...
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bioRxiv - Microbiology 2020Quote: ... The PCR products were purified and normalized by using SequalPrep normalization plate kit (Invitrogen, CA, USA). Pyrosequencing was conducted on an Illumina MiSeq v2.2.0 platform through services provided by the UConn MARS facility.
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bioRxiv - Microbiology 2024Quote: ... They were than pooled and purified with the SequalPrep Normalization Plate kit (Invitrogen, Frederick, MD, USA). Sequencing was performed by GenoScreen (Lille ...
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bioRxiv - Microbiology 2024Quote: ... All samples were then cleaned with SequalPrep Normalization plate kit 96-well (Invitrogen, Carlsbad, CA, USA), pooled and sequenced on MiSeq (Illumina ...
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bioRxiv - Immunology 2024Quote: ... The following day PlatE cells were transfected with pBMN-plasmids using a Lipofectamin 3000 kit (Invitrogen) according to the manufactures protocol ...
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bioRxiv - Systems Biology 2023Quote: Amplicons from each 96-well plate were pooled and purified using the PCR Purification Kit (ThermoFisher). Each pooled sample was prepared as a single sequencing library by the Molecular Biology Core Facilities at the Dana-Farber Cancer Institute ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were purified and normalized using the SequalPrep normalization plate kit (Thermo Scientific, MA, USA) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: GST or GST-PHF1-tudor proteins (10 μg each) were incubated with 4 μl (25% slurry) of Glutathione Magnetic Beads (88821; Thermo Scientific) in 250 μl of binding buffer (125 mM Tris-HCl pH8.0 ...
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bioRxiv - Cell Biology 2019Quote: ... 1 mg clarified mitochondrial lysates prepared in lysis buffer was incubated overnight with 50 µl glutathione-conjugated magnetic agarose beads (Thermo Scientific) at 4°C on a spinning rotor ...
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bioRxiv - Biophysics 2020Quote: ... and unprocessed H57-scFV (still containing a 12x histidine tag) were removed by a batch purification with Ni-NTA agarose (ThermoFischer Scientific) and glutathione agarose (ThermoFisher Scientific). The supernatant containing biotinylated H57-scFV was further purified via gel filtration using Superdex 75 (10/300 GL ...
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bioRxiv - Biophysics 2020Quote: ... Eluted protein fractions were site-specifically biotinylated using the BirA (Acro Biosystems Inc.) and subjected to a batch purification step with glutathione agarose (ThermoFisher Scientific) to remove the BirA ligase and gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences) ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was added to 15 mL of glutathione agarose resin (which binds up to 40 mg of purified recombinant GST protein per milliliter of resin) (Thermo Scientific) and incubated on ice (4°C ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation for 3 hr at 4°C with 250 μg of GST-fusion protein coupled to glutathione-agarose (Thermo Scientific). Beads were then washed four times (pull down buffer containing 0.1% Triton X-100 ...